LOCUS       sequence-489440-        9608 bp    DNA     circular SYN 15-OCT-2025
DEFINITION  Overexpression of wild-type human FAM136A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Harhai M, Foged MM, Zarges C, Landoni JC, Chollet S, Simonelli M, 
            Recazens E, Lisci M, Laban N, Manley S, Riemer J, Lopez-Escamez JA, 
            Lysakowski A, Jourdain AA
  TITLE     An updated inventory of genes essential for oxidative 
            phosphorylation identifies a mitochondrial origin in familial 
            Meniere's disease.
  JOURNAL   Cell Rep. 2025 Jul 25;44(8):116069. doi: 
            10.1016/j.celrep.2025.116069.
  PUBMED    40714634
REFERENCE   2  (bases 1 to 9608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2025-07-25"; volume: "44(8):116069. doi"; pages: " 
            10.1016/j.celrep.2025.116069"
COMMENT     Sequence Label: pLV-FAM136A-WT-3xFLAG-hygro
FEATURES             Location/Qualifiers
     source          1..9608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    137..725
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(190..210)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(608..619)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             932..1565
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1693..1709)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1693..1709)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1706..1728)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1717..1733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1741..1747,1748..1765,1766..1771))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1786..1807
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1924..1941)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2095..2683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2175..2194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2854..3645,3646..3714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3477..3496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3715..3819)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    3867..4001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3904..3923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3958..3977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4169..4802
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    4849..4974
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5471..5704
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5889..5933
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6196..6311
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        6389..6600
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          6601..7539
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7496..7516
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7969..8034
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8144..8693
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8288..8305)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8515..8534
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8696..9608,1..113)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             join(8696..9608,1..113)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
       61 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagcccgggc
      121 ggggcgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
      181 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
      241 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
      301 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
      361 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
      421 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
      481 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
      541 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
      601 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
      661 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
      721 cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc
      781 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
      841 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
      901 cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga
      961 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
     1021 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
     1081 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
     1141 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
     1201 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
     1261 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
     1321 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
     1381 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     1441 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     1501 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     1561 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     1621 atcagagagt gagaggcctt gacattgcta gcgtttaccg tcgacctcta gctagagctt
     1681 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     1741 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     1801 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     1861 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     1921 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1981 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2041 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2101 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2161 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2221 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2281 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2341 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2401 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2461 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2521 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2581 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2641 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2701 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2761 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2821 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2881 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2941 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3001 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3061 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3121 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3181 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3241 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3301 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3361 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3421 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3481 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3541 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3601 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3661 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3721 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3781 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3841 acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat
     3901 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3961 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
     4021 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
     4081 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     4141 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     4201 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     4261 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     4321 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     4381 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     4441 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     4501 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     4561 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     4621 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4681 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     4741 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     4801 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     4861 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     4921 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     4981 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     5041 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     5101 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     5161 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     5221 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     5281 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
     5341 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     5401 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     5461 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     5521 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     5581 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     5641 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     5701 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     5761 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     5821 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     5881 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     5941 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     6001 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     6061 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     6121 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     6181 ctcgacggta tcgcctttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     6241 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     6301 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttatcg atgaggccct
     6361 ttcgtcttca ctcgaggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     6421 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     6481 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     6541 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     6601 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     6661 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     6721 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     6781 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     6841 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     6901 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     6961 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     7021 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     7081 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     7141 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     7201 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     7261 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     7321 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     7381 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     7441 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     7501 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg atccatggca
     7561 gagcttcagc aattgagggt acaggaggca gtggagtcaa tggtcaagtc tcttgaaagg
     7621 gagaacatca gaaaaatgca gggcttgatg ttccggtgtt cagcttcctg ttgtgaggat
     7681 agtcaggcga gtatgaaaca ggtccatcag tgcattgaaa ggtgccatgt acctctcgcc
     7741 caagcacaag ccttggttac aagcgagctg gaaaaatttc aggataggtt ggctaggtgt
     7801 accatgcact gcaacgataa agcaaaggat tctatcgacg cgggtagcaa ggaactccaa
     7861 gttaagcagc aacttgactc atgcgtaacc aagtgtgtgg atgaccatat gcaccttata
     7921 ccgactatga caaaaaagat gaaagaggct cttcttagta ttggaaaaga ttataaagat
     7981 catgatggcg attataaaga tcatgatatt gattataaag atgatgatga taaataatag
     8041 tgagcggccg ctctagagtc gacgggccgc ggtaacaatt gttaactaac ttaagctagc
     8101 aacggtttcc ctctagcggg atcaattccg cccccccccc ctaacgttac tggccgaagc
     8161 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     8221 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     8281 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     8341 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     8401 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     8461 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     8521 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     8581 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     8641 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatgaa
     8701 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     8761 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     8821 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     8881 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     8941 atttagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     9001 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     9061 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     9121 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     9181 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     9241 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     9301 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     9361 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     9421 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     9481 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     9541 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     9601 gactgtcg
//
