LOCUS       sequence-489808-        8891 bp    DNA     circular SYN 20-OCT-2025
DEFINITION  PHF1 donor plasmid with blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Much C, Rajkumar SM, Chen L, Cohen JM, Gade AR, Pitt GS, Long Y
  TITLE     Distinct specificity and functions of PRC2 subcomplexes in human 
            stem cells and cardiac differentiation.
  JOURNAL   Mol Cell. 2025 Aug 7:S1097-2765(25)00615-X. doi: 
            10.1016/j.molcel.2025.07.014.
  PUBMED    40803325
REFERENCE   2  (bases 1 to 8891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Aug 7:S1097-2765(25)00615-X. doi: 
            10.1016/j.molcel.2025.07.014."
COMMENT     Sequence Label: pUC19_PHF1_HTC3xF_BSD_donor
FEATURES             Location/Qualifiers
     source          1..8891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1447..1461
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     CDS             2719..2739
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             2752..3639
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             3640..3705
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    4062..4183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4184..4305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4306..4427
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4430..4541
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4644..4677
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4678..5188
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5107..5125
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5198..5596
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5637..5861
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    5862..5895
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6670..6686)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6670..6686)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6683..6705)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6694..6710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6718..6724,6725..6742,6743..6748))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6763..6784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6901..6918)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7072..7660)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7152..7171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7831..8622,8623..8691))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8454..8473
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8692..8796)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8864..8882
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctctgg aagctgggaa
      421 actagcaact aggtttgggg tcggggggag ggctgttagt taccgagagg agagccagtc
      481 actagagttg gaagaggact caaaaagctg gtgatgggtg cccagtggtt tcctatcaag
      541 gtgtctcagg gatttggaga tgggggcaca gatacttcat tcttgatggc aaaataggca
      601 aagaaaatgg ggaggctggc cgggcccggt ggctcacgcc tgtaatccca gcactttggg
      661 aagctgaggt gggtggatca tgaggtcagg agttcgagac cagtttggcc aacatggcca
      721 acacctcatc tctactaaaa atacaaaaat tagccaggca tggtggcatg cgcctgtaat
      781 cccacctact tgggagactg aggcaggaga attgcctgaa cccgggaggt ggaggttgca
      841 gtgagccaag attgtgccac tgcactctgg cctgggcgac agagcaaaac tccatctcag
      901 gaaaaaaaaa aaaaaagaaa aagaaaatgg ggaaggtttc tcagcattca taaccttctc
      961 ctccccattt cttttctggc taggcccccc caggatgcaa tggcgcagcc cccccggctg
     1021 agccgctctg gtgcctcctc actttgggac ccagcttctc ctgctcccac ctctggcccc
     1081 aggcctcggc tttgggaggg tcaagatgtg ctggccagat ggactgatgg gctgctatac
     1141 ttgggtacca tcaaaaaggt ggacagtgct agggaggtgt gtctggtcca gtttgaggat
     1201 gattcgcagt ttctggttct atggaaagac attagccctg ctgccctccc tggagaggaa
     1261 ctcctctgtt gtgtctgtcg ctctgagact gtggtccctg ggaaccggct ggtcagctgt
     1321 gagaagtgtc gccatgctta tcaccaggac tgccatgttc ccagggctcc agcccctgga
     1381 gagggagagg gcacatcctg ggtatgccgc cagtgtgtct ttgcgatcgc caccaagagg
     1441 ggaggtgccc tgaagaaggg cccctatgcc cgggccatgc tgggtatgaa gctttctctg
     1501 ccatatggac tgaaggggct ggactgggat gctggacatc tgagcaaccg acagcagagt
     1561 tactgttact gtggtggccc tggggagtgg aacctgaaaa tgctgcagtg ccggagctgc
     1621 ctgcagtggt tccatgaggc ctgcacccag tgtctgagca agcccctcct ctatggggac
     1681 aggttctatg aatttgaatg ctgtgtgtgt cgcgggggcc ctgagaaagt ccggagacta
     1741 cagcttcgct gggtggatgt ggcccatctt gtcctgtatc acctcagtgt ttgctgtaag
     1801 aagaaatact ttgattttga tcgtgagatc ctccccttca cttctgagaa ttgggacagt
     1861 ttgctcctgg gggagctttc agacaccccc aaaggagaac gttcttccaa gctcctctct
     1921 gctcttaaca gccacaagga ccgtttcatt tcagggagag agattaagaa gaggaaatgt
     1981 ttgtttggtc tccatgctcg gatgcctccc cctgtggagc cccctactgg agatggagca
     2041 ctcaccagct tcccttcagg gcagggccct gggggagggg tctcacgtcc cctggggaag
     2101 cgccggaggc cggagccaga gcccctgagg aggaggcaga aggggaaagt ggaggagctg
     2161 gggccaccct cagcagtgcg caatcagccc gagccccagg agcagaggga gcgggctcat
     2221 ctgcagaggg cactgcaggc ctcagtgtct ccaccatccc ccagccctaa ccagagttac
     2281 cagggcagca gcggctacaa cttccggccc acagatgccc gctgcctgcc cagcagcccc
     2341 atccggatgt ttgcttcctt ccacccttct gccagcaccg cagggacctc tggggacagt
     2401 ggacccccag acaggtcacc cctggaactt cacattggtt tccccacaga catccctaaa
     2461 agtgcccccc actcgatgac tgcctcatct tcctcagttt catccccatc cccaggtctt
     2521 cctagacgct cagcaccccc ttctcccctg tgccgtagtt tgtctcctgg gactggggga
     2581 ggagtccgag gtggggttgg ttacctgtcc cgaggggacc ctgtccgggt ccttgctcgg
     2641 agagtacggc ctgatggctc tgtgcagtac ctggttgagt ggggaggagg gggcatcttc
     2701 accggtgagc caaccactga ggatctgtac tttcagagcg ataacgcgat cgcagaaatc
     2761 ggtactggct ttccattcga cccccattat gtggaagtcc tgggcgagcg catgcactac
     2821 gtcgatgttg gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc
     2881 tcctacgtgt ggcgcaacat catcccgcat gttgcaccga cccatcgctg cattgctcca
     2941 gacctgatcg gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac
     3001 gtccgcttca tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt
     3061 cacgactggg gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa
     3121 ggtattgcat ttatggagtt catccgccct atcccgacct gggacgaatg gccagaattt
     3181 gcccgcgaga ccttccaggc cttccgcacc accgacgtcg gccgcaagct gatcatcgat
