LOCUS       sequence-490231-        9283 bp    DNA     circular SYN 22-OCT-2025
DEFINITION  Neuronal specific expression of cof-mRFP .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Flynn KC, Pak CW, Shaw AE, Bradke F, Bamburg JR
  TITLE     Growth cone-like waves transport actin and promote axonogenesis and 
            neurite branching.
  JOURNAL   Dev Neurobiol. 2009 Oct;69(12):761-79. doi: 10.1002/dneu.20734.
  PUBMED    19513994
REFERENCE   2  (bases 1 to 9283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 22, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dneu"; 
            journalName: "Dev Neurobiol"; date: "2009-10"; volume: "69"; issue: 
            "12"; pages: "761-79"
COMMENT     Sequence Label: huCof WT-mRFP
FEATURES             Location/Qualifiers
     source          1..9283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   51..153
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     241..391
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     CDS             2137..2814
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             2137..2814
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(2267..2285)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2535..2554
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2729..2752
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    2974..3095
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3011..3030)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3065..3084
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6292..6394
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(6483..6500)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6654..7242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6734..6753)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8074..8868
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8074..8868
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8128..8147)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8738..8757
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(join(8916..9283,1..13))
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8978..8999)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9190..9209
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa catcatcaat
       61 aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt ttgtgacgtg
      121 gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt gatgttgcaa
      181 gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg gtgtgcgccg
      241 gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag taaatttggg
      301 cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga agtgaaatct
      361 gaataatttt gtgttactca tagcgcgtaa tactgtaatc ttaaggggac agtaaaggtg
      421 atggcaggaa ggcagccccc ggaggtcaaa ggctgggcac gcgggaggag aggccagagt
      481 cagaggctgc gggtatctca gatatgaagg aaagatgaga gaggctcagg aagaggtaag
      541 aaaagacaca agagaccaga gaagggagaa gaattagaga gggaggcaga ggaccgctgt
      601 ctctacagac atagctggta gagactggga ggaagggatg aaccctgagc gcatgaaggg
      661 aaggaggtgg ctggtggtat atggaggatg tagctgggcc agggaaaaga tcctgcacta
      721 aaaatctgaa gctaaaaata acaggacacg gggtggagag gcgaaaggag ggcagattga
      781 ggcagagaga ctgagaggcc tggggatgtg ggcattccgg tagggcacac agttcacttg
      841 tcttctcttt ttccaggagg ccaaagatgc tgacctcaag aactcataat accccagtgg
      901 ggaccaccgc attcatagcc ctgttacaag aagtgggaga tgttcctttt tgtcccagac
      961 tggaaatcca ttacatcccg aggctcaggt tctgtggtgg tcatctctgt gtggcttgtt
     1021 ctgtgggcct acctaaagtc ctaagcacag ctctcaagca gatccgaggc gactaagatg
     1081 ctagtagggg ttgtctggag agaagagccg aggaggtggg ctgtgatgga tcagttcagc
     1141 tttcaaataa aaaggcgttt ttatattctg tgtcgagttc gtgaacccct gtggtgggct
     1201 tctccatctg tctgggttag tacctgccac tatactggaa taaggggacg cctgcttccc
     1261 tcgagttggc tggacaaggt tatgagcatc cgtgtactta tggggttgcc agcttggtcc
     1321 tggatcgccc gggcccttcc cccacccgtt cggttcccca ccaccacccg cgctcgtacg
     1381 tgcgtctccg cctgcagctc ttgactcatc ggggcccccg ggtcacatgc gctcgctcgg
     1441 ctctataggc gccgccccct gcccaccccc cgcccgcgct gggagccgca gccgccgcca
     1501 ctcctgctct ctctgcgccg ccgccgtcac caccgccacc gccaccggct gagtctgcag
     1561 tcctccgaaa cgggccctct agactcgagc ggccgctcga gcctaagctt cgaattcacc
     1621 accatggcct ccggtgtggc tgtctctgat ggtgtcatca aggtgttcaa cgacatgaag
     1681 gtgcgtaagt cttcaacgcc agaggaggtg aagaagcgca agaaggcggt gctcttctgc
