LOCUS       sequence-489510-        8690 bp    DNA     circular SYN 16-OCT-2025
DEFINITION  DOX-regulated lentiviral expression of +1 frameshifted 
            99xGly-EGFP-3xHA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Yang J, Xu Y, Ziehr DR, Taylor MS, Valenstein ML, Frenkel EM, Bush 
            JR, Rutter K, Stevanovski I, Shi CY, Kesavan M, Pinto RM, Deveson I,
            Bartel DP, Sabatini DM, Chivukula RR
  TITLE     Polyglycine-mediated aggregation of FAM98B disrupts tRNA processing 
            in GGC repeat disorders.
  JOURNAL   Science. 2025 Jul 17;389(6757):eado2403. doi: 
            10.1126/science.ado2403. Epub 2025 Jul 17.
  PUBMED    40674500
REFERENCE   2  (bases 1 to 8690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-07-17"; volume: "389(6757):eado2403. doi"; pages: " 
            10.1126/science.ado2403. Epub 2025 Jul 17"
COMMENT     Sequence Label: pDOXOFF (+1)99xGly
FEATURES             Location/Qualifiers
     source          1..8690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      40..175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        175..246
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(247..315,316..1107)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(465..484)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(1262..1850)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1342..1361)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2019..2036
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2153..2174
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2189..2194,2195..2212,2213..2219)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2227..2243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2232..2254
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2251..2267
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2251..2267
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2286..2306
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2288..2306
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2334..2560
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2561..2741
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2788..2913
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3406..3639
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3824..3868
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4081..4198
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4267..4645
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    4275..4293
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4311..4329
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4347..4365
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4383..4401
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4419..4437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4455..4473
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4491..4509
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4554..4578
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4689..4698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5025..5027,5028..5738)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5025..5027,5028..5738)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5067..5088)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5328..5347)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5675..5696
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5751..5777
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5790..5816
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5829..5855
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5868..6378
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6297..6315
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6388..6780
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             6790..6843
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6844..7461,7462..7467,7468..7503,7504..7506,7507..7542,
                     7543..7545,7546..7581,7582..7590)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7630..7650)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8048..8059)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8264..8497
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8569..8690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8606..8625)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8660..8679
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
       61 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
      121 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctttca
      181 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      241 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      301 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      361 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      421 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      481 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      541 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
      601 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
      661 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
      721 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
      781 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
      841 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
      901 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
      961 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1021 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     1081 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1141 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgaaatc
     1201 acgcgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1261 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1321 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1381 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1441 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1501 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1561 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1621 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1681 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     1741 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1801 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1861 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1921 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1981 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     2041 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     2101 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2161 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2221 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2281 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     2341 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     2401 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     2461 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     2521 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     2581 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     2641 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     2701 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     2761 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     2821 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     2881 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     2941 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     3001 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     3061 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     3121 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     3181 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     3241 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     3301 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     3361 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     3421 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     3481 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     3541 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     3601 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     3661 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     3721 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     3781 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     3841 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     3901 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     3961 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     4021 cctcccaacc ccgaggggac aattctcgac ctcgagacaa atggcagtat tcatccacaa
     4081 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4141 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     4201 ggtttattac agggacagca gagatccact ttggccgcga atcgggattc gcccttaagc
     4261 tagctcgagt ttactcccta tcagtgatag agaacgtatg aagagtttac tccctatcag
     4321 tgatagagaa cgtatgcaga ctttactccc tatcagtgat agagaacgta taaggagttt
     4381 actccctatc agtgatagag aacgtatgac cagtttactc cctatcagtg atagagaacg
     4441 tatctacagt ttactcccta tcagtgatag agaacgtata tccagtttac tccctatcag
     4501 tgatagagaa cgtataagct ttaggcgtgt acggtgggcg cctataaaag cagagctcgt
     4561 ttagtgaacc gtcagatcgc ctggagcaat tccacaacac ttttgtctta taccaacttt
     4621 ccgtaccact tcctaccctc gtaaagtcag ttggccaggc ctagttggcc aggcctagtg
     4681 ccgaattcgc caccatgggt tgccgctgcc agggggcgtg cggcagcgcg gcggcggcgg
     4741 cggcggcggc ggcggcggcg gcggcggcgg aggcggcggc ggcggcggcg gcggcggcgg
     4801 cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg
     4861 cggcggcggc ggcggcggcg gcggcggcgg cggtggcggc ggcggcggcg gcggcggcgg
     4921 cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg
     4981 cggcggcggc ggcggcggcg gcggcggcgg ctgggcctcg actggtgagc aagggcgagg
     5041 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     5101 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     5161 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     5221 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     5281 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     5341 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     5401 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     5461 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     5521 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     5581 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     5641 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     5701 ccgccgggat cactctcggc atggacgagc tgtacaaggg agggagcggc tatccctatg
     5761 acgtgcctga ttacgccggc acaggatcat acccctatga tgtgcctgac tacgctggca
     5821 gcgccggata cccttatgat gtgcctgatt atgcttagtg aggatccggg gttggggttg
     5881 cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc
     5941 gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc
     6001 acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta
     6061 agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt
     6121 ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga
     6181 tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg
     6241 gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt
     6301 tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc
     6361 accgacctct ctccccagca attcaccatg gttcctttgt ctcaagaaga atccaccctc
     6421 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc
     6481 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact
     6541 gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac
     6601 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg
     6661 tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat
     6721 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc
     6781 cccggtgccg aaggtagagg ttctctcctc acttgtggtg atgttgaaga aaaccctggt
     6841 ccaatgtcta gactggacaa gagcaaagtc ataaactctg ctctggaatt actcaatgaa
     6901 gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct
     6961 accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctggc aatcgagatg
     7021 ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg
     7081 cggaacaacg ccaagtcatt ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg
     7141 catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc
     7201 ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac
     7261 tttacactgg gctgcgtatt ggaggatcag gagcatcaag tagcaaaaga ggaaagagag
     7321 acacctacca ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccat
     7381 cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa
     7441 cagctaaagt gcgaaagcgg cgggccggcc gacgcccttg acgattttga cttagacatg
     7501 ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac
     7561 gattttgacc ttgacatgct ccccgggtaa ctaagtaagg atcgatccaa gatatcgtat
     7621 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7681 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7741 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7801 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7861 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7921 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     7981 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     8041 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     8101 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     8161 cccgcctgtt tcgcctcggc gtccggacta gaggtacctt taagaccaat gacttacaag
     8221 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     8281 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     8341 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8401 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8461 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     8521 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     8581 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8641 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
//
