LOCUS       sequence-490093-       10211 bp    DNA     circular SYN 21-OCT-2025
DEFINITION  Silencing of endogenous cofilin in rat/mouse cells with replacement 
            by human cofilin K22Q expressed with a cofilin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10211)
  TITLE     Bamburg lab plasmids 2025
REFERENCE   2  (bases 1 to 10211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 21, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MCP-huCof K22Q + rat cof shRNA
FEATURES             Location/Qualifiers
     source          1..10211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     CDS             complement(629..640)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2165..2842
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             2165..2842
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(2295..2313)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2563..2582
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2757..2780
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        3674..3888
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     3809..3828
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     repeat_region   7170..7272
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(7361..7378)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7532..8120)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7612..7631)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8952..9746
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8952..9746
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9006..9025)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9616..9635
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(9794..10174)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9856..9877)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10068..10087
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactggtacc gcttcggcct
      361 ccacctggag ctccagcagt gtagcccacg ggtgcagtca cctggagctc ccgcagtgta
      421 gcccacgggt gcagtctgac tacaatcccc tgactacaac ccccggcatc ctctgcgtgc
      481 tgcctcgctc ctggggaagc tcctctttta caccctttat tcagcttggg gaggtcaagg
      541 gccccggttt cctttgcctc tgagttgttt gcgactttgc cctttttttc taaggccatg
      601 gtcattgttt caggtcagag aaattctcct ggagttccca ccccagttta ttttaaactt
      661 ttgctttcat atagattctc ttcagtcttt gtgctaaaat gatcataaac ctatcaactc
      721 tggaattaaa ttgaagtatt ccttttcctg tatcgtaaaa gaaacataaa gaagtttttt
      781 ttttgttttg ttttgttttg ttttgttttt tgatttttca agacagggtt tctctgtgta
      841 gccctggctg tcctggaact cactttgtag accaggcttg cctcgcactc agaaatccgc
      901 ctgcctctgc ctcccgagtg ctgggattaa aggtgtgcgc caccatgccc ggccataagg
      961 aaatttttag tgcacaactc ttcacccttc ccccatgaat tacgtttgat ctaacaattt
     1021 gccaaacgtc tttagacaac gccaaagctc tcaaactttt tatgttagca tttcctgttt
     1081 tttaaaaaat cctatttgtt gaaattttac tccacgggag catttctatt ggcttgaaga
     1141 caccgttata cttggcgatt ctcttttctg tgagagagga tcccaagctg atgattcaaa
     1201 atcttttttc aggaaccata aagaacaaaa aaccacttaa tttctgggat aaagaaagta
     1261 gagaattata ttcaggtcat ttttatcaaa cgatgggcct ctggctcaga gcgttttttg
     1321 gtccattgca ccaatgggct cactctccgt gcaacagccc acgcctctcg gcctccgcct
     1381 actctgcgcc tcaagctctg cggaacccgg tagcagctag ttctatttcg gtgcaaaatc
     1441 ctcggacttc acttcccgtc ggcccgcgcg gcaagcgccg gaaggccgcc ccgcccctca
     1501 ttgtgcggct cctactaaac ggaaggggcc gggagaggcc gcgttcagtc ggatcccggc
     1561 agcagctgcg gcggctcagg tcttcagttg ctctttctgt ctcccggtac cgcggccgct
     1621 cgagcctaag cttgatatcg aattcaccac catggcctcc ggtgtggctg tctctgatgg
     1681 tgtcatcaag gtgttcaacg acatgaaggt gcgtcagagc tcaaccccag aggaggtgaa
     1741 gaagcgcaag aaggcggtgc tcttctgcct gagtgaggac aagaagaaca tcatcctgga
     1801 ggagggcaag gagatcctgg tgggcgatgt gggccagact gtcgacgatc cctacgccac
     1861 ctttgtcaag atgctgccag ataaggactg ccgctatgcc ctctatgatg caacctatga
     1921 gaccaaggag agcaagaagg aggatctggt gtttatcttc tgggcccccg agtctgcgcc
     1981 ccttaagagc aaaatgattt atgccagctc caaggacgcc atcaagaaga agctgacagg
     2041 gatcaagcat gaattgcaag caaactgcta cgaggaggtc aaggaccgct gcaccctggc
     2101 agagaagctg gggggcagtg cggtcatctc cctggagggc aagcctttgt tatcccggga
     2161 tccgatggcc tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga
     2221 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     2281 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     2341 catcctgtcc cctcagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat
     2401 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     2461 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     2521 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     2581 gaccatgggc tgggaggcct ccaccgagcg gatgtacccc gaggacggcg ccctgaaggg
     2641 cgagatcaag atgaggctga agctgaagga cggcggccac tacgacgccg aggtcaagac
     2701 cacctacatg gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg acatcaagct
     2761 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gcgccgaggg
     2821 ccgccactcc accggcgcct aagaattggc cgcgactcta gagatatcga attcaagctt
     2881 gtcgactcga agatcgatct gccgtcattt ccctggaggg caagcctttg tgagccacct
     2941 ccagccccct gcctggagca tcttaacagc cccagacctg ctcttgggtg ttgcaggctg
     3001 cccctttcct gccagaccgg aggggctggg gggatcccag cagggggagg gctatccctt
     3061 caccccagtt gccaaacatc cctcccaccc cctggaccgt ccttctccct ccatccctga
     3121 cggttctggc cttcccaaac tgcttttgat cttctgattc ctcttgggtt gacgcagacc
     3181 aagtcccgtc ctaggcaccc agtttggggg gagcctgtat tttttttttt aacgacaccc
     3241 ctactccgta tccctcccca tcccatgctg ccaacttcta accacaatag tgactctgtg
     3301 cttgtctgtt tagttctgtg tgtaaatgaa atgtggaaat gaccctccct gccccagctg
     3361 gctgccctcc cctttccttt gatcttgacc actcatggaa gcaggaccag taagggacct
     3421 tcaatttaaa acaaaacaaa acaaaaaaac aataaaaagg ctaattaaca acatggcttt
     3481 tgggtttggg attttgggga ccctgtggtg ggagatggat gggtggtttc cctgtgcttc
     3541 acctctagtt cagtccctag taagcggcat ctccttcttg agttggggtg cactgttcca
     3601 aggcagggca gttgggctca gatccactag taacggccgc cagtgtgctg gaattcgccc
     3661 ttccatggaa ttcgaacgct gacgtcatca acccgctcca aggaatcgcg ggcccagtgt
     3721 cactaggcgg gaacacccag cgcgcgtgcg ccctggcagg aagatggctg tgagggacag
     3781 gggagtggcg ccctgcaata tttgcatgtc gctatgtgtt ctgggaaatc accataaacg
     3841 tgaaatgtct ttggatttgg gaatcttata agttctgtat gagaccacag atctaaggtg
     3901 ttcaatgaca tgaaattctc gagatttcat gtcattgaac accttttttt ggaaagcttg
     3961 atatcgaatt cctgcagccc gggggatctg ggcgtggtta agggtgggaa agaatatata
     4021 aggtgggggt cttatgtagt tttgtatctg ttttgcagca gccgccgccg ccatgagcac
     4081 caactcgttt gatggaagca ttgtgagctc atatttgaca acgcgcatgc ccccatgggc
     4141 cggggtgcgt cagaatgtga tgggctccag cattgatggt cgccccgtcc tgcccgcaaa
     4201 ctctactacc ttgacctacg agaccgtgtc tggaacgccg ttggagactg cagcctccgc
     4261 cgccgcttca gccgctgcag ccaccgcccg cgggattgtg actgactttg ctttcctgag
     4321 cccgcttgca agcagtgcag cttcccgttc atccgcccgc gatgacaagt tgacggctct
     4381 tttggcacaa ttggattctt tgacccggga acttaatgtc gtttctcagc agctgttgga
     4441 tctgcgccag caggtttctg ccctgaaggc ttcctcccct cccaatgcgg tttaaaacat
     4501 aaataaaaaa ccagactctg tttggatttg gatcaagcaa gtgtcttgct gtctttattt
     4561 aggggttttg cgcgcgcggt aggcccggga ccagcggtct cggtcgttga gggtcctgtg
     4621 tattttttcc aggacgtggt aaaggtgact ctggatgttc agatacatgg gcataagccc
     4681 gtctctgggg tggaggtagc accactgcag agcttcatgc tgcggggtgg tgttgtagat
     4741 gatccagtcg tagcaggagc gctgggcgtg gtgcctaaaa atgtctttca gtagcaagct
     4801 gattgccagg ggcaggccct tggtgtaagt gtttacaaag cggttaagct gggatgggtg
     4861 catacgtggg gatatgagat gcatcttgga ctgtattttt aggttggcta tgttcccagc
     4921 catatccctc cggggattca tgttgtgcag aaccaccagc acagtgtatc cggtgcactt
     4981 gggaaatttg tcatgtagct tagaaggaaa tgcgtggaag aacttggaga cgcccttgtg
     5041 acctccaaga ttttccatgc attcgtccat aatgatggca atgggcccac gggcggcggc
     5101 ctgggcgaag atatttctgg gatcactaac gtcatagttg tgttccagga tgagatcgtc
     5161 ataggccatt tttacaaagc gcgggcggag ggtgccagac tgcggtataa tggttccatc
     5221 cggcccaggg gcgtagttac cctcacagat ttgcatttcc cacgctttga gttcagatgg
     5281 ggggatcatg tctacctgcg gggcgatgaa gaaaacggtt tccggggtag gggagatcag
     5341 ctgggaagaa agcaggttcc tgagcagctg cgacttaccg cagccggtgg gcccgtaaat
     5401 cacacctatt accggctgca actggtagtt aagagagctg cagctgccgt catccctgag
     5461 caggggggcc acttcgttaa gcatgtccct gactcgcatg ttttccctga ccaaatccgc
     5521 cagaaggcgc tcgccgccca gcgatagcag ttcttgcaag gaagcaaagt ttttcaacgg
     5581 tttgagaccg tccgccgtag gcatgctttt gagcgtttga ccaagcagtt ccaggcggtc
     5641 ccacagctcg gtcacctgct ctacggcatc tcgatccagc atatctcctc gtttcgcggg
     5701 ttggggcggc tttcgctgta cggcagtagt cggtgctcgt ccagacgggc cagggtcatg
     5761 tctttccacg ggcgcagggt cctcgtcagc gtagtctggg tcacggtgaa ggggtgcgct
     5821 ccgggctgcg cgctggccag ggtgcgcttg aggctggtcc tgctggtgct gaagcgctgc
     5881 cggtcttcgc cctgcgcgtc ggccaggtag catttgacca tggtgtcata gtccagcccc
     5941 tccgcggcgt ggcccttggc gcgcagcttg cccttggagg aggcgccgca cgaggggcag
     6001 tgcagacttt tgagggcgta gagcttgggc gcgagaaata ccgattccgg ggagtaggca
     6061 tccgcgccgc aggccccgca gacggtctcg cattccacga gccaggtgag ctctggccgt
