LOCUS       sequence-490100-       10194 bp    DNA     circular SYN 21-OCT-2025
DEFINITION  Silencing of endogenous cofilin in rat/mouse cells with replacement 
            by an inactive human cofilin expressed with a cofilin promoter 
            (control for plasmid listed above).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10194)
  TITLE     Bamburg lab plasmids 2025
REFERENCE   2  (bases 1 to 10194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 21, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: huCof null + rat cof shRNA
FEATURES             Location/Qualifiers
     source          1..10194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     CDS             complement(629..640)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2148..2825
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             2148..2825
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(2278..2296)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2546..2565
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2740..2763
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        3657..3871
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     3792..3811
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     repeat_region   7153..7255
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(7344..7361)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7515..8103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7595..7614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8935..9729
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8935..9729
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8989..9008)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9599..9618
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(9777..10157)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9839..9860)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10051..10070
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactggtacc gcttcggcct
      361 ccacctggag ctccagcagt gtagcccacg ggtgcagtca cctggagctc ccgcagtgta
      421 gcccacgggt gcagtctgac tacaatcccc tgactacaac ccccggcatc ctctgcgtgc
      481 tgcctcgctc ctggggaagc tcctctttta caccctttat tcagcttggg gaggtcaagg
      541 gccccggttt cctttgcctc tgagttgttt gcgactttgc cctttttttc taaggccatg
      601 gtcattgttt caggtcagag aaattctcct ggagttccca ccccagttta ttttaaactt
      661 ttgctttcat atagattctc ttcagtcttt gtgctaaaat gatcataaac ctatcaactc
      721 tggaattaaa ttgaagtatt ccttttcctg tatcgtaaaa gaaacataaa gaagtttttt
      781 ttttgttttg ttttgttttg ttttgttttt tgatttttca agacagggtt tctctgtgta
      841 gccctggctg tcctggaact cactttgtag accaggcttg cctcgcactc agaaatccgc
      901 ctgcctctgc ctcccgagtg ctgggattaa aggtgtgcgc caccatgccc ggccataagg
      961 aaatttttag tgcacaactc ttcacccttc ccccatgaat tacgtttgat ctaacaattt
     1021 gccaaacgtc tttagacaac gccaaagctc tcaaactttt tatgttagca tttcctgttt
     1081 tttaaaaaat cctatttgtt gaaattttac tccacgggag catttctatt ggcttgaaga
     1141 caccgttata cttggcgatt ctcttttctg tgagagagga tcccaagctg atgattcaaa
     1201 atcttttttc aggaaccata aagaacaaaa aaccacttaa tttctgggat aaagaaagta
     1261 gagaattata ttcaggtcat ttttatcaaa cgatgggcct ctggctcaga gcgttttttg
     1321 gtccattgca ccaatgggct cactctccgt gcaacagccc acgcctctcg gcctccgcct
     1381 actctgcgcc tcaagctctg cggaacccgg tagcagctag ttctatttcg gtgcaaaatc
     1441 ctcggacttc acttcccgtc ggcccgcgcg gcaagcgccg gaaggccgcc ccgcccctca
     1501 ttgtgcggct cctactaaac ggaaggggcc gggagaggcc gcgttcagtc ggatcccggc
     1561 agcagctgcg gcggctcagg tcttcagttg ctctttctgt ctcccggtac cgcggccgct
     1621 cgagcctaag cttcgaattc accgccatgg ctgtctctga tggtgtcatc aaggtgttca
     1681 acgacatgaa ggtgcgtaag agctcaaccc cagaggaggt gaagaagcgc aagaaggcgg
     1741 tgctcttctg cctgagtgag gacaagaaga acatcatcct ggaggagggc aaggagatcc
     1801 tggtgggcga tgtgggccag actgtcgacg atccctacgc cacctttgtc aagatgctgc
     1861 cagataagga ctgccgcttt gccctctatg atgcaaccta tgagaccaag gagagccagc
     1921 aggaggatct ggtgtttatc ttctgggccc ccgagtctgc gccccttaag agcaaaatga
     1981 tttatgccag ctccaaggac gccatcaaga agaagctgac agggatcaag catgaattgc
     2041 aagcaaactg ctacgaggag gtcaaggacc gctgcaccct ggcagagaag ctggggggca
     2101 gtgcggtcat ctccctggag ggcaagcctt tgttatcccg ggatccgatg gcctcctccg
     2161 aggacgtcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc
