LOCUS       sequence-489497-       17760 bp    DNA     circular SYN 16-OCT-2025
DEFINITION  Multigene vector for constitutive expression of mNeonGreen, VEGFR2 
            NTEVp chain (75S NTEVp mutant), and VEGFR1 CTEVp chain with ZF6a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17760)
  AUTHORS   Edelstein HI, Cosio A, Ezekiel ML, Corcoran WK, Morris AH, Leonard 
            JN
  TITLE     Conversion of natural cytokine receptors into orthogonal synthetic 
            biosensors.
  JOURNAL   Nat Chem Biol. 2025 Aug 22:10.1038/s41589-025-01986-1. doi: 
            10.1038/s41589-025-01986-1.
  PUBMED    40846994
REFERENCE   2  (bases 1 to 17760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Aug 22:10.1038/s41589-025-01986-1. doi: 
            10.1038/s41589-025-01986-1."
COMMENT     Sequence Label: L4860 VEGFR2 NTEVp chain (75S NTEVp mutant), V...
FEATURES             Location/Qualifiers
     source          1..17760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        477..856
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        857..1060
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1011..1031
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1105..1124
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1105..1123
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1143..1152
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1404..1413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1910..2134
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        3183..4365
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3414..4356
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             4535..4600
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    7324..7548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8597..9779
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8828..9770
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      9886..9895
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9970..10035
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             12685..12705
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             12724..12744
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12751..12900
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     polyA_signal    13281..13505
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    14294..14518
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      14564..14992
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14651..14670)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14861..14882
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        15015..15211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      15062..15197
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     15124..15143
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    15331..15452
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15368..15387)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15422..15441
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(15501..15517)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15501..15517)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15514..15536)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15525..15541
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15549..15555,15556..15573,15574..15579))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15594..15615
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15732..15749)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15903..16491)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15983..16002)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(16699..16793)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(16824..17618)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(16935..16954)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     17545..17564
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
       61 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
      121 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
      181 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgccaaggt cttgacaaac
      241 gagtgctagt acaagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
      301 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      361 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      421 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      481 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      541 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      601 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      661 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      721 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      781 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      841 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      901 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      961 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     1021 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
