LOCUS       sequence-489436-        9419 bp    DNA     circular SYN 15-OCT-2025
DEFINITION  Overexpression of mutant human FAM136A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Harhai M, Foged MM, Zarges C, Landoni JC, Chollet S, Simonelli M, 
            Recazens E, Lisci M, Laban N, Manley S, Riemer J, Lopez-Escamez JA, 
            Lysakowski A, Jourdain AA
  TITLE     An updated inventory of genes essential for oxidative 
            phosphorylation identifies a mitochondrial origin in familial 
            Meniere's disease.
  JOURNAL   Cell Rep. 2025 Jul 25;44(8):116069. doi: 
            10.1016/j.celrep.2025.116069.
  PUBMED    40714634
REFERENCE   2  (bases 1 to 9419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2025-07-25"; volume: "44(8):116069. doi"; pages: " 
            10.1016/j.celrep.2025.116069"
COMMENT     Sequence Label: pLV-FAM136A-mutant-3xFLAG-hygro
FEATURES             Location/Qualifiers
     source          1..9419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    692..817
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             864..1497
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    1665..1799
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1689..1708)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1743..1762
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1847..1951
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1952..2020,2021..2812)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2170..2189)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2983..3571
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3472..3491
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3725..3742
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3859..3880
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3895..3900,3901..3918,3919..3925)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3933..3949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3938..3960
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3957..3973
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3957..3973
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             4101..4734
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    4941..5529
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5047..5058
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5456..5476
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5553..6578)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(5553..6578)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    6581..7130
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6740..6759)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     6969..6986
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(7240..7305)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          7546..8484
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(7569..7589)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(8485..8696)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    8774..8889
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(9152..9196)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9381..9419,1..195)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc
       61 tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc
      121 tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca
      181 aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc
      241 ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata taattcactt
      301 ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg gccgcttgct
      361 gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt
      421 cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc
      481 taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc
      541 cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc
      601 atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc
      661 atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct
      721 agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg
      781 tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt
      841 tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag
      901 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
      961 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     1021 gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc
     1081 tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag tccccagcgg
     1141 aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga tgcagctctc
     1201 gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact tctctctccg
     1261 ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct cctttattgg
     1321 cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca aaggtcagtg
     1381 gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt
     1441 ggtagatcca cagatcaagg atatcttgtc ttctttggga gtgaattagc ccttccaact
     1501 actaagtttg tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag
     1561 gaatcacagg tttagagtaa atgaaaccac aggtaattgg cagtggtaat agggtatggg
     1621 gtgggaagtt tgggatgatt ttggttagct tgagttatcc agttgatcca gacatgataa
     1681 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     1741 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttg
     1801 atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1861 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1921 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     1981 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     2041 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     2101 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     2161 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     2221 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     2281 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     2341 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     2401 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     2461 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     2521 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     2581 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     2641 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     2701 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     2761 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     2821 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     2881 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     2941 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3001 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3061 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3121 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3181 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3241 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3301 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3361 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3421 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3481 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3541 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3601 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3661 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3721 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     3781 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     3841 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     3901 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     3961 aaacagctat gaccatgatt acgccaagct ctagctagag gtcgacggta aacgctagca
