LOCUS       sequence-489674-        8473 bp    DNA     circular SYN 17-OCT-2025
DEFINITION  Control for epicenter-driven fluorescent reporters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Ge Y, Gomez NC, Adam RC, Nikolova M, Yang H, Verma A, Lu CP, Polak 
            L, Yuan S, Elemento O, Fuchs E
  TITLE     Stem Cell Lineage Infidelity Drives Wound Repair and Cancer.
  JOURNAL   Cell. 2017 May 4;169(4):636-650.e14. doi: 
            10.1016/j.cell.2017.03.042. Epub 2017 Apr 20.
  PUBMED    28434617
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.03.042"; journalName: "Cell"; date: "2017-05-4-
            4"; volume: "169"; issue: "4"; pages: "636-650.e14"
COMMENT     Sequence Label: Empty(Ctrl)-eGFP-PGK-H2BRFP
FEATURES             Location/Qualifiers
     source          1..8473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    103..224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(114..133)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     168..187
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             296..529
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        596..791
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      642..777
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      813..822
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(819..821,822..824,825..1538)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(819..821,822..824,825..1535)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(864..885)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1125..1144)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1472..1493
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(1583..2260)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(1583..2260)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(1645..1668)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1843..1862)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2112..2130
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2257..2266
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2279..2656)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        complement(2668..3178)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2731..2749)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    3227..3344
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(3440..3688)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(3498..3517)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(3668..3688)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(3986..4030)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4215..4448
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4941..5066
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5113..5293
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5294..5520)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(5548..5568)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5548..5566)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5587..5603)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5587..5603)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5600..5622)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5611..5627
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5635..5641,5642..5659,5660..5665))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5680..5701
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5818..5835)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5989..6577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6069..6088)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6748..7539,7540..7608))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7371..7390
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7609..7713)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7739..8194)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7876..7897)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8088..8107
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8321..8343
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8335..8352
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8336..8352
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8362..8381
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8362..8380
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      join(8401..8473,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
       61 gatgggcgga gttaggggcg ggatagctag agccagacat gataagatac attgatgagt
      121 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      181 ctattgcttt atttgtaacc attataagct gcaataaaca agttcctctc actctctgat
      241 attcatttct ttgcaagtta taaatactga ataataagat gacatgaact actactgcta
      301 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
      361 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
      421 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
      481 caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt
      541 tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct taaaggcatc
      601 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
      661 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
      721 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
      781 gcttttgcaa aaagctgggg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc
      841 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      901 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      961 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     1021 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1081 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1141 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1201 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     1261 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     1321 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1381 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     1441 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     1501 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagaggat
     1561 cctcacgtcg actagtctcg agttaggcgc cggtggagtg gcggccctcg gcgcgctcgt
     1621 actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccagcttg atgtcggtct
     1681 tgtaggcgcc gggcagctgc acgggcttct tggccatgta ggtggtcttg acctcggcgt
     1741 cgtagtggcc gccgtccttc agcttcagcc tcatcttgat ctcgcccttc agggcgccgt
     1801 cctcggggta catccgctcg gtggaggcct cccagcccat ggtcttcttc tgcattacgg
     1861 ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct
     1921 gcagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct
     1981 cccacttgaa gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct
     2041 tcacgtaggc cttggagccg tactggaact gaggggacag gatgtcccag gcgaagggca
     2101 gggggccgcc cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct
     2161 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg cgcaccttga