     1081 cactgcttac tggcttatcg aaattaatac gactcactat agggagtccc aagctggcta
     1141 gcgccaccat ggtttccaag ggcgaagagg acaatatggc cagcctgcct gccacacacg
     1201 agctgcacat ctttggcagc atcaacggcg tggacttcga catggtcgga caaggcaccg
     1261 gcaatcccaa cgacggctac gaggaactga acctgaagtc cacaaagggc gacctccagt
     1321 tcagcccctg gattctggtg cctcacatcg gctacggctt ccaccagtac ctgccttatc
     1381 ctgacggcat gagccctttc caggccgcca tggttgatgg ctctggctac caggtccacc
     1441 ggaccatgca gtttgaggat ggcgcttccc tgaccgtgaa ctacagatac acctacgagg
     1501 gcagccacat caaaggcgag gcccaagtga aaggcacagg cttccctgct gatggccccg
     1561 tgatgaccaa ttctctgaca gccgccgact ggtgcagaag caagaaaaca taccccaacg
     1621 acaagaccat catcagcacc ttcaagtggt cctacaccac cggcaacggc aagcggtaca
     1681 gaagcaccgc cagaaccacc tacaccttcg ccaaacctat ggccgccaac tacctgaaga
     1741 accagcctat gtacgtgttc cgcaagaccg agctgaagca ctccaagaca gaactgaact
     1801 tcaaagagtg gcagaaagcc ttcaccgacg tgatgggcat ggacgaactc tacaaatgag
     1861 cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     1921 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     1981 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     2041 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagtcaat
     2101 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcaggtcg agggtacatg
     2161 cgccttactc cttgtgagcg catcgcgata agctctagag ggacagcccc cccccaaagc
     2221 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
     2281 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
     2341 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
     2401 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
     2461 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
     2521 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
     2581 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
     2641 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
     2701 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
     2761 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
     2821 gcgcgcctgg tggcgaacga tcaacagacg ggagagggca aaaccgatgt tgacggacta
     2881 atcctgacaa gatctgggga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     2941 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     3001 cttcgcgatg tacgggccag atatacgcgt gagtaattca tacaaaagga ctcgcccctg
     3061 ccttggggaa tcccagggac cgtcgttaaa ctcccactaa cgtagaaccc agagatcgct
     3121 gcgttcccgc cccctcaccc gcccgctctc gtcatcactg aggtggagaa gagcatgcgt
     3181 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     3241 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     3301 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     3361 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     3421 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     3481 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     3541 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     3601 gcctggcttg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     3661 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     3721 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     3781 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     3841 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     3901 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3961 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     4021 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     4081 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     4141 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     4201 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     4261 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     4321 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgagagct cgtttagtga
     4381 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agagaacggg
     4441 accgatccaa attctcgagg ctagctgtac agccaccatg cagagcaagg tgctgctggc
     4501 tgttgccctg tggctgtgcg tggaaaccag agccgactac aaggaccacg acggcgatta
     4561 taaggatcac gacatcgatt acaaagacga cgacgacaag gccagcgtgg gcctgccttc
     4621 tgtttctctg gatctgccca gactgagcat ccagaaggac atcctgacca tcaaggccaa
     4681 caccacactc cagatcacct gtcggggaca gcgcgatctg gattggctgt ggcctaacaa
     4741 tcagagcggc agcgagcagc gcgtggaagt gacagagtgt tccgatggcc tgttctgcaa
     4801 gaccctgaca atccccaaag tgatcggcaa cgacaccggg gcctacaagt gcttctacag
     4861 agagacagac ctggcctccg tgatctacgt gtacgtgcag gactacagaa gcccctttat
     4921 cgccagcgtg tccgatcagc acggcgtggt gtatatcacc gagaacaaga acaagaccgt
     4981 ggtcatcccc tgcctgggca gcatcagcaa cctgaatgtg tccctgtgcg cccggtatcc
     5041 tgagaagaga ttcgtgcccg acggcaacag aatcagctgg gacagcaaga agggcttcac
     5101 tatccccagc tacatgatca gctacgccgg catggtgttc tgcgaggcca agatcaacga
     5161 cgagagctac cagagcatca tgtatatcgt ggtggtcgtg ggctaccgca tctacgatgt
