LOCUS       sequence-490153-        9709 bp    DNA     circular SYN 22-OCT-2025
DEFINITION  Expression of WT coifilin resistant to shRNA in cells coexpressing 
            mRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9709)
  TITLE     Bamburg lab plasmids 2025
REFERENCE   2  (bases 1 to 9709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 22, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: huCof WT SSR RedTrack
FEATURES             Location/Qualifiers
     source          1..9709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(22..402)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(84..105)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     296..315
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   440..542
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     630..780
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     polyA_signal    923..1044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1192..1869)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(1192..1869)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(1254..1277)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1452..1471)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1721..1739
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(1912..2115)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        2116..2419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    2505..2626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3341..3544)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        3545..3848
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   7107..7209
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(7298..7315)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7469..8057)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7549..7568)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8889..9683
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8889..9683
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8943..8962)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9553..9572
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
       61 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      121 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      181 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      241 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      301 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      361 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggat
      421 cgaattaatt cttaattaac atcatcaata atatacctta ttttggattg aagccaatat
      481 gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt
      541 agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa cacatgtaag cgacggatgt
      601 ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg
      661 ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt aagatttggc cattttcgcg
      721 ggaaaactga ataagaggaa gtgaaatctg aataattttg tgttactcat agcgcgtaat
      781 atttgtctag ggccgcgggg actttgaccg tttacgtgga gactcgccca ggtgtttttc
      841 tcaggtgttt tccgcgttcc gggtcaaagt tggcgtttta ttattatagt cagtcgagtc
      901 tagcaagcta gcttgatcgc gttaagatac attgatgagt ttggacaaac cacaactaga
      961 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     1021 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     1081 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     1141 gattatgatc ttgtggtatg gctgattatg atctagagtc gcggccaatt cttaggcgcc
     1201 ggtggagtgg cggccctcgg cgcgctcgta ctgttccacg atggtgtagt cctcgttgtg
     1261 ggaggtgatg tccagcttga tgtcggtctt gtaggcgccg ggcagctgca cgggcttctt
     1321 ggccatgtag gtggtcttga cctcggcgtc gtagtggccg ccgtccttca gcttcagcct
     1381 catcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg tggaggcctc
     1441 ccagcccatg gtcttcttct gcattacggg gccgtcggag gggaagttgg tgccgcgcag
     1501 cttcaccttg tagatgaact cgccgtcctg cagggaggag tcctgggtca cggtcaccac
     1561 gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt
     1621 caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt actggaactg
     1681 aggggacagg atgtcccagg cgaagggcag ggggccgccc ttggtcacct tcagcttggc
     1741 ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc
     1801 gttcacggag ccctccatgc gcaccttgaa gcgcatgaac tccttgatga cgtcctcgga
     1861 ggaggccatc ggatcccggt agcgctagcg gatctgacgg ttcactaaac cagctctgct
     1921 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
