LOCUS       sequence-489314-       13600 bp    DNA     circular SYN 15-OCT-2025
DEFINITION  Stably expresses fusion of SUPV3L1(1-114aa) and ALDH18A1(106-795aa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13600)
  AUTHORS   Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, 
            Ting AY, Weissman JS
  TITLE     Proximity-specific ribosome profiling reveals the logic of localized
            mitochondrial translation.
  JOURNAL   Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 
            10.1016/j.cell.2025.08.002.
  PUBMED    40876456
REFERENCE   2  (bases 1 to 13600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002."
COMMENT     Sequence Label: pJL178
FEATURES             Location/Qualifiers
     source          1..13600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2610..3198
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2716..2727
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3125..3145
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3223..3825)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3309..3329)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3806..3825
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3837..4337)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3900..3918)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        4424..5602
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4655..5593
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5550..5570
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     LTR             9405..9585
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9611..9628)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9617..9841
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9887..10315
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9974..9993)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10184..10205
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10324..10344)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10329..10658
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10509..10644
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10571..10590
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10706..10753
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10772..11146
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11276..11397
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11313..11332)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11367..11386
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11446..11462)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11446..11462)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11459..11481)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11470..11486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11494..11500,11501..11518,11519..11524))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11539..11560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11677..11694)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11848..12436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11928..11947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12607..13398,13399..13467))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13230..13249
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13468..13572)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagcgatg cggggaggcg gcccaaaggg agatccgact
     2641 cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga
     2701 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
     2761 ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac
     2821 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     2881 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
     2941 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
     3001 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
     3061 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
     3121 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
     3181 gtaatccaga ggttgattac cgataagctt gatatcgaat tcttaactgg caccgggctt
     3241 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
     3301 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
     3361 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
     3421 ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg
     3481 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
     3541 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
     3601 gaccgccacg gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
     3661 cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt
     3721 gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac
     3781 gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatggtgg taccccctgg
     3841 ggagagaggt cggtgattcg gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc
     3901 ttgcagaatg cggaacaccg cgcgggcagg aacagggccc acactaccgc cccacacccc
     3961 gcctcccgca ccgccccttc ccggccgctg ctctcggcgc gccctgctga gcagccgcta
     4021 ttggccacag cccatcgcgg tcggcgcgct gccattgctc cctggcgctg tccgtctgcg
     4081 agggtactag tgagacgtgc ggcttccgtt tgtcacgtcc ggcacgccgc gaaccgcaag
     4141 gaaccttccc gacttagggg cggagcagga agcgtcgccg gggggcccac aagggtagcg
     4201 gcgaagatcc gggtgacgct gcgaacggac gtgaagaatg tgcgagaccc agggtcggcg
     4261 ccgctgcgtt tcccggaacc acgcccagag cagccgcgtc cctgcgcaaa cccagggctg
     4321 ccttggaaaa ggcgcaaatc gattgcaaag atggataaag ttttaaacag agaggaatct
     4381 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     4441 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4501 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4561 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4621 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     4681 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     4741 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     4801 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     4861 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     4921 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     4981 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     5041 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     5101 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     5161 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     5221 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     5281 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     5341 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     5401 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     5461 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     5521 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     5581 tcttccattt caggtgtcgt gacctgcagg atcgtgcggg acgtggacgg aagaaaaaag
     5641 agagtgagcg agcgccggaa tcagcccggc gtggagtgcg gacccgcggt agtgccaggg
     5701 ggcgaaggcg gcggtggtga ggaagatact ttggttagtg accacatcgc agcatgtcct
     5761 tctcccgtgc cctattgtgg gctcggctcc cggcggggcg ccaggctggc caccgggcag
     5821 ccatctgctc tgcccttcgt ccccactttg ggccctttcc cggggttctg gggcaagttt
     5881 ctgtccttgc caccgcctcc tcctctgcct ccggtggctc caaaatacca aacacgtcct
     5941 tgttcgtgcc cctgactgtg aaacctcagg gccccagcgc cgacggcgac gtcggggccg
     6001 agctaacccg gcctctggac aagaatgaag taaagaaggt cttagacaaa ttttacaaga
     6061 ggaaagaaat tcagaaactg ggtgctgatt atggacagaa tcagggcaga gagatgatgc
     6121 tggtgaccag tggagccgta gcctttggca aacaacgctt gcgccatgag atccttctgt
     6181 ctcagagcgt gcggcaggcc ctccactcgg ggcagaacca gctgaaagaa atggcaattc
     6241 cagtcttaga ggcacgagcc tgtgcagctg ccggacagag tgggctgatg gccttgtatg
     6301 aggctatgtt tacccagtac agcatctgtg ctgcccagat tttggtgacc aatttggatt
     6361 tccatgatga gcagaagcgc cggaacctca atggaacact tcatgaactc cttagaatga
     6421 acattgtccc cattgtcaac acaaatgatg ctgttgtccc cccagctgag cccaacagtg
     6481 acctgcaggg ggtaaatgtt attagtgtta aagataatga tagcctggct gcccgactgg
     6541 ctgtggaaat gaaaactgat ctcttgattg ttctttcaga tgtagaaggc ctttttgaca
