LOCUS       sequence-489272-       10900 bp    DNA     circular SYN 14-OCT-2025
DEFINITION  A piggyBac vector containing the COX5B genomic sequence, modified 
            with silent mutations to allow for PCR-based analysis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, 
            Ting AY, Weissman JS
  TITLE     Proximity-specific ribosome profiling reveals the logic of localized
            mitochondrial translation.
  JOURNAL   Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 
            10.1016/j.cell.2025.08.002.
  PUBMED    40876456
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002."
COMMENT     Sequence Label: pJL240
FEATURES             Location/Qualifiers
     source          1..10900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    1403..1991
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1509..1520
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1918..1938
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2016..2618)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2102..2122)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2599..2618
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2630..3130)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2693..2711)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     repeat_region   8354..8388
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8879..8895)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8879..8895)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8892..8914)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8903..8919
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8927..8933,8934..8951,8952..8957))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8972..8993
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9110..9127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9281..9869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9361..9380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10040..10831,10832..10900))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10663..10682
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc agggtttatt acagggacag cagagatcca
     1381 gtttggttaa ttagctagcg atgcggggag gcggcccaaa gggagatccg actcgtctga
     1441 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     1501 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     1561 gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg
     1621 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     1681 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     1741 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     1801 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     1861 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     1921 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
     1981 agaggttgat taccgataag cttgatatcg aattcttaac tggcaccggg cttgcgggtc
     2041 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     2101 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     2161 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     2221 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     2281 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     2341 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     2401 acggcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     2461 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
     2521 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
     2581 cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg tggtaccccc tggggagaga
     2641 ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga ggcttgcaga
     2701 atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac cccgcctccc
     2761 gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg ctattggcca
     2821 cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct gcgagggtac
     2881 tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt
     2941 cccgacttag gggcggagca ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga
     3001 tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc
     3061 gtttcccgga accacgccca gagcagccgc gtccctgcgc aaacccaggg ctgccttgga
     3121 aaaggcgcaa acgcgtcaaa ggagacctga gggactgggt gtgagttgac agtggtggga
     3181 gctggaagca gcaagggatg cggggacatc tggggagcct ggacctgacc ttggagctca