     6121 tcggggtcaa aaaccaggtt tcccccatgc tttttgatgc gtttcttacc tctggtttcc
     6181 atgagccggt gtccacgctc ggtgacgaaa aggctgtccg tgtccccgta tacagacttg
     6241 agagggagtt taaacgaatt caatagcttg ttgcatgggc ggcgatataa aatgcaaggt
     6301 gctgctcaaa aaatcaggca aagcctcgcg caaaaaagaa agcacatcgt agtcatgctc
     6361 atgcagataa aggcaggtaa gctccggaac caccacagaa aaagacacca tttttctctc
     6421 aaacatgtct gcgggtttct gcataaacac aaaataaaat aacaaaaaaa catttaaaca
     6481 ttagaagcct gtcttacaac aggaaaaaca acccttataa gcataagacg gactacggcc
     6541 atgccggcgt gaccgtaaaa aaactggtca ccgtgattaa aaagcaccac cgacagctcc
     6601 tcggtcatgt ccggagtcat aatgtaagac tcggtaaaca catcaggttg attcacatcg
     6661 gtcagtgcta aaaagcgacc gaaatagccc gggggaatac atacccgcag gcgtagagac
     6721 aacattacag cccccatagg aggtataaca aaattaatag gagagaaaaa cacataaaca
     6781 cctgaaaaac cctcctgcct aggcaaaata gcaccctccc gctccagaac aacatacagc
     6841 gcttccacag cggcagccat aacagtcagc cttaccagta aaaaagaaaa cctattaaaa
     6901 aaacaccact cgacacggca ccagctcaat cagtcacagt gtaaaaaagg gccaagtgca
     6961 gagcgagtat atataggact aaaaaatgac gtaacggtta aagtccacaa aaaacaccca
     7021 gaaaaccgca cgcgaaccta cgcccagaaa cgaaagccaa aaaacccaca acttcctcaa
     7081 atcgtcactt ccgttttccc acgttacgtc acttcccatt ttaagaaaac tacaattccc
     7141 aacacataca agttactccg ccctaaaacc tacgtcaccc gccccgttcc cacgccccgc
     7201 gccacgtcac aaactccacc ccctcattat catattggct tcaatccaaa ataaggtata
     7261 ttattgatga tgttaattaa catgcatgga tccatatgcg gtgtgaaata ccgcacagat
     7321 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     7381 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7441 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7501 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7561 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7621 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7681 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7741 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7801 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7861 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7921 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     7981 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8041 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8101 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8161 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8221 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8281 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8341 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8401 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8461 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8521 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8581 gtttgcgcaa cgttgttgcc attgctgcag ccatgagatt atcaaaaagg atcttcacct
     8641 agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca
     8701 gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca
     8761 gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat
     8821 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     8881 tcttgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag
     8941 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     9001 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     9061 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     9121 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     9181 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     9241 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     9301 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     9361 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     9421 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     9481 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     9541 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     9601 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     9661 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     9721 atcgccttct tgacgagttc ttctgaattt tgttaaaatt tttgttaaat cagctcattt
     9781 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     9841 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     9901 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     9961 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
    10021 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
    10081 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
    10141 cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg atcgaattaa
    10201 ttcttaatta a
//
