LOCUS       sequence-489673-       11076 bp    DNA     circular SYN 17-OCT-2025
DEFINITION  Cre-mediated Cyp26b1 overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11076)
  AUTHORS   Tierney MT, Polak L, Yang Y, Abdusselamoglu MD, Baek I, Stewart KS, 
            Fuchs E
  TITLE     Vitamin A resolves lineage plasticity to orchestrate stem cell 
            lineage choices.
  JOURNAL   Science. 2024 Mar 8;383(6687):eadi7342. doi: 
            10.1126/science.adi7342. Epub 2024 Mar 8.
  PUBMED    38452090
REFERENCE   2  (bases 1 to 11076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-03-8"; volume: "383(6687):eadi7342. doi"; pages: " 
            10.1126/science.adi7342. Epub 2024 Mar 8"
COMMENT     Sequence Label: pCAG-LSL-Cyp26b1-PGK-H2BRFP
FEATURES             Location/Qualifiers
     source          1..11076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    103..224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             296..529
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(649..1326)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(649..1326)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(711..734)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(909..928)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1178..1196
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      1323..1332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1345..1722)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        complement(1734..2244)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1797..1815)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(2265..2294)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(3887..3920)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3949..4083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4250..4283)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(4312..4330)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          4343..5359
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(4397..4419)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     enhancer        5701..6080
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(6100..6116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6124..6140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6148..6154,6155..6172,6173..6178))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6193..6214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(6589..6633)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6818..7051
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7544..7669
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7716..7896
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7897..8123)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(8151..8171)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8151..8169)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8190..8206)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8214..8230
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8238..8244,8245..8262,8263..8268))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8283..8304
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8421..8438)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8592..9180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8672..8691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9351..10142,10143..10211))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9974..9993
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10212..10316)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10342..10797)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10479..10500)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10691..10710
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10924..10946
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10939..10955
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10965..10983
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      join(11004..11076,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
       61 gatgggcgga gttaggggcg ggatagctag agccagacat gataagatac attgatgagt
      121 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      181 ctattgcttt atttgtaacc attataagct gcaataaaca agttcctctc actctctgat
      241 attcatttct ttgcaagtta taaatactga ataataagat gacatgaact actactgcta
      301 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
      361 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
      421 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
      481 caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt
      541 tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct taaaggtacc
      601 gatgcatcct agggactcta gaggatcctc acgtcgacta gtctcgagtt aggcgccggt
      661 ggagtggcgg ccctcggcgc gctcgtactg ttccacgatg gtgtagtcct cgttgtggga
      721 ggtgatgtcc agcttgatgt cggtcttgta ggcgccgggc agctgcacgg gcttcttggc
      781 catgtaggtg gtcttgacct cggcgtcgta gtggccgccg tccttcagct tcagcctcat
      841 cttgatctcg cccttcaggg cgccgtcctc ggggtacatc cgctcggtgg aggcctccca
      901 gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttggtgc cgcgcagctt
      961 caccttgtag atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc
     1021 gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttcaa
     1081 gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg gagccgtact ggaactgagg
     1141 ggacaggatg tcccaggcga agggcagggg gccgcccttg gtcaccttca gcttggcggt
     1201 ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt
     1261 cacggagccc tccatgcgca ccttgaagcg catgaactcc ttgatgacgt cctcggagga
