LOCUS       sequence-490162-        5549 bp    DNA     circular SYN 22-OCT-2025
DEFINITION  pTwist CMV BetaGlobin encoding magrolimab light chain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Stark JC, Gray MA, Wisnovsky S, Ibarlucea-Benitez I, Lustig M, Riley
            NM, Ribi MK, Errington WJ, Bruncsics B, Sarkar CA, Valerius TV, 
            Ravetch JV, Bertozzi CR
  TITLE     Antibody-lectin chimeras for glyco-immune checkpoint blockade
  JOURNAL   bioRxiv 2022.10.26.513931; doi: 
            https://doi.org/10.1101/2022.10.26.513931
REFERENCE   2  (bases 1 to 5549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 22, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.10.26.513931; doi: https://doi.org/10.1101/2022.10.26.513931"
COMMENT     Sequence Label: pTCB-magroLC
FEATURES             Location/Qualifiers
     source          1..5549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    134..205
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(431..1019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(511..530)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(1765..1833,1834..2625)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1983..2002)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      2638..2774
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2926..2997
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3002..3048)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3066..3114
                     /note="synthetic polyadenylation signal"
     enhancer        3195..3498
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3500..3702
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3653..3673
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          3836..4311
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     polyA_signal    5190..5311
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5201..5220)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5255..5274
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      5426..5472
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      join(5502..5549,1..89)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 tcccacatat gccagattca gcaacggata cggcttcccc aacttgccca cttccatacg
       61 tgtcctcctt accagaaatt tatccttaag gtcgtcagct atcctgcagg cgatctctcg
      121 atttcgatca agacattcct ttaatggtct tttctggaca ccactagggg tcagaagtag
      181 ttcatcaaac tttcttccct ccctaatctc attggttacc ttgggctatc gaaacttaat
      241 taaccagtca agtcagctac ttggcgagat cgacttgtct gggtttcgac tacgctcaga
      301 attgcgtcag tcaagttcga tctggtcctt gctattgcac ccgttctccg attacgagtt
      361 tcatttaaat catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      421 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      481 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      541 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      601 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      661 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
      721 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
      781 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
      841 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
      901 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
      961 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1021 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1081 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1141 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1201 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcattta
     1261 aatttccgaa ctctccaagg ccctcgtcgg aaaatcttca aacctttcgt ccgatccatc
     1321 ttgcaggcta cctctcgaac gaactatcgc aagtctcttg gccggccttg cgccttggct
     1381 attgcttggc agcgcctatc gccaggtatt actccaatcc cgaatatccg agatcgggat
     1441 cacccgagag aagttcaacc tacatcctca atcccgatct atccgagatc cgaggaatat
     1501 cgaaatcggg gcgcgcctgg tgtaccgaga acgatcctct cagtgcgagt ctcgacgatc
     1561 catatcgttg cttggcagtc agccagtcgg aatccagctt gggacccagg aagtccaatc
     1621 gtcagatatt gtactcaagc ctggtcacgg cagcgtaccg atctgtttaa acctagatat
     1681 tgatagtctg atcggtcaac gtataatcga gtcctagctt ttgcaaacat ctatcaagag
     1741 acaggatcag caggaggctt tcgcatgagt attcaacatt tccgtgtcgc ccttattccc
     1801 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1861 gatgctgaag atcagttggg tgcgcgagtg ggttacatcg aactggatct caacagcggt