     1981 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
     2041 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
     2101 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     2161 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     2221 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     2281 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     2341 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     2401 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     2461 tgattactat taatcactag cagatcgtcg ataaacctcc gcgttaagat acattgatga
     2521 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2581 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2641 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2701 cctctacaaa tgtggtatgg ctgattatga tcagttatct agatccggtg gatcggatat
     2761 cttatctaga agcttgatat cctcacaaag gcttgccctc cagggagatg accgcactgc
     2821 cccccagctt ctctgccagg gtgcagcggt ccttgacctc ctcgtagcag tttgcttgca
     2881 attcatgctt gatccctgtc agcttcttct tgatggcgtc cttggagctg gcataaatca
     2941 ttttgctctt aaggggcgca gactcggggg cccagaagat aaacaccaga tcctccttct
     3001 tgctctcctt ggtctcatag gttgcatcat agagggcata gcggcagtcc ttatctggca
     3061 gcattttcac aaacgtcgcg tagggatcgt cgacagtctg gcccacatcg cccaccagga
     3121 tctccttgcc ctcctccagg atgatgttct tcttgtcctc actcaggcag aagagcaccg
     3181 ccttcttgcg cttcttcacc tcctctgggg ttgagctctt acgcaccttc atgtcgttga
     3241 acaccttgat gacaccatca gagacagcca caccggaggc catggtggtg aattcctgca
     3301 gcccggggga tctctagcgg atctgacggt tcactaaacc agctctgctt atatagacct
     3361 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
     3421 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
     3481 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
     3541 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
     3601 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     3661 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
     3721 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
     3781 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
     3841 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
     3901 agacgtttat cactgctaga ggatctgggc gtggttaagg gtgggaaaga atatataagg
     3961 tgggggtctt atgtagtttt gtatctgttt tgcagcagcc gccgccgcca tgagcaccaa
     4021 ctcgtttgat ggaagcattg tgagctcata tttgacaacg cgcatgcccc catgggccgg
     4081 ggtgcgtcag aatgtgatgg gctccagcat tgatggtcgc cccgtcctgc ccgcaaactc
     4141 tactaccttg acctacgaga ccgtgtctgg aacgccgttg gagactgcag cctccgccgc
     4201 cgcttcagcc gctgcagcca ccgcccgcgg gattgtgact gactttgctt tcctgagccc
     4261 gcttgcaagc agtgcagctt cccgttcatc cgcccgcgat gacaagttga cggctctttt
     4321 ggcacaattg gattctttga cccgggaact taatgtcgtt tctcagcagc tgttggatct
     4381 gcgccagcag gtttctgccc tgaaggcttc ctcccctccc aatgcggttt aaaacataaa
     4441 taaaaaacca gactctgttt ggatttggat caagcaagtg tcttgctgtc tttatttagg
     4501 ggttttgcgc gcgcggtagg cccgggacca gcggtctcgg tcgttgaggg tcctgtgtat
     4561 tttttccagg acgtggtaaa ggtgactctg gatgttcaga tacatgggca taagcccgtc
     4621 tctggggtgg aggtagcacc actgcagagc ttcatgctgc ggggtggtgt tgtagatgat
     4681 ccagtcgtag caggagcgct gggcgtggtg cctaaaaatg tctttcagta gcaagctgat
     4741 tgccaggggc aggcccttgg tgtaagtgtt tacaaagcgg ttaagctggg atgggtgcat
     4801 acgtggggat atgagatgca tcttggactg tatttttagg ttggctatgt tcccagccat
     4861 atccctccgg ggattcatgt tgtgcagaac caccagcaca gtgtatccgg tgcacttggg
     4921 aaatttgtca tgtagcttag aaggaaatgc gtggaagaac ttggagacgc ccttgtgacc
     4981 tccaagattt tccatgcatt cgtccataat gatggcaatg ggcccacggg cggcggcctg
     5041 ggcgaagata tttctgggat cactaacgtc atagttgtgt tccaggatga gatcgtcata
     5101 ggccattttt acaaagcgcg ggcggagggt gccagactgc ggtataatgg ttccatccgg
     5161 cccaggggcg tagttaccct cacagatttg catttcccac gctttgagtt cagatggggg
     5221 gatcatgtct acctgcgggg cgatgaagaa aacggtttcc ggggtagggg agatcagctg
     5281 ggaagaaagc aggttcctga gcagctgcga cttaccgcag ccggtgggcc cgtaaatcac
     5341 acctattacc ggctgcaact ggtagttaag agagctgcag ctgccgtcat ccctgagcag
     5401 gggggccact tcgttaagca tgtccctgac tcgcatgttt tccctgacca aatccgccag
     5461 aaggcgctcg ccgcccagcg atagcagttc ttgcaaggaa gcaaagtttt tcaacggttt
     5521 gagaccgtcc gccgtaggca tgcttttgag cgtttgacca agcagttcca ggcggtccca
     5581 cagctcggtc acctgctcta cggcatctcg atccagcata tctcctcgtt tcgcgggttg
     5641 gggcggcttt cgctgtacgg cagtagtcgg tgctcgtcca gacgggccag ggtcatgtct