     5221 ggtgctgagc cctagccacg gcatcgaact gtctgtggga gagaagctgg tgctgaactg
     5281 caccgccaga accgagctga atgtgggcat cgacttcaac tgggagtacc ccagcagcaa
     5341 gcaccagcac aagaaactgg tcaaccggga cctgaaaacc cagagcggct ccgagatgaa
     5401 gaagttcctg agcacactga caatcgacgg cgtgaccaga agcgaccagg gcctgtatac
     5461 ttgtgccgcc tctagcggcc tgatgaccaa gaaaaacagc accttcgtgc gggtccacga
     5521 gaagcctttt gtggcctttg gcagcggcat ggaaagcctg gtggaagcca cagtgggcga
     5581 gagagtcaga atccccgcta agtacctggg ctaccctcca cctgagatca agtggtacaa
     5641 gaacggcatc cctctggaaa gcaaccacac aatcaaggcc ggccacgtgc tgacaatcat
     5701 ggaagtgtcc gagagagaca ccggcaatta caccgtgatc ctgactaacc ccatcagcaa
     5761 agaaaagcag agccacgtgg tgtccctggt ggtgtatgtg cctcctcaga tcggcgagaa
     5821 gtccctgatc agccccgtgg acagctacca gtacggcacc acacagacac tgacctgcac
     5881 cgtgtacgcc attcctccac ctcaccacat ccactggtac tggcagctgg aagaggaatg
     5941 cgccaacgag ccttctcagg ccgtgtccgt gacaaacccc tatccttgtg aagagtggcg
     6001 gagcgtggaa gatttccaag gcggcaacaa gatcgaagtc aacaagaacc agttcgccct
     6061 gatcgagggc aagaacaaaa cagtgtctac cctcgtgatc caggccgcca atgtgtctgc
     6121 cctgtataag tgcgaggccg tgaacaaagt cggcagaggc gaaagagtga tcagcttcca
     6181 cgtgacaaga ggccccgaga tcaccctcca gccagatatg cagcctaccg agcaagagtc
     6241 cgtcagcctg tggtgtaccg ccgacagatc caccttcgag aacctgacct ggtacaagct
     6301 gggaccccag cctctgccta ttcacgtggg agaactgccc acacctgtgt gcaagaacct
     6361 ggacaccctg tggaaactga acgccaccat gttcagcaac agcaccaacg acattctgat
     6421 catggaactg aagaacgcca gcctccagga ccagggcgat tatgtgtgtc tggcccagga
     6481 cagaaagacc aaaaagcggc actgtgtcgt gcggcagctg accgtgcttg aaagagtggc
     6541 cccaaccatc accggcaacc tggaaaatca gaccaccagc atcggcgagt ccatcgaggt
     6601 gtcatgtacc gcctctggca acccacctcc acagatcatg tggttcaagg acaacgagac
     6661 actggtcgag gacagcggca tcgtgctgaa ggacggcaat cggaatctga ccattcggag
     6721 agtgcggaaa gaggacgagg gactgtatac ctgccaggcc tgtagcgtgc tgggctgtgc
     6781 taaagtggaa gccttcttta tcatcgaggg cgctcaagaa aagaccaacc tcgaaatcat
     6841 catcctcgtg ggcacagccg tgatcgccat gttcttttgg ctgctgctcg tgatcatcct
     6901 gcggaccgtg aaaggcgccg agagcttgtt taaggggccg cgtgattaca acccgatatc
     6961 gagcaccatt tgtcatttga cgaatgaatc tgatgggcac acaacatcgt tgtatggtat
     7021 tggatttggt cccttcatca ttacaaacaa gcacttgttt agaagaaata atggaacact
     7081 gttggtccaa tcactacatg gtgtattcaa ggtcaagaac accacgactt tgcaacaaag
     7141 cctcattgat gggagggaca tgataattat tcgcatgcct aaggatttcc caccatttcc
     7201 tcaaaagctg aaatttagag agccacaaag ggaagagcgc atatgtcttg tgacaaccaa
     7261 cttccaaact taggcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct
     7321 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     7381 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     7441 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     7501 attgggaagt caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcag
     7561 gtcgaagaac tatttcctgg ctgttacgcg agcgcatcgc gataagctct agagggacag
     7621 ccccccccca aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg
     7681 agccgcccgg ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg
     7741 cggggacagc ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg
     7801 ctgctctttg agcctgcaga cacctggggg gatacgggga aaaagcttta ggctgaaaga
     7861 gagatttaga atgacagaat catagaacgg cctgcgtacg gatggggctc tagctctaga
     7921 gcttgatatc gaattcctgc agccccgcta gagggacagc ccccccccaa agcccccagg
     7981 gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc
     8041 ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg
     8101 gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcagac
     8161 acctgggggg atacggggaa aaagctttag gctgaaagag agatttagaa tgacagaatc
     8221 atagaacggc ctgggcgcgc ctggtggcga acgatcaaca gacgggagag gactaaatag
     8281 tagttcagac gccgttaagc gcaagatctg gggaggtcgc tgagtagtgc gcgagcaaaa
     8341 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     8401 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtgagtaa ttcatacaaa
     8461 aggactcgcc cctgccttgg ggaatcccag ggaccgtcgt taaactccca ctaacgtaga
     8521 acccagagat cgctgcgttc ccgccccctc acccgcccgc tctcgtcatc actgaggtgg
     8581 agaagagcat gcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     8641 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
     8701 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     8761 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     8821 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     8881 ttgcgtgcct tgaattactt ccacgcccct ggctgcagta cgtgattctt gatcccgagc