     2221 agcgcatgaa ctccttgatg acgtcctcgg aggaggccat ggtggcgacc ggtggatcct
     2281 tagcgctggt gtacttggtg atggccttag taccctcgga cacggcgtgc ttggccaact
     2341 ccccaggcag cagcaggcgc acggccgtct ggatctccct ggaggtgatg gtcgagcgct
     2401 tgttgtaatg cgccaggcgg gaagcctcac ctgcgatgcg ctcgaaaatg tcgttcacaa
     2461 acgagttcat gatgcccatg gccttggacg aaatgccggt gtcagggtgg acctgcttca
     2521 gaaccttgta cacatagatg gaatagctct ccttgcggct gcgcttgcgc ttcttgccgc
     2581 ctttcttctg cgccttagtc accgccttct tggagccctt tttcggggcg ggagcagact
     2641 tcgctggctc tggcatggta gatccccctg gggagagagg tcggtgattc ggtcaacgag
     2701 ggagccgact gccgacgtgc gctccggagg cttgcagaat gcggaacacc gcgcgggcag
     2761 gaacagggcc cacactaccg ccccacaccc cgcctcccgc accgcccctt cccggccgct
     2821 gctctcggcg cgccctgctg agcagccgct attggccaca gcccatcgcg gtcggcgcgc
     2881 tgccattgct ccctggcgct gtccgtctgc gagggtacta gtgagacgtg cggcttccgt
     2941 ttgtcacgtc cggcacgccg cgaaccgcaa ggaaccttcc cgacttaggg gcggagcagg
     3001 aagcgtcgcc ggggggccca caagggtagc ggcgaagatc cgggtgacgc tgcgaacgga
     3061 cgtgaagaat gtgcgagacc cagggtcggc gccgctgcgt ttcccggaac cacgcccaga
     3121 gcagccgcgt ccctgcgcaa acccagggct gccttggaaa aggcgcaacc ccaaccccct
     3181 cgagccgcgg ccaaagtgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt
     3241 ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc
     3301 tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg
     3361 ccatttgtct cgaggtcgag aattaaaaac aacaagatga agagcaccaa ctcgagttgg
     3421 tgctcttcat cttgttgccg gtgtttcgtc ctttccacaa gatatataaa gccaagaaat
     3481 cgaaatactt tcaagttacg gtaagcatat gatagtccat tttaaaacat aattttaaaa
     3541 ctgcaaacta cccaagaaat tattactttc tacgtcacgt attttgtact aatatctttg
     3601 tgtttacagt caaattaatt ccaattatct ctctaacagc cttgtatcgt atatgcaaat
     3661 atgaaggaat catgggaaat aggccctcgg tgaagggggc ggccgctcga ggctagtctc
     3721 gtgatcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc
     3781 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
     3841 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
     3901 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
     3961 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt
     4021 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt
     4081 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag
     4141 atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc
     4201 aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg
     4261 cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag
     4321 ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg
     4381 tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca
     4441 aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg
     4501 gtgctactcc taatggttca atttttacta ctttatattt atataattca cttctccaat
     4561 tgtccctcat atctcctcct ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc
     4621 ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct
     4681 atcctttgat gcacacaata gagggttgct actgtattat ataatgatct aagttcttct
     4741 gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt
     4801 tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt
     4861 tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta
     4921 attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta
     4981 gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca
     5041 gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg
     5101 ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
     5161 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
     5221 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
     5281 accagagaga cccgtttatg tatcgagcta ggcacttaaa tacaatatct ctgcaatgcg
     5341 gcaattcagt ggttcgtcca atccatgtca gacccgtctg ttgccttcct aataaggcac
     5401 gatcgtacca ccttacttcc accaatcggc atgcacggtg ctttttctct ccttgtaagg
     5461 catgttgcta actcatcgtt accatgttgc aagactacaa gagtattgca taagactaca
     5521 ttaagcttgc agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     5581 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     5641 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     5701 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     5761 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     5821 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5881 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5941 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6001 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6061 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6121 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6181 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6241 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6301 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6361 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6421 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6481 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6541 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6601 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6661 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6721 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6781 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6841 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6901 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6961 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7021 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7081 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7141 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7201 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7261 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7321 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7381 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7441 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7501 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7561 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7621 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7681 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
     7741 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     7801 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     7861 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     7921 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     7981 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     8041 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     8101 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     8161 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
     8221 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     8281 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     8341 acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg tacgtcccta
     8401 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
     8461 cctctgcata aat
//