     1921 aagatccttg agagttttcg ccccgaagaa cgctttccaa tgatgagcac ttttaaagtt
     1981 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     2041 atacactatt ctcagaatga cttggttgag tattcaccag tcacagaaaa gcatcttacg
     2101 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2161 gccaacttac ttctgacaac gattggagga ccgaaggagc taaccgcttt tttgcacaac
     2221 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2281 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caaccttgcg taaactatta
     2341 actggcgaac tacttactct agcttcccgg caacagttga tagactggat ggaggcggat
     2401 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2461 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2521 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2581 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaaccgat tctaggtgca
     2641 ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct tatactccca catatgccag
     2701 attcagcaac ggatacggct tccccaactt gcccacttcc atacgtgtcc tccttaccag
     2761 aaatttatcc ttaagatccc gaatcgttta aactcgactc tggctctatc gaatctccgt
     2821 cgtttcgagc ttacgcgaac agccgtggcg ctcatttgct cgtcgggcat cgaatctcgt
     2881 cagctatcgt cagcttacct ttttggcagc gatcgcggct cccgacatct tggaccatta
     2941 gctccacagg tatcttcttc cctctagtgg tcataacagc agcttcagct acctctcaat
     3001 tcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctatc aatcgttgcg
     3061 ttacacacac aaaaaaccaa cacacatcca tcttcgatgg atagcgattt tattatctaa
     3121 ctgctgatcg agtgtagcca gatctagtaa tcaattacgg ggtcattagt tcatagccca
     3181 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     3241 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     3301 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     3361 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3421 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3481 gtcatcgcta ttaccatgct gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3541 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3601 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3661 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     3721 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
     3781 agcctcccct cgaagcttac atgtggtacc gagctcggat cctgagaact tcagggtgag
     3841 tctatgggac ccttgatgtt ttctttcccc ttcttttcta tggttaagtt catgtcatag
     3901 gaaggggaga agtaacaggg tacacatatt gaccaaatca gggtaatttt gcatttgtaa
     3961 ttttaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt ctaatacttt
     4021 ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct ttgcaccatt
     4081 ctaaagaata acagtgataa tttctgggtt aaggcaatag caatatttct gcatataaat
     4141 atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata gcagctacaa
     4201 tccagctacc attctgcttt tattttatgg ttgggataag gctggattat tctgagtcca
     4261 agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca gctcctgggc
     4321 aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag gcggccgctg ccaagcttcc
     4381 gagctctcga attcaaagga ggtaccgcga tatctacctc gagatgggat ggtcatgtat
     4441 catccttttt ctagtagcaa ctgcaaccgg tgtacattcc gatgtgttga tgacgcaaac
     4501 cccattgtct ctcccagtaa gcctgggcga ccaagcgagt atctcttgcc gctcatccca
     4561 atcaatcgtt tattccaatg gcaacacata tctgggatgg tacctccaaa aaccagggca
     4621 aagcccaaag ctgcttatat ataaagtgtc aaatcgattt agcggagtcc cggacagatt
     4681 ttcagggtca gggtccggaa cagattttac gttgaaaatc tctcgcgtag aagcagagga
     4741 cctgggggtc tatcattgct ttcagggtag tcatgtccca tatacctttg gcggtggtac
     4801 aaaggtagaa atcaagcgca ccgtcgccgc gcccagcgtg ttcatcttcc cgcccagcga
     4861 cgagcagctg aagagcggca cggccagcgt ggtctgcctg ctcaacaact tctacccccg
     4921 ggaggccaag gtgcagtgga aggtggacaa cgccctgcag tcggggaaca gccaggagag
     4981 cgtcaccgag caggacagca aggacagcac ctacagcctg tcgagcaccc tcacgctgag
     5041 caaggccgac tacgagaagc acaaggtgta cgcgtgcgag gtgacccacc agggcctgag
     5101 cagccccgtc accaagtcgt tcaaccgcgg agaatgctga gctagcttga ctgactgaga
     5161 tacagcgtac cttcagctca cagacatgat aagatacatt gatgagtttg gacaaaccac
     5221 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     5281 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     5341 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     5401 tattggccca tctctatcgg tatcgtagca taaccccttg gggcctctaa acgggtcttg
     5461 aggggttttt tgtgcccctc gggccggatt gctatctacc ggcattggcg cagaaaaaaa
     5521 tgcctgatgc gacgctgcgc gtcttatac
//
