LOCUS       sequence-489275-       10257 bp    DNA     circular SYN 14-OCT-2025
DEFINITION  A piggyBac vector containing a synthetic reporter containing the 5' 
            and 3' UTRs of COX7A2 flanking a partial mCherry sequence with an 
            intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, 
            Ting AY, Weissman JS
  TITLE     Proximity-specific ribosome profiling reveals the logic of localized
            mitochondrial translation.
  JOURNAL   Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 
            10.1016/j.cell.2025.08.002.
  PUBMED    40876456
REFERENCE   2  (bases 1 to 10257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002."
COMMENT     Sequence Label: pJL361
FEATURES             Location/Qualifiers
     source          1..10257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    1403..1991
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1509..1520
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1918..1938
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2016..2618)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2102..2122)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2599..2618
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2630..3130)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2693..2711)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     repeat_region   7711..7745
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8236..8252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8236..8252)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8249..8271)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8260..8276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8284..8290,8291..8308,8309..8314))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8329..8350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8467..8484)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8638..9226)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8718..8737)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9397..10188,10189..10257))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10020..10039
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc agggtttatt acagggacag cagagatcca
     1381 gtttggttaa ttagctagcg atgcggggag gcggcccaaa gggagatccg actcgtctga
     1441 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     1501 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     1561 gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg
     1621 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     1681 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     1741 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     1801 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     1861 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     1921 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
     1981 agaggttgat taccgataag cttgatatcg aattcttaac tggcaccggg cttgcgggtc
     2041 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     2101 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     2161 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     2221 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     2281 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     2341 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     2401 acggcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     2461 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
     2521 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
     2581 cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg tggtaccccc tggggagaga
     2641 ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga ggcttgcaga
     2701 atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac cccgcctccc
     2761 gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg ctattggcca
     2821 cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct gcgagggtac
     2881 tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt
     2941 cccgacttag gggcggagca ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga
     3001 tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc
     3061 gtttcccgga accacgccca gagcagccgc gtccctgcgc aaacccaggg ctgccttgga
     3121 aaaggcgcaa acgcgttggt gagggacgga ttaactatgt taaatgctgc taatagatgg
     3181 agaaggtgaa aattgagatt gacagtttat ttagcaacct ggaagctatt ggtgattcta
     3241 aacaagagca gtaaaagcct gcccaaaatg ggatcaaaaa aagattagga ggaggagaag
     3301 gagagacaag tataaagaaa tctataggat ggtaacggta atcaaaggag tttgttcgtt
     3361 tgtttttaat ttgggaatgt gttaatagat ggaatgatcc agtaaagggg gaaattgtac
     3421 tgaataacat gcatacaaga atctatgtct actttaggat ctatttaatg tgaattcatg
     3481 gctttttttt tttttttttg atatgaagtc tcactctgtc gcccaggctg gagtgcagtg
     3541 aagcgatctt ggctcactgc aagctccacc ttctgggttc acgccattct cctgcctcaa
     3601 cctcctgagt agctgggact acaggcgccc gccaccacgc ccgccttatt ttttgtattt
     3661 ttagtagaga cagggtttca cggctttagc caagatggtc tggatctcct gacctcatga
     3721 tccgcccgcc tcggcttccc aaagtactgg gattacaggc gtgagccact gcgcctggcc
     3781 tcatggctct ttttggacag ggtcataagc ttcttagatc tgtcacccaa tgtaattaat
     3841 tgatgtattg gttaatactg tctttagcag tgctagggga ttggtaagaa atccacatca
     3901 tctctgcgct tgggaaattt acaatctaga gttggctaag tggagaaaac ataagagagc
     3961 acatacaact aacaaattca gattatggac aatgtactta aaaaaccccc acgccctagc
     4021 tattggcacg tggtaatttt gcccagagtt ttggttatat gcataatcca gaggggtgta
     4081 gaaaaccagg tcctttcagt accgatctaa ggaagacatg aattggggtg tttataaacc
     4141 ttggtgagga agagaatttt aactgggact ggtaacttgg tgggcccatt gacatacagt
     4201 agattgtgaa gaagaaaacc ccaaggatta taaggaatag ttttaatatt actaattttt
     4261 atcctttgct gaaactatgg gtttcttatt tgaaatggca atcaaataat ttgtaagagt
     4321 ttgtagaaag tggaaagatt attgtaatac agggtagata tttgcatttg tacatacttg
     4381 agggtaagta caagaaataa acacttgtaa tggggattac ttaagcaaaa tggatgacac
     4441 cgacagcctt tctgtacaac aaataattgc tgtgcttctc tactccagta atgaatgtag
     4501 tgccctgctg gatctgggac agcaagactt ttgagtccag attcgccatc ccatttcttg
     4561 ttccctaacg tttcctcaga agcgagcgag cctcttccac aatttgggga cactgtggaa
     4621 gaacttcttg cttggggagt tctgcagcag tcgaaggtac aagaatataa aaactggtat
     4681 ctcgacacag ctcttacagc atattctcaa cacaaggaac agtaatgtgc cgcagcaatt
     4741 ccttcggtgg aaaaaactga ctgtatccgc gtaagaggag taattctttt ttgaatttta
