LOCUS       sequence-490082-       13851 bp    DNA     circular SYN 21-OCT-2025
DEFINITION  EGFP fused at the N-terminus of tubulin; lentiviral construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13851)
  AUTHORS   Yang W, Guo X, Thein S, Xu F, Sugii S, Baas PW, Radda GK, Han W
  TITLE     Regulation of adipogenesis by cytoskeleton remodelling is 
            facilitated by acetyltransferase MEC-17-dependent acetylation of 
            alpha-tubulin.
  JOURNAL   Biochem J. 2013 Feb 1;449(3):605-12. doi: 10.1042/BJ20121121.
  PUBMED    23126280
REFERENCE   2  (bases 1 to 13851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 21, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2013-02-1- 1"; volume: "449"; issue: "3"; pages:
            "605-12"
COMMENT     Sequence Label: L304-EGFP-Tubulin-WT
FEATURES             Location/Qualifiers
     source          1..13851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..215
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     136..155
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        256..1467
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1304..1323
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1504..1513
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1510..1512,1513..1515,1516..2226)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1510..1512,1513..1515,1516..2226)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      3683..3692
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3689..3691,3692..3694,3695..4405)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3689..3691,3692..3694,3695..4405)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    5935..6523
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5988..6008)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6406..6417)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6526..6542)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6527..6543)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7052..7232
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7258..7275)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7264..7488
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7534..7962
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7621..7640)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7831..7852
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7971..7991)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7976..8305
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8156..8291
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8218..8237
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8353..8400
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8419..8793
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8923..9044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8960..8979)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9014..9033
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9093..9109)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9093..9109)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9106..9128)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9117..9133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9141..9147,9148..9165,9166..9171))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9186..9207
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9324..9341)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9495..10083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9575..9594)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10254..11045,11046..11114))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10877..10896
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11115..11219)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11294..11313)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11485..11864
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11866..12064
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12019..12039
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             12082..12262
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12309..12434
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12927..13160
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13345..13389
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13687..13804
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gaacgctgac gtcatcaacc cgctccaagg aatcgcgggc ccagtgtcac taggcgggaa
       61 cacccagcgc gcgtgcgccc tggcaggaag atggctgtga gggacagggg agtggcgccc
      121 tgcaatattt gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga aatgtctttg
      181 gatttgggaa tcttataagt tctgtatgag accacctcga gtctagagtt aacttaatta
      241 acccacccaa gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc
      301 tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc
      361 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
      421 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
      481 gcgaaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
      541 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
      601 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta
      661 gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc
      721 gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg
      781 gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
      841 tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
      901 aggtcttgag gccttcgcta atgcgggaaa gctcttactc gggtgagatg ggctggggca
      961 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
     1021 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
     1081 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
     1141 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
     1201 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
     1261 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
     1321 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
     1381 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
     1441 tggccgtttt tggctttttt gttagacgaa gcttggtacc gagcggatct agcgctaccg
     1501 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1561 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1621 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1681 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     1741 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1801 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1861 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1921 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     1981 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     2041 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2101 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2161 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2221 tacaagtccg gactcagatc tcgagtgcgt gagtgcatct ccatccacgt tggccaggct
     2281 ggtgtccaga ttggcaatgc ctgctgggag ctctactgcc tggaacacgg catccagccc
     2341 gatggccaga tgccaagtga caagaccatt gggggaggag atgactcctt caacaccttc
     2401 ttcagtgaga cgggcgctgg caagcacgtg ccccgggctg tgtttgtaga cttggaaccc
     2461 acagtcattg atgaagttcg cactggcacc taccgccagc tcttccaccc tgagcagctc
     2521 atcacaggca aggaagatgc tgccaataac tatgcccgag ggcactacac cattggcaag
     2581 gagatcattg accttgtgtt ggaccgaatt cgcaagctgg ctgaccagtg caccggtctt
     2641 cagggcttct tggttttcca cagctttggt gggggaactg gttctgggtt cacctccctg
     2701 ctcatggaac gtctctcagt tgattatggc aagaagtcca agctggagtt ctccatttac
     2761 ccagcacccc aggtttccac agctgtagtt gagccctaca actccatcct caccacccac
     2821 accaccctgg agcactctga ttgtgccttc atggtagaca atgaggccat ctatgacatc
     2881 tgtcgtagaa acctcgatat cgagcgccca acctacacta accttaaccg ccttattagc
     2941 cagattgtgt cctccatcac tgcttccctg agatttgatg gagccctgaa tgttgacctg
     3001 acagaattcc agaccaacct ggtgccctac ccccgcatcc acttccctct ggccacatat
     3061 gcccctgtca tctctgctga gaaagcctac catgaacagc tttctgtagc agagatcacc
     3121 aatgcttgct ttgagccagc caaccagatg gtgaaatgtg accctcgcca tggtaaatac
     3181 atggcttgct gcctgttgta ccgtggtgac gtggttccca aagatgtcaa tgctgccatt
     3241 gccaccatca aaaccaagcg cagcatccag tttgtggatt ggtgccccac tggcttcaag
     3301 gttggcatca actaccagcc tcccactgtg gtgcctggtg gagacctggc caaggtacag
     3361 agagctgtgt gcatgctgag caacaccaca gccattgctg aggcctgggc tcgcctggac
     3421 cacaagtttg acctgatgta tgccaagcgt gcctttgttc actggtacgt gggtgagggg
     3481 atggaggaag gcgagttttc agaggcccgt gaagatatgg ctgcccttga gaaggattat
     3541 gaggaggttg gtgtggattc tgttgaagga gagggtgagg aagaaggaga ggaatactaa
     3601 ggatccacta gtccagtgtg gtggaattct gcagatatcc agcacagtgg cggccgctcg
     3661 agtctagcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     3721 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3781 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     3841 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     3901 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     3961 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     4021 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     4081 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     4141 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     4201 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     4261 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     4321 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     4381 tctcggcatg gacgagctgt acaagtccgg actcagatct cgagtgcgtg agtgcatctc
     4441 catccacgtt ggccaggctg gtgtccagat tggcaatgcc tgctgggagc tctactgcct
     4501 ggaacacggc atccagcccg atggccagat gccaagtgac aagaccattg ggggaggaga
     4561 tgactccttc aacaccttct tcagtgagac gggcgctggc aagcacgtgc cccgggctgt
     4621 gtttgtagac ttggaaccca cagtcattga tgaagttcgc actggcacct accgccagct