     3241 ctcacacaat ctaaataatc ccagagtgag gctgaggttt tcctgagttg gatgagggcc
     3301 tgcgaaaatt tcctattatt aggcaagcct actatgagag tgagaagcct aaactcaatt
     3361 tccattttat agcctggagt aaaccacttc tggtcaggca gcagggaatc ataacatatc
     3421 agagctgaat acctcactgg tttgctgagg ggcaagttag taggtgttcc tcagaaagga
     3481 ttttgtggtc aattaagaca gggaaactct ggcttaaact gcaggtaaat taaataagag
     3541 gtctttactg caagcctaat cagagccttt attatgctag tgtccatctc caaaagggtg
     3601 gtcatcaaat gcaggccctc cctgtcttga ccaagtcctt tttcttgaga tcatgtcttg
     3661 agacttggat gccatgcaat acactttggg aaatactaac ctagttgaac gacagtttta
     3721 ctgatgtaga aatggcggcc caaaggataa aaagtagctg aactagctgg tggggctact
     3781 ttccagacca atgctttttt ctcctgccta agaccttaac cacagttgca aattaactac
     3841 agatcctcag aattttcgcc caattcaact tggaggggtt gcagtggagg tgagggagaa
     3901 gggagtaaca gtgaagccat tcaaagcaaa aaccaccttt aaagaaattt tggccttaga
     3961 atacagggtt tttttttttt ttttttaatt ggtggagggg gcacccagga agaccagacg
     4021 tgctcctgtg aagcaacgct ccaccacgtc cttttgaaaa taccgtagcc tgcatgagac
     4081 aggcgggagc acctaagcct tcaactctcc ccatgttgcc tccaattgac ctcactgggc
     4141 caggaaaagc cctataaact ggttttgtat actctatcca agtagtaaat ttactaaaat
     4201 tactggatcc tactatatac cagacctctg caaggcattg aagattcaat gatgagcatt
     4261 aattcccact ctaaggggat ttttctatct ggtagggaaa ctcagatacc gcatgcagct
     4321 agcctgtcac agggctatac tgactaaagc tcaccctaag aaatttgcac atcttagcct
     4381 atgggcaacg ggaagccatg gacggaatat gttttccgtt tcaaacaagg aactccagtc
     4441 acagcaggct ctaactccat ttcctattat caactcccct tcctgctcct cctcccttag
     4501 tagcctcgaa aaagagtggc cacttccttc acatcaccag ggccttggtc ctggtagggg
     4561 gtcccgcccc cggccctgtc ctcctcccca ggccgggacc tccgcgcctc ccacacacag
     4621 tacaactcta acacgaaact gggccaggac tgcaggtagg tcccgtccaa tgcgacacgc
     4681 tcgcgacggc ccacgcactg ccagaaccag ccctgccact aaccagccta gtaaggaacg
     4741 cagtgatctt taggagccca cttctaaatt caaatcattt ggggttcagt aagtctcttc
     4801 acagacgggg acctaatagc ttccattagc agcctccacg tttgctgaac acgactacgg
     4861 ggccagaccc acaggaactc caatgtggtt cagccgctca actccaggga ccaaaaagac
     4921 tttcccacct cccagcgtta ttaaaggcaa ccccgccccc aagcctcagc cactacgcgg
     4981 tgcagaaaga ggcagagctg ggcatgcgca atttccgccc ccgccacagt cttgggagtg
     5041 ggcgggctcg agggctggga ggaagccgaa agctgaccga gaggagaaag aagcccgccc
     5101 ccggaagtcc ctcctgtctc tgcagcttgt tcccggaagt tttgctgcta gtcgcggacg
     5161 caatggcttc aaggttactt cgcggagctg gaacgctggc cgcgcaggcc ctgagggctc
     5221 gcggccccag tggcgcggcc gcgatgcgct ccatggcatc tggaggtact cgggtctccg
     5281 ggcgtgccag ggaccagagt gttgccctcc cagggtggtc ccagggcggc aaagcggcgc
     5341 ggctcgtgca gcttctcgag gtcccagtgg ccgctttacg gtccccagtg cctcaggctc
     5401 tgcaggcatc tccctgtaat tctggaccgc tgctcctgcc gctccccgaa ctcactccgc
     5461 tgcgaaagta tcctaaacgg aggtgccggg tgaccttggg agggaccggg gctgccaccg
     5521 ggatggggag gggtccggcc tcccttcaaa cctgcgccca cctcaagcag agtgggttct
     5581 acatgctttt agacaaatgt cgacaaattt gcctcggtgg ttggagaaag aaaagctcat
     5641 aggccgggcg cggtggctca caactgtaat cccagcactt tgggaggccg aggcggacag
     5701 atcccctgag gtcaggagct caagaccagc ctggccaaca tggtaaaacc ccgtttctac
     5761 taaaaataca aaaattagcc gggcgtggtg gcgcgcgcct gtagtcccag ctactcggga
     5821 ggctgaggca ggagaatcgc ttaaacccgg gaggcggagg ttcccgtgag ccaacatcgc
     5881 gccattgcac tccagcctgg gcaacaagag caaaactccg tctcaaaaaa gaaaaaaaaa
     5941 gctcccccga gtgctgccgc ttgtgtggat gggtacttgg tggttcttag gggaccatgg
     6001 atatgagtag cctttaggag cttgtgagcc cgctaaaact tatacagaag tttcggggca
     6061 ccattttcct tgatcatttc tgtttgtagt ttttctatca gtcatttcag tcagcgtcat
     6121 aattcacgtt atcttccttt aggtggtgtt cccactgatg aagagcaggc gactgggttg
     6181 gagagggaga tcatgctggc tgcaaagaag ggactggtaa ggagaaactc ccttctgtgt
     6241 cttctgtgta acttatggcc ttggatgtgt tcatagtggt ctcctctctg ggagtatttg
     6301 atacaggaaa cttggcttgt aggaacagtc ccctgagctt ctggaaggta gggcttattt
     6361 ggcctaatgg ttcaattctt gttttttttg ttttgttttg ttttttgttt tttcttcagg
     6421 acccatacaa tgtactggcc ccaaagggag cttcaggcac cagggaagac cctaatttag
     6481 tccccagcat cagcaacaag agaatagtag gctgcatctg taagtacctc acctctattt
     6541 tttatccact tgcttaatat atcctacaat agtgtgtaag ctgcctcaaa tcttcagtgt