     1321 ggccatggtg gcgaccggtg gatccttagc gctggtgtac ttggtgatgg ccttagtacc
     1381 ctcggacacg gcgtgcttgg ccaactcccc aggcagcagc aggcgcacgg ccgtctggat
     1441 ctccctggag gtgatggtcg agcgcttgtt gtaatgcgcc aggcgggaag cctcacctgc
     1501 gatgcgctcg aaaatgtcgt tcacaaacga gttcatgatg cccatggcct tggacgaaat
     1561 gccggtgtca gggtggacct gcttcagaac cttgtacaca tagatggaat agctctcctt
     1621 gcggctgcgc ttgcgcttct tgccgccttt cttctgcgcc ttagtcaccg ccttcttgga
     1681 gccctttttc ggggcgggag cagacttcgc tggctctggc atggtagatc cccctgggga
     1741 gagaggtcgg tgattcggtc aacgagggag ccgactgccg acgtgcgctc cggaggcttg
     1801 cagaatgcgg aacaccgcgc gggcaggaac agggcccaca ctaccgcccc acaccccgcc
     1861 tcccgcaccg ccccttcccg gccgctgctc tcggcgcgcc ctgctgagca gccgctattg
     1921 gccacagccc atcgcggtcg gcgcgctgcc attgctccct ggcgctgtcc gtctgcgagg
     1981 gtactagtga gacgtgcggc ttccgtttgt cacgtccggc acgccgcgaa ccgcaaggaa
     2041 ccttcccgac ttaggggcgg agcaggaagc gtcgccgggg ggcccacaag ggtagcggcg
     2101 aagatccggg tgacgctgcg aacggacgtg aagaatgtgc gagacccagg gtcggcgccg
     2161 ctgcgtttcc cggaaccacg cccagagcag ccgcgtccct gcgcaaaccc agggctgcct
     2221 tggaaaaggc gcaaccccaa ccccctcgag ccatcatttg ctgccagatc ctcttctgag
     2281 atgagtttct gctcgagcac cgtagcactc aacatggcct ctgtctccgg cagaatctca
     2341 ttctggttgg agtccagacc aaagaacttg acactgaggc catccacggg gtgcaagacg
     2401 gggaccagag tgatgcgagg gaaggtccgg gtggccagct cgaagcggct agtgctggcc
     2461 agctccactg ccagcacctt caggaacagc ttggccaagt gcttgcccag gcaggtccgc
     2521 acgccaccgc caaacgggag gtaatggaag cggccatcct tatcctcact gcgtgcctga
     2581 ctgaagcggt ccgggtcaaa cacattcacg tccttgaaca cgggcgctgt gtcgtgagtg
     2641 tctcggatgc tatacatgac actccagccc ttggggatct ggaaaccatc cagttcgaag
     2701 gtctgcagca cagtgcggta gccgcctgag acgggcgtga agagtctcat gacctccttg
     2761 atgacacagt ccaggtagcg caggctgctg agcgtgtcca ggcgcagggt accctcacag
     2821 gggcagccac cgccatgcag caggccctgg gcccgcagtt cctccctcag cttctccagc
     2881 acagcagggt gttttagcag ttgcatgatc aaggatgtgc ttgcactggc cgtcgtggcg
     2941 taggctgcga agattaactc cagggttcca tccttcagct cctgcatggt catctccttg
     3001 ccatgttcct tgctgctctc aatgagaatg tccagggcgt ccgagtagtc tttgccctgg
     3061 gtacactgca gcttctcacg gatagccttc tctaggccct tctgaaggat ctgccgagct
     3121 tggatgcccc tccggtagcc actgaagggc aggtccactg gcagagagaa gacattctcc
     3181 acaaactgct ggtatacctc aaagaggtgg cccaggtcct cctcagggat gctgaagcct
     3241 agcagcacac gcacggccat tcggaaggta agtcgctggg cctcctgata tacattgatg
     3301 gcctcaggct ggctgctcca ggctcgaagt gtatcctgga tcaccagttg gatcttgggc
     3361 aggtagctct caagtgcctc atggctgaag atcttggaga agaccttgcg cttgttgcgg
     3421 tggatgtcgc caatggaatt ggccaccgtg ttgggaccca gcagtacgcg tgcgctccgc
     3481 ggccactcgg tgctcactag ctggtgttcg cccagtagga tcttgcgcac attctccgca
     3541 ccggtcacac gaatcagcgg ccgccccagt aagtgtgtct tgaaaacgtt gccatacttc
     3601 tcccggcgcg acgactggaa gccggaaccc tgtagcaacc agtgaccagt ctctccgatg
     3661 agcgggaatc ccatggagcc cttggggatg ggcagcttgc agctcttgtc gcgggtggca
     3721 gcccagcgca gctgccacag ctgctgcgac accgccagca gcagcgtcac ggacaccagg
     3781 cacgcggcga gggtggccag cgccgacacc aactccaagc cttcaaacag catggcgatc
     3841 ggccggccga attcgatcta gcttgggctg caggtcgagg gacctaataa cttcgtatag
     3901 catacattat acgaagttat attaagggtt ccggatcagc ttgatgggga tccagacatg
     3961 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     4021 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     4081 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     4141 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagagtcg
     4201 cagatcctct agagtcgcag atctgcaagc tttcatttat tcatcgcgaa taacttcgta
     4261 tagcatacat tatacgaagt tatatttaaa tgattggtgg cgggtcgacc ccctatagtg
     4321 agtcgtatta ttaattaagg atctgtagga aaaagaagaa ggcatgaaca tggttagcag
     4381 aggctctaga gccgccggtc acacgccaga agccgaaccc cgccctgccc cgtccccccc
     4441 gaaggcagcc gtccccccgc ggacagcccc gaggctggag agggagaagg ggacggcggc
     4501 gcggcgacgc acgaaggccc tccccgccca tttccttcct gccggcgccg caccgcttcg
     4561 ccccgcgccc gctagagggg gtgcggcggc gcctcccaga tttcggctcc gcacagattt
     4621 gggacaaagg aagtccctgc gccctctcgc acgattacca taaaaggcaa tggctgcggc
     4681 tcgccgcgcc tcgacagccg ccggcgctcc gggggccgcc gcgcccctcc cccgagccct
     4741 ccccggcccg aggcggcccc gccccgcccg gcacccccac ctgccgccac cccccgcccg
     4801 gcacggcgag ccccgcgcca cgccccgtac ggagccccgc acccgaagcc gggccgtgct
     4861 cagcaactcg gggagggggg tgcagggggg gttgcagccc gaccgacgcg cccacacccc
     4921 ctgctcaccc ccccacgcac acaccccgca cgcagccttt gttcccctcg cagccccccc
     4981 cgcaccgcgg ggcaccgccc ccggccgcgc tcccctcgcg cacactgcgg agcgcacaaa
     5041 gccccgcgcc gcgcccgcag cgctcacagc cgccgggcag cgcggagccg cacgcggcgc
     5101 tccccacgca cacacacacg cacgcacccc ccgagccgct ccccccgcac aaagggccct
     5161 cccggagccc ctcaaggctt tcacgcagcc acagaaaaga aacaagccgt cattaaacca
     5221 agcgctaatt acagcccgga ggagaagggc cgtcccgccc gctcacctgt gggagtaacg
     5281 cggtcagtca gagccggggc gggcggcgcg aggcggcggc ggagcggggc acggggcgaa
     5341 ggcagcgcgc agcgactccc gcccgccgcg cgcttcgctt tttatagggc cgccgccgcc
     5401 gccgcctcgc cataaaagga aactttcgga gcgcgccgct ctgattggct gccgccgcac
     5461 ctctccgcct cgcccccccc cccccccccc cccccccccc ccccccccgg cccccccccc
     5521 cccccccccc cccccccccc cccccccccc cccccccccc cccccccccg ggcccccccc
     5581 cccccccccc cccgccccca tcgctgcaca aaataattaa aaaataaata aatacaaaat
     5641 tgggggtggg gagggggggg agatggggag agtgaagcag aacgtggggc tcacctcgac
