LOCUS       sequence-489509-        8691 bp    DNA     circular SYN 16-OCT-2025
DEFINITION  DOX-regulated lentiviral expression of 99xGly-EGFP-3xHA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Yang J, Xu Y, Ziehr DR, Taylor MS, Valenstein ML, Frenkel EM, Bush 
            JR, Rutter K, Stevanovski I, Shi CY, Kesavan M, Pinto RM, Deveson I,
            Bartel DP, Sabatini DM, Chivukula RR
  TITLE     Polyglycine-mediated aggregation of FAM98B disrupts tRNA processing 
            in GGC repeat disorders.
  JOURNAL   Science. 2025 Jul 17;389(6757):eado2403. doi: 
            10.1126/science.ado2403. Epub 2025 Jul 17.
  PUBMED    40674500
REFERENCE   2  (bases 1 to 8691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-07-17"; volume: "389(6757):eado2403. doi"; pages: " 
            10.1126/science.ado2403. Epub 2025 Jul 17"
COMMENT     Sequence Label: pDOXOFF 99xGly
FEATURES             Location/Qualifiers
     source          1..8691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      40..175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        175..246
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(247..315,316..1107)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(465..484)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(1263..1851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1343..1362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2020..2037
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2154..2175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2190..2195,2196..2213,2214..2220)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2228..2244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2233..2255
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2252..2268
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2252..2268
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2287..2307
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2289..2307
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2335..2561
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2562..2742
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2789..2914
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3407..3640
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3825..3869
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4082..4199
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4268..4646
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    4276..4294
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4312..4330
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4348..4366
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4384..4402
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4420..4438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4456..4474
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4492..4510
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4555..4579
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4690..4699
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5026..5028,5029..5739)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5026..5028,5029..5739)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5068..5089)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5329..5348)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5676..5697
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5752..5778
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5791..5817
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5830..5856
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5869..6379
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6298..6316
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6389..6781
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             6791..6844
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6845..7462,7463..7468,7469..7504,7505..7507,7508..7543,
                     7544..7546,7547..7582,7583..7591)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7631..7651)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8049..8060)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8265..8498
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8570..8691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8607..8626)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8661..8680
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
       61 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
      121 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctttca
      181 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      241 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      301 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      361 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      421 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      481 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      541 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
      601 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
      661 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
      721 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
      781 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
      841 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
      901 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
      961 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1021 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     1081 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1141 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1201 cacgcgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1261 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1321 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1381 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1441 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1501 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1561 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1621 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1681 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     1741 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1801 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1861 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1921 gacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     1981 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2041 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     2101 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     2161 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     2221 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     2281 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     2341 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     2401 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     2461 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     2521 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     2581 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     2641 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     2701 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     2761 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     2821 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     2881 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     2941 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     3001 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     3061 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     3121 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     3181 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     3241 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     3301 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     3361 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     3421 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     3481 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     3541 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     3601 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     3661 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     3721 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     3781 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     3841 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     3901 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     3961 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     4021 acctcccaac cccgagggga caattctcga cctcgagaca aatggcagta ttcatccaca
     4081 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     4141 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     4201 gggtttatta cagggacagc agagatccac tttggccgcg aatcgggatt cgcccttaag
     4261 ctagctcgag tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca
     4321 gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt
     4381 tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac
     4441 gtatctacag tttactccct atcagtgata gagaacgtat atccagttta ctccctatca
     4501 gtgatagaga acgtataagc tttaggcgtg tacggtgggc gcctataaaa gcagagctcg
     4561 tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt
     4621 tccgtaccac ttcctaccct cgtaaagtca gttggccagg cctagttggc caggcctagt
     4681 gccgaattcg ccaccatggg gttgccgctg ccagggggcg tgcggcagcg cggcggcggc
     4741 ggcggcggcg gcggcggcgg cggcggcggc ggaggcggcg gcggcggcgg cggcggcggc
     4801 ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc
     4861 ggcggcggcg gcggcggcgg cggcggcggc ggcggtggcg gcggcggcgg cggcggcggc
     4921 ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc
     4981 ggcggcggcg gcggcggcgg cggcggcggc ggctgggcct cgacggtgag caagggcgag
     5041 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     5101 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     5161 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     5221 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     5281 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     5341 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     5401 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     5461 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     5521 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     5581 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     5641 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     5701 gccgccggga tcactctcgg catggacgag ctgtacaagg gagggagcgg ctatccctat
     5761 gacgtgcctg attacgccgg cacaggatca tacccctatg atgtgcctga ctacgctggc
     5821 agcgccggat acccttatga tgtgcctgat tatgcttagt gaggatccgg ggttggggtt
     5881 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
     5941 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
     6001 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
     6061 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
     6121 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
     6181 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
     6241 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     6301 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     6361 caccgacctc tctccccagc aattcaccat ggttcctttg tctcaagaag aatccaccct
     6421 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
     6481 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
     6541 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
     6601 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
     6661 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
     6721 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
     6781 ccccggtgcc gaaggtagag gttctctcct cacttgtggt gatgttgaag aaaaccctgg
     6841 tccaatgtct agactggaca agagcaaagt cataaactct gctctggaat tactcaatga
     6901 agtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     6961 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgg caatcgagat
     7021 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
     7081 gcggaacaac gccaagtcat tccgctgtgc tctcctctca catcgcgacg gggctaaagt
     7141 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     7201 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     7261 ctttacactg ggctgcgtat tggaggatca ggagcatcaa gtagcaaaag aggaaagaga
     7321 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgacca
     7381 tcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     7441 acagctaaag tgcgaaagcg gcgggccggc cgacgccctt gacgattttg acttagacat
     7501 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     7561 cgattttgac cttgacatgc tccccgggta actaagtaag gatcgatcca agatatcgta
     7621 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7681 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7741 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     7801 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     7861 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     7921 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     7981 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     8041 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     8101 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     8161 ccccgcctgt ttcgcctcgg cgtccggact agaggtacct ttaagaccaa tgacttacaa
     8221 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     8281 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8341 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8401 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8461 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     8521 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     8581 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8641 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt a
//