     8941 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     9001 gtgcttgagt tgaggcctgg cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     9061 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     9121 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     9181 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     9241 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     9301 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     9361 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     9421 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     9481 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     9541 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     9601 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     9661 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     9721 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
     9781 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     9841 tagaagagaa cgggaccgat ccaaattctc gaggctagct gtacagccac catggtgtcc
     9901 tactgggata ccggcgttct gctgtgtgcc ctgctgtctt gtctgctgct gaccggatct
     9961 agcagcggcg actacaagga ccacgacggc gattataagg atcatgacat cgattacaag
    10021 gacgatgacg acaagagcaa gctgaaggac cccgagctga gcctgaaggg aacccagcat
    10081 attatgcagg ccggccagac actgcacctc cagtgtagag gcgaagccgc tcacaaatgg
    10141 tccctgcctg agatggtgtc caaagagagc gagcggctga gcatcaccaa gagcgcctgt
    10201 ggcagaaacg gcaagcagtt ctgctccaca ctgaccctga atactgccca ggccaaccac
    10261 accggcttct acagctgcaa gtatctggcc gtgcctacca gcaagaagaa agaaaccgag
    10321 agcgccatct acatcttcat cagcgacacc ggcagaccct tcgtggaaat gtactctgag
    10381 atccccgaga tcatccacat gaccgagggc agagagctgg tcatcccttg cagagtgaca
    10441 agccccaaca tcaccgtgac tctgaagaag ttccctctgg acacactgat ccccgacggc
    10501 aagagaatca tctgggacag ccggaagggc ttcatcatca gcaacgccac ctacaaagag
    10561 atcggcctgc tgacctgtga agccaccgtg aacggccacc tgtataagac caactacctg
    10621 acacacaggc agaccaacac catcatcgac gtgcagatca gcacccctcg gccagtgaaa
    10681 ctgctgagag gacacaccct ggtgctgaat tgcaccgcca ccacacctct gaacaccaga
    10741 gtgcagatga cctggtctta ccccgacgag aagaacaagc gggcttccgt gcggagaaga
    10801 atcgaccaga gcaacagcca cgccaacatc ttctactccg tgctgaccat cgacaagatg
    10861 cagaacaagg acaagggcct gtatacctgt agagtgcgga gcggccctag cttcaagagc
    10921 gtgaacacct ccgtgcatat ctacgacaag gccttcatca cagtgaagca ccggaagcag
    10981 caggtcctgg aaacagtggc cggcaagagg tcctacagac tgagcatgaa agtgaaggct
    11041 ttcccatctc ctgaggtcgt gtggctgaag gatggactgc ctgccacaga gaagtctgcc
    11101 agatacctga ccagaggcta cagcctgatc atcaaggacg tgaccgagga agatgccggc
    11161 aactacacca tcctgctgtc catcaagcag agcaacgtgt tcaagaacct gaccgccact
    11221 ctgatcgtga acgtgaagcc ccaaatctat gagaaggccg tgtccagctt tcccgatcct
    11281 gctctgtatc ccctgggctc cagacagatc ctgacctgta ccgcctatgg catccctcag
    11341 cctaccatca agtggttctg gcacccctgc aaccacaacc acagcgaggc cagatgcgac
    11401 ttctgtagca acaacgagga atccttcatc ctggacgccg acagcaacat gggcaacaga
    11461 atcgagagca tcacacagcg catggccatc attgagggca agaacaagat ggcctcaact
    11521 ctggtggtgg ccgactccag aatcagcggc atctatatct gtatcgccag caacaaagtg
    11581 ggcaccgtgg gcagaaacat cagcttctac atcaccgatg tgcccaacgg cttccacgtg
    11641 aacctggaaa agatgcccac cgaaggcgag gacctgaagc tgagctgtac cgtgaacaag
    11701 ttcctgtacc gggatgtgac ctggattctg ctgcggacag tgaacaaccg gaccatgcac
    11761 tacagcatca gcaagcagaa gatggccatt accaaagagc acagcatcac cctgaatctg
    11821 accatcatga acgtgtccct gcaagacagc ggcacctacg cctgtagagc ccggaatgtg
    11881 tataccggcg aggaaatcct gcaaaagaaa gagatcacca tccgggacca agaggcccct
    11941 tacctgctga gaaacctgag cgatcacacc gtggccatca gctccagcac cacactggac
    12001 tgtcatgcca atggcgtgcc cgagcctcag atcacctggt ttaagaacaa ccacaagatc
    12061 cagcaagagc ccggcatcat tctcggccct ggaagctcta ccctgttcat cgagagagtg
    12121 accgaagagg acgagggcgt gtaccactgc aaggccacca atcagaaagg cagcgtggaa
    12181 agcagcgcct acctgaccgt tcagggcacc agcgacaagt ccaacctgga actgatcact
    12241 ctgacctgca cctgtgtggc cgctacactg ttttggctgc tgctcaccct gtttattcgg
    12301 aagatgaagc ggggcgccaa gagcatgtct agcatggtgt cagacactag ttgcacattc
    12361 ccttcatctg atggcatatt ctggaagcat tggattcaaa ccaaggatgg gcagtgtggc
    12421 agtccattag tatcaactag agatgggttc attgttggta tacactcagc atcgaatttc
    12481 accaacacaa acaattattt cacaagcgtg ccgaaaaact tcatggaatt gaaaacaaat
    12541 caggaggcgc agcagtgggt tagtggttgg cgattaaatg ctgactcagt attgtggggg
    12601 ggccataaag ttttcatggt gaaacctgaa gagccttttc agccagttaa ggaagcgact
    12661 caactcatga atcttaaggg ctcggagaac ctgtattttc agatgggggg aggcagcggg
    12721 ggccctaaga aaaagcgcaa agtctccgga gacgctctgg atgactttga tctggacatg
    12781 cttggttccg atgccttgga cgattttgac ctcgatatgc tgggctccga cgctctcgac
    12841 gatttcgacc tcgacatgct gggtagcgat gcactggatg actttgatct ggatatgctc
    12901 ggatccggtg gtggcgggtc tggaggagga ggtagtggtg gaggtggctc tggtaccgct
    12961 agacccggcg aaagaccttt ccagtgccgg atctgcatga ggaacttcag caagggcgag
    13021 agactcgtgc ggcacaccag aacacacaca ggcgagaagc ccttccagtg tagaatctgt
    13081 atgcgcaact tcagccggat ggacaacctg agcacccacc tgagaaccca taccggggag
    13141 aagccatttc aatgccgcat ctgtatgaga aatttttccc ggaaggacgc cctgaaccgg
    13201 cacctgaaaa cacacctgag aggcagctga gcggccgctc gagtctagag ggcccgttta
    13261 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
