LOCUS       sequence-490080-        9843 bp    DNA     circular SYN 21-OCT-2025
DEFINITION  Expression of cofilin-mRFP to normal levels behind a cofilin 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9843)
  TITLE     Bamburg lab plasmids 2025
REFERENCE   2  (bases 1 to 9843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 21, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: huCof WTmRFP
FEATURES             Location/Qualifiers
     source          1..9843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     CDS             complement(629..640)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2165..2842
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             2165..2842
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(2295..2313)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2563..2582
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2757..2780
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     repeat_region   6802..6904
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(6993..7010)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7164..7752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7244..7263)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8584..9378
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8584..9378
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8638..8657)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9248..9267
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(9426..9806)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9488..9509)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9700..9719
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactggtacc gcttcggcct
      361 ccacctggag ctccagcagt gtagcccacg ggtgcagtca cctggagctc ccgcagtgta
      421 gcccacgggt gcagtctgac tacaatcccc tgactacaac ccccggcatc ctctgcgtgc
      481 tgcctcgctc ctggggaagc tcctctttta caccctttat tcagcttggg gaggtcaagg
      541 gccccggttt cctttgcctc tgagttgttt gcgactttgc cctttttttc taaggccatg
      601 gtcattgttt caggtcagag aaattctcct ggagttccca ccccagttta ttttaaactt
      661 ttgctttcat atagattctc ttcagtcttt gtgctaaaat gatcataaac ctatcaactc
      721 tggaattaaa ttgaagtatt ccttttcctg tatcgtaaaa gaaacataaa gaagtttttt
      781 ttttgttttg ttttgttttg ttttgttttt tgatttttca agacagggtt tctctgtgta
      841 gccctggctg tcctggaact cactttgtag accaggcttg cctcgcactc agaaatccgc
      901 ctgcctctgc ctcccgagtg ctgggattaa aggtgtgcgc caccatgccc ggccataagg
      961 aaatttttag tgcacaactc ttcacccttc ccccatgaat tacgtttgat ctaacaattt
     1021 gccaaacgtc tttagacaac gccaaagctc tcaaactttt tatgttagca tttcctgttt
     1081 tttaaaaaat cctatttgtt gaaattttac tccacgggag catttctatt ggcttgaaga
     1141 caccgttata cttggcgatt ctcttttctg tgagagagga tcccaagctg atgattcaaa
     1201 atcttttttc aggaaccata aagaacaaaa aaccacttaa tttctgggat aaagaaagta
     1261 gagaattata ttcaggtcat ttttatcaaa cgatgggcct ctggctcaga gcgttttttg
     1321 gtccattgca ccaatgggct cactctccgt gcaacagccc acgcctctcg gcctccgcct
     1381 actctgcgcc tcaagctctg cggaacccgg tagcagctag ttctatttcg gtgcaaaatc
     1441 ctcggacttc acttcccgtc ggcccgcgcg gcaagcgccg gaaggccgcc ccgcccctca
     1501 ttgtgcggct cctactaaac ggaaggggcc gggagaggcc gcgttcagtc ggatcccggc
     1561 agcagctgcg gcggctcagg tcttcagttg ctctttctgt ctcccggtac cgcggccgct
     1621 cgagcctaag cttgatatcg aattcaccac catggcctcc ggtgtggctg tctctgatgg
     1681 tgtcatcaag gtgttcaacg acatgaaggt gcgtaagagc tcaaccccag aggaggtgaa
     1741 gaagcgcaag aaggcggtgc tcttctgcct gagtgaggac aagaagaaca tcatcctgga
     1801 ggagggcaag gagatcctgg tgggcgatgt gggccagact gtcgacgatc cctacgccac
     1861 ctttgtcaag atgctgccag ataaggactg ccgctatgcc ctctatgatg caacctatga
     1921 gaccaaggag agcaagaagg aggatctggt gtttatcttc tgggcccccg agtctgcgcc
     1981 ccttaagagc aaaatgattt atgccagctc caaggacgcc atcaagaaga agctgacagg
     2041 gatcaagcat gaattgcaag caaactgcta cgaggaggtc aaggaccgct gcaccctggc
     2101 agagaagctg gggggcagtg cggtcatctc cctggagggc aagcctttgt tatcccggga
     2161 tccgatggcc tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga
     2221 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     2281 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     2341 catcctgtcc cctcagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat
