LOCUS       sequence-489853-        6561 bp    DNA     circular SYN 21-OCT-2025
DEFINITION  Nuclear localized tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Economo MN, Viswanathan S, Tasic B, Bas E, Winnubst J, Menon V, 
            Graybuck LT, Nguyen TN, Smith KA, Yao Z, Wang L, Gerfen CR, 
            Chandrashekar J, Zeng H, Looger LL, Svoboda K
  TITLE     Distinct descending motor cortex pathways and their roles in 
            movement.
  JOURNAL   Nature. 2018 Nov;563(7729):79-84. doi: 10.1038/s41586-018-0642-9. 
            Epub 2018 Oct 31.
  PUBMED    30382200
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 21, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0642-9"; journalName: "Nature"; date: "2018-11";
            volume: "563"; issue: "7729"; pages: "79-84"
COMMENT     Sequence Label: pAAV-CAG-H2B tdTomato
FEATURES             Location/Qualifiers
     source          1..6561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     364..386
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     448..467
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             530..907
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      920..929
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             926..2356
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    2372..2960
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2425..2445)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2843..2854)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3003..3124
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3014..3033)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3068..3087
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3286..3415
                     /label=AAV2 ITR
     rep_origin      complement(3652..4240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3732..3751)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4411..5202,5203..5271))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5034..5053
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5272..5376)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5402..5857)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5539..5560)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5751..5770
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   5929..6058
                     /label=AAV2 ITR
     promoter        join(6369..6561,1..85)
                     /label=chicken beta-actin promoter
ORIGIN
        1 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta
       61 taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg
      121 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
      181 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
      241 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg
      301 ggggagcggc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
      361 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
      421 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
      481 ttggcaaaga attggatccg aattcactag tctcgaggat atcgccacca tgccagagcc
      541 agcgaagtct gctcccgccc cgaaaaaggg ctccaagaag gcggtgacta aggcgcagaa
      601 gaaaggcggc aagaagcgca agcgcagccg caaggagagc tattccatct atgtgtacaa
      661 ggttctgaag caggtccacc ctgacaccgg catttcgtcc aaggccatgg gcatcatgaa
      721 ttcgtttgtg aacgacattt tcgagcgcat cgcaggtgag gcttcccgcc tggcgcatta
      781 caacaagcgc tcgaccatca cctccaggga gatccagacg gccgtgcgcc tgctgctgcc
      841 tggggagttg gccaagcacg ccgtgtccga gggtactaag gccatcacca agtacaccag
      901 cgctaaggat ccaccggtcg ccaccatggt gagcaagggc gaggaggtca tcaaagagtt
      961 catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg
     1021 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1081 cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc
     1141 gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt
     1201 caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc
     1261 ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc
     1321 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
     1381 cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg
     1441 ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg
     1501 ctactactac gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt
     1561 ggaacagtac gagcgctccg agggccgcca ccacctgttc ctggggcatg gcaccggcag
     1621 caccggcagc ggcagctccg gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa
     1681 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
     1741 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     1801 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
     1861 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
     1921 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
     1981 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     2041 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     2101 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
     2161 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     2221 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     2281 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga
     2341 cgagctgtac aagtaataaa agcttatcga taatcaacct ctggattaca aaatttgtga
     2401 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     2461 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     2521 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     2581 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     2641 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     2701 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     2761 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     2821 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     2881 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     2941 cctttgggcc gcctccccgc atcgataccg tcgacccggg cggccgcttc gagcagacat
     3001 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     3061 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     3121 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
     3181 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc ttcctagagc
     3241 atggctacgt agataagtag catggcgggt taatcattaa ctacaaggaa cccctagtga
     3301 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     3361 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgca
     3421 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3481 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3541 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3601 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3661 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3721 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3781 tccgactctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3841 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3901 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3961 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4021 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4081 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4141 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4201 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4261 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4321 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4381 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4441 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4501 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4561 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4621 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4681 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4741 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4801 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4861 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4921 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4981 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     5041 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     5101 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     5161 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     5221 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     5281 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5341 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5401 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     5461 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     5521 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     5581 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     5641 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     5701 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     5761 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     5821 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
     5881 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct gcgcgctcgc
     5941 tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc
     6001 tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag gggttccttg
     6061 tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag gaagagtacc
     6121 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     6181 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     6241 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     6301 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     6361 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     6421 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     6481 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
     6541 gagaggtgcg gcggcagcca a
//