     3241 cagaacgttt ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc
     3301 gagatggacc attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc
     3361 ttcccaaacg agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa
     3421 tacatggact ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc
     3481 gttctgatcc caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct
     3541 gtggacatcg gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc
     3601 gagatcgcgc gctggctgtc gacgctcgag atttccggcg actacaaaga ccatgacggt
     3661 gattataaag atcatgacat cgattacaag gatgacgatg acaagtgaac gcgtacagcc
     3721 tgcctctgcc cagctcccca ttcacacaca ccggcacttt cataccctga cctctgacct
     3781 cacctacagc tgggatgtac ctggagagat agggggtagt tctccctact gcccaggctg
     3841 gaatccaaga gtggggagtg gggaagaggc cctcttctct accctccttc atgattcctg
     3901 acccctccca tccttcccat ttcctttgat gttattttgt tacagctttt taaatatttt
     3961 ttaaaattat ttaacccctg ggggcagaga ctgaggaggg aggatgataa gggatcccgg
     4021 actctgtatg attgaaataa agagaaataa acaaaggtac caacttgttt attgcagctt
     4081 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4141 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaacttgt ttattgcagc
     4201 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4261 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaactt gtttattgca
     4321 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4381 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttagac tgtgccttct
     4441 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     4501 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     4561 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat
     4621 agcaggcatg cggccgcgat atcataactt cgtataatgt atgctatacg aagttatggg
     4681 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     4741 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     4801 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     4861 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     4921 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
     4981 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
     5041 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
     5101 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
     5161 tgaccgaatc accgacctct ctccccagca attcaccatg gccaagcctt tgtctcaaga
     5221 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
     5281 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
     5341 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
     5401 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
     5461 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
     5521 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
     5581 tgtgtgggag ggctaagctg actcgagtct agaccaattg gtttaaaccc tgcaggctgt
     5641 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     5701 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5761 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5821 agacaatagc aggcatgctg gggatgcggt gggctctatg gataacttcg tataatgtat
     5881 gctatacgaa gttatcctag ggtaagacct tctacctctg accttcttcc tagttccctt
     5941 atctaattct ggttcccatt tcatcctgtt tgctcaccca ttccaggtgg acagtgctag
     6001 ggaggtgtgt ctggtccagt ttgaggatga ttcgcagttt ctggttctat ggaaagacat
     6061 tagccctggt aagactctag agacctgaga ttgcacatcc catggaaaac aaacctggcc
     6121 tagaggggca gaaagagact taaaggcagg ccctgtgaca ctgtgttctc tcacagctgc
     6181 cctccctgga gaggaactcc tctgttgtgt ctgtcgctct gagactgtgg tccctgggaa
     6241 ccggctggtc agctgtgaga agtgtcgcca tggtgagagg gcaggtcacc tgaatggtcc
     6301 agcttgctct tccctccagg atggtctcta tatcacctgt cctggcctta gtccccaagc
     6361 cactgctctg gccaggctgc ttagccttat cttagtcctc atccgctttc agcccaggga
     6421 gaagcagcca tggaaagggg tggtataacc tttgcaggca gaagatggtt taaatgcatc
     6481 cttttctaac catatgacct cggacaagtc ccttaacctc taggagcctc agttccctga
     6541 cttgtaaaat aaggataatg caccccctca tcaagaccat ggtcagggat taaatgagat
     6601 gggtaaagac tattcctgtc ccaataccaa gcacacacag gtatgcaata aaagcttggc
     6661 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6721 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6781 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6841 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6961 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7021 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7081 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7141 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7201 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7261 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7321 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7381 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7441 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7501 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7561 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7621 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7681 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7741 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7801 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7861 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7921 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7981 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8041 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8101 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8161 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8221 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8281 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8341 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8401 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8461 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8521 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8581 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8641 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8701 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8761 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8821 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     8881 gccctttcgt c
//