     2221 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     2281 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     2341 tccagtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     2401 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     2461 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     2521 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     2581 cctccaccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagatgaggc
     2641 tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga
     2701 agcccgtgca gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca
     2761 acgaggacta caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg
     2821 cctaagaatt ggccgcgact ctagagatat cgaattcaag cttgtcgact cgaagatcga
     2881 tctgccgtca tttccctgga gggcaagcct ttgtgagcca cctccagccc cctgcctgga
     2941 gcatcttaac agccccagac ctgctcttgg gtgttgcagg ctgccccttt cctgccagac
     3001 cggaggggct ggggggatcc cagcaggggg agggctatcc cttcacccca gttgccaaac
     3061 atccctccca ccccctggac cgtccttctc cctccatccc tgacggttct ggccttccca
     3121 aactgctttt gatcttctga ttcctcttgg gttgacgcag accaagtccc gtcctaggca
     3181 cccagtttgg ggggagcctg tatttttttt tttaacgaca cccctactcc gtatccctcc
     3241 ccatcccatg ctgccaactt ctaaccacaa tagtgactct gtgcttgtct gtttagttct
     3301 gtgtgtaaat gaaatgtgga aatgaccctc cctgccccag ctggctgccc tcccctttcc
     3361 tttgatcttg accactcatg gaagcaggac cagtaaggga ccttcaattt aaaacaaaac
     3421 aaaacaaaaa aacaataaaa aggctaatta acaacatggc ttttgggttt gggattttgg
     3481 ggaccctgtg gtgggagatg gatgggtggt ttccctgtgc ttcacctcta gttcagtccc
     3541 tagtaagcgg catctccttc ttgagttggg gtgcactgtt ccaaggcagg gcagttgggc
     3601 tcagatccac tagtaacggc cgccagtgtg ctggaattcg cccttccatg gaattcgaac
     3661 gctgacgtca tcaacccgct ccaaggaatc gcgggcccag tgtcactagg cgggaacacc
     3721 cagcgcgcgt gcgccctggc aggaagatgg ctgtgaggga caggggagtg gcgccctgca
     3781 atatttgcat gtcgctatgt gttctgggaa atcaccataa acgtgaaatg tctttggatt
     3841 tgggaatctt ataagttctg tatgagacca cagatctaag gtgttcaatg acatgaaatt
     3901 ctcgagattt catgtcattg aacacctttt tttggaaagc ttgatatcga attcctgcag
     3961 cccgggggat ctgggcgtgg ttaagggtgg gaaagaatat ataaggtggg ggtcttatgt
     4021 agttttgtat ctgttttgca gcagccgccg ccgccatgag caccaactcg tttgatggaa
     4081 gcattgtgag ctcatatttg acaacgcgca tgcccccatg ggccggggtg cgtcagaatg
     4141 tgatgggctc cagcattgat ggtcgccccg tcctgcccgc aaactctact accttgacct
     4201 acgagaccgt gtctggaacg ccgttggaga ctgcagcctc cgccgccgct tcagccgctg
     4261 cagccaccgc ccgcgggatt gtgactgact ttgctttcct gagcccgctt gcaagcagtg
     4321 cagcttcccg ttcatccgcc cgcgatgaca agttgacggc tcttttggca caattggatt
     4381 ctttgacccg ggaacttaat gtcgtttctc agcagctgtt ggatctgcgc cagcaggttt
     4441 ctgccctgaa ggcttcctcc cctcccaatg cggtttaaaa cataaataaa aaaccagact
     4501 ctgtttggat ttggatcaag caagtgtctt gctgtcttta tttaggggtt ttgcgcgcgc
     4561 ggtaggcccg ggaccagcgg tctcggtcgt tgagggtcct gtgtattttt tccaggacgt
     4621 ggtaaaggtg actctggatg ttcagataca tgggcataag cccgtctctg gggtggaggt
     4681 agcaccactg cagagcttca tgctgcgggg tggtgttgta gatgatccag tcgtagcagg
     4741 agcgctgggc gtggtgccta aaaatgtctt tcagtagcaa gctgattgcc aggggcaggc
     4801 ccttggtgta agtgtttaca aagcggttaa gctgggatgg gtgcatacgt ggggatatga
     4861 gatgcatctt ggactgtatt tttaggttgg ctatgttccc agccatatcc ctccggggat
     4921 tcatgttgtg cagaaccacc agcacagtgt atccggtgca cttgggaaat ttgtcatgta
     4981 gcttagaagg aaatgcgtgg aagaacttgg agacgccctt gtgacctcca agattttcca
     5041 tgcattcgtc cataatgatg gcaatgggcc cacgggcggc ggcctgggcg aagatatttc
     5101 tgggatcact aacgtcatag ttgtgttcca ggatgagatc gtcataggcc atttttacaa
     5161 agcgcgggcg gagggtgcca gactgcggta taatggttcc atccggccca ggggcgtagt
     5221 taccctcaca gatttgcatt tcccacgctt tgagttcaga tggggggatc atgtctacct
     5281 gcggggcgat gaagaaaacg gtttccgggg taggggagat cagctgggaa gaaagcaggt
     5341 tcctgagcag ctgcgactta ccgcagccgg tgggcccgta aatcacacct attaccggct
     5401 gcaactggta gttaagagag ctgcagctgc cgtcatccct gagcaggggg gccacttcgt
     5461 taagcatgtc cctgactcgc atgttttccc tgaccaaatc cgccagaagg cgctcgccgc
     5521 ccagcgatag cagttcttgc aaggaagcaa agtttttcaa cggtttgaga ccgtccgccg
     5581 taggcatgct tttgagcgtt tgaccaagca gttccaggcg gtcccacagc tcggtcacct
     5641 gctctacggc atctcgatcc agcatatctc ctcgtttcgc gggttggggc ggctttcgct
     5701 gtacggcagt agtcggtgct cgtccagacg ggccagggtc atgtctttcc acgggcgcag
     5761 ggtcctcgtc agcgtagtct gggtcacggt gaaggggtgc gctccgggct gcgcgctggc
     5821 cagggtgcgc ttgaggctgg tcctgctggt gctgaagcgc tgccggtctt cgccctgcgc
     5881 gtcggccagg tagcatttga ccatggtgtc atagtccagc ccctccgcgg cgtggccctt
     5941 ggcgcgcagc ttgcccttgg aggaggcgcc gcacgagggg cagtgcagac ttttgagggc
     6001 gtagagcttg ggcgcgagaa ataccgattc cggggagtag gcatccgcgc cgcaggcccc