     5701 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     5761 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     5821 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     5881 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     5941 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     6001 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     6061 actagtcaat aatcaatgtc gaatccggcg cgcctcatgg tcatagctgt ttcctgtgtg
     6121 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6181 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6241 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6301 cggtttgcgt attgggcgct cttccgctcg ataccgtcga gatccgttca ctaatcgaat
     6361 ggatctgtct ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc
     6421 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
     6481 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
     6541 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
     6601 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
     6661 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
     6721 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
     6781 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
     6841 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
     6901 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
     6961 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
     7021 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
     7081 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
     7141 tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag
     7201 cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag
     7261 cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat
     7321 tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta
     7381 tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct
     7441 ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc
     7501 gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat
     7561 ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct
     7621 cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt
     7681 tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta
     7741 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
     7801 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
     7861 ctcccaggct cagatctggt ctaaccagag agacccgttt atgtatcgag ctaggcactt
     7921 aaatacaata tctctgcaat gcggcaattc agtggttcgt ccaatccatg tcagacccgt
     7981 ctgttgcctt cctaataagg cacgatcgta ccaccttact tccaccaatc ggcatgcacg
     8041 gtgctttttc tctccttgta aggcatgttg ctaactcatc gttaccatgt tgcaagacta
     8101 caagagtatt gcataagact acattaagct tgcagctcca gcttttgttc cctttagtga
     8161 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8221 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8281 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8341 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8401 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8461 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8521 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8581 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8641 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8701 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8761 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8821 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8881 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8941 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9001 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9061 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9121 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9181 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9241 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9301 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9361 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9421 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9481 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9541 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9601 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9661 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9721 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9781 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9841 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9901 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9961 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10021 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10081 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10141 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10201 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10261 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10321 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
    10381 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
    10441 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
    10501 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
    10561 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
    10621 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
    10681 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
    10741 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
    10801 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    10861 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    10921 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
    10981 agggcgaatt gggtacgtcc ctaggcctcc aaaaaagcct cctcactact tctggaatag
    11041 ctcagaggcc gaggcggcct cggcctctgc ataaat
//