     1741 ctgagtgagg acaagaagaa catcatcctg gaggagggca aggagatcct ggtgggcgat
     1801 gtgggccaga ctgtcgacga tccctacgcc acctttgtca agatgctgcc agataaggac
     1861 tgccgctatg ccctctatga tgcaacctat gagaccaagg agagcaagaa ggaggatctg
     1921 gtgtttatct tctgggcccc cgagtctgcg ccccttaaga gcaaaatgat ttatgccagc
     1981 tccaaggacg ccatcaagaa gaagctgaca gggatcaagc atgaattgca agcaaactgc
     2041 tacgaggagg tcaaggaccg ctgcaccctg gcagagaagc tggggggcag tgcggtcatc
     2101 tccctggagg gcaagccttt gttatcccgg gatccgatgg cctcctccga ggacgtcatc
     2161 aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag
     2221 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     2281 accaagggcg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt ccagtacggc
     2341 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     2401 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     2461 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     2521 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
     2581 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agatgaggct gaagctgaag
     2641 gacggcggcc actacgacgc cgaggtcaag accacctaca tggccaagaa gcccgtgcag
     2701 ctgcccggcg cctacaagac cgacatcaag ctggacatca cctcccacaa cgaggactac
     2761 accatcgtgg aacagtacga gcgcgccgag ggccgccact ccaccggcgc ctaagaattg
     2821 gccgcgactc tagtggatcc gagctcggta ccaagcttaa gtttctagat cataatcagc
     2881 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     2941 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     3001 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3061 agttgtggtt tgtccaaact catcaatgta tcttaatcga ctcgaagatc tgggcgtggt
     3121 taagggtggg aaagaatata taaggtgggg gtcttatgta gttttgtatc tgttttgcag
     3181 cagccgccgc cgccatgagc accaactcgt ttgatggaag cattgtgagc tcatatttga
     3241 caacgcgcat gcccccatgg gccggggtgc gtcagaatgt gatgggctcc agcattgatg
     3301 gtcgccccgt cctgcccgca aactctacta ccttgaccta cgagaccgtg tctggaacgc
     3361 cgttggagac tgcagcctcc gccgccgctt cagccgctgc agccaccgcc cgcgggattg
     3421 tgactgactt tgctttcctg agcccgcttg caagcagtgc agcttcccgt tcatccgccc
     3481 gcgatgacaa gttgacggct cttttggcac aattggattc tttgacccgg gaacttaatg
     3541 tcgtttctca gcagctgttg gatctgcgcc agcaggtttc tgccctgaag gcttcctccc
     3601 ctcccaatgc ggtttaaaac ataaataaaa aaccagactc tgtttggatt tggatcaagc
     3661 aagtgtcttg ctgtctttat ttaggggttt tgcgcgcgcg gtaggcccgg gaccagcggt
     3721 ctcggtcgtt gagggtcctg tgtatttttt ccaggacgtg gtaaaggtga ctctggatgt
     3781 tcagatacat gggcataagc ccgtctctgg ggtggaggta gcaccactgc agagcttcat
     3841 gctgcggggt ggtgttgtag atgatccagt cgtagcagga gcgctgggcg tggtgcctaa
     3901 aaatgtcttt cagtagcaag ctgattgcca ggggcaggcc cttggtgtaa gtgtttacaa
     3961 agcggttaag ctgggatggg tgcatacgtg gggatatgag atgcatcttg gactgtattt
     4021 ttaggttggc tatgttccca gccatatccc tccggggatt catgttgtgc agaaccacca
     4081 gcacagtgta tccggtgcac ttgggaaatt tgtcatgtag cttagaagga aatgcgtgga
     4141 agaacttgga gacgcccttg tgacctccaa gattttccat gcattcgtcc ataatgatgg
     4201 caatgggccc acgggcggcg gcctgggcga agatatttct gggatcacta acgtcatagt
     4261 tgtgttccag gatgagatcg tcataggcca tttttacaaa gcgcgggcgg agggtgccag
     4321 actgcggtat aatggttcca tccggcccag gggcgtagtt accctcacag atttgcattt
     4381 cccacgcttt gagttcagat ggggggatca tgtctacctg cggggcgatg aagaaaacgg
     4441 tttccggggt aggggagatc agctgggaag aaagcaggtt cctgagcagc tgcgacttac
     4501 cgcagccggt gggcccgtaa atcacaccta ttaccggctg caactggtag ttaagagagc
     4561 tgcagctgcc gtcatccctg agcagggggg ccacttcgtt aagcatgtcc ctgactcgca
     4621 tgttttccct gaccaaatcc gccagaaggc gctcgccgcc cagcgatagc agttcttgca
     4681 aggaagcaaa gtttttcaac ggtttgagac cgtccgccgt aggcatgctt ttgagcgttt
     4741 gaccaagcag ttccaggcgg tcccacagct cggtcacctg ctctacggca tctcgatcca
     4801 gcatatctcc tcgtttcgcg ggttggggcg gctttcgctg tacggcagta gtcggtgctc
     4861 gtccagacgg gccagggtca tgtctttcca cgggcgcagg gtcctcgtca gcgtagtctg
     4921 ggtcacggtg aaggggtgcg ctccgggctg cgcgctggcc agggtgcgct tgaggctggt
     4981 cctgctggtg ctgaagcgct gccggtcttc gccctgcgcg tcggccaggt agcatttgac
     5041 catggtgtca tagtccagcc cctccgcggc gtggcccttg gcgcgcagct tgcccttgga
     5101 ggaggcgccg cacgaggggc agtgcagact tttgagggcg tagagcttgg gcgcgagaaa
     5161 taccgattcc ggggagtagg catccgcgcc gcaggccccg cagacggtct cgcattccac
     5221 gagccaggtg agctctggcc gttcggggtc aaaaaccagg tttcccccat gctttttgat
