LOCUS       sequence-490280-       10967 bp    DNA     circular SYN 22-OCT-2025
DEFINITION  Lentiviral expression of shRNA targeting Ecad and Ecad-tdtomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Wu SK, Gomez GA, Michael M, Verma S, Cox HL, Lefevre JG, Parton RG, 
            Hamilton NA, Neufeld Z, Yap AS
  TITLE     Cortical F-actin stabilization generates apical-lateral patterns of 
            junctional contractility that integrate cells into epithelia.
  JOURNAL   Nat Cell Biol. 2014 Feb;16(2):167-78. doi: 10.1038/ncb2900. Epub 
            2014 Jan 12.
  PUBMED    24413434
REFERENCE   2  (bases 1 to 10967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 22, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2900"; 
            journalName: "Nat Cell Biol"; date: "2014-02"; volume: "16"; issue: 
            "2"; pages: "167-78"
COMMENT     Sequence Label: pLL5.0 E-cadherin shRNA/Ecad-tdTomato
FEATURES             Location/Qualifiers
     source          1..10967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..54)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        226..605
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        607..805
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     760..780
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             823..1003
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1050..1175
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1672..1905
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2090..2134
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2432..2549
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2604..2916
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2835..2855
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     2973..2989
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3031..3064)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6295..6304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6301..7731
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    complement(7757..7790)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7846..8432
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7899..7919)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8317..8328)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8435..8451)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8961..9141
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(9203..9791)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9283..9302)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9962..10753,10754..10822))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10585..10604
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10823..10927)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
       61 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
      121 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
      181 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
      241 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      301 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      361 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      421 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      481 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      541 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      601 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      661 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      721 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      781 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac
      841 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      901 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      961 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     1021 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     1081 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     1141 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     1201 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     1261 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     1321 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1381 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1441 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1501 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     1561 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     1621 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     1681 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     1741 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     1801 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     1861 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     1921 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     1981 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     2041 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     2101 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     2161 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     2221 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     2281 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     2341 agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat
     2401 tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     2461 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2521 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     2581 tttggttagt accgcgctct agagatccga cgcgccatct ctaggcccgc gccggccccc
     2641 tcgcacagac ttgtgggaga agctcggcta ctcccctgcc ccggttaatt tgcatataat
     2701 atttcctagt aactatagag gcttaatgtg cgataaaaga cagataatct gttcttttta
     2761 atactagcta cattttacat gataggcttg gatttctata agagatacaa atactaaatt
     2821 attattttaa aaaacagcac aaaaggaaac tcaccctaac tgtaaagtaa ttgtgtgttt
     2881 tgagactata aatatccctt ggagaaaagc cttgtttgtc agctactcta gttctgttca
     2941 agagacagaa ctagagtagc tgactttttt ctcgaggtcg acggtatcga taagctcgct
     3001 tcacgagatt ccagcaggtc gagggaccta ataacttcgt atagcataca ttatacgaag
     3061 ttatattaag ggttccaagc ttaagcggcc gctgaaagac cccacctgta ggtttggcaa
     3121 gctagcttaa gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag
     3181 ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg
     3241 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc
     3301 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     3361 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
     3421 ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag
     3481 actgcgtcgc ccgggtaccc gtattcccaa taaagcctct tgctgtttgc atccgaatcg
     3541 tggactcgct gatccttggg agggtctcct cagattgatt gactgcccac ctcgggggtc
     3601 tttcatcgaa ttctgcagtc gacggtaccg cggatgggag cccggtgccg cagcttttcc
     3661 gcgctcctgc tcctgctgca ggtctcctca tggctttgcc aggagctgga gcctgagtcc
     3721 tgcagtcccg gcttcagttc cgaggtctac accttcccgg tgccggagag gcacctggag
     3781 agaggccatg tcctgggcag agtgagattt gaaggatgca ccggccggcc aaggacagcc
     3841 ttcttttcgg aagactcccg attcaaagtg gcgacagacg gcaccatcac agtgaagcgg
     3901 catctaaagc tccacaagct ggagaccagt ttcctcgtcc gcgcccggga ctccagtcat
     3961 agggagctgt ctaccaaagt gacgctgaag tccatggggc accaccatca ccggcaccac
     4021 caccgcgacc ctgcctctga atccaaccca gagctgctca tgtttcccag cgtgtaccca
     4081 ggtctcagaa gacagaaacg agactgggtc atccctccca tcagctgccc cgaaaatgaa
     4141 aagggtgaat tcccaaagaa cctggttcag atcaaatcca acagggacaa agaaacaaag
     4201 gttttctaca gcatcaccgg ccaaggagct gacaaacccc ccgttggcgt tttcatcatt
