LOCUS       sequence-490156-        9133 bp    DNA     circular SYN 22-OCT-2025
DEFINITION  Expression of ADF behind a mouse cofilin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9133)
  TITLE     Bamburg lab plasmids 2025
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 22, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MCP-huADF WT
FEATURES             Location/Qualifiers
     source          1..9133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     CDS             complement(629..640)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     repeat_region   6092..6194
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(6283..6300)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6454..7042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6534..6553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             7874..8668
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7874..8668
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7928..7947)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8538..8557
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(8716..9096)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8778..8799)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8990..9009
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactggtacc gcttcggcct
      361 ccacctggag ctccagcagt gtagcccacg ggtgcagtca cctggagctc ccgcagtgta
      421 gcccacgggt gcagtctgac tacaatcccc tgactacaac ccccggcatc ctctgcgtgc
      481 tgcctcgctc ctggggaagc tcctctttta caccctttat tcagcttggg gaggtcaagg
      541 gccccggttt cctttgcctc tgagttgttt gcgactttgc cctttttttc taaggccatg
      601 gtcattgttt caggtcagag aaattctcct ggagttccca ccccagttta ttttaaactt
      661 ttgctttcat atagattctc ttcagtcttt gtgctaaaat gatcataaac ctatcaactc
      721 tggaattaaa ttgaagtatt ccttttcctg tatcgtaaaa gaaacataaa gaagtttttt
      781 ttttgttttg ttttgttttg ttttgttttt tgatttttca agacagggtt tctctgtgta
      841 gccctggctg tcctggaact cactttgtag accaggcttg cctcgcactc agaaatccgc
      901 ctgcctctgc ctcccgagtg ctgggattaa aggtgtgcgc caccatgccc ggccataagg
      961 aaatttttag tgcacaactc ttcacccttc ccccatgaat tacgtttgat ctaacaattt
     1021 gccaaacgtc tttagacaac gccaaagctc tcaaactttt tatgttagca tttcctgttt
     1081 tttaaaaaat cctatttgtt gaaattttac tccacgggag catttctatt ggcttgaaga
     1141 caccgttata cttggcgatt ctcttttctg tgagagagga tcccaagctg atgattcaaa
     1201 atcttttttc aggaaccata aagaacaaaa aaccacttaa tttctgggat aaagaaagta
     1261 gagaattata ttcaggtcat ttttatcaaa cgatgggcct ctggctcaga gcgttttttg
     1321 gtccattgca ccaatgggct cactctccgt gcaacagccc acgcctctcg gcctccgcct
     1381 actctgcgcc tcaagctctg cggaacccgg tagcagctag ttctatttcg gtgcaaaatc
     1441 ctcggacttc acttcccgtc ggcccgcgcg gcaagcgccg gaaggccgcc ccgcccctca
     1501 ttgtgcggct cctactaaac ggaaggggcc gggagaggcc gcgttcagtc ggatcccggc
     1561 agcagctgcg gcggctcagg tcttcagttg ctctttctgt ctcccggtac cgcggccgcc
     1621 tcgagtctag agatatctgc agaattcacc accatggcct caggagtgca agtagctgat
     1681 gaagtatgtc gcatttttta tgacatgaaa gttcgtaaat gctccacacc agaagaaatc
     1741 aagaaaagaa agaaggctgt cattttttgt ctcagtgcag acaaaaagtg catcattgta
     1801 gaagaaggca aagagatctt ggttggagat gttggtgtaa ccataactga tcctttcaag
     1861 cattttgtgg gaatgcttcc tgaaaaagat tgtcgctatg ctttgtatga tgcaagcttt
     1921 gaaacaaaag aatccagaaa agaagagttg atgttttttt tgtgggcacc agaactagca
     1981 cctctgaaaa gtaaaatgat ctatgcaagc tccaaggatg caattaaaaa gaaatttcaa
     2041 ggcataaaac atgaatgtca agcaaatgga ccagaagatc tcaatcgggc ttgtattgct
     2101 gaaaagttag gtggatcctt aattgtagcc tttgaaggat gccctgtgta gagatatcga
