LOCUS       sequence-48943-e         684 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RHOU
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg ggcctgggga gccggggggc cgggggcgtg cggggggtgc cgaggggcgc
      121 ggcgtcaagt gcgtgctggt cggcgacggc gcggtgggca agacgagcct ggtggtgagc
      181 tacaccacca acggctaccc caccgagtac atccctactg ccttcgacaa cttctccgcg
      241 gtggtgtctg tggatgggcg gcccgtgaga ctccaactct gtgacactgc cggacaggat
      301 gaatttgaca agctgaggcc tctctgctac accaacacag acatcttcct gctctgcttc
      361 agtgtcgtga gcccctcatc cttccagaac gtcagtgaga aatgggtgcc ggagattcga
      421 tgccactgtc ccaaagcccc catcatccta gttggaacgc agtcggatct cagagaagat
      481 gtcaaagtcc tcattgagtt ggacaaatgc aaagaaaagc cagtgcctga agaggcggct
      541 aagctgctag ccgaggaaat caaagccgcc tcctacatcg agtgttcagc cttgactcaa
      601 aaaaacctca aagaggtctt tgatgcagcc atcgtcgctg gcattcaata ctcggacact
      661 cagcaatgac agtaaaggtg gata
//