     4261 gagagggaga caggctggct gaaagtgaca cagcctctgg atagagaagc cattgccaag
     4321 tacatcctct attctcatgc cgtgtcatca aatggggaag cggtggagga tcccatggag
     4381 atagtgatca cagtgacaga tcagaatgac aacaggccag agtttaccca ggaggtgttt
     4441 gagggatccg ttgcagaagg cgctgttcca ggaacctccg tgatgaaggt ctcagccacc
     4501 gatgcagacg atgacgtcaa cacctacaac gctgccatcg cctacaccat cgtcagccag
     4561 gatcctgagc tgcctcacaa aaacatgttc actgtcaata gggacaccgg ggtcatcagt
     4621 gtgctcacct ctgggctgga ccgagagagt taccctacat acactctggt ggttcaggct
     4681 gctgaccttc aaggcgaagg cttgagcaca acagccaagg ctgtgatcac tgtcaaggat
     4741 attaatgaca acgctcctgt cttcaacccg agcacgtatc agggtcaagt gcctgagaat
     4801 gaggtcaatg cccggatcgc cacactcaaa gtgaccgatg atgatgcccc caacactccg
     4861 gcgtggaaag ctgtgtacac cgtagtcaac gatcctgacc agcagttcgt tgtcgtcaca
     4921 gaccccacga ccaatgatgg cattttgaaa acagccaagg gcttggattt tgaggccaag
     4981 cagcaataca tccttcatgt gagagtggag aacgaggaac cctttgaggg gtctcttgtc
     5041 ccttccacag ccactgtcac tgtggacgtg gtagacgtga atgaagcccc catctttatg
     5101 cctgcggaga ggagagtcga agtgcccgaa gactttggtg tgggtcagga aatcacatct
     5161 tataccgctc gagagccgga cacgttcatg gatcagaaga tcacgtatcg gatttggagg
     5221 gacactgcca actggctgga gattaaccca gagactggtg ccattttcac gcgcgctgag
     5281 atggacagag aagacgctga gcatgtgaag aacagcacat atgtagctct catcatcgcc
     5341 acagatgatg gttcacccat tgccactggc acgggcactc ttctcctggt cctgttagac
     5401 gtcaatgaca acgctcccat cccagaacct cgaaacatgc agttctgcca gaggaaccca
     5461 cagcctcata tcatcaccat cttggatcca gaccttcccc ccaacacgtc cccctttact
     5521 gctgagctaa cccatggggc cagcgtcaac tggaccattg agtataatga cgcagctcaa
     5581 gaatctctca ttttgcaacc aagaaaggac ttagagattg gcgaatacaa aatccatctc
     5641 aagctcgcgg ataaccagaa caaagaccag gtgaccacgt tggacgtcca tgtgtgtgac
     5701 tgtgaaggga cggtcaacaa ctgcatgaag gcgggaatcg tggcagcagg attgcaagtt
     5761 cctgccatcc tcggaatcct tggagggatc ctcgccctgc tgattctgat cctgctgctc
     5821 ctactgtttc tacggaggag aacggtggtc aaagagcccc tgctgccacc agatgatgat
     5881 acccgggaca atgtgtatta ctatgatgaa gaaggaggtg gagaagaaga ccaggacttt
     5941 gatttgagcc agctgcacag gggcctggat gcccgaccgg aagtgactcg aaatgatgtg
     6001 gctcccaccc tcatgagcgt gccccagtat cgtccccgtc ctgccaatcc tgatgaaatt
     6061 ggaaacttca tcgatgaaaa cctgaaggca gccgacagcg accccacggc acccccttac
     6121 gactctctgt tggtgttcga ttacgagggc agtggttctg aagccgctag cctgagctca
     6181 ctgaactcct ctgagtcgga tcaggaccag gactacgatt atctgaacga gtggggcaac
     6241 cgattcaaga agctggcgga catgtacggc ggtggcgagg acgacaaacc ggtcgccacc
     6301 atggtgagca agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
     6361 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     6421 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     6481 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
     6541 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     6601 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
     6661 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
     6721 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     6781 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
     6841 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
     6901 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
     6961 cgccaccacc tgttcctggg gcatggcacc ggcagcaccg gcagcggcag ctccggcacc
     7021 gcctcctccg aggacaacaa catggccgtc atcaaagagt tcatgcgctt caaggtgcgc
     7081 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     7141 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     7201 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
     7261 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     7321 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
     7381 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
     7441 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
     7501 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
     7561 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
     7621 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
     7681 gagggccgcc accacctgtt cctgtacggc atggacgagc tgtacaagta atgcaggcaa
     7741 ttcgtcgagg gacctaataa cttcgtatag catacattat acgaagttat acatgtttaa
     7801 gggttccggt tccactaggt acaattcgat atcaagctta tcgataatca acctctggat
     7861 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7921 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7981 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     8041 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     8101 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     8161 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     8221 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     8281 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8341 ccttcccggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     8401 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc
     8461 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     8521 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     8581 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8641 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     8701 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     8761 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     8821 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     8881 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     8941 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     9001 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9061 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9121 cagtgtggaa aatctctagc agcatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9181 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9241 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9301 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9361 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9421 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9481 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9541 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9601 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     9661 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9721 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9781 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9841 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9901 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9961 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    10021 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    10081 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    10141 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    10201 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    10261 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10321 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10381 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10441 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10501 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10561 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10621 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10681 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10741 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10801 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10861 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10921 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcg
//