     2161 attcaagctt gtcgactcga agatctgccg tcatttccct ggagggcaag cctttgtgag
     2221 ccacctccag ccccctgcct ggagcatctt aacagcccca gacctgctct tgggtgttgc
     2281 aggctgcccc tttcctgcca gaccggaggg gctgggggga tcccagcagg gggagggcta
     2341 tcccttcacc ccagttgcca aacatccctc ccaccccctg gaccgtcctt ctccctccat
     2401 ccctgacggt tctggccttc ccaaactgct tttgatcttc tgattcctct tgggttgacg
     2461 cagaccaagt cccgtcctag gcacccagtt tggggggagc ctgtattttt ttttttaacg
     2521 acacccctac tccgtatccc tccccatccc atgctgccaa cttctaacca caatagtgac
     2581 tctgtgcttg tctgtttagt tctgtgtgta aatgaaatgt ggaaatgacc ctccctgccc
     2641 cagctggctg ccctcccctt tcctttgatc ttgaccactc atggaagcag gaccagtaag
     2701 ggaccttcaa tttaaaacaa aacaaaacaa aaaaacaata aaaaggctaa ttaacaacat
     2761 ggcttttggg tttgggattt tggggaccct gtggtgggag atggatgggt ggtttccctg
     2821 tgcttcacct ctagttcagt ccctagtaag cggcatctcc ttcttgagtt ggggtgcact
     2881 gttccaaggc agggcagttg ggctcagatc tgggcgtggt taagggtggg aaagaatata
     2941 taaggtgggg gtcttatgta gttttgtatc tgttttgcag cagccgccgc cgccatgagc
     3001 accaactcgt ttgatggaag cattgtgagc tcatatttga caacgcgcat gcccccatgg
     3061 gccggggtgc gtcagaatgt gatgggctcc agcattgatg gtcgccccgt cctgcccgca
     3121 aactctacta ccttgaccta cgagaccgtg tctggaacgc cgttggagac tgcagcctcc
     3181 gccgccgctt cagccgctgc agccaccgcc cgcgggattg tgactgactt tgctttcctg
     3241 agcccgcttg caagcagtgc agcttcccgt tcatccgccc gcgatgacaa gttgacggct
     3301 cttttggcac aattggattc tttgacccgg gaacttaatg tcgtttctca gcagctgttg
     3361 gatctgcgcc agcaggtttc tgccctgaag gcttcctccc ctcccaatgc ggtttaaaac
     3421 ataaataaaa aaccagactc tgtttggatt tggatcaagc aagtgtcttg ctgtctttat
     3481 ttaggggttt tgcgcgcgcg gtaggcccgg gaccagcggt ctcggtcgtt gagggtcctg
     3541 tgtatttttt ccaggacgtg gtaaaggtga ctctggatgt tcagatacat gggcataagc
     3601 ccgtctctgg ggtggaggta gcaccactgc agagcttcat gctgcggggt ggtgttgtag
     3661 atgatccagt cgtagcagga gcgctgggcg tggtgcctaa aaatgtcttt cagtagcaag
     3721 ctgattgcca ggggcaggcc cttggtgtaa gtgtttacaa agcggttaag ctgggatggg
     3781 tgcatacgtg gggatatgag atgcatcttg gactgtattt ttaggttggc tatgttccca
     3841 gccatatccc tccggggatt catgttgtgc agaaccacca gcacagtgta tccggtgcac
     3901 ttgggaaatt tgtcatgtag cttagaagga aatgcgtgga agaacttgga gacgcccttg
     3961 tgacctccaa gattttccat gcattcgtcc ataatgatgg caatgggccc acgggcggcg
     4021 gcctgggcga agatatttct gggatcacta acgtcatagt tgtgttccag gatgagatcg
     4081 tcataggcca tttttacaaa gcgcgggcgg agggtgccag actgcggtat aatggttcca
     4141 tccggcccag gggcgtagtt accctcacag atttgcattt cccacgcttt gagttcagat
     4201 ggggggatca tgtctacctg cggggcgatg aagaaaacgg tttccggggt aggggagatc
     4261 agctgggaag aaagcaggtt cctgagcagc tgcgacttac cgcagccggt gggcccgtaa
     4321 atcacaccta ttaccggctg caactggtag ttaagagagc tgcagctgcc gtcatccctg
     4381 agcagggggg ccacttcgtt aagcatgtcc ctgactcgca tgttttccct gaccaaatcc
     4441 gccagaaggc gctcgccgcc cagcgatagc agttcttgca aggaagcaaa gtttttcaac
     4501 ggtttgagac cgtccgccgt aggcatgctt ttgagcgttt gaccaagcag ttccaggcgg
     4561 tcccacagct cggtcacctg ctctacggca tctcgatcca gcatatctcc tcgtttcgcg
     4621 ggttggggcg gctttcgctg tacggcagta gtcggtgctc gtccagacgg gccagggtca
     4681 tgtctttcca cgggcgcagg gtcctcgtca gcgtagtctg ggtcacggtg aaggggtgcg
     4741 ctccgggctg cgcgctggcc agggtgcgct tgaggctggt cctgctggtg ctgaagcgct
     4801 gccggtcttc gccctgcgcg tcggccaggt agcatttgac catggtgtca tagtccagcc
     4861 cctccgcggc gtggcccttg gcgcgcagct tgcccttgga ggaggcgccg cacgaggggc
     4921 agtgcagact tttgagggcg tagagcttgg gcgcgagaaa taccgattcc ggggagtagg
