LOCUS       sequence-489301-        9747 bp    DNA     circular SYN 15-OCT-2025
DEFINITION  A piggyBac vector containing a synthetic reporter with 
            mNeonGreen2(11).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9747)
  AUTHORS   Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, 
            Ting AY, Weissman JS
  TITLE     Proximity-specific ribosome profiling reveals the logic of localized
            mitochondrial translation.
  JOURNAL   Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 
            10.1016/j.cell.2025.08.002.
  PUBMED    40876456
REFERENCE   2  (bases 1 to 9747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002."
COMMENT     Sequence Label: pJL296
FEATURES             Location/Qualifiers
     source          1..9747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    1403..1991
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1509..1520
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1918..1938
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2016..2615)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2102..2122)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             complement(2616..2669)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(2679..3386)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2954..2973)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3223..3241
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(3398..3898)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3461..3479)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5958..5987
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     repeat_region   7201..7235
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7726..7742)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7726..7742)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7739..7761)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7750..7766
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7774..7780,7781..7798,7799..7804))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7819..7840
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7957..7974)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8128..8716)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8208..8227)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8887..9678,9679..9747))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9510..9529
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc agggtttatt acagggacag cagagatcca
     1381 gtttggttaa ttagctagcg atgcggggag gcggcccaaa gggagatccg actcgtctga
     1441 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     1501 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     1561 gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg
     1621 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     1681 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     1741 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     1801 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     1861 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     1921 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
     1981 agaggttgat taccgataag cttgatatcg aattcttaac tggcaccggg cttgcgggtc
     2041 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     2101 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     2161 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     2221 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     2281 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     2341 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     2401 acggcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     2461 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
     2521 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
     2581 cgggtggcga ggcgcaccgt gggcttgtac tcggttgggc caggattctc ctcgacgtca
     2641 ccgcatgtta gcagacttcc tctgccctct ccactgccct tgtacagctc gtccatgccg
     2701 ccggtggagt ggcggccctc ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg
     2761 tgggaggtga tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc
     2821 ttggccttgt aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc
     2881 ctctgcttga tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc
     2941 tcccagccca tggtcttctt ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc
     3001 agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc
     3061 acgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc
     3121 ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac
     3181 tgaggggaca ggatgtccca ggcgaagggc agggggccac ccttggtcac cttcagcttg
     3241 gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg
     3301 ccgttcacgg agccctccat gtgcaccttg aagcgcatga actccttgat gatggccatg
     3361 ttatcctcct cgcccttgct caccatggtg gtaccccctg gggagagagg tcggtgattc
     3421 ggtcaacgag ggagccgact gccgacgtgc gctccggagg cttgcagaat gcggaacacc
     3481 gcgcgggcag gaacagggcc cacactaccg ccccacaccc cgcctcccgc accgcccctt
     3541 cccggccgct gctctcggcg cgccctgctg agcagccgct attggccaca gcccatcgcg
     3601 gtcggcgcgc tgccattgct ccctggcgct gtccgtctgc gagggtacta gtgagacgtg
     3661 cggcttccgt ttgtcacgtc cggcacgccg cgaaccgcaa ggaaccttcc cgacttaggg
     3721 gcggagcagg aagcgtcgcc ggggggccca caagggtagc ggcgaagatc cgggtgacgc
     3781 tgcgaacgga cgtgaagaat gtgcgagacc cagggtcggc gccgctgcgt ttcccggaac
     3841 cacgcccaga gcagccgcgt ccctgcgcaa acccagggct gccttggaaa aggcgcaaac
     3901 gcgtcccaaa cagtcattgt actacgcccc taggaaaaaa atataaatac cataccagta
     3961 tctgccttaa gagtttgatc tagtgggaaa ggcatactca tatttaatta taaataatgt
     4021 ggtataagtt ttaaggagtg agtaggcaga caggcaacag ggtagttggt aggcagagga
     4081 atattttggc cccaaactgc cactctaaaa ccttgtcaga ggtaacaaac agcatgcaca
     4141 agtgtttata gagcagccca gggatgggct agaaaagtgg gcaggactgg accacagaag
     4201 ggctttgtat ggtgtgtaag catttaggct atcctgagga gcgtaaatga aagatttgaa
     4261 gcaaaagaaa catttagctg atttgatttt tagatacacc aaaatggagt ctacatggag
     4321 gatggattta agacattaac ttgaggcagg aatttggttg tataacataa acacactttt
     4381 aaaaagttat gtggcctttc tttacggagt acttcatggc tctttattcc atctccctca