     2401 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     2461 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     2521 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     2581 gaccatgggc tgggaggcct ccaccgagcg gatgtacccc gaggacggcg ccctgaaggg
     2641 cgagatcaag atgaggctga agctgaagga cggcggccac tacgacgccg aggtcaagac
     2701 cacctacatg gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg acatcaagct
     2761 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gcgccgaggg
     2821 ccgccactcc accggcgcct aagaattggc cgcgactcta gagatatcga attcaagctt
     2881 gtcgactcga agatctgccg tcatttccct ggagggcaag cctttgtgag ccacctccag
     2941 ccccctgcct ggagcatctt aacagcccca gacctgctct tgggtgttgc aggctgcccc
     3001 tttcctgcca gaccggaggg gctgggggga tcccagcagg gggagggcta tcccttcacc
     3061 ccagttgcca aacatccctc ccaccccctg gaccgtcctt ctccctccat ccctgacggt
     3121 tctggccttc ccaaactgct tttgatcttc tgattcctct tgggttgacg cagaccaagt
     3181 cccgtcctag gcacccagtt tggggggagc ctgtattttt ttttttaacg acacccctac
     3241 tccgtatccc tccccatccc atgctgccaa cttctaacca caatagtgac tctgtgcttg
     3301 tctgtttagt tctgtgtgta aatgaaatgt ggaaatgacc ctccctgccc cagctggctg
     3361 ccctcccctt tcctttgatc ttgaccactc atggaagcag gaccagtaag ggaccttcaa
     3421 tttaaaacaa aacaaaacaa aaaaacaata aaaaggctaa ttaacaacat ggcttttggg
     3481 tttgggattt tggggaccct gtggtgggag atggatgggt ggtttccctg tgcttcacct
     3541 ctagttcagt ccctagtaag cggcatctcc ttcttgagtt ggggtgcact gttccaaggc
     3601 agggcagttg ggctcagatc tgggcgtggt taagggtggg aaagaatata taaggtgggg
     3661 gtcttatgta gttttgtatc tgttttgcag cagccgccgc cgccatgagc accaactcgt
     3721 ttgatggaag cattgtgagc tcatatttga caacgcgcat gcccccatgg gccggggtgc
     3781 gtcagaatgt gatgggctcc agcattgatg gtcgccccgt cctgcccgca aactctacta
     3841 ccttgaccta cgagaccgtg tctggaacgc cgttggagac tgcagcctcc gccgccgctt
     3901 cagccgctgc agccaccgcc cgcgggattg tgactgactt tgctttcctg agcccgcttg
     3961 caagcagtgc agcttcccgt tcatccgccc gcgatgacaa gttgacggct cttttggcac
     4021 aattggattc tttgacccgg gaacttaatg tcgtttctca gcagctgttg gatctgcgcc
     4081 agcaggtttc tgccctgaag gcttcctccc ctcccaatgc ggtttaaaac ataaataaaa
     4141 aaccagactc tgtttggatt tggatcaagc aagtgtcttg ctgtctttat ttaggggttt
     4201 tgcgcgcgcg gtaggcccgg gaccagcggt ctcggtcgtt gagggtcctg tgtatttttt
     4261 ccaggacgtg gtaaaggtga ctctggatgt tcagatacat gggcataagc ccgtctctgg
     4321 ggtggaggta gcaccactgc agagcttcat gctgcggggt ggtgttgtag atgatccagt
     4381 cgtagcagga gcgctgggcg tggtgcctaa aaatgtcttt cagtagcaag ctgattgcca
     4441 ggggcaggcc cttggtgtaa gtgtttacaa agcggttaag ctgggatggg tgcatacgtg
     4501 gggatatgag atgcatcttg gactgtattt ttaggttggc tatgttccca gccatatccc
     4561 tccggggatt catgttgtgc agaaccacca gcacagtgta tccggtgcac ttgggaaatt
     4621 tgtcatgtag cttagaagga aatgcgtgga agaacttgga gacgcccttg tgacctccaa
     4681 gattttccat gcattcgtcc ataatgatgg caatgggccc acgggcggcg gcctgggcga
     4741 agatatttct gggatcacta acgtcatagt tgtgttccag gatgagatcg tcataggcca
     4801 tttttacaaa gcgcgggcgg agggtgccag actgcggtat aatggttcca tccggcccag
     4861 gggcgtagtt accctcacag atttgcattt cccacgcttt gagttcagat ggggggatca
     4921 tgtctacctg cggggcgatg aagaaaacgg tttccggggt aggggagatc agctgggaag
     4981 aaagcaggtt cctgagcagc tgcgacttac cgcagccggt gggcccgtaa atcacaccta
     5041 ttaccggctg caactggtag ttaagagagc tgcagctgcc gtcatccctg agcagggggg
     5101 ccacttcgtt aagcatgtcc ctgactcgca tgttttccct gaccaaatcc gccagaaggc
     5161 gctcgccgcc cagcgatagc agttcttgca aggaagcaaa gtttttcaac ggtttgagac
     5221 cgtccgccgt aggcatgctt ttgagcgttt gaccaagcag ttccaggcgg tcccacagct
     5281 cggtcacctg ctctacggca tctcgatcca gcatatctcc tcgtttcgcg ggttggggcg
     5341 gctttcgctg tacggcagta gtcggtgctc gtccagacgg gccagggtca tgtctttcca
     5401 cgggcgcagg gtcctcgtca gcgtagtctg ggtcacggtg aaggggtgcg ctccgggctg
     5461 cgcgctggcc agggtgcgct tgaggctggt cctgctggtg ctgaagcgct gccggtcttc
     5521 gccctgcgcg tcggccaggt agcatttgac catggtgtca tagtccagcc cctccgcggc
     5581 gtggcccttg gcgcgcagct tgcccttgga ggaggcgccg cacgaggggc agtgcagact
     5641 tttgagggcg tagagcttgg gcgcgagaaa taccgattcc ggggagtagg catccgcgcc
     5701 gcaggccccg cagacggtct cgcattccac gagccaggtg agctctggcc gttcggggtc
     5761 aaaaaccagg tttcccccat gctttttgat gcgtttctta cctctggttt ccatgagccg
     5821 gtgtccacgc tcggtgacga aaaggctgtc cgtgtccccg tatacagact tgagagggag