     4981 catccgcgcc gcaggccccg cagacggtct cgcattccac gagccaggtg agctctggcc
     5041 gttcggggtc aaaaaccagg tttcccccat gctttttgat gcgtttctta cctctggttt
     5101 ccatgagccg gtgtccacgc tcggtgacga aaaggctgtc cgtgtccccg tatacagact
     5161 tgagagggag tttaaacgaa ttcaatagct tgttgcatgg gcggcgatat aaaatgcaag
     5221 gtgctgctca aaaaatcagg caaagcctcg cgcaaaaaag aaagcacatc gtagtcatgc
     5281 tcatgcagat aaaggcaggt aagctccgga accaccacag aaaaagacac catttttctc
     5341 tcaaacatgt ctgcgggttt ctgcataaac acaaaataaa ataacaaaaa aacatttaaa
     5401 cattagaagc ctgtcttaca acaggaaaaa caacccttat aagcataaga cggactacgg
     5461 ccatgccggc gtgaccgtaa aaaaactggt caccgtgatt aaaaagcacc accgacagct
     5521 cctcggtcat gtccggagtc ataatgtaag actcggtaaa cacatcaggt tgattcacat
     5581 cggtcagtgc taaaaagcga ccgaaatagc ccgggggaat acatacccgc aggcgtagag
     5641 acaacattac agcccccata ggaggtataa caaaattaat aggagagaaa aacacataaa
     5701 cacctgaaaa accctcctgc ctaggcaaaa tagcaccctc ccgctccaga acaacataca
     5761 gcgcttccac agcggcagcc ataacagtca gccttaccag taaaaaagaa aacctattaa
     5821 aaaaacacca ctcgacacgg caccagctca atcagtcaca gtgtaaaaaa gggccaagtg
     5881 cagagcgagt atatatagga ctaaaaaatg acgtaacggt taaagtccac aaaaaacacc
     5941 cagaaaaccg cacgcgaacc tacgcccaga aacgaaagcc aaaaaaccca caacttcctc
     6001 aaatcgtcac ttccgttttc ccacgttacg tcacttccca ttttaagaaa actacaattc
     6061 ccaacacata caagttactc cgccctaaaa cctacgtcac ccgccccgtt cccacgcccc
     6121 gcgccacgtc acaaactcca ccccctcatt atcatattgg cttcaatcca aaataaggta
     6181 tattattgat gatgttaatt aacatgcatg gatccatatg cggtgtgaaa taccgcacag
     6241 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     6301 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     6901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7321 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7501 tagtttgcgc aacgttgttg ccattgctgc agccatgaga ttatcaaaaa ggatcttcac
     7561 ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
     7621 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
     7681 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
     7741 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
     7801 tttcttgccg ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg
     7861 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     7921 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     7981 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     8041 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     8101 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     8161 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     8221 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     8281 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     8341 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     8401 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     8461 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     8521 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     8581 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     8641 ctatcgcctt cttgacgagt tcttctgaat tttgttaaaa tttttgttaa atcagctcat
     8701 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     8761 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     8821 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     8881 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     8941 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     9001 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     9061 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggatcgaatt
     9121 aattcttaat taa
//