     4441 tcaattatat caaaggttag gatggaaagg ctaagacacg gcaatgtctt atttgacaac
     4501 ccctccccaa acccctactc caggctttcc cacagcaatt tcaaaacctt tttcctggca
     4561 cttaacatat tctcccctgc atttgctttt gactgtacag tacataggtc ttaggtctcc
     4621 agtctgaaaa ctgcaccact agattttaca gttccacagg acttagcaca acgtctttga
     4681 aaaaaaaaaa tactgcttaa ataactctca gatctaaatg tataatccta atctcttgat
     4741 tgccagacca cattcccaat tgcccagata tctctacctg gatgtcctat aattatctca
     4801 aacttaacct aatccaaatg gacctgggta cctctcttca ccgccaaact agtcatgctt
     4861 ccatttcata tttaagtact tttccaaacc ctgagtcatc tttgttcact ctctactacc
     4921 ggccatgaac actcagctgc taaatgctgc acacatctct tccatttctc tctcttttcc
     4981 attaccctag tttaagctct tgaaatgctt ccctacctct ccctatttcc cacaaaacct
     5041 acataatagc tatcaggatt accttgctaa agcagaggtg tgatcactgt catttcccac
     5101 cttcaggtag ctggaaaccc cacaaaacga ggatcatgcc catttaaatc ccgcagcatt
     5161 ctccctctcg ttctactgat gccgactgga tcattgtact tgccaagtac agtttgtttt
     5221 tctccccatt acttgtgttt tctagtttct ccaccagatg gtgatgacag tacaactggg
     5281 agctttgtgc cctgtgttcc taggccagtg ccaggcccac ttgaggcgct cactaactat
     5341 gatatgaacg aatgaatgct tgagtgaatt ggtccccaga agcggtcagc ctctccgcca
     5401 aggcagaggg gaagggccgg gaggtgcacg tgggatgaat ggaaacatag tgattggcct
     5461 ttttggagtt ttcttcttaa aaaactgaat acattacaga tgttgaaaac agcacaaaaa
     5521 tcataactgt acggcttgtt aatttttaca aataaataca ccctcgtgaa tccagaacct
     5581 agatcaagaa ccagagcatt accagcaccc agaaacctcc ccgcgccctc ctccactgag
     5641 cggccctttc aacccttaca atgccggagg gcgcaattcc tgggtcctgt gaccttggtg
     5701 accgacgccc gggacaagtc agaaagaccc aagggcgtgg aaaacgctcc tcttaccgat
     5761 aaacttgaag cgactcatga ctgcgcatgg aggacctccc cagcggaagc ggaagtggcc
     5821 gccggcaact ccgcccttcc ggctggcccc gctcagtcac ccgcagcagg cgtgcagttt
     5881 cccggctctc cgcgcggccg gggaaggtca gcgccgtaat gagcgaggtg gtgcacatcc
     5941 gaggaggcgg gggtagtgag cagaaactca tctctgaaga ggatctggga agcggctccg
     6001 gtagcgggag cggatccacc gagctcaact tcaaggagtg gcaaaaggcc tttaccgata
     6061 tgatgtgatt aattaaaaag agagagacct catctttcat gcttgcaagt gaaatatgtt
     6121 acagaacatg cacttgccct aataaaaaat cagtgaaatg gtctctggta tgactgacgt
     6181 tcttctaaga attactgtga tgccaagtga ctgtgccact gtcattgttt tatcaatcgt
     6241 gcatgtaaag aaaatgacag gacatgtagt cagtcagctg tgttctctgc aataaatcac
     6301 tgatagcata tgttaccagt aaggaaaaac tgaggcagaa ccagattgag gcgaacaaac
     6361 tctcaagtca aaggcctaat tagctgtggt aactagaagg aataaacaaa gccaagaaca
     6421 gaaatactga ggatttaaaa ataacctttt tggttttcta aagatttcac taatggaact
     6481 tagtttttat tagaagaaca actctcccta agaagtacat agtcaacaaa taagcatgga
     6541 atttgtggag ttctgtcagg ggtggaggtt tgatggttgg ggttaaatgg tccaatctgg
     6601 gataaaactt tgtggcgctt attttagaag cagttttcca gctttgcttc tgctaccgta
     6661 gcttggattg tctgcggact caaaggtaaa ggaataacag aactccttaa atgtttcaga
     6721 agctttgttt ttgtttttta aagataaagc agtttttaaa actttcaacc caaaacagct
     6781 gcttttagtt cttgatccct cattagctag aactgttttc aaatagtttc aggcaactaa
     6841 tgatcttata gccatcacaa aaatattttg tcacttttct acgtaagctc aatttgagtc
     6901 ccagtccatc ttctaacatt tgactaagac ctcaaactga attgtaacaa gacttgaaat
     6961 acaggacttt tttaatctag tggttcttta tttataacgt aggctgaagc aattgttaca
     7021 atgtaggagg gagacacatt acacaattgt tatttataca agtttagaac aaaaaactgc
     7081 acagggagag gtcaactctc agtacaaact agcgtttaaa ccaagcggcg actgagatgt
     7141 cctaaatgca cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg
     7201 catgcgtcaa ttttacgcag actatctttc tagggttaat ctagctgcat caggatcata
     7261 tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg
     7321 aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag tttgccgagg
     7381 atgactgctg ctgcattgac gttgagcgaa aacgcacgtt taccatgatg attcgggaag
     7441 gtgtggccat gcacgccttt aacggtgaac tgttcgttca ggccacctgg gataccagtt
     7501 cgtcgcggct tttccggaca cagttccgga tggtcagccc gaagcgcatc agcaacccga
     7561 acaataccgg cgacagccgg aactgccgtg ccggtgtgca gattaatgac agcggtgcgg
     7621 cgctgggata ttacgtcagc gaggacgggt atcctggctg gatgccgcag aaatggacat
     7681 ggataccccg tgagttaccc ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc
     7741 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7801 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7861 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7921 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7981 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8041 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8101 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8161 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8221 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8281 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8341 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8401 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8461 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8521 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8581 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8641 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8701 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8761 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8821 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8881 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8941 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9001 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9061 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9121 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9181 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9241 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9301 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9361 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9421 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9481 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9541 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9601 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9661 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9721 ggcgacacgg aaatgttgaa tactcat
//