     6601 gccccccagg ttcagatgat gcaaagctta ttgatatatt ttatcccgga gatcagcagt
     6661 ctgtgacatt tggaaccaag tctagagtgg gaatgggtgg catggaagcc aaggtgaaag
     6721 cagccctctg ggctttgcaa ggtggcactt ctgttgttat tgcaaatgga acccacccaa
     6781 aggtgtctgg gcacgtcatc acagacattg tggaggggaa gaaagttggt accttctttt
     6841 cagaagtaaa gcctgcaggc cctactgttg agcagcaggg agaaatggcg cgatctggag
     6901 gaaggatgtt ggccaccttg gaacctgagc agagagcaga aattatccat catctggctg
     6961 atctgttgac ggaccagcgt gatgagatcc tgttagccaa caaaaaagac ttggaggagg
     7021 cagaggggag acttgcagct cctctgctga aacgtttaag cctctccaca tccaaattga
     7081 acagcctggc catcggtctg cgacagatcg cagcctcctc ccaggacagc gtgggacgtg
     7141 ttttgcgccg cacccgaatc gccaaaaact tggaactgga acaagtgact gtcccaattg
     7201 gagttctgct ggtgatcttt gaatctcgtc ctgactgtct accccaggtg gcagctttgg
     7261 ctatcgcaag tggcaatggc ttgttactca aaggagggaa ggaggctgca cacagcaacc
     7321 ggattctcca cctcctgacc caggaggctc tctcaatcca tggagtcaag gaggccgtgc
     7381 aactggtgaa taccagagaa gaagttgaag atctttgccg cctagacaaa atgatagatc
     7441 tgatcattcc acgtggctct tcccagctgg tcagagacat ccagaaagct gctaagggga
     7501 ttccagtgat ggggcacagc gaagggatct gtcacatgta tgtggattcc gaggccagtg
     7561 ttgataaggt caccaggcta gtcagagact ctaaatgtga atatccagct gcctgtaatg
     7621 ctttggagac tttgttaatc caccgggatc tgctcaggac accattattt gaccagatca
     7681 ttgatatgct gagagtggaa caggtaaaaa ttcatgcagg ccccaaattt gcctcctatc
     7741 tgaccttcag cccctccgaa gtgaagtcac tccgaactga gtatggggac ctggaattat
     7801 gcattgaagt agtggacaac gttcaggatg ccattgacca catccacaag tatggcagct
     7861 cccacacgga tgtcatcgtc acagaggacg aaaacacagc ggagttcttc ctgcagcacg
     7921 tagacagtgc ctgtgtgttc tggaatgcca gcactcgctt ttctgatggt taccgctttg
     7981 gactgggagc tgaagtggga atcagtacat cgagaatcca cgcccgggga ccagtaggac
     8041 ttgagggact gcttactact aagtggctgc tgcgagggaa ggaccacgtg gtctcagatt
     8101 tctcagagca tggaagttta aaatatcttc atgagaacct ccctattcct cagagaaaca
     8161 ccaactgatt agtctgctga aacaagccgc ctgaacgcgt taagtcgaca aagagccagg
     8221 aaaacccggg aattttccaa aaggtcttca cgttaaactt gtcttatctc aggagagagc
     8281 ccgctcttgt ctcccagttc ctggtagggt ctgcctgttg gaaagtgtac ctggatgctt
     8341 ctgggctccg tttggcaata gcagtcttgg ctgatgtgca cagtctggct cccagctcac
     8401 cctttttttt taaagtaaga aaatagttgc taccgatagg gactttgcca agtccaatta
     8461 tcttctagga ttgaaaggtg cattttcccc ataaaaaagg cgaggaaaac ccatggctgc
     8521 tttgtgtcac ctcagtgact tacagtcccc cttggcattt agttggtact agagccagtc
     8581 atccttaaca aatcttttca cattttattt ctttcacatg tagtcatctt caaaaaggaa
     8641 agatttggaa ttttagaaaa ggggcaactc ttctttttag cattctcatc agaaagtcac
     8701 aaaaatcgat ggaatcattt ccactgggaa gattgacctt ttgtatttat ttgtggggta
     8761 aattaataag cattccagat gcttgcagct tcctgcatcc aggagatgct gtgttccccg
     8821 tgatgcagct ggaacccaag ctgcagcagg agatgcaagt ttcaggatgt tccccactga
     8881 gctggaggaa tatctacagc agtgatgctt gaaatttttg tatgaattat tttgtcgtcc
     8941 tacccttttc ctccaaaaca aaaattagag gattatttta atactttgga ttcttccccc
     9001 ttttttgaga ttaattaact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     9061 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     9121 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     9181 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     9241 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     9301 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     9361 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     9421 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9481 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9541 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc
     9601 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     9661 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     9721 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     9781 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     9841 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     9901 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     9961 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    10021 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    10081 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    10141 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    10201 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    10261 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
    10321 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
    10381 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
    10441 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
    10501 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
    10561 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
    10621 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
    10681 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
    10741 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
    10801 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
    10861 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
    10921 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
    10981 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
    11041 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
    11101 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
    11161 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
    11221 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
    11281 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    11341 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    11401 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
    11461 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    11521 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    11581 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
    11641 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    11701 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    11761 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    11821 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    11881 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    11941 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    12001 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    12061 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    12121 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    12181 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    12241 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
    12301 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    12361 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    12421 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    12481 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    12541 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    12601 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    12661 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    12721 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    12781 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    12841 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    12901 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    12961 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    13021 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    13081 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    13141 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    13201 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    13261 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    13321 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    13381 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    13441 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    13501 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    13561 aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
