LOCUS       sequence-489461-       13399 bp    DNA     circular SYN 15-OCT-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0912
FEATURES             Location/Qualifiers
     source          1..13399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2823..3033
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2845..3041
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2892..3027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3073..3675
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3073..3092)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3569..3589
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3718..3835
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3973..5151
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4204..5142
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5099..5119
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5162..5171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    10309..10897
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10362..10382)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10780..10791)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10969..11202
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11280..11401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11317..11336)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11371..11390
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11441..11576
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11596..11615)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11597..11615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11625..11642)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11625..11641)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11634..11656)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11783..12238
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11870..11889)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12080..12101
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12264..12368
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12369..12437,12438..13229)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12587..12606)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2821 cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc
     2881 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2941 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3001 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca
     3061 ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     3121 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     3181 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     3241 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg
     3301 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     3361 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     3421 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     3481 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     3541 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     3601 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     3661 cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt
     3721 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     3781 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     3841 ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca ggtgcaaaga
     3901 tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa
     3961 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4021 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     4081 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4141 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4201 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     4261 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     4321 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     4381 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     4441 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     4501 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     4561 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     4621 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     4681 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     4741 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     4801 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     4861 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     4921 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     4981 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     5041 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     5101 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aggctagcac
     5161 cgccaccatg gaggttccag aacccacctg ccctgcccct cctgcgaggg accagccagc
     5221 tcccactcct ggccctccag gggccccagg tggccaggcc tcacctcacc tgaccctggg