     4021 atgtcaaggc ctctcactct ctgatattca tttctttgca agttataaat actgaataat
     4081 aagatgacat gaactactac tgctagagat tttccacact gactaaaagg gtctgaggga
     4141 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     4201 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     4261 ctggtctaac cagagagacc cagtacaggc aaaaagcagc tgcttatatg caggatctga
     4321 gggctcgcca ctccccagtc ccgcccaggc cacgcctccc tggaaagtcc ccagcggaaa
     4381 gtcccttgta gcaagctcga tatcagcagt tcttgaagta ctccggatgc agctctcggg
     4441 ccacgtgatg aaatgctagg cggctgtcaa acctccactc taacacttct ctctccgggt
     4501 catccatccc atgcaggctc acagggtgta acaagctggt gttctctcct ttattggcct
     4561 cttctacctt atctggctca actggtacta gcttgtagca ccatccaaag gtcagtggat
     4621 atctgacccc tggccctggt gtgtagttct gctaatcagg gaagtagcct tgtgtgtggt
     4681 agatccacag atcaaggata tcttgtcttc gttgggagtg aattagccct tccagtcccc
     4741 tcttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag
     4801 gtacctgagg tgtgactgga aaacccacct cctcctcctc ttgtgcttct agccaggcac
     4861 aatcagcatt ggtagctgct gtattgctac ttgtgattgc tccatgtttt tctaggtctc
     4921 gactgcagaa ttaattccag gcggggaggc ggcccaaagg gagatccgac tcgtctgagg
     4981 gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc
     5041 cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc
     5101 aacacaggcg agcagccatg gaaaggacgt cagcttcccc gacaacacca cggaattgtc
     5161 agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc
     5221 cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat
     5281 gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac
     5341 aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat
     5401 gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag
     5461 cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag
     5521 aggttgattg ttccagacgc gccccgcccg ggctattcct ttgccctcgg acgagtgctg
     5581 gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg
     5641 cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg
     5701 catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt
     5761 tggtcaagac caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga
     5821 tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag
     5881 aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc
     5941 gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg
     6001 tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg
     6061 gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc
     6121 gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg
     6181 aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc
     6241 tgcagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg
     6301 cgggagatgc aataggtcag gctctcgcta aattccccaa tgtcaagcac ttccggaatc
     6361 gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg
     6421 cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat
     6481 tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
     6541 ttctcgacag acgtcgcggt gagttcaggc tttttcatgg tattatcgtg tttttcaaag
     6601 gaaaaccacg tccccgtggt tcggggggcc tagacgtttt tttaacctcg actaaacaca
     6661 tgtaaagcat gtgcaccgag gccccagatc agatcccata caatggggta ccttctgggc
     6721 atccttcagc cccttgttga atacgcttga ggagagccat ttgactcttt ccacaactat
     6781 ccaactcaca acgtggcact ggggttgtgc cgcctttgca ggtgtatctt atacacgtgg
     6841 cttttggccg cagaggcacc tgtcgccagg tggggggttc cgctgcctgc aaagggtcgc
     6901 tacagacgtt gtttgtcttc aagaagcttc cagaggaact gcttccttca cgacattcaa
     6961 cagaccttgc attcctttgg cgagagggga aagaccccta ggaatgctcg tcaagaagac
     7021 agggccaggt ttccgggccc tcacattgcc aaaagacggc aatatggtgg aaaataacat
     7081 atagacaaac gcacaccggc cttattccaa gcggcttcgg ccagtaacgt tagggggggg
     7141 gggcggaatt gatcccgcta gagggaaacc gttgctagct taagttagtt aacaattgtt
     7201 accgcggccc gtcgactcta gagcggccgc tcactattat ttatcatcat catctttata
     7261 atcaatatca tgatctttat aatcgccatc atgatcttta taatcaaatt tttccagctc
     7321 gcttgtaacc aaggcttgtg cttgggcgag aggtacatgg cacctttcaa tgcactgatg
     7381 gacctgtttc atactcgcct gactatcctc acaacaggaa gctgaacacc ggaacatcaa
     7441 gccctgcatt tttctgatgt tctccctttc aagagacttg accattgact ccactgcctc
     7501 ctgtaccctc aattgctgaa gctctgccat ggatcctcac gacacctgaa atggaagaaa
     7561 aaaactttga accactgtct gaggcttgag aatgaaccaa gatccaaact caaaaagggc
     7621 aaattccaag gagaattaca tcaagtgcca agctggccta acttcagtct ccacccactc
     7681 agtgtgggga aactccatcg cataaaaccc ctccccccaa cctaaagacg acgtactcca
     7741 aaagctcgag aactaatcga ggtgcctgga cggcgcccgg tactccgtgg agtcacatga
     7801 agcgacggct gaggacggaa aggccctttt cctttgtgtg ggtgactcac ccgcccgctc
     7861 tcccgagcgc cgcgtcctcc attttgagct ccctgcagca gggccgggaa gcggccatct
     7921 ttccgctcac gcaactggtg ccgaccgggc cagccttgcc gcccagggcg gggcgataca
     7981 cggcggcgcg aggccaggca ccagagcagg ccggccagct tgagactacc cccgtccgat
     8041 tctcggtggc cgcgctcgca ggccccgcct cgccgaacat gtgcgctggg acgcacgggc
     8101 cccgtcgccg cccgcggccc caaaaaccga aataccagtg tgcagatctt ggcccgcatt
     8161 tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt catcaaaaat tttaaatggc
     8221 tagagactta tcgaaagcag cgagacaggc gcgaaggtgc caccagattc gcacgcggcg
     8281 gccccagcgc ccaggccagg cctcaactca agcacgaggc gaaggggctc cttaagcgca
     8341 aggcctcgaa ctctcccacc cacttccaac ccgaagctcg ggatcaagaa tcacgtactg
     8401 cagccaggtg gaagtaattc aaggcacgca agggccataa cccgtaaaga ggccaggccc
     8461 gcgggaacca cacacggcac ttacctgtgt tctggcggca aacccgttgc gaaaaagaac
     8521 gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa aaaggcggag
     8581 ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg caccggttca
     8641 attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct ctgcccactg
     8701 acgggcacct cgagtgaaga cgaaagggcc tcatcgataa actggatctc tgctgtccct
     8761 gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat
     8821 gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt
     8881 tcttttaaag gcgataccgt cgagatccgt tcactaatcg aatggatctg tctctgtctc
     8941 tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt
     9001 gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata gaaagtacag
     9061 caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc
     9121 acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata
     9181 attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc
     9241 ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt
     9301 ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc
     9361 cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagg
//
