LOCUS       sequence-489818-         650 bp    DNA     linear   UNA 20-OCT-2025
DEFINITION  In vitro transcription of wild-type FAM136A downstream of SP6 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Harhai M, Foged MM, Zarges C, Landoni JC, Chollet S, Simonelli M, 
            Recazens E, Lisci M, Laban N, Manley S, Riemer J, Lopez-Escamez JA, 
            Lysakowski A, Jourdain AA
  TITLE     An updated inventory of genes essential for oxidative 
            phosphorylation identifies a mitochondrial origin in familial 
            Meniere's disease.
  JOURNAL   Cell Rep. 2025 Jul 25;44(8):116069. doi: 
            10.1016/j.celrep.2025.116069.
  PUBMED    40714634
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2025-07-25"; volume: "44(8):116069. doi"; pages: " 
            10.1016/j.celrep.2025.116069"
COMMENT     Sequence Label: pTwist Amp High Copy FAM136A-HA WT
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..43
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     35..52
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     36..52
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        60..78
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     60..77
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             515..541
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(591..610)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(592..610)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(629..645)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(629..645)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgga
       61 tttaggtgac actatagaat acgaattcga gctcggtacc atggcagaat tgcagcagtt
      121 acgtgttcag gaggcagtcg agagcatggt gaaaagctta gagcgggaga atatacgtaa
      181 gatgcaggga ttgatgttcc gatgctctgc aagctgttgc gaagacagtc aggcctcaat
      241 gaaacaggtc catcaatgca ttgagagatg tcatgttcca ctggcccagg cccaggctct
      301 cgtcacaagt gaattagaga agttccagga taggttggcg aggtgtacta tgcactgcaa
      361 tgacaaggcc aaggacagta tcgatgctgg gagtaaggag cttcaggtga agcagcaact
      421 ggacagctgt gtgacaaagt gcgtagatga ccacatgcac ttaattccta caatgaccaa
      481 gaagatgaag gaagccttgc tgtccatcgg gaaataccca tacgatgttc cagattacgc
      541 ttaatagtga ggatcctcta gagtcgacct gcaggcatgc aagcttgtct ccctatagtg
      601 agtcgtatta gagcttggcg taatcatggt catagctgtt tcctgtgtga
//