     5881 tttaaacgaa ttcaatagct tgttgcatgg gcggcgatat aaaatgcaag gtgctgctca
     5941 aaaaatcagg caaagcctcg cgcaaaaaag aaagcacatc gtagtcatgc tcatgcagat
     6001 aaaggcaggt aagctccgga accaccacag aaaaagacac catttttctc tcaaacatgt
     6061 ctgcgggttt ctgcataaac acaaaataaa ataacaaaaa aacatttaaa cattagaagc
     6121 ctgtcttaca acaggaaaaa caacccttat aagcataaga cggactacgg ccatgccggc
     6181 gtgaccgtaa aaaaactggt caccgtgatt aaaaagcacc accgacagct cctcggtcat
     6241 gtccggagtc ataatgtaag actcggtaaa cacatcaggt tgattcacat cggtcagtgc
     6301 taaaaagcga ccgaaatagc ccgggggaat acatacccgc aggcgtagag acaacattac
     6361 agcccccata ggaggtataa caaaattaat aggagagaaa aacacataaa cacctgaaaa
     6421 accctcctgc ctaggcaaaa tagcaccctc ccgctccaga acaacataca gcgcttccac
     6481 agcggcagcc ataacagtca gccttaccag taaaaaagaa aacctattaa aaaaacacca
     6541 ctcgacacgg caccagctca atcagtcaca gtgtaaaaaa gggccaagtg cagagcgagt
     6601 atatatagga ctaaaaaatg acgtaacggt taaagtccac aaaaaacacc cagaaaaccg
     6661 cacgcgaacc tacgcccaga aacgaaagcc aaaaaaccca caacttcctc aaatcgtcac
     6721 ttccgttttc ccacgttacg tcacttccca ttttaagaaa actacaattc ccaacacata
     6781 caagttactc cgccctaaaa cctacgtcac ccgccccgtt cccacgcccc gcgccacgtc
     6841 acaaactcca ccccctcatt atcatattgg cttcaatcca aaataaggta tattattgat
     6901 gatgttaatt aacatgcatg gatccatatg cggtgtgaaa taccgcacag atgcgtaagg
     6961 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7021 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7081 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7141 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7201 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7261 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7321 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7381 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7441 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7501 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7561 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     7621 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7681 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7741 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7801 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7861 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7921 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7981 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8041 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8101 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8161 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8221 aacgttgttg ccattgctgc agccatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8281 ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg
     8341 gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt
     8401 acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct
     8461 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg
     8521 ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt
     8581 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     8641 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     8701 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     8761 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     8821 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     8881 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     8941 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     9001 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     9061 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     9121 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     9181 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     9241 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     9301 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     9361 cttgacgagt tcttctgaat tttgttaaaa tttttgttaa atcagctcat tttttaacca
     9421 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     9481 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     9541 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     9601 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     9661 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     9721 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     9781 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggatcgaatt aattcttaat
     9841 taa
//
