LOCUS       sequence-489631-       11019 bp    DNA     circular SYN 17-OCT-2025
DEFINITION  Encodes human VAV1 and N-terminal EBFP2 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Qi Y, Yu CH
  TITLE     PI(3,4,5)P3-mediated Cdc42 activation regulates macrophage podosome 
            assembly.
  JOURNAL   Cell Mol Life Sci. 2025 Mar 24;82(1):127. doi: 
            10.1007/s00018-025-05664-2.
  PUBMED    40126693
REFERENCE   2  (bases 1 to 11019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci."; date: "2025-03-24"; volume: "82(1):127. doi"; pages: " 
            10.1007/s00018-025-05664-2"
COMMENT     Sequence Label: pLenti-EBFP-VAV1-W495L
FEATURES             Location/Qualifiers
     source          1..11019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     491..510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     744..761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    878..899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(914..919,920..937,938..944)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    952..968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     957..979
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     976..992
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     976..992
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1011..1031
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1013..1031
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1059..1285
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1286..1466
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1513..1638
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2131..2364
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2549..2593
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2860..2976
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2988..3008)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2993..3322
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3173..3308
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3332..3931
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3332..3351)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3828..3848
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        4007..4310
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4311..4514
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4465..4485
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4511..4535
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4607..4616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4613..4615,4616..4618,4619..5329)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(4658..4679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    7917..8505
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7970..7990)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8388..8399)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8596..8829
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8901..9022
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8938..8957)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8992..9011
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9062..9197
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9217..9236)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9218..9236)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9246..9263)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9246..9262)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9255..9277)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9404..9859
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9491..9510)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9701..9722
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9885..9989
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9990..10058,10059..10850)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10208..10227)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
       61 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      781 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      841 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      901 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      961 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     1021 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     1081 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     1141 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     1201 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     1261 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     1321 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     1381 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     1441 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     1501 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     1561 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     1621 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     1681 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     1741 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     1801 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     1861 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     1921 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     1981 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     2041 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     2101 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     2161 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     2221 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     2281 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     2341 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     2401 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     2461 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     2521 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     2581 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     2641 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     2701 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     2761 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     2821 cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat
     2881 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2941 agaattacaa aaacaaatta caaaattcaa aattttatcg accctgtgga atgtgtgtca
     3001 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3061 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3121 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     3181 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3241 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     3301 tggaggccta ggcttttgca aaaagcttac catgaccgag tacaagccca cggtgcgcct
     3361 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     3421 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     3481 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     3541 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     3601 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     3661 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     3721 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     3781 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     3841 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     3901 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     3961 cgaaaggagc gcacgacccc atgcatcgat aagcttggga gttccgcgtt acataactta