     6061 gcagacggtc tcgcattcca cgagccaggt gagctctggc cgttcggggt caaaaaccag
     6121 gtttccccca tgctttttga tgcgtttctt acctctggtt tccatgagcc ggtgtccacg
     6181 ctcggtgacg aaaaggctgt ccgtgtcccc gtatacagac ttgagaggga gtttaaacga
     6241 attcaatagc ttgttgcatg ggcggcgata taaaatgcaa ggtgctgctc aaaaaatcag
     6301 gcaaagcctc gcgcaaaaaa gaaagcacat cgtagtcatg ctcatgcaga taaaggcagg
     6361 taagctccgg aaccaccaca gaaaaagaca ccatttttct ctcaaacatg tctgcgggtt
     6421 tctgcataaa cacaaaataa aataacaaaa aaacatttaa acattagaag cctgtcttac
     6481 aacaggaaaa acaaccctta taagcataag acggactacg gccatgccgg cgtgaccgta
     6541 aaaaaactgg tcaccgtgat taaaaagcac caccgacagc tcctcggtca tgtccggagt
     6601 cataatgtaa gactcggtaa acacatcagg ttgattcaca tcggtcagtg ctaaaaagcg
     6661 accgaaatag cccgggggaa tacatacccg caggcgtaga gacaacatta cagcccccat
     6721 aggaggtata acaaaattaa taggagagaa aaacacataa acacctgaaa aaccctcctg
     6781 cctaggcaaa atagcaccct cccgctccag aacaacatac agcgcttcca cagcggcagc
     6841 cataacagtc agccttacca gtaaaaaaga aaacctatta aaaaaacacc actcgacacg
     6901 gcaccagctc aatcagtcac agtgtaaaaa agggccaagt gcagagcgag tatatatagg
     6961 actaaaaaat gacgtaacgg ttaaagtcca caaaaaacac ccagaaaacc gcacgcgaac
     7021 ctacgcccag aaacgaaagc caaaaaaccc acaacttcct caaatcgtca cttccgtttt
     7081 cccacgttac gtcacttccc attttaagaa aactacaatt cccaacacat acaagttact
     7141 ccgccctaaa acctacgtca cccgccccgt tcccacgccc cgcgccacgt cacaaactcc
     7201 accccctcat tatcatattg gcttcaatcc aaaataaggt atattattga tgatgttaat
     7261 taacatgcat ggatccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     7321 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7381 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7441 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7501 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7561 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7621 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7681 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7741 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7801 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7861 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7921 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     7981 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8041 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8101 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8161 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8221 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8281 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8341 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8401 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8461 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8521 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8581 gccattgctg cagccatgag attatcaaaa aggatcttca cctagatcct tttcacgtag
     8641 aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg
     8701 acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga
     8761 tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc
     8821 tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc
     8881 tgatggcgca ggggatcaag ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     8941 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     9001 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     9061 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     9121 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     9181 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     9241 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     9301 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     9361 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     9421 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     9481 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     9541 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     9601 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     9661 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     9721 ttcttctgaa ttttgttaaa atttttgtta aatcagctca ttttttaacc aataggccga
     9781 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     9841 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     9901 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     9961 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
    10021 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
    10081 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
    10141 gccgctacag ggcgcgtcca ttcgccattc aggatcgaat taattcttaa ttaa
//
