LOCUS       sequence-489750-       11039 bp    DNA     circular SYN 07-NOV-2025
DEFINITION  PHF19 donor plasmid with puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11039)
  AUTHORS   Much C, Rajkumar SM, Chen L, Cohen JM, Gade AR, Pitt GS, Long Y
  TITLE     Distinct specificity and functions of PRC2 subcomplexes in human 
            stem cells and cardiac differentiation.
  JOURNAL   Mol Cell. 2025 Aug 7:S1097-2765(25)00615-X. doi: 
            10.1016/j.molcel.2025.07.014.
  PUBMED    40803325
REFERENCE   2  (bases 1 to 11039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Aug 7:S1097-2765(25)00615-X. doi: 
            10.1016/j.molcel.2025.07.014."
COMMENT     Sequence Label: pUC19_PHF19_HTC3xF_Puro_donor
FEATURES             Location/Qualifiers
     source          1..11039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2734..2754
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             2767..3654
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             3655..3720
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    6030..6151
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6152..6273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6274..6395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6398..6509
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6612..6645
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6646..7156
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7075..7093
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7166..7765
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7166..7185)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7662..7682
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7801..8025
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    8026..8059
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(8818..8834)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8818..8834)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8831..8853)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8842..8858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8866..8872,8873..8890,8891..8896))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8911..8932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9049..9066)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9220..9808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9300..9319)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9979..10770,10771..10839))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10602..10621
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10840..10944)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11012..11030
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctctaa ttgagaacag
      421 aaggggggat tacaaaagac agatgaccca gtctacacta gctacaagcc acccagtttt
      481 cctttctagg tgaattttct ctggacagat ctcaggttag ggatggttgc aggggttggg
      541 ggtaaaaaca aaacccaact gcttgcctgg agaagatcct ccatccagag cacatggact
      601 gtgtttgctt aaggaataaa acctcagcct cctgagacag ggagtggttc aggcccgctg
      661 ggtaggggac tttgttctgt gtgggttgag agatccattc tctttaaaag gtagtgtggt
      721 atgggccatg gctctgcctt tggcccatca gcctcttctc cttgccacac ctgtttccct
      781 atctgtaaga tgtgaataat aataatgtct ccctcatagg atggtcacga ggaatcagtg
      841 agataataac caagtctggc ttatagcaca gggtacagaa atggtggttt ccttccttcc
      901 cctgatgggc tttctgaagc agggaagcaa gaagcaaaaa ccctgttctt tctctcccag
      961 tgtcagggga agctgatgga gaatcgagct ctggatccag ggactcggga ctcctatggt
     1021 gccaccagcc acctccccaa caagggggcc ctggcgaagg tcaagaacaa cttcaaagac
     1081 ttgatgtcca aactgacgga gggccagtat gtgctgtgcc ggtggacaga tggcctgtac
     1141 tacctcggga agatcaagag ggtcagcagc tctaagcaaa gctgcctcgt gactttcgaa
     1201 gataattcca aatactgggt cctatggaag gacatacagc atgccggtgt tccaggagag
     1261 gagcccaagt gcaacatctg cctagggaag acatcagggc cgctgaatga gatcctcatc
     1321 tgcgggaagt gtggcctggg ttaccaccag cagtgccaca tccccatagc gggcagtgct
     1381 gaccagcccc tgctcacacc ttggttctgc cgacgctgca tcttcgcact ggctgtgcgg
     1441 aaaggcggcg cgctgaagaa gggcgccatc gccaggacgc tgcaggccgt gaagatggtg
     1501 ctgtcctacc agcccgagga gctcgagtgg gactcgcccc atcgcaccaa ccagcagcaa
     1561 tgctactgct actgcggcgg gcccggagaa tggtacctgc ggatgctgca atgttaccgg
     1621 tgcaggcagt ggttccacga ggcctgcacc cagtgcctca atgagcccat gatgtttgga
     1681 gaccggtttt acctgttctt ctgctccgtg tgtaaccagg gcccagagta catcgagagg
     1741 ctgcccctgc gatgggtgga tgtggttcac ctggccctct ataatctggg ggtacagagc
     1801 aagaagaagt actttgactt tgaggagatt ctggcctttg tcaaccacca ctgggagctc
     1861 ctgcagcttg gcaagctcac cagcacccca gtgacagatc gaggaccaca tctcctcaac
     1921 gctctgaaca gttataaaag ccggttcctc tgcggcaagg agatcaagaa gaagaagtgc
     1981 atcttccgcc tgcgcatccg cgtcccaccc aacccgccag ggaagctgct gcctgacaaa
     2041 ggactgctgc caaatgagaa cagcgcctcc tctgagctgc gtaagagagg aaagagcaag
     2101 cctggtttgt tgcctcacga attccagcag cagaaaaggc gagtttatag aagaaaaaga
     2161 tcaaagtttt tgctggaaga tgctattccc agtagtgact tcacctcagc ctggagcacc
     2221 aaccaccacc tggctagcat atttgacttc acgctggatg aaattcaaag tttaaaaagt
