LOCUS       sequence-489567-        9121 bp    DNA     circular SYN 16-OCT-2025
DEFINITION  Hair shaft (HS)-specific Cux1 epicenter-driven fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Adam RC, Yang H, Rockowitz S, Larsen SB, Nikolova M, Oristian DS, 
            Polak L, Kadaja M, Asare A, Zheng D, Fuchs E
  TITLE     Pioneer factors govern super-enhancer dynamics in stem cell 
            plasticity and lineage choice.
  JOURNAL   Nature. 2015 May 21;521(7552):366-70. doi: 10.1038/nature14289. Epub
            2015 Mar 18.
  PUBMED    25799994
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14289"; journalName: "Nature"; date: "2015-05-21- 
            21"; volume: "521"; issue: "7552"; pages: "366-70"
COMMENT     Sequence Label: Cux1-SE(HS)-eGFP-PGK-H2BRFP
FEATURES             Location/Qualifiers
     source          1..9121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             824..1057
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1129..1250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1166..1185)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1220..1239
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1290..1425
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1445..1464)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1446..1464)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1474..1491)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1474..1490)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1483..1505)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1632..2087
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1719..1738)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1929..1950
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2113..2217
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2218..2286,2287..3078)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2436..2455)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3249..3837
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3738..3757
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3991..4008
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4125..4146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4161..4166,4167..4184,4185..4191)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4199..4215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4204..4226
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4223..4239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4223..4239
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4258..4278
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4260..4278
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4306..4532
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4533..4713
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4760..4885
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5378..5611
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5796..5840
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6138..6386
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6138..6158
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6309..6328
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    6482..6599
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6648..7158
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7077..7095
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7170..7547
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      7560..7569
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7566..8243
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             7566..8243
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(7696..7714)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7964..7983
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8158..8181
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             complement(join(8288..9001,9002..9004,9005..9007))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8291..9001,9002..9004,9005..9007))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8333..8354)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8682..8701
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8941..8962
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      9004..9013
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(join(9035..9121,1..109))
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      join(9049..9121,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
       61 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgcc cgatgcacat
      121 caaaggcagc cagggccagg gcccacccac gactgcccac ctcgggcgct gccctccgcc
      181 gtcctgggag acctcgggct gggttgaagg cacagcctca gaagtggcct ccgcgggcgc
      241 caggtgcagc acggtggcgc tcattaggcc tcggaaggcg ggccaggctt agagcccagt
      301 cttctgagaa tccccggaga agaaaggaat caaaggctgg ggattgggaa acaaggatga
      361 ggagataatg aaacaagatt cctatctgaa ataatttcct ctaattgctg gtaagaaaca
      421 ttcattttcc ttttgaagct cccggggccc cggggcgtct tgggacgtgc caagatggca
      481 gctcgtctag ccggatcggc ttgagagctg cctgcggggg ctggcactgg gccacggcgg
      541 ggcaggcagc gggggtgatg ctccacttga tggggcgcct aaggtgtgca agaggccttg
      601 ggagcgcggg cctttgtacc aaactcctgg tttccctctc ctgtgtgaga tggacacacc
      661 actctgaccc cagcccctac tttgtgcacc cactgggtgc cctgctgggc gaggcctgat
      721 aacctgattt ttctgctgat cactacaggt gaggtacctt taagaccaat gacttacaag
      781 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
      841 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
      901 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      961 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     1021 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     1081 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     1141 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     1201 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     1261 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     1321 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     1381 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     1441 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     1501 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     1561 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     1621 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     1681 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     1741 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     1801 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     1861 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     1921 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     1981 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2041 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     2101 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2161 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2221 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2281 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2341 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2401 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2461 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2521 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2581 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2641 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2701 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2761 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2821 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2881 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2941 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3001 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3061 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3121 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3181 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3241 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3301 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3361 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3421 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3481 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3541 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3601 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3661 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3721 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3781 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3841 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3901 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3961 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4021 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     4081 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     4141 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     4201 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     4261 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     4321 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     4381 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     4441 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     4501 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
     4561 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4621 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4681 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     4741 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     4801 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     4861 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     4921 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     4981 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     5041 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     5101 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     5161 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     5221 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     5281 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     5341 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     5401 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     5461 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     5521 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     5581 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     5641 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     5701 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     5761 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     5821 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     5881 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     5941 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     6001 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     6061 acagatccat tcgattagtg aacggatctc gacggtatcg atcacgagac tagcctcgag
     6121 cggccgcccc cttcaccgag ggcctatttc ccatgattcc ttcatatttg catatacgat
     6181 acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag atattagtac
     6241 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     6301 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     6361 atatcttgtg gaaaggacga aacaccggca acaagatgaa gagcaccaac tcgagttggt
     6421 gctcttcatc ttgttgtttt taattctcga cctcgagaca aatggcagta ttcatccaca
     6481 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     6541 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     6601 gggtttatta cagggacagc agagatccac tttggccgcg gctcgagggg gttggggttg
     6661 cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc
     6721 gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc
     6781 acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta
     6841 agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt
     6901 ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga
     6961 tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg
     7021 gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt
     7081 tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc
     7141 accgacctct ctccccaggg ggatctacca tgccagagcc agcgaagtct gctcccgccc
     7201 cgaaaaaggg ctccaagaag gcggtgacta aggcgcagaa gaaaggcggc aagaagcgca
     7261 agcgcagccg caaggagagc tattccatct atgtgtacaa ggttctgaag caggtccacc
     7321 ctgacaccgg catttcgtcc aaggccatgg gcatcatgaa ctcgtttgtg aacgacattt
     7381 tcgagcgcat cgcaggtgag gcttcccgcc tggcgcatta caacaagcgc tcgaccatca
     7441 cctccaggga gatccagacg gccgtgcgcc tgctgctgcc tggggagttg gccaagcacg
     7501 ccgtgtccga gggtactaag gccatcacca agtacaccag cgctaaggat ccaccggtcg
     7561 ccaccatggc ctcctccgag gacgtcatca aggagttcat gcgcttcaag gtgcgcatgg
     7621 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     7681 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     7741 acatcctgtc ccctcagttc cagtacggct ccaaggccta cgtgaagcac cccgccgaca
     7801 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     7861 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     7921 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     7981 agaccatggg ctgggaggcc tccaccgagc ggatgtaccc cgaggacggc gccctgaagg
     8041 gcgagatcaa gatgaggctg aagctgaagg acggcggcca ctacgacgcc gaggtcaaga
     8101 ccacctacat ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc gacatcaagc
     8161 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgcgccgagg
     8221 gccgccactc caccggcgcc taactcgaga ctagtcgacg tgaggatcct ctagagtcgc
     8281 ggccgcttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     8341 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
     8401 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     8461 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     8521 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     8581 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     8641 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     8701 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     8761 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     8821 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     8881 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     8941 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     9001 tcaccatggt ggcgaccggt ggatccccca gctttttgca aaagcctagg cctccaaaaa
     9061 agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa
     9121 t
//