     5281 gcgtttctta cctctggttt ccatgagccg gtgtccacgc tcggtgacga aaaggctgtc
     5341 cgtgtccccg tatacagact tgagagggag tttaaacgaa ttcaatagct tgttgcatgg
     5401 gcggcgatat aaaatgcaag gtgctgctca aaaaatcagg caaagcctcg cgcaaaaaag
     5461 aaagcacatc gtagtcatgc tcatgcagat aaaggcaggt aagctccgga accaccacag
     5521 aaaaagacac catttttctc tcaaacatgt ctgcgggttt ctgcataaac acaaaataaa
     5581 ataacaaaaa aacatttaaa cattagaagc ctgtcttaca acaggaaaaa caacccttat
     5641 aagcataaga cggactacgg ccatgccggc gtgaccgtaa aaaaactggt caccgtgatt
     5701 aaaaagcacc accgacagct cctcggtcat gtccggagtc ataatgtaag actcggtaaa
     5761 cacatcaggt tgattcacat cggtcagtgc taaaaagcga ccgaaatagc ccgggggaat
     5821 acatacccgc aggcgtagag acaacattac agcccccata ggaggtataa caaaattaat
     5881 aggagagaaa aacacataaa cacctgaaaa accctcctgc ctaggcaaaa tagcaccctc
     5941 ccgctccaga acaacataca gcgcttccac agcggcagcc ataacagtca gccttaccag
     6001 taaaaaagaa aacctattaa aaaaacacca ctcgacacgg caccagctca atcagtcaca
     6061 gtgtaaaaaa gggccaagtg cagagcgagt atatatagga ctaaaaaatg acgtaacggt
     6121 taaagtccac aaaaaacacc cagaaaaccg cacgcgaacc tacgcccaga aacgaaagcc
     6181 aaaaaaccca caacttcctc aaatcgtcac ttccgttttc ccacgttacg tcacttccca
     6241 ttttaagaaa actacaattc ccaacacata caagttactc cgccctaaaa cctacgtcac
     6301 ccgccccgtt cccacgcccc gcgccacgtc acaaactcca ccccctcatt atcatattgg
     6361 cttcaatcca aaataaggta tattattgat gatgttaatt aacatgcatg gatccatatg
     6421 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     6481 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6541 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6601 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6661 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6721 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6781 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6841 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6901 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6961 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7021 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7081 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7141 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7201 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7261 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7321 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7381 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7441 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7501 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7561 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7621 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7681 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc agccatgaga
     7741 ttatcaaaaa ggatcttcac ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg
     7801 tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca
     7861 aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta
     7921 tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc
     7981 tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaagc
     8041 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     8101 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     8161 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     8221 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     8281 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     8341 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     8401 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     8461 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     8521 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     8581 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     8641 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     8701 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     8761 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     8821 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tttgttaaaa
     8881 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     8941 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     9001 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     9061 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     9121 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     9181 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     9241 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccg
//
