LOCUS       sequence-489311-       13567 bp    DNA     circular SYN 15-OCT-2025
DEFINITION  Stably expresses a chimeric protein, ALDH18A1(1-97aa)-SUPV3L1 
            (108aa-358aa)-ALDH18A1 (351-795aa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13567)
  AUTHORS   Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, 
            Ting AY, Weissman JS
  TITLE     Proximity-specific ribosome profiling reveals the logic of localized
            mitochondrial translation.
  JOURNAL   Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 
            10.1016/j.cell.2025.08.002.
  PUBMED    40876456
REFERENCE   2  (bases 1 to 13567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002."
COMMENT     Sequence Label: pJL233
FEATURES             Location/Qualifiers
     source          1..13567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2610..3198
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2716..2727
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3125..3145
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3223..3825)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3309..3329)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3806..3825
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3837..4337)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3900..3918)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        4424..5602
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4655..5593
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5550..5570
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     LTR             9372..9552
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9578..9595)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9584..9808
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9854..10282
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9941..9960)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10151..10172
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10291..10311)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10296..10625
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10476..10611
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10538..10557
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10673..10720
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10739..11113
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11243..11364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11280..11299)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11334..11353
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11413..11429)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11413..11429)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11426..11448)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11437..11453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11461..11467,11468..11485,11486..11491))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11506..11527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11644..11661)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11815..12403)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11895..11914)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12574..13365,13366..13434))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13197..13216
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13435..13539)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagcgatg cggggaggcg gcccaaaggg agatccgact
     2641 cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga
     2701 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
     2761 ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac
     2821 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     2881 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
     2941 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
     3001 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
     3061 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
     3121 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
     3181 gtaatccaga ggttgattac cgataagctt gatatcgaat tcttaactgg caccgggctt
     3241 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
     3301 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
     3361 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
     3421 ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg
     3481 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
     3541 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
     3601 gaccgccacg gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
     3661 cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt
     3721 gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac
     3781 gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatggtgg taccccctgg
     3841 ggagagaggt cggtgattcg gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc
     3901 ttgcagaatg cggaacaccg cgcgggcagg aacagggccc acactaccgc cccacacccc
     3961 gcctcccgca ccgccccttc ccggccgctg ctctcggcgc gccctgctga gcagccgcta
     4021 ttggccacag cccatcgcgg tcggcgcgct gccattgctc cctggcgctg tccgtctgcg
     4081 agggtactag tgagacgtgc ggcttccgtt tgtcacgtcc ggcacgccgc gaaccgcaag
     4141 gaaccttccc gacttagggg cggagcagga agcgtcgccg gggggcccac aagggtagcg
     4201 gcgaagatcc gggtgacgct gcgaacggac gtgaagaatg tgcgagaccc agggtcggcg
     4261 ccgctgcgtt tcccggaacc acgcccagag cagccgcgtc cctgcgcaaa cccagggctg
     4321 ccttggaaaa ggcgcaaatc gattgcaaag atggataaag ttttaaacag agaggaatct
     4381 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     4441 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4501 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4561 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4621 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     4681 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     4741 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     4801 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     4861 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     4921 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     4981 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     5041 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     5101 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     5161 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     5221 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     5281 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     5341 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     5401 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     5461 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     5521 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     5581 tcttccattt caggtgtcgt gacctgcagg atcgtgcggg acgtggacgg aagaaaaaag
     5641 agagtgagcg agcgccggaa tcagcccggc gtggagtgcg gacccgcggt agtgccaggg
     5701 ggcgaaggcg gcggtggtga ggaagatact ttggttagtg accacatcgc agcatgttga
     5761 gtcaagttta ccgctgtggg ttccagccct tcaaccaaca tcttctgccc tgggtcaagt
     5821 gtacaaccgt cttcagatct cattgtatcc agccttcagt catcagacat gttcgttctt
     5881 ggagcaacat cccgtttatc actgtacccc tcagtcgtac acatggcaag tccttcgccc
     5941 accgcagtga gctgaagcat gccaagagaa tcgtggtgaa gctcggcagt gccgtggtga
     6001 cccgagggga tgaatgtggc ctggccctgg ggcgcttggc atctaaactg ggtgctgatt
     6061 atggacttga tgctcgtctc ttccaccaag ctttcataag ctttagaaat tatattatgc
     6121 agtctcattc cctggatgtg gacattcaca ttgttttgaa tgatatttgc ttcggtgcag
     6181 ctcatgcgga tgatttattc ccatttttct tgagacatgc caaacaaata tttcctgtgt
     6241 tggactgtaa ggatgatcta cgtaaaatca gtgacttaag aataccacct aactggtacc
     6301 cagatgctag agccatgcag cggaagataa tatttcattc aggccccaca aacagtggaa
     6361 agacttatca cgcaatccag aaatacttct cagcaaagtc tggagtgtat tgtggccctc
     6421 taaaattact ggcacatgag atcttcgaaa agagtaatgc tgctggtgtg ccatgtgact
     6481 tggtgacagg tgaagagcgt gtgacagttc agccaaatgg gaaacaggct tcacatgttt
