LOCUS       sequence-490277-        6009 bp    DNA     circular SYN 22-OCT-2025
DEFINITION  Note- This plasmid contains Keratin 18. Localization: Cytokeratin, 
            Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 22, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Keratin-17
FEATURES             Location/Qualifiers
     source          1..6009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        81..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..588
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     539..559
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1955..1964
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1961..2695
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     polyA_signal    2817..2938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2854..2873)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2908..2927
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2945..3400)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3082..3103)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3294..3313
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3427..3531
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3533..3890
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3533..3724
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3556..3576)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3741..3876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3925..4719
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3925..4719
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3979..3998)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4589..4608
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4907..4926)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4951..4998
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5327..5915
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5816..5835
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      421 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      481 ttttggcacc aaaatcaacg ggacttttca aaatgtcgta acaactccgc cccattgacg
      541 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
      601 cgtcagatcc gctagcacca tgagcttcac cactcgctcc accttctcca ccaactaccg
      661 gtccctgggc tctgtccagg cgcccagcta cggcgcccgg ccggtcagca gcgcggccag
      721 cgtctatgca ggcgctgggg gctctggttc ccggatctcc gtgtcccgct ccaccagctt
      781 caggggcggc atggggtccg ggggcctggc caccgggata gccgggggtc tggcaggaat
      841 gggaggcatc cagaacgaga aggagaccat gcaaagcctg aacgaccgcc tggcctctta
      901 cctggacaga gtgaggagcc tggagaccga gaaccggagg ctggagagca aaatccggga
      961 gcacttggag aagaagggac cccaggtcag agactggagc cattacttca agatcatcga
     1021 ggacctgagg gctcagatct tcgcaaatac tgtggacaat gcccgcatcg ttctgcagat
     1081 tgacaatgcc cgtcttgctg ctgatgactt tagagtcaag tatgagacag agctggccat
     1141 gcgccagtct gtggagaacg acatccatgg gctccgcaag gtcattgatg acaccaatat
     1201 cacacgactg cagctggaga cagagatcga ggctctcaag gaggagctgc tcttcatgaa
     1261 gaagaaccac gaagaggaag taaaaggcct acaagcccag attgccagct ctgggttgac
     1321 cgtggaggta gatgccccca aatctcagga cctcgccaag atcatggcag acatccgggc
     1381 ccaatatgac gagctggctc ggaagaaccg agaggagcta gacaagtact ggtctcagca
     1441 gattgaggag agcaccacag tgctcaccac acagtctgct gaggttggag ctgctgagac
     1501 gacgctcaca gagctgagac gtacagtcca gtccttggag atcgacctgg actccatgag
     1561 aaatctgaag gccagcttgg agaacagcct gagggaggtg gaggcccgct acgccctaca
     1621 gatggagcag ctcaacggga tcctgctgca ccttgagtca gagctggcac agacccgggc
     1681 agagggacag cgccaggccc aggagtatga ggccctgctg aacatcaagg tcaagctgga
     1741 ggctgagatc gccacctacc gccgcctgct ggaagatggc gaggacttta atcttggtga
     1801 tgccttggac agcagcaact ccatgcaaac catccaaaag accaccaccc gccggatagt
     1861 ggatggcaaa gtggtgtctg agaccaatga caccaaagtt ctgaggcatc gaattctgca
     1921 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga
     1981 gctgatcaag gagaacatgc acatgaagct gtacatggaa ggcaccgtga acaaccacca
     2041 cttcaagtgc accaccgaag gggagggcaa gccctacgag ggcacccaga cccagaggat
     2101 taaggtggtg gagggaggcc ccctgccgtt cgcattcgac atcctggcca cctgctttat
     2161 gtacgggagc aagaccttca tcaaccacac ccagggcatc cccgatttct ttaagcagtc
     2221 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgcttac
     2281 cgttacccag gacaccagcc tccaggacgg ctgcttgatc tacaacgtca agctcagagg
     2341 ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccac
     2401 caccgagacc ctgtaccccg ctgacggcgg cctggaaggc agatgcgaca tggccctgaa
     2461 gctcgtgggc gggggccacc tgcactgcaa cctgaagacc acatacagat ccaagaaacc
     2521 cgctaagaac ctcaagatgc ccggcgtcta ctttgtggac cgcagactgg aaagaatcaa
     2581 ggaggccgac aatgagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga
     2641 cctccctagc aaactggggc acaaacttaa tggcatggac gagctgtaca agtaagcggc
     2701 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     2761 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     2821 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     2881 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag
     2941 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     3001 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     3061 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     3121 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     3181 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     3241 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     3301 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     3361 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     3421 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3481 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     3541 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     3601 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     3661 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     3721 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3781 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3841 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     3901 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     3961 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     4021 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     4081 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     4141 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     4201 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     4261 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     4321 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     4381 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     4441 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     4501 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     4561 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     4621 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     4681 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     4741 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     4801 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     4861 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     4921 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     4981 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     5041 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     5101 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     5161 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5221 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5281 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5341 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5401 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5461 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5521 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5581 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5641 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5701 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5761 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5821 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5881 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5941 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6001 ctgtggata
//
