LOCUS       sequence-489568-        8081 bp    DNA     circular SYN 16-OCT-2025
DEFINITION  retinoic acid response element (RARe)-driven fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Tierney MT, Polak L, Yang Y, Abdusselamoglu MD, Baek I, Stewart KS, 
            Fuchs E
  TITLE     Vitamin A resolves lineage plasticity to orchestrate stem cell 
            lineage choices.
  JOURNAL   Science. 2024 Mar 8;383(6687):eadi7342. doi: 
            10.1126/science.adi7342. Epub 2024 Mar 8.
  PUBMED    38452090
REFERENCE   2  (bases 1 to 8081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-03-8"; volume: "383(6687):eadi7342. doi"; pages: " 
            10.1126/science.adi7342. Epub 2024 Mar 8"
COMMENT     Sequence Label: RARe-RFP-PGK-H2BGFP
FEATURES             Location/Qualifiers
     source          1..8081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     448..467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     701..718
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    835..856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(871..876,877..894,895..901)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    909..925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     914..936
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     933..949
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     933..949
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     968..988
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        970..988
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1016..1242
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1243..1423
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1470..1595
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2088..2321
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2506..2550
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2799..2995
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2846..2981
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3009..3686
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             3009..3686
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(3139..3157)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3407..3426
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3601..3624
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    3733..3850
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3899..4409
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4328..4346
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4421..4798
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      4811..4820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4817..4819,4820..4822,4823..5536)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4817..4819,4820..4822,4823..5533)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4862..4883)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5123..5142)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5470..5491
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5615..5848
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5920..6041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5957..5976)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6011..6030
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6081..6216
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6236..6255)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6237..6255)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6265..6282)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6265..6281)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6274..6296)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6423..6878
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6510..6529)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6720..6741
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6904..7008
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7009..7077,7078..7869)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7227..7246)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8040..8081,1..547)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      721 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      781 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      841 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      901 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      961 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     1021 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     1081 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     1141 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     1201 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     1261 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     1321 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     1381 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     1441 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     1501 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     1561 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     1621 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     1681 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     1741 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1801 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1861 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1921 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1981 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     2041 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     2101 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     2161 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     2221 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     2281 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     2341 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     2401 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     2461 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     2521 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     2581 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     2641 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     2701 cacctcccaa ccccgagggg actcgacagt tgggtcattt gaaggttagc agcccgggta
     2761 gggttcaccg aaagttcact cgctcgagat ctgcgatctg catctcaatt agtcagcaac
     2821 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     2881 tccgccccat cgctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     2941 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     3001 tgctagccat ggcctcctcc gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca
     3061 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     3121 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     3181 gggacatcct gtcccctcag ttccagtacg gctccaaggc ctacgtgaag caccccgccg
     3241 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     3301 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     3361 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     3421 agaagaccat gggctgggag gcctccaccg agcggatgta ccccgaggac ggcgccctga
     3481 agggcgagat caagatgagg ctgaagctga aggacggcgg ccactacgac gccgaggtca
     3541 agaccaccta catggccaag aagcccgtgc agctgcccgg cgcctacaag accgacatca
     3601 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgcgccg
     3661 agggccgcca ctccaccggc gcctaagaat gcaattctcg acctcgagac aaatggcagt
     3721 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     3781 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     3841 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc ggctcgaggg
     3901 ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg
     3961 ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg
     4021 ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg
     4081 ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg
     4141 aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc
     4201 gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg
     4261 gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc
     4321 ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg
     4381 ttgaccgaat caccgacctc tctccccagg gggatctacc atgccagagc cagcgaagtc
     4441 tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg
     4501 caagaagcgc aagcgcagcc gcaaggagag ctattccatc tatgtgtaca aggttctgaa
     4561 gcaggtccac cctgacaccg gcatttcgtc caaggccatg ggcatcatga actcgtttgt
     4621 gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg
     4681 ctcgaccatc acctccaggg agatccagac ggccgtgcgc ctgctgctgc ctggggagtt
     4741 ggccaagcac gccgtgtccg agggtactaa ggccatcacc aagtacacca gcgctaagga
     4801 tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     4861 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     4921 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     4981 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     5041 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     5101 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     5161 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     5221 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     5281 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     5341 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     5401 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     5461 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5521 cgagctgtac aagtaaatgc atcggtacct ttaagaccaa tgacttacaa ggcagctgta
     5581 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5641 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     5701 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5761 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5821 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     5881 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     5941 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6001 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     6061 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6121 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6181 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     6241 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     6301 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     6361 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     6421 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6481 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6541 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6601 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     6661 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6721 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     6781 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     6841 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     6901 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6961 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7021 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7081 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     7141 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     7201 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     7261 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7321 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7381 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7441 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7501 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     7561 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7621 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7681 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7741 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7801 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7861 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7921 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7981 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     8041 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa c
//
