LOCUS       sequence-489528-        7383 bp    DNA     circular SYN 16-OCT-2025
DEFINITION  Expresses SARS-CoV-2 spike (BA.1) protein for creating pseudotyped 
            virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Youssef N, Gurev S, Ghantous F, Brock KP, Jaimes JA, Thadani NN, 
            Dauphin A, Sherman AC, Yurkovetskiy L, Soto D, Estanboulieh R, 
            Kotzen B, Notin P, Kollasch AW, Cohen AA, Dross SE, Erasmus J, 
            Fuller DH, Bjorkman PJ, Lemieux JE, Luban J, Seaman MS, Marks DS
  TITLE     Computationally designed proteins mimic antibody immune evasion in 
            viral evolution.
  JOURNAL   Immunity. 2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015.
  PUBMED    40345199
REFERENCE   2  (bases 1 to 7383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015."
COMMENT     Sequence Label: pDMJ2-SARS-CoV-2-Spike (BA.1)
FEATURES             Location/Qualifiers
     source          1..7383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    3779..4003
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4418..5006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4498..4517)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5177..5968,5969..6037))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5800..5819
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6038..6142)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6325..6704
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6705..6908
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6859..6879
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6905..6929
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgttcgtgt ttcttgttct gcttcccttg gtctctagtc agtgcgtcaa cctgaccacc
       61 cgaactcagt tgcccccagc gtataccaat tcctttacta gaggcgtgta ctatcccgac
      121 aaggtcttta gaagcagcgt tttgcacagc acccaggacc tctttctgcc cttcttctca
      181 aacgtaacat ggtttcatgt aatttcaggg accaatggga caaaaaggtt cgacaacccc
      241 gtattgccat ttaacgatgg cgtgtacttc gcaagtatcg aaaagtcaaa tatcattcga
      301 ggatggatct ttgggacgac tctggatagt aagactcaaa gcctgctgat cgtgaataat
      361 gccacaaatg tcgtcataaa ggtgtgcgag ttccagttct gcaacgatcc gttcctggat
      421 cataagaaca acaaatcttg gatggaaagt gagttccggg tgtactcctc cgccaacaat
      481 tgtacctttg agtatgtatc ccagccattc ctgatggacc tcgaagggaa acagggcaat
      541 tttaagaacc tgcgcgaatt cgtgttcaaa aacatcgacg gttattttaa gatctattcc
      601 aaacacaccc cgatcatcgt tagggagccc gaggatctgc ctcaaggctt tagtgctctg
      661 gaaccgttgg tcgacttgcc tattggcatt aacatcaccc gcttccaaac actgctcgca
      721 ctccatcggt cttacctgac gcctggagac tcatccagcg gatggactgc cggagcggct
      781 gcatactatg tcggatatct tcagcctagg actttccttc tcaagtacaa cgagaatggc
      841 acgattaccg acgctgttga ttgcgccctg gacccactgt ctgaaaccaa atgcacactc
      901 aaaagcttca cagtcgagaa gggcatatac cagacaagca acttcagagt tcagcccaca
      961 gaatcaattg ttcgcttccc caatatcacc aacttgtgcc cttttgacga ggtattcaat
     1021 gcaaccagat tcgcgagcgt gtacgcctgg aacaggaaaa gaataagcaa ttgtgttgct
     1081 gattattctg tgctgtacaa tctggccccc ttctttactt tcaaatgcta cggtgtctcc
     1141 ccaaccaagc tcaatgatct gtgcttcact aacgtgtatg cagatagctt tgtgatcagg
     1201 ggtgacgagg tccggcaaat tgcacctggc cagaccggaa acattgccga ctataattac
     1261 aaactgccag atgatttcac ggggtgcgtt attgcctgga actccaacaa gctcgatagc
     1321 aaagtgtcag gcaattacaa ctacctctac aggctctttc gaaaaagcaa cctgaaacct
     1381 tttgagcgcg atattagcac cgagatctac caggctggaa acaagccctg caatggagtc
     1441 gccggcttta attgttactt tcctttgcgc tcctacagct ttcggcctac atacggcgtt
     1501 ggtcatcagc catatcgcgt tgtagtgctg tcatttgagc ttcttcatgc ccctgctaca
     1561 gtgtgtggcc ccaagaaatc taccaacctg gtgaaaaata aatgcgtgaa tttcaatttc
     1621 aacggtctga aaggtacggg ggttcttaca gaatccaaca aaaaatttct tccattccag
     1681 caattcggga gagatatcgc cgacaccact gatgccgtgc gagatcccca gacattggag
     1741 attctggaca tcactccttg ttcttttggt ggagtgagtg ttattacgcc cggcacaaac
     1801 actagcaatc aagtggctgt cctctaccag ggcgtaaact gcaccgaggt ccccgttgct
     1861 atccacgccg accagctgac tccaacgtgg cgggtatact ctactgggag caacgttttc
     1921 cagaccaggg ccggctgtct catcggagcc gagtacgtga acaactctta tgaatgtgat
     1981 attccaatcg gagcagggat ttgcgcctct tatcagaccc agacaaagag tcatcgcagg
     2041 gctaggagtg tggcctccca gtccatcatc gcatatacaa tgtccctggg ggctgaaaat
     2101 agcgtggcct atagtaacaa ttctatcgct atccccacaa attttaccat ctctgtcact
     2161 acggagatac tgccagtctc tatgacaaaa accagcgtgg actgtaccat gtacatatgc
     2221 ggcgattcaa ctgaatgctc caacctgctt ctgcagtacg gttccttctg cacacaactg
     2281 aagcgagcgc tcacaggcat cgcagtcgaa caggataaaa acactcagga ggtgtttgcg
     2341 caggtgaaac agatttacaa gactccacca attaagtatt tcggtgggtt caatttctct
     2401 caaatactgc cagatccgtc taagcctagt aagcggagct ttatagagga tttgctcttt
     2461 aataaggtga ccctcgctga tgctggattt ataaagcagt atggagactg tctgggtgac
     2521 attgctgctc gggatctgat ttgtgcacag aagtttaagg gcctgactgt actgcctccg
     2581 ttgcttacgg atgagatgat agcgcaatac acctccgcgc tgttggccgg caccattact
     2641 tccggatgga cattcggcgc aggagctgca ctccaaattc cttttgcgat gcagatggca
     2701 tatcgcttca atggaattgg ggtgacccag aatgtcctgt acgaaaatca gaagctgatt