     5701 ttccacgggc gcagggtcct cgtcagcgta gtctgggtca cggtgaaggg gtgcgctccg
     5761 ggctgcgcgc tggccagggt gcgcttgagg ctggtcctgc tggtgctgaa gcgctgccgg
     5821 tcttcgccct gcgcgtcggc caggtagcat ttgaccatgg tgtcatagtc cagcccctcc
     5881 gcggcgtggc ccttggcgcg cagcttgccc ttggaggagg cgccgcacga ggggcagtgc
     5941 agacttttga gggcgtagag cttgggcgcg agaaataccg attccgggga gtaggcatcc
     6001 gcgccgcagg ccccgcagac ggtctcgcat tccacgagcc aggtgagctc tggccgttcg
     6061 gggtcaaaaa ccaggtttcc cccatgcttt ttgatgcgtt tcttacctct ggtttccatg
     6121 agccggtgtc cacgctcggt gacgaaaagg ctgtccgtgt ccccgtatac agacttgaga
     6181 gggagtttaa acgaattcaa tagcttgttg catgggcggc gatataaaat gcaaggtgct
     6241 gctcaaaaaa tcaggcaaag cctcgcgcaa aaaagaaagc acatcgtagt catgctcatg
     6301 cagataaagg caggtaagct ccggaaccac cacagaaaaa gacaccattt ttctctcaaa
     6361 catgtctgcg ggtttctgca taaacacaaa ataaaataac aaaaaaacat ttaaacatta
     6421 gaagcctgtc ttacaacagg aaaaacaacc cttataagca taagacggac tacggccatg
     6481 ccggcgtgac cgtaaaaaaa ctggtcaccg tgattaaaaa gcaccaccga cagctcctcg
     6541 gtcatgtccg gagtcataat gtaagactcg gtaaacacat caggttgatt cacatcggtc
     6601 agtgctaaaa agcgaccgaa atagcccggg ggaatacata cccgcaggcg tagagacaac
     6661 attacagccc ccataggagg tataacaaaa ttaataggag agaaaaacac ataaacacct
     6721 gaaaaaccct cctgcctagg caaaatagca ccctcccgct ccagaacaac atacagcgct
     6781 tccacagcgg cagccataac agtcagcctt accagtaaaa aagaaaacct attaaaaaaa
     6841 caccactcga cacggcacca gctcaatcag tcacagtgta aaaaagggcc aagtgcagag
     6901 cgagtatata taggactaaa aaatgacgta acggttaaag tccacaaaaa acacccagaa
     6961 aaccgcacgc gaacctacgc ccagaaacga aagccaaaaa acccacaact tcctcaaatc
     7021 gtcacttccg ttttcccacg ttacgtcact tcccatttta agaaaactac aattcccaac
     7081 acatacaagt tactccgccc taaaacctac gtcacccgcc ccgttcccac gccccgcgcc
     7141 acgtcacaaa ctccaccccc tcattatcat attggcttca atccaaaata aggtatatta
     7201 ttgatgatgt taattaacat gcatggatcc atatgcggtg tgaaataccg cacagatgcg
     7261 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7321 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7381 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7441 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7501 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7561 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7621 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7681 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7741 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7801 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7861 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7921 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7981 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8041 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8101 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8161 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8221 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8281 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8341 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8401 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8461 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8521 tgcgcaacgt tgttgccatt gctgcagcca tgagattatc aaaaaggatc ttcacctaga
     8581 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct
     8641 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg
     8701 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc
     8761 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
     8821 tgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat
     8881 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     8941 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     9001 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     9061 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     9121 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     9181 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     9241 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     9301 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     9361 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     9421 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     9481 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     9541 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     9601 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     9661 gccttcttga cgagttcttc tgaattttgt taaaattttt gttaaatca
//