     2281 gccagctcag gccagacctt cttctcagat gtcgactcca ccgacgctgc cagcacctct
     2341 ggctctgcct ccaccagcct ctcctatgac tccagatgga cagtgggcag ccgaaagagg
     2401 aagctggcag ccaaggcata catgcccctg cgggcaaagc ggtgggcagc tgagctggat
     2461 ggacgctgcc cctcggacag cagtgcagag ggggcttcag tccccgagcg gccagacgaa
     2521 ggcattgaca gccacacatt tgagagcatc agtgaagatg actcatccct gtcccacctc
     2581 aagtcatcta tcaccaacta ctttggtgca gctgggcggt tggcctgtgg ggagaagtac
     2641 caggtgttgg ctcggagggt cacacctgag ggcaaggttc agtacctggt ggagtgggaa
     2701 gggaccaccc cttacaccgg tgagccaacc actgaggatc tgtactttca gagcgataac
     2761 gcgatcgcag aaatcggtac tggctttcca ttcgaccccc attatgtgga agtcctgggc
     2821 gagcgcatgc actacgtcga tgttggtccg cgcgatggca cccctgtgct gttcctgcac
     2881 ggtaacccga cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc accgacccat
     2941 cgctgcattg ctccagacct gatcggtatg ggcaaatccg acaaaccaga cctgggttat
     3001 ttcttcgacg accacgtccg cttcatggat gccttcatcg aagccctggg tctggaagag
     3061 gtcgtcctgg tcattcacga ctggggctcc gctctgggtt tccactgggc caagcgcaat
     3121 ccagagcgcg tcaaaggtat tgcatttatg gagttcatcc gccctatccc gacctgggac
     3181 gaatggccag aatttgcccg cgagaccttc caggccttcc gcaccaccga cgtcggccgc
     3241 aagctgatca tcgatcagaa cgtttttatc gagggtacgc tgccgatggg tgtcgtccgc
     3301 ccgctgactg aagtcgagat ggaccattac cgcgagccgt tcctgaatcc tgttgaccgc
     3361 gagccactgt ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc gaacatcgtc
     3421 gcgctggtcg aagaatacat ggactggctg caccagtccc ctgtcccgaa gctgctgttc
     3481 tggggcaccc caggcgttct gatcccaccg gccgaagccg ctcgcctggc caaaagcctg
     3541 cctaactgca aggctgtgga catcggcccg ggtctgaatc tgctgcaaga agacaacccg
     3601 gacctgatcg gcagcgagat cgcgcgctgg ctgtcgacgc tcgagatttc cggcgactac
     3661 aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag
     3721 tgaacgcgtc tagcccccgg gggtgccagg ggtcctgaaa accaaaggag gagcagcaga
     3781 agccataggc tccccagctt tctccaggct ggggtgggag aaggaagcag gacagagctg
     3841 caagtgcctg gcagaatgcc ctgcctgcct gcctgccagg ccaaggcctg cgtctctctg
     3901 ctgtaccagc tctgttccag ggcctcctca ggctcgttac ccctgtgcct gtgtctctac
     3961 acactccaca ccccctcaaa ctctgtttat ctgttctctg accttgtgtc ccctgcgctg
     4021 ggacccttcc tcctgaggcc caggtctttg tccccagttg tgtgccttga cctctctcgc
     4081 ccctttctgg gtgtgttcgc acatcctgtg tgtgcacagc tgtccctcca ctggatcccc
     4141 ttcacacgtg acccgtgggg cagccagtcc tcccagggac tacataacag gcacctttga
     4201 gagagcatgg gagaaggtgg ataagaggat gctgctcagt gcttttctct tccactttcc
     4261 tgccactccc cactaccctc ggagagaggt ggtgggatgg gagagagccc ctgtgaaagc
     4321 ctgtgaggat ctgaagagta aagggctggg tctgcctcag aaggcaccag caccagggcc
     4381 caggtattaa ggctgagagt gaaggctgcc aatgtcagct ttggaggtcc cagaagtctt
     4441 ctgttctctg gcctcacccc ctcagtcgcc atagagctgg gcctggcctt gctggaatgg
     4501 aggcatcctt ccaaacctgg gggacggggg tggggggtgg tagtggtggg agggaaacca
     4561 tgtcttgcta aacctgtttc tggtgcctcc catccccaga cccaccagac accacacagc
     4621 agacaataca cacccactcg cacaagcttc catccacatg tgttgtactt tcagctctag
     4681 gcatgcagac aaccccacac ggccacacca ccacatgccc aagtgtacac acacagagcc
     4741 acaccgtccc tctgggcctg ctggctcctc ccttggcttt cccttggccc acttccaggg
     4801 cccaggtgct gcaactaaat gtgaaagctc agtggccgct ccttctttca gcccatcaac
     4861 cagcattggt cccataggga agcacaggtg actcaccctc tttcatatcc cttgccctgc
     4921 cctgaaatgg acaatcactt tttgggatag gttgaaattt ttaaagagcc tgcatcattc
     4981 ggttccctca aagggaagcc cttgccagtg ggggtttgaa agagaatttt tggaaccaac
     5041 attcaaattc tgcctcatct ggagggaaac caaaattggg agggggaaga ggacccctga
     5101 tgttttgctg cttccagaga tattagaaac tgactcactt gattggaaaa tggacaaaag
     5161 tgccttgacg tggagggtgg gcaccagatg gggaccagcc ttgccaactg ctgctgtggc
     5221 ctccagcttg gctggttttg caggccgcca gcaggaaggc gaaggtggta gtacagcaag
     5281 aggcactggc ggggcagcag gcctgcagga gctgtttttc cattgctagg cctgacccct
     5341 ctctacctgt gagcgttcag ggggtccctg agatagttta gatgcccccc ccatcttaga
     5401 cctcagctcc cacagtgcct tttaaggggg acctcacctc ctgtgcacag cccacccact
     5461 ttcctctgct tccctggcac agcccaggca tagacgagct ggcgttggac ccagttcttc
     5521 ccccttttca gccccacagc tgctgccaca ggggccaact agggccaggt ggaaggggag
     5581 ctgagaagcc aacccctagc ccaggggtgc tgtgggaact gggatccaat ttgtagcttc
     5641 ctgcctggct tcagagagcc cagcaacctt ctaggcctgc tttccagact tctgagatag
     5701 cctgggatga gcaatcctgt tacagtacat ctggaccttc cctacctggg ctctggggag
     5761 gctgtgggcc tggagaggga aaaggaggga gggggtgtct gcaccacctg ggaagatagc
     5821 acaaggccta atgaggtcac cctgactccc caccccagca tttcattcat accagataat
     5881 agctgcatta ctgccaactg accttataac cctctgcacc ttcaaaaaga ttcatggttt
     5941 ttaattgctg cttttaataa catttgttaa agttataatt aatgtgtctg atttatgatt
     6001 taaaacctcc ctttgaacaa tcaggtacca acttgtttat tgcagcttat aatggttaca
     6061 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6121 gtggtttgtc caaactcatc aatgtatctt aaacttgttt attgcagctt ataatggtta
     6181 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6241 ttgtggtttg tccaaactca tcaatgtatc ttaaacttgt ttattgcagc ttataatggt