     5281 ccctgtcctt ctgccgccag agcagggcct ggcccccccc actgtgttcc tgaaggccct
     5341 gcccatccca ctgtaccaca cggtgcctcc cgggggcctc cagccccgcg ccccgctagt
     5401 gacgggcagc ctagatgggg gcaacgtgcc cttcatactc agccctgtgc tgcagcctga
     5461 agggcctggc cccacccagg tggggaagcc ggcggcccct acgctgacgg tgaacatcgt
     5521 gggcactctg cctgtcctgt cgccgggcct gggccccacg ctgggcagcc caggcaaggt
     5581 gcggaatgcg ggcaagtacc tgtgtccgca ctgtggtcgc gactgcctga agcccagtgt
     5641 tctagagaag cacatccggt cccacacggg tgagaggccc ttcccgtgtg ccacctgcgg
     5701 catcgccttt aagacccaga gcaatctcta caagcacagg cggacgcaga cgcacctcaa
     5761 caactcccgg ctgtcctcag agtccgaggg cgccgggggc ggcctcctgg aggaagggga
     5821 caaggccgga gagcccccca gaccagaggg caggggcgag agcaggtgcc aggggatgca
     5881 cgaaggcgcc tcggagagac ccctttctcc gggtgcccac gtgcccctac ttgccaagaa
     5941 cctggatgtg aggaccgaag ctgctccctg tccagggtcc gcatttgccg acagagaggc
     6001 tccttgggac tctgccccca tggcgtcacc tgggctccca gcggccagca cacaaccctg
     6061 gcgtaagttg ccagagcaga agtcgccgac cgccgggaag ccgtgcgccc tgcagcggca
     6121 gcaggcgacg gcagcggaga agccctggga tgccaaggcc cccgagggcc ggctgcggaa
     6181 gtgtgagagc accgactcgg ggtacctgtc gcgctccgac agcgcggagc agccgcatgc
     6241 gccctgcagc cccctgcaca gcctttcgga gcacagcgcc gagtccgagg gggagggcgg
     6301 cccgggcccg gggccagggg tcgcaggggc cgagcccggg gcgcgagaag ccggcctgga
     6361 gctggagaag aagcggctgg aggagcgcat cgcccagctc atctcccaca accaggcggt
     6421 ggtggacgat gcccagctgg acaacgtgcg gccccggaag accgggctgt ccaaacaggg
     6481 cagcatcgac ctgcccacgc cctacaccta caaggactcc ttccactttg acatccgcgc
     6541 gctggagcca ggccgtagga gggccccggg ccccgtgcgc tccacctgga cgcccccaga
     6601 caagtctcgg cccctcttct tccactccgt ccccactcag ctctccacca ccgtggaatg
     6661 tgtccccgtc accaggagca actcgctgcc cttcgtcgag ggctccagga cgtggctgga
     6721 gcccagggag ccccgggacc cctggtccag gacgcagaag cctctgagcc ccaggcccgg
     6781 cccagcccgc ctgggctgcc gctcgggact aagctcgact gacgttccca gtgggcatcc
     6841 ccgggccctg gtcagacagg ccgcggtgga ggacctgcca ggcaccccca ttggcgatgc
     6901 cctggtgccc gcagaggaca cagacgcaaa gagaactgct gcgcgggagg ccatggccgg
     6961 caagggcaga gcgggcggca ggaagtgcgg ccagagaagg ctgaagatgt tctcccagga
     7021 gaagtggcag gtgtacgggg atgagacgtt caaaaggatc taccagaaaa tgaaagccag
     7081 tccccatgga ggcaagaaag ccagggaggt gggaatgggc agtggggcag aactgggctt
     7141 tcctctgcag aaagaggcag cagggagctc aggcacagtc cccacccaag acaggaggac
     7201 ccctgtccat gaggacatat ccgcaggggc aacgccagag ccttggggaa atccaccagc
     7261 cctggaggcc tccttggtga ctgaacccac taagcatggg gagacggtgg ccaggagagg
     7321 agacagtgac cgacccaggg tggaagaggc tgtgtcatcc cctgcactgg gtggcagaga
     7381 cagtccctgt tcaggcagta ggagccccct ggtctctcca aatgggaggc tggaactggg
     7441 gtggcagatg cccccagcac ctggccccct caaagggggt gatgtagagg ctcccaggcc
     7501 agtttggccg gaccccaagc tggaaggagg tgcccgaggt gtgggggatg ttcaggagac
     7561 ctgcctgtgg gcccagactg tcctgagatg gcccagcagg ggctcagggg aggacaagct
     7621 cccctcagag aggaagaagc tgaaagtgga ggacctgcac agctggaagc aaccagagcc
     7681 tgtgagcgca gagaccccag gtgggcccac gcagcctgcc tctttgtcat cccagaagca
     7741 ggatgccgat cccggggagg tgccaggggg ctcaaaggag agtgccaggc aggtgggcga
     7801 gcctctggag tcctctggag cctccttggc tgctgcttct gttgccctga agagggtggg
     7861 gccaagggac aaggctaccc cactgcatcc tgcagcccca gcccccgcag agcacccctc
     7921 gctggccacc ccacctcagg ctcctagagt gctctctgcc ctggcagata atgccttttc
     7981 ccccaagtac ctcctcaggt tacctcaggc agagaccccc ttaccactgc ccattccctg
     8041 gggaccaagg cacagccagg actctctctg cagcagtggg tggcctgaag aacgggcatc
     8101 atttgttggg tcaggactgg ggacccctct ttctcccagc ccagcctcag gcccctcccc
     8161 aggtgaggcg gacagcatcc tggaggaccc cagctgttcc aggccacagg atgggagaaa
     8221 aggggcacag ttgggggggg acaaggggga caggatggcc actagcaggc cagcagccag
     8281 ggagttgccc atctcagcac caggggctcc cagggaggct acctcctccc cgcccactcc
     8341 aacgtgtgag gcacacttag ttcaggacat ggagggtgac agccaccgta tccatcgcct
     8401 ctgcatgggc agcactttgg caagggccag gctctctggg gatgtcctga atccctgggt
     8461 acccaactgg gagctggggg agcctcctgg gaatgcccca gaagatcctt cttcagggcc
     8521 cctggtgggc cccgacccgt gttcccccct ccagcctggc tccttcctca ctgccctcac
     8581 tcggcctcag ggtgtgcccc caggctggcc agagctggcc ttgtcttccc actcagggac
     8641 gtcccggagc cacagcaccc gcagtcccca cagcacccaa aacccctttc cctcactgaa
     8701 ggctgagccg cggctcacgt ggtgttgcct gagccgcagt gtccctctgc ccgcggagca
     8761 gaaggcaaag gcggcatctg tgtacttggc ggtgcacttt cctggtagca gcctccgaga
     8821 tgagggtccc aatggccctc ctgggagcaa tggaggatgg acctggacaa gccctggaga
     8881 aggagggccg gcgcagatgt ccaagttctc ctacccaaca gtcccagggg tgatgcccca
     8941 gcaccaggtg tctgagccag aatggaagaa aggcctgcct tggagggcaa agatgtctcg
     9001 tgggaacagc aagcagagaa aactgaagat caaccctaaa aggtacaaag ggaatttctt