     4801 tgaggcttca gagctgtatt tcggccaacc cagtctttgt tcagtttcta gcggcttcaa
     4861 ggttgcccag ttttacgcct tctcgctcaa aacagacact agttttcttt gatagcctgg
     4921 tctcacattg acttcatcta agcttattac attcgtctca taaatcgttt gctttttccc
     4981 cagccttagc tccagggacc agcctactga acatcgaaaa ggtcaggaac gagatcacgg
     5041 gactgagacg gcgagccggc tagagcggat agagccgtac tgccgctcta gtttttcttt
     5101 tgctctctat ggttaggagc gcaagcctct cttcggcccg gaaagattta agttcgtgaa
     5161 tgcatacgca agactcggag gtagttccgg ttccggcgtg gccattttcg ttggtggtgt
     5221 tcagttgtgg cggttgctgg tcagtaacag ccaagatgag cgaggtactc gggtctccgg
     5281 gcgtgccagg gaccagagtg ttgccctccc agggtggtcc cagggcggca aagcggcgcg
     5341 gctcgtgcag cttctcgagg tcccagtggc cgctttacgg tccccagtgc ctcaggctct
     5401 gcaggcatct ccctgtaatt ctggaccgct gctcctgccg ctccccgaac tcactccgct
     5461 gcgaaagtat cctaaacgga ggtgccgggt gaccttggga gggaccgggg ctgccaccgg
     5521 gatggggagg ggtccggcct cccttcaaac ctgcgcccac ctcaagcaga gtgggttcta
     5581 catgctttta gacaaatgtc gacaaatttg cctcggtggt tggagaaaga aaagctcata
     5641 ggccgggcgc ggtggctcac aactgtaatc ccagcacttt gggaggccga ggcggacaga
     5701 tcccctgagg tcaggagctc aagaccagcc tggccaacat ggtaaaaccc cgtttctact
     5761 aaaaatacaa aaattagccg ggcgtggtgg cgcgcgcctg tagtcccagc tactcgggag
     5821 gctgaggcag gagaatcgct taaacccggg aggcggaggt tcccgtgagc caacatcgcg
     5881 ccattgcact ccagcctggg caacaagagc aaaactccgt ctcaaaaaag aaaaaaaaag
     5941 ctcccccgag tgctgccgct tgtgtggatg ggtacttggt ggttcttagg ggaccatgga
     6001 tatgagtagc ctttaggagc ttgtgagccc gctaaaactt atacagaagt ttcggggcac
     6061 cattttcctt gatcatttct gtttgtagtt tttctatcag tcatttcagt cagcgtcata
     6121 attcacgtta tcttccttta ggtggtgctg aagggcgaga tcaagcagag gctgaagctg
     6181 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     6241 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     6301 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     6361 gagctgtaca agtaacttca gtcatcccag caatcgcttg gttcagtttc attcagctct
     6421 ctatggacca gtaatctgat aaataaccga gctcttcttt ggggatcaat atttattgac
     6481 ttgtagtaac tgccaccaat aaagcagtct ttaccatgct ttgtctgatt ttatcacttt
     6541 atgccaataa tttcaacttt tggtcagcct tgtaaatctt aggtaaggat caaagaaatg
     6601 attcttttgc cactaaactt gtgaaagcaa cattattgtg atgttatttt agtgaaattt
     6661 tacttctaac agctcaaaat tagtctcaac ttctgtctca acttgccgtt tgaatgcata
     6721 ttgctttttc cccctaccca ctccagtata tcttggaata tgctagaacc cgggggtagt
     6781 ggaagcagat tatctttggc tcctggggtc tatatttctt tttttttttt ttttctcagc
     6841 tatgtgagag tatgcttaaa tattggatat atcccacagc ttagaattag gctttagtag
     6901 aatggctttg taagatattt cagtatttgt ttttttctac actccgagtg ggttcaggta
     6961 ggtttctctc atcaggaacc aaccaaagca ggacagttcc tgggctcccc agctatcatc
     7021 taatggaccc acaactgttt aacctaaaca gtagttaggt gcttaataaa tggtagccat
     7081 aataggctgc tcataatagg aatgtttcaa aaaggtctat ttagggaaca caaaatccaa
     7141 atagatacag agagtaagaa tcaaaaagaa ttacgatgct gctctaggta gtttgcttca
     7201 gggtttgcag aacccttcct gctttgagag aactatgcat gaagctggaa atttcataga
     7261 cttcaagttt gggactccct tttcctgggt cctgttgtgc atccatgtgg agagtagcat
     7321 cttgtgaacc acttcaggac tttccttcta tatatatcga gattggtaat tcgtcaagag
     7381 atactgattg cctactgtgt cctaactggt tttaggtacc taggaatcac tggtgaacaa
     7441 aagcagattc ttcccctcat atgttataga aaggggtggt aggaaagaga gtttaaaaat
     7501 aaattgcaca gtaggttaaa agataagttc tatggaaaaa gaccaaaggg gagaactcac
     7561 atgggtgtgg ttggttggtt gcagtattaa agttaggata ggcttcaaca ggaaggtcag
     7621 attgtttaaa ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat
     7681 ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc
     7741 tagggttaat ctagctgcat caggatcata tcgtcgggtc ttttttccgg ctcagtcatc
     7801 gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg
     7861 ttgaagcggc atggaaagag tttgccgagg atgactgctg ctgcattgac gttgagcgaa
     7921 aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt aacggtgaac
     7981 tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca cagttccgga
     8041 tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg aactgccgtg
     8101 ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc gaggacgggt
     8161 atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc ggcgggcgcg
     8221 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8281 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8341 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8401 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8461 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8521 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8581 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8641 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8701 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8761 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8821 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8881 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8941 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9001 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9061 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9121 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9181 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9241 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9301 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9361 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9421 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9481 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9541 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9601 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9661 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9721 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9781 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9841 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9901 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9961 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10021 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10081 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10141 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10201 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcat
//