     6301 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6361 agttgtggtt tgtccaaact catcaatgta tcttagactg tgccttctag ttgccagcca
     6421 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     6481 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     6541 gggggtgggg tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgcg
     6601 gccgcgatat cataacttcg tataatgtat gctatacgaa gttatggggt tggggttgcg
     6661 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     6721 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     6781 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
     6841 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
     6901 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
     6961 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     7021 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     7081 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     7141 cgacctctct ccccagcaat tcaccatgac cgagtacaag cccacggtgc gcctcgccac
     7201 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     7261 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     7321 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     7381 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     7441 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     7501 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     7561 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     7621 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     7681 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     7741 catgacccgc aagcccggtg cctgactcga gtctagacca attggtttaa accctgcagg
     7801 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     7861 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     7921 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     7981 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggataac ttcgtataat
     8041 gtatgctata cgaagttatc ctaggtggcc cctgttctca gactcagaat ctctctgctc
     8101 atgttccagc ctggctgtca gaccaggggg cagggggatt gctggagaat tcccttcttg
     8161 tccctcatgc ccaaagctgg tggtgggagg ggccagagcc tggtcttgca ggcaccatcc
     8221 ctagggtcaa catcatccaa ctagttcttt agaaaagtgc tctgagagcc tacaaaacaa
     8281 aaaagtcagg tttgagagga tcacagagga gcatctgact tgtgcttctg tcctcaacat
     8341 tctgaccaag ttgccatcca tcggtccctg gctgtacgac tccaggtgac taggagtaca
     8401 ccctgtccag gaactgccat ccttcttccc cttctagccc tgctttctgg aacatacgga
     8461 gacaaaaatg gaggaggaag actatgttcc aggttggctt ttccccagcc ctttctcact
     8521 tgctccttcc tatctgtacc ccaggtcagc agctctaagc aaagctgcct cgtgactttc
     8581 gaagataatt ccaaatactg ggtcctatgg aaggacatac agcatggtga gttttcctaa
     8641 catttcgggt agcccccaac ctccccaggt cttctctcct ctgctgcctt catttcttcc
     8701 ttctgtcctt ccatctctta ctcctggcat ctgcccttga ccatgaaccc caagcccacc
     8761 tgtgatgatg atcaaatctg gttgtatatt gacttaggaa agcttggcgt aatcatggtc
     8821 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8881 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8941 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     9001 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     9061 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     9121 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9181 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9241 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9301 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9361 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9421 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9481 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9541 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9601 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9661 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9721 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9781 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9841 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9901 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9961 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    10021 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    10081 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    10141 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    10201 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    10261 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    10321 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10381 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10441 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10501 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10561 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10621 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10681 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10741 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10801 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10861 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10921 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    10981 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