    13321 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
    13381 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
    13441 ggacagcaag ggggaggatt gggaagtcaa tagcaggcat gctggggatg cggtgggctc
    13501 tatggcttct gaggcaggtc gaagagactg cccgacacat cttagtgagc gcatcgcgat
    13561 aagctctaga gggacagccc ccccccaaag cccccaggga tgtaattacg tccctccccc
    13621 gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc cccccgcatc
    13681 cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt
    13741 cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctggggggat acggggaaaa
    13801 agctttaggc tgaaagagag atttagaatg acagaatcat agaacggcct gcgtacggat
    13861 ggggctctag ctctagagct tgatatcgaa ttcctgcagc cccgctagag ggacagcccc
    13921 cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc
    13981 gcccggggct ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg
    14041 gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc
    14101 tctttgagcc tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga
    14161 tttagaatga cagaatcata gaacggcctg ggcgcgcctg gtggcgaacg atcaacagac
    14221 gggagaggtt acgaggatgc acatgtgacc gagggatcta gagggcccgt ttaaacccgc
    14281 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
    14341 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
    14401 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
    14461 aagggggagg attgggaagt caatagcagg catgctgggg atgcggtggg ctctatggct
    14521 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
    14581 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
    14641 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
    14701 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    14761 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    14821 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    14881 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    14941 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
    15001 ggaatgtgtg tcagttaggc tcaattagtc agcaaccata gtcccgcccc taactccgcc
    15061 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    15121 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
    15181 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
    15241 cggaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
    15301 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
    15361 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    15421 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
    15481 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    15541 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    15601 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    15661 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    15721 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    15781 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    15841 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    15901 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    15961 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    16021 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    16081 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    16141 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    16201 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    16261 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    16321 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    16381 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    16441 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    16501 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    16561 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    16621 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttgacattg
    16681 gtcatggcta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
    16741 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcca
    16801 attctcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
    16861 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
    16921 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
    16981 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
    17041 aagcaggcat cgccgtgggt cacgacgaga tcctcgccgt cgggcatacg cgccttgagc
    17101 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
    17161 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
    17221 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
    17281 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
    17341 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagccgcgca aggaacgccc
    17401 gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac
    17461 aggtcggtct tgacaaaaag aaccgggcga ccctgcgctg acagccggaa cacggcggca
    17521 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcc
    17581 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
    17641 tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag
    17701 tttactttgc agggcttccc aaccttacca gagggcggcc caactggcaa ttccgacgtc
//