     9061 gcagagctgt gttcagctga gagccagtag acttcgcaca ccaacctggg tgcgaagaag
     9121 aagccgccac cctcccgcac ttgagggact gaagccatgc aggacccctg ggcagacctc
     9181 ttcagaaata gcaggtctga atctgcaaga ggagccatct tgtgccacct cagaatcacc
     9241 tccttgttgt gggaaggaag agaagaagga aggtgactgc agacaaacct taggaaccct
     9301 ctctcttggt acaagttcaa gaattgtcag ggaaatggac aaacgaactg tgaaggatat
     9361 ttctccatct gctggtgagc atggtgactg tactactcac agcactgctg ccacatcagg
     9421 attatctctg caatctgaca cctgcctggc agtggttaat gacgtgcctc taccccctgg
     9481 caaaggtctt gaccttgggt tgctggagac tcagctgctg gcctcccagg attcagtctc
     9541 aacagatccc aaaccataca tcttctcaga tgctcaaagg ccttcttcct ttgggtccaa
     9601 aggaactttt ccccaccatg acattgctac ctctgtggct gccgtttgta tttctctgcc
     9661 agtgagaaca gatcacatag cccaggaaat tcacagtgct gaatcacgag accacagcca
     9721 gactgcaggg aggactctga catcaagctc cccagacagc aaagtcacag aagagggcag
     9781 agcacagacc ctcttgccag ggagaccttc atctggacaa agaatttcag attcggttcc
     9841 actggagtca actgaaaaaa ctcatcttga aataccagct tcaggaccaa gttcagctag
     9901 ttcacaccac aaggaaggga gacacaagac gttttttcct tccagaggcc agtatgggtg
     9961 tggggaaatg actgtcccct gcccctcttt aggaagtgac ggtaggaaac gtcaggtatc
    10021 tggattaatc actcggaaag attctgtggt tccttctaag ccagagcagc ccatagaaat
    10081 tcctgaagcc ccttctaaat ccctcaagaa gaggagtctg gaaggaatga gaaagcaaac
    10141 tcgagtagag ttcagtgaca ccagcagcga cgatgaagac cgattagtta tagaaatata
    10201 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
    10261 aaaggacgac acccatatcc ctacgatagc agcacgcgtt aagtcgacaa tcaacctctg
    10321 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
    10381 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
    10441 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
    10501 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
    10561 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
    10621 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
    10681 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
    10741 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
    10801 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
    10861 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
    10921 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
    10981 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
    11041 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
    11101 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
    11161 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
    11221 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
    11281 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    11341 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    11401 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
    11461 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    11521 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    11581 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
    11641 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
    11701 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
    11761 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
    11821 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
    11881 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    11941 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
    12001 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    12061 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    12121 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
    12181 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
    12241 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    12301 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    12361 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    12421 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    12481 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    12541 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    12601 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    12661 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    12721 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    12781 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    12841 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    12901 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    12961 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    13021 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    13081 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    13141 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    13201 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    13261 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    13321 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    13381 aaaagatcaa aggatcttc
//