     4021 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     4081 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     4141 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     4201 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     4261 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     4321 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     4381 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     4441 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     4501 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     4561 ttgacctcca tagaagacac cgactctaga gctagcgcta ccggtcgcca ccatggtgag
     4621 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     4681 aaacggccac aagttcagcg tgaggggcga gggcgagggc gatgccacca acggcaagct
     4741 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     4801 caccctgagc cacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     4861 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     4921 cgacggcacc tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     4981 catcgagctg aagggcgtcg acttcaagga ggacggcaac atcctggggc acaagctgga
     5041 gtacaacttc aacagccaca acatctatat catggccgtc aagcagaaga acggcatcaa
     5101 ggtgaacttc aagatccgcc acaacgtgga ggacggcagc gtgcagctcg ccgaccacta
     5161 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacagcc actacctgag
     5221 cacccagtcc gtgctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     5281 gttccgcacc gccgccggga tcactctcgg catggacgag ctgtacaaga tggagctgtg
     5341 gcgccaatgc acccactggc tcatccagtg ccgggtgctg ccgcccagcc accgcgtgac
     5401 ctgggatggg gctcaggtgt gtgaactggc ccaggccctc cgggatggtg tccttctgtg
     5461 tcagctgctt aacaacctgc taccccatgc catcaacctg cgtgaggtca acctgcgccc
     5521 ccagatgtcc cagttcctgt gccttaagaa cattagaacc ttcctgtcca cctgctgtga
     5581 gaagttcggc ctcaagcgga gcgagctctt cgaagccttt gacctcttcg atgtgcagga
     5641 ttttggcaag gtcatctaca ccctgtctgc tctgtcctgg accccgatcg cccagaacag
     5701 ggggatcatg cccttcccca ccgaggagga gagtgtaggt gatgaagaca tctacagtgg
     5761 cctgtccgac cagatcgacg acacggtgga ggaggatgag gacctgtatg actgcgtgga
     5821 gaatgaggag gcggaaggcg acgagatcta tgaggacctc atgcgctcgg agcccgtgtc
     5881 catgccgccc aagatgacag agtatgacaa gcgctgctgc tgcctgcggg agatccagca
     5941 gacggaggag aagtacactg acacgctggg ctccatccag cagcatttct tgaagcccct
     6001 gcaacggttc ctgaaacctc aagacattga gatcatcttt atcaacattg aggacctgct
     6061 tcgtgttcat actcacttcc taaaggagat gaaggaagcc ctgggcaccc ctggcgcagc
     6121 caatctctac caggtcttca tcaaatacaa ggagaggttc ctcgtctatg gccgctactg
     6181 cagccaggtg gagtcagcca gcaaacacct ggaccgtgtg gccgcagccc gggaggacgt
     6241 gcagatgaag ctggaggaat gttctcagag agccaacaac gggaggttca ccctgcggga
     6301 cctgctgatg gtgcctatgc agcgagttct caaatatcac ctccttctcc aggagctggt
     6361 gaaacacacg caggaggcga tggagaagga gaacctgcgg ctggccctgg atgccatgag
     6421 ggacctggct cagtgcgtga acgaggtcaa gcgagacaac gagacactgc gacagatcac
     6481 caatttccag ctgtccattg agaacctgga ccagtctctg gctcactatg gccggcccaa
     6541 gatcgacggg gaactcaaga tcacctcggt ggaacggcgc tccaagatgg acaggtatgc
     6601 cttcctgctc gacaaagctc tactcatctg taagcgcagg ggagactcct atgacctcaa
     6661 ggactttgta aacctgcaca gcttccaggt tcgggatgac tcttcaggag accgagacaa
     6721 caagaagtgg agccacatgt tcctcctgat cgaggaccaa ggtgcccagg gctatgagct
     6781 gttcttcaag acaagagaat tgaagaagaa gctgatggag cagtttgaga tggccatctc
     6841 caacatctat ccggagaatg ccaccgccaa cgggcatgac ttccagatgt tctcctttga
     6901 ggagaccaca tcctgcaagg cctgtcagat gctgcttaga ggtaccttct atcagggcta
     6961 ccgctgccat cggtgccggg catctgcaca caaggagtgt ctggggaggg tccctccatg
     7021 tggccgacat gggcaagatt tcccaggaac tatgaagaag gacaaactac atcgcagggc
     7081 tcaggacaaa aagaggaatg agctgggtct gcccaagatg gaggtgtttc aggaatacta
     7141 cgggcttcct ccaccccctg gagccattgg accctttcta cggctcaacc ctggagacat
     7201 tgtggagctc acgaaggctg aggctgaaca gaactggtgg gagggcagaa atacatctac
     7261 taatgaaatt ggctggtttc cttgtaacag ggtgaagccc tatgtccatg gccctcctca
     7321 ggacctgtct gttcatctct ggtacgcagg ccccatggag cgggcagggg cagagagcat
     7381 cctggccaac cgctcggacg ggactttctt ggtgcggcag agggtgaagg atgcagcaga
     7441 atttgccatc agcattaaat ataacgtcga ggtcaagcac attaaaatca tgacagcaga
     7501 aggactgtac cggatcacag agaaaaaggc tttccggggg cttacggagc tggtggagtt
     7561 ttaccagcag aactctctaa aggattgctt caagtctctg gacaccacct tgcagttccc
     7621 cttcaaggag cctgaaaaga gaaccatcag caggccagca gtgggaagca caaagtattt
     7681 tggcacagcc aaagcccgct atgacttctg cgcccgagac cgatcagagc tgtcgctcaa
     7741 ggagggtgac atcatcaaga tccttaacaa gaagggacag caaggctggt ggcgagggga
     7801 gatctatggc cgggttggct ggttccctgc caactacgtg gaggaagatt attctgaata
     7861 ctgctccgga ctcagatctc gagctcaagc ttcctcgagc accagaattc gtcgacaatc
     7921 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     7981 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     8041 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     8101 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     8161 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     8221 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     8281 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     8341 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     8401 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     8461 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattcga
     8521 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8581 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     8641 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8701 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8761 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8821 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     8881 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     8941 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9001 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     9061 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9121 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9181 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     9241 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     9301 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     9361 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     9421 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     9481 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9541 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     9601 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     9661 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     9721 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9781 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     9841 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     9901 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     9961 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
    10021 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
    10081 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
    10141 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
    10201 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    10261 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    10321 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    10381 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    10441 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    10501 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    10561 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    10621 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    10681 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    10741 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    10801 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    10861 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    10921 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    10981 actgagcgtc agaccccgta gaaaagatca aaggatctt
//