     4681 cttccaccct gagcagctca tcacaggcaa ggaagatgct gccaataact atgcccgagg
     4741 gcactacacc attggcaagg agatcattga ccttgtgttg gaccgaattc gcaagctggc
     4801 tgaccagtgc accggtcttc agggcttctt ggttttccac agctttggtg ggggaactgg
     4861 ttctgggttc acctccctgc tcatggaacg tctctcagtt gattatggca agaagtccaa
     4921 gctggagttc tccatttacc cagcacccca ggtttccaca gctgtagttg agccctacaa
     4981 ctccatcctc accacccaca ccaccctgga gcactctgat tgtgccttca tggtagacaa
     5041 tgaggccatc tatgacatct gtcgtagaaa cctcgatatc gagcgcccaa cctacactaa
     5101 ccttaaccgc cttattagcc agattgtgtc ctccatcact gcttccctga gatttgatgg
     5161 agccctgaat gttgacctga cagaattcca gaccaacctg gtgccctacc cccgcatcca
     5221 cttccctctg gccacatatg cccctgtcat ctctgctgag aaagcctacc atgaacagct
     5281 ttctgtagca gagatcacca atgcttgctt tgagccagcc aaccagatgg tgaaatgtga
     5341 ccctcgccat ggtaaataca tggcttgctg cctgttgtac cgtggtgacg tggttcccaa
     5401 agatgtcaat gctgccattg ccaccatcaa aaccaagcgc agcatccagt ttgtggattg
     5461 gtgccccact ggcttcaagg ttggcatcaa ctaccagcct cccactgtgg tgcctggtgg
     5521 agacctggcc aaggtacaga gagctgtgtg catgctgagc aacaccacag ccattgctga
     5581 ggcctgggct cgcctggacc acaagtttga cctgatgtat gccaagcgtg cctttgttca
     5641 ctggtacgtg ggtgagggga tggaggaagg cgagttttca gaggcccgtg aagatatggc
     5701 tgcccttgag aaggattatg aggaggttgg tgtggattct gttgaaggag agggtgagga
     5761 agaaggagag gaatactaag gatccactag tccagtgtgg tggaattctg cagatatcca
     5821 gcacagtggc ggccgctcga gtctaggatc cgaattcgct agcccacgcg cgtggtgtac
     5881 aagtaaagcg gccgcgactc tagtccctcc caattcgata tcaagcttat cgataatcaa
     5941 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6001 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6061 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6121 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6181 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6241 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6301 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6361 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6421 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6481 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     6541 cgatcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     6601 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     6661 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6721 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     6781 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     6841 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     6901 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     6961 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     7021 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     7081 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7141 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7201 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     7261 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     7321 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     7381 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     7441 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     7501 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     7561 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     7621 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7681 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7741 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7801 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7861 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     7921 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     7981 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8041 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8101 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8161 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8221 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     8281 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     8341 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     8401 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     8461 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     8521 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     8581 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     8641 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     8701 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     8761 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     8821 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     8881 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     8941 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9001 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     9061 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     9121 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     9181 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     9241 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     9301 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9361 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9421 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9481 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9541 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9601 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9661 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9721 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9781 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9841 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9901 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9961 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10021 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10081 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10141 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    10201 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10261 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10321 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10381 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10441 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10501 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    10561 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    10621 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10681 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10741 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10801 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10861 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10921 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10981 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    11041 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    11101 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    11161 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    11221 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
    11281 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
    11341 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
    11401 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
    11461 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
    11521 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
    11581 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
    11641 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
    11701 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
    11761 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
    11821 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
    11881 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
    11941 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
    12001 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
    12061 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
    12121 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
    12181 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
    12241 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
    12301 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
    12361 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
    12421 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
    12481 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
    12541 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
    12601 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
    12661 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
    12721 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
    12781 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
    12841 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
    12901 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
    12961 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
    13021 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
    13081 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
    13141 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
    13201 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
    13261 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
    13321 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
    13381 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
    13441 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
    13501 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
    13561 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
    13621 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
    13681 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
    13741 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
    13801 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattctag c
//
