LOCUS       sequence-489443-        3348 bp    DNA     circular SYN 15-OCT-2025
DEFINITION  In vitro transcription of human ENDOG downstream of SP6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Harhai M, Foged MM, Zarges C, Landoni JC, Chollet S, Simonelli M, 
            Recazens E, Lisci M, Laban N, Manley S, Riemer J, Lopez-Escamez JA, 
            Lysakowski A, Jourdain AA
  TITLE     An updated inventory of genes essential for oxidative 
            phosphorylation identifies a mitochondrial origin in familial 
            Meniere's disease.
  JOURNAL   Cell Rep. 2025 Jul 25;44(8):116069. doi: 
            10.1016/j.celrep.2025.116069.
  PUBMED    40714634
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2025-07-25"; volume: "44(8):116069. doi"; pages: " 
            10.1016/j.celrep.2025.116069"
COMMENT     Sequence Label: pTwist Amp High Copy ENDOG-HA
FEATURES             Location/Qualifiers
     source          1..3348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             669..695
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(745..764)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(746..764)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(783..799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(861..877)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(874..896)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1049..1637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1129..1148)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(1754..1778)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(1878..2669,2670..2738))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2501..2520
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2739..2843)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2957..2973
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3061..3077
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3085..3103
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3085..3102
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tgaagagtag agagtcttat gttctctgct acgacccccg gaccagggga gcattgtggg
       61 ttgtggaaca actgagaccc gaacgcctca ggggggatgg agacaggaga gagtgcgatt
      121 tccgggagga tgattctgtg cacgcatatc acagggcgac aaacgcagac tatagaggaa
      181 gcggatttga tcgtgggcat ctggcagctg cagccaacca tcggtggtcc cagaaagcta
      241 tggacgatac cttctatttg tccaatgtgg caccccaggt cccacacctt aaccagaatg
      301 cttggaataa cctcgagaag tactcccgtt ctctgacgcg ttcctaccaa aacgtgtacg
      361 tatgcacagg tcctctcttc ctccccagga ccgaggctga cggcaagagc tacgtcaaat
      421 accaggttat aggcaaaaac cacgtggccg ttcccaccca cttctttaaa gttctgatat
      481 tagaagccgc tggtggacag atcgagctga ggacctatgt tatgccaaat gctcctgtcg
      541 acgaagcaat accgcttgaa cggtttctgg tccccattga gtcaattgaa cgtgcgagcg
      601 ggctgctctt tgtgcctaac attttggcac gggcagggtc cctcaaggcc atcaccgcgg
      661 gaagtaaata cccatacgat gttccagatt acgcttaata gtgaggatcc tctagagtcg
      721 acctgcaggc atgcaagctt gtctccctat agtgagtcgt attagagctt ggcgtaatca
      781 tggtcatagc tgtttcctgt gtgaaggcta ggtggaggct cagtgatgat aagtctgcga
      841 tggtggatgc atgtgtcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaga
      901 gggcacaatc ctattccgcg ctatccgaca atctccaaga cattaggtgg agttcagttc
      961 ggcgtatggc atatgtcgct ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1021 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1081 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1141 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1201 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1261 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1321 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1381 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1441 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1501 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1561 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1621 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctctattca
     1681 acaaagccgc cgtcccgtca agtcagcgta aatgggtagg gggcttcaaa tcgtcctcgt
     1741 gataccaatt cggagcctgc ttttttgtac aaacttgttg ataatggcaa ttcaaggatc
     1801 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1861 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1921 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1981 ggcttaccat ctggccccag tgctgcaatg ataccgcgag agccacgctc accggctcca
     2041 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2101 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2161 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2221 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2281 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2341 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2401 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2461 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2521 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2581 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2641 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2701 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2761 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2821 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccagatac ctgaaacaaa
     2881 acccatcgta cggccaagga agtctccaat aactgtgatc caccacaagc gccagggttt
     2941 tcccagtcac gacgttgtaa aacgacggcc agtcatgcat aatccgcacg catctggaat
     3001 aaggaagtgc cattccgcct gacctttggg taacgccagg gttttcccag tcacgacgtt
     3061 gtaaaacgac ggccagtgaa ttggatttag gtgacactat agaatacgaa ttcgagctcg
     3121 gtaccatgag ggcactgcga gctggcctga cactggcctc aggcgctggc ctgggggccg
     3181 tcgtggaggg ctggaggcgg cgaagagaag atgccagagc tgctcccggg ctcctcggcc
     3241 gattgccggt gctgcctgtg gcggctgccg ccgaactccc tccagtgcct ggcgggccga
     3301 gaggtcctgg cgaactcgcc aaatacggtc tccccggtct cgctcagc
//