     6541 cttgtacagt tgagatgtgc agtgttacaa ctccttatga agtggctgta attgatgaaa
     6601 ttcaaatgat tagagatcca gccagaggat gggcctggac cagagcactt ctaggactgt
     6661 gtgctgaaga ggttcatttg tgtggagaac ctgctgctat tgacctggtg atggagctta
     6721 tgtacacaac gggggaggaa gtggaggttc gagactataa gaggcttacc cccatttctg
     6781 tgctggacca tgcactaacc ttcttttcag aagtaaagcc tgcaggccct actgttgagc
     6841 agcagggaga aatggcgcga tctggaggaa ggatgttggc caccttggaa cctgagcaga
     6901 gagcagaaat tatccatcat ctggctgatc tgttgacgga ccagcgtgat gagatcctgt
     6961 tagccaacaa aaaagacttg gaggaggcag aggggagact tgcagctcct ctgctgaaac
     7021 gtttaagcct ctccacatcc aaattgaaca gcctggccat cggtctgcga cagatcgcag
     7081 cctcctccca ggacagcgtg ggacgtgttt tgcgccgcac ccgaatcgcc aaaaacttgg
     7141 aactggaaca agtgactgtc ccaattggag ttctgctggt gatctttgaa tctcgtcctg
     7201 actgtctacc ccaggtggca gctttggcta tcgcaagtgg caatggcttg ttactcaaag
     7261 gagggaagga ggctgcacac agcaaccgga ttctccacct cctgacccag gaggctctct
     7321 caatccatgg agtcaaggag gccgtgcaac tggtgaatac cagagaagaa gttgaagatc
     7381 tttgccgcct agacaaaatg atagatctga tcattccacg tggctcttcc cagctggtca
     7441 gagacatcca gaaagctgct aaggggattc cagtgatggg gcacagcgaa gggatctgtc
     7501 acatgtatgt ggattccgag gccagtgttg ataaggtcac caggctagtc agagactcta
     7561 aatgtgaata tccagctgcc tgtaatgctt tggagacttt gttaatccac cgggatctgc
     7621 tcaggacacc attatttgac cagatcattg atatgctgag agtggaacag gtaaaaattc
     7681 atgcaggccc caaatttgcc tcctatctga ccttcagccc ctccgaagtg aagtcactcc
     7741 gaactgagta tggggacctg gaattatgca ttgaagtagt ggacaacgtt caggatgcca
     7801 ttgaccacat ccacaagtat ggcagctccc acacggatgt catcgtcaca gaggacgaaa
     7861 acacagcgga gttcttcctg cagcacgtag acagtgcctg tgtgttctgg aatgccagca
     7921 ctcgcttttc tgatggttac cgctttggac tgggagctga agtgggaatc agtacatcga
     7981 gaatccacgc ccggggacca gtaggacttg agggactgct tactactaag tggctgctgc
     8041 gagggaagga ccacgtggtc tcagatttct cagagcatgg aagtttaaaa tatcttcatg
     8101 agaacctccc tattcctcag agaaacacca actgattagt ctgctgaaac aagccgcctg
     8161 aacgcgttaa gtcgacaaag agccaggaaa acccgggaat tttccaaaag gtcttcacgt
     8221 taaacttgtc ttatctcagg agagagcccg ctcttgtctc ccagttcctg gtagggtctg
     8281 cctgttggaa agtgtacctg gatgcttctg ggctccgttt ggcaatagca gtcttggctg
     8341 atgtgcacag tctggctccc agctcaccct ttttttttaa agtaagaaaa tagttgctac
     8401 cgatagggac tttgccaagt ccaattatct tctaggattg aaaggtgcat tttccccata
     8461 aaaaaggcga ggaaaaccca tggctgcttt gtgtcacctc agtgacttac agtccccctt
     8521 ggcatttagt tggtactaga gccagtcatc cttaacaaat cttttcacat tttatttctt
     8581 tcacatgtag tcatcttcaa aaaggaaaga tttggaattt tagaaaaggg gcaactcttc
     8641 tttttagcat tctcatcaga aagtcacaaa aatcgatgga atcatttcca ctgggaagat
     8701 tgaccttttg tatttatttg tggggtaaat taataagcat tccagatgct tgcagcttcc
     8761 tgcatccagg agatgctgtg ttccccgtga tgcagctgga acccaagctg cagcaggaga
     8821 tgcaagtttc aggatgttcc ccactgagct ggaggaatat ctacagcagt gatgcttgaa
     8881 atttttgtat gaattatttt gtcgtcctac ccttttcctc caaaacaaaa attagaggat
     8941 tattttaata ctttggattc ttcccccttt tttgagatta attaacttta agaccaatga
     9001 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     9061 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     9121 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     9181 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     9241 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     9301 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     9361 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9421 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9481 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9541 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     9601 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     9661 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     9721 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     9781 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     9841 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     9901 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     9961 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    10021 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    10081 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    10141 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    10201 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    10261 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
    10321 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
    10381 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    10441 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
    10501 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
    10561 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
    10621 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
    10681 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
    10741 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
    10801 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
    10861 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
    10921 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
    10981 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
    11041 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
    11101 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
    11161 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
    11221 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
    11281 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    11341 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
    11401 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    11461 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    11521 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    11581 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    11641 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    11701 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    11761 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    11821 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    11881 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    11941 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    12001 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    12061 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    12121 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    12181 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    12241 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    12301 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    12361 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    12421 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    12481 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    12541 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    12601 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    12661 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    12721 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    12781 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    12841 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    12901 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    12961 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    13021 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    13081 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    13141 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    13201 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    13261 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    13321 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    13381 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    13441 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    13501 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    13561 acctgac
//