     2761 gcgaaccagt tcaattccgc cattgggaaa atccaggata gtctgagctc cacagcaagc
     2821 gcactcggga agctgcaaga cgtggtcaat cacaacgccc aggccttgaa caccctggtg
     2881 aagcaactca gttcaaagtt cggcgccata agctccgtgc tgaatgacat cttcagtaga
     2941 ctggataagg tggaggccga agtgcagatc gaccggctga ttactggaag actgcagtca
     3001 cttcagactt atgtcacaca gcagcttatc agagccgcag agattagggc atctgccaat
     3061 cttgctgcta caaaaatgtc agaatgcgtg ctggggcagt caaagcgggt cgacttttgt
     3121 ggtaagggct atcacctgat gagcttcccc caatccgccc cacacggcgt tgtgttcctc
     3181 cacgttacct atgtgcccgc tcaagagaag aactttacca ccgcccctgc catctgccat
     3241 gacgggaagg cacactttcc aagagaaggc gtgtttgtga gcaatggaac acactggttt
     3301 gtgacccaaa gaaacttcta tgagccccag attataacaa ctgacaacac attcgtatct
     3361 ggtaattgtg acgttgtcat tgggatcgtg aacaacacgg tatacgaccc tctgcaaccg
     3421 gaattggact ctttcaagga ggaactcgac aagtatttta agaatcacac ctcacccgat
     3481 gtggaccttg gcgacatcag tggaattaac gcttccgtcg tcaacatcca gaaagagatc
     3541 gacagactga acgaagtggc caagaatctg aacgagtcac tgattgacct gcaggagctg
     3601 ggaaaatacg agcagtacat caaatggcct tggtacatct ggctggggtt tatcgccggg
     3661 ctcatagcta tagtgatggt gaccatcatg ctgtgttgca tgactagctg ctgtagctgt
     3721 ctgaagggct gttgctcatg cggctcttgt tgtaaataat gatgaggatc cagatctgct
     3781 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     3841 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     3901 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     3961 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tgggtaccca ggtgctgaag
     4021 aattgacccg gttcctcctg ggccagaaag aagcaggcac atccccttct ctgtgacaca
     4081 ccctgtccac gcccctggtt cttagttcca gccccactca taggacactc atagctcagg
     4141 agggctccgc cttcaatccc acccgctaaa gtacttggag cggtctctcc ctccctcatc
     4201 agcccaccaa accaaaccta gcctccaaga gtgggaagaa attaaagcaa gataggctat
     4261 taagtgcaga gggagagaaa atgcctccaa catgtgagga agtaatgaga gaaatcatag
     4321 aattttaagg ccatgattta aggccaatcg gaaagaacat gtgagcaaaa ggccagcaaa
     4381 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4441 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4501 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4561 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4621 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4681 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4741 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4801 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4861 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4921 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4981 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5101 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5161 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5221 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5281 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5341 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5401 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5461 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5521 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5581 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5641 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5701 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5761 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5821 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5881 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5941 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6001 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6061 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6121 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcccaatga
     6181 ttgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcaga
     6241 ttggctattg gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct
     6301 catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa
     6361 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     6421 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     6481 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     6541 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     6601 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     6661 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     6721 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     6781 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     6841 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     6901 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     6961 tccatagaag acaccgggac cgatccagcc tccatcggct cgcatctctc cttcacgcgc
     7021 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     7081 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     7141 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     7201 cctgaccctg cttgctcaac tctagttaac ggtggagggc agtgtagtct gagcagtact
     7261 cgttgctgcc gcgcgcgcca ccagacataa tagctgacag actaacagac tgttcctttc
     7321 catgggtctt ttctgcagtc accgtcgtcg acacgtgtga tcagatatcg cggccgctct
     7381 aga
//