     6601 gtgagtgcat gttggtaagt ttgtctaagg gtcttgacac tctcaagcct cattatgcct
     6661 gatagttcat ccttactgga aagaagcgca gcacagcggt aagactggct cactgggagt
     6721 gcggcatgaa ggagtaccca cccagcaaat atttattgtt atactgcttc tatgccaggc
     6781 atcattttag acactaggga tacataccag aacggaccct gctttgcgtt ctacataggc
     6841 aagggaattg ttagaattta cagtgacctt gatacaaggt cagtttactc ataggtgaac
     6901 tcagagcctc aatttcttca tctgaagaat gggaggaggg cttgaactga tctcttaaga
     6961 tatcaaccat agtcttactt gtgtatcaga gatgtctgag gaaaagaaat tcatgttgaa
     7021 agtctccctt tctaggttgg atgaccataa accacctttt ttccctttta ggtgaagagg
     7081 acaataccag cgtcgtctgg ttttggctgc acaaaggcga ggcccagcga tgcccccgct
     7141 gtggagccca ttacaagctg gtgccccagc agctggcaca ctgagcacct gcactaaatt
     7201 actcaaaatg tgctgtaaag tttcttcttt ccagtaaaga ctagccattg cattggctcc
     7261 ttctcccata gatggctggt cttatttctt acccgtattc tttggtaggc atggaatatg
     7321 cttattttgg gaaaagctgt ctgttaatgc tagcttgcca tccacttact gaaagtgtat
     7381 aaccagtgta tagtgcttag attaataata agaatagatc gacaacccgt aatgcaatga
     7441 atgggaccac ctggtatgag agaaaggggc gggctgagga gtcaggctga caggacttaa
     7501 aatattggct ccatcatttg gctctatctc tgtggacatg ttctctgggg gtcaagtaca
     7561 actacaaaaa ggtaagtacc ttacaaggtg cttaatatgt aaagagctga gtggatagta
     7621 ggtcctcaca gtatcctcat agagacaggg tggccttgtg aagagagttt tggggatttt
     7681 gaagtcttga gggacccagg tgcaaagtaa gaattcatgg ggataccagc agcaaaattc
     7741 agaatatgga acaatttcag aaatcactga ccatgtttct tcaaataaat tgcaaggagt
     7801 ggggggaaaa gctagggaga acatgtggag aaacagataa gggagatacc agtcagcagc
     7861 atgcacacct tgtttagatc ttgatttgaa aacaattata aaggaaaaaa aattgagaag
     7921 aatttgaact ctggatattt gatatgtaga catttaccac cctttcaggt atttatatag
     7981 ttttgttttg ttttttttaa tgcttgagat acgcacagga ataagggagg ggagaaatgc
     8041 caaaaaagcc cactttagct gcttcatacc tgtgacacct tgggacaagt taaaatctca
     8101 aaaccctctt acctacaaaa tggaaatacc ttttcatttt gtgtggagat tagccagagg
     8161 tggcttcagt tactatcttc acaaaattgg tcgaaagtag gtatttcagt gggagctgat
     8221 agagtcagag aaaagcccct tcccctcatc ttgcccatcc tgaagggttt aaatcaagcg
     8281 gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa
     8341 tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt aatctagctg
     8401 catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag ctggcgctat
     8461 ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa
     8521 gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac gtttaccatg
     8581 atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt tcaggccacc
     8641 tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag cccgaagcgc
     8701 atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt gcagattaat
     8761 gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg ctggatgccg
     8821 cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcttggcg taatcatggt
     8881 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8941 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     9001 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     9061 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     9121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     9421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     9481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9721 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    10021 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    10081 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    10141 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    10201 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    10261 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    10321 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    10381 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    10441 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    10501 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    10561 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10621 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10681 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10741 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10801 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10861 aaaagggaat aagggcgaca cggaaatgtt gaatactcat
//
