LOCUS       sequence-490370-        9344 bp    DNA     circular SYN 23-OCT-2025
DEFINITION  Low Density Lipoprotein Receptor N-terminally tagged with Green 
            Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved 
            leaving the GFP attached to the mature LDLR protien..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Jenkins B, Decker H, Bentley M, Luisi J, Banker G
  TITLE     A novel split kinesin assay identifies motor proteins that interact 
            with distinct vesicle populations.
  JOURNAL   J Cell Biol. 2012 Aug 20;198(4):749-61. doi: 10.1083/jcb.201205070.
  PUBMED    22908316
REFERENCE   2  (bases 1 to 9344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2012-08-20- 20"; volume: "198"; 
            issue: "4"; pages: "749-61"
COMMENT     Sequence Label: pBa-LSS-GFP-LDLR wt
FEATURES             Location/Qualifiers
     source          1..9344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        418..691
                     /label=chicken beta-actin promoter
     primer_bind     1634..1656
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(1805..1807,1808..2518)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1805..1807,1808..2518)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1847..1868)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2108..2127)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2455..2476
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(4881..4892)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5180..5314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5217..5236)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5271..5290
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5345..5361)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5345..5361)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5358..5380)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5369..5385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5393..5399,5400..5417,5418..5423))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5438..5459
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5576..5593)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5747..6335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5827..5846)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6506..7297,7298..7366))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7129..7148
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7367..7471)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7539..7557
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7595..7617)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7717..7736
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7930..7952
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7944..7961
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7945..7961
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctagtgcatg catacccctc ggtgttattg ctgctctgtg cacgcacgct cattgtatca
       61 cttcatccca gtgcatgcac tcacactgga gcgattgctg ctcggtgcac gcacactcat
      121 tgtatcactg cagctcagtg gctgcacgca caccggtgtt attgctgctc ggtgcgtgca
      181 tgcacatcag tgtcgctgca gctcagtgca tgcacgctca ttgcccatcg ctatccctgc
      241 ctctcctgct ggcgctcccc gggaggtgac ttcaagggga ccgcaggacc acctcggggg
      301 tggggggagg gctgcacacg cggaccccgc tccccctccc caacaaagca ctgtggaatc
      361 aaaaaggggg gaggggggat ggaggggcgc gtcacacccc cgccccacac cctcacctcg
      421 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
      481 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggcgcgc
      541 gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc
      601 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
      661 gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt
      721 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
      781 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg
      841 acggctcgtt tcttttctgt ggctgcgtga aagccttaaa gggctccggg agggcccttt
      901 gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt
      961 gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     1021 tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct
     1081 gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg
     1141 gcgcggcggt cgggctgtaa cccccccctg cacccccctc cccgagttgc tgagcacggc
     1201 ccggcttcgg gtgcggggct ccgtgcgggg cgtggcgcgg ggctcgccgt gccgggcggg
     1261 gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg
     1321 ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc
     1381 cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt
     1441 gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg
     1501 tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt
     1561 ccccttctcc atctccagcc tcggggctgt ccgcaggggg acggctgcct tcggggggga
     1621 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gggtttatat cttcccttct
     1681 ctgttcctcc gcagccccca agcttggcgc gcctagcacc atggggccct ggggctggaa
     1741 attgcgctgg accgttgcct tgcttcttgc cgctgctgga actgcagttg gcgacagaag
     1801 tactgtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1861 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1921 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1981 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     2041 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     2101 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2161 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2221 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     2281 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     2341 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2401 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     2461 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagac
     2521 aggaggggga gaattcgaca gatgtgaaag aaacgagttc cagtgccaag acgggaaatg
     2581 catctcctac aagtgggtct gcgatggcag cgctgagtgc caggatggct ctgatgagtc
     2641 ccaggagacg tgcttgtctg tcacctgcaa atccggggac ttcagctgtg ggggccgtgt
     2701 caaccgctgc attcctcagt tctggaggtg cgatggccaa gtggactgcg acaacggctc
     2761 agacgagcaa ggctgtcccc ccaagacgtg ctcccaggac gagtttcgct gccacgatgg
     2821 gaagtgcatc tctcggcagt tcgtctgtga ctcagaccgg gactgcttgg acggctcaga
     2881 cgaggcctcc tgcccggtgc tcacctgtgg tcccgccagc ttccagtgca acagctccac
     2941 ctgcatcccc cagctgtggg cctgcgacaa cgaccccgac tgcgaagatg gctcggatga
     3001 gtggccgcag cgctgtaggg gtctttacgt gttccaaggg gacagtagcc cctgctcggc
     3061 cttcgagttc cactgcctaa gtggcgagtg catccactcc agctggcgct gtgatggtgg
     3121 ccccgactgc aaggacaaat ctgacgagga aaactgcgct gtggccacct gtcgccctga
     3181 cgaattccag tgctctgatg gaaactgcat ccatggcagc cggcagtgtg accgggaata
     3241 tgactgcaag gacatgagcg atgaagttgg ctgcgttaat gtgacactct gcgagggacc
     3301 caacaagttc aagtgtcaca gcggcgaatg catcaccctg gacaaagtct gcaacatggc
     3361 tagagactgc cgggactggt cagatgaacc catcaaagag tgcgggacca acgaatgctt
     3421 ggacaacaac ggcggctgtt cccacgtctg caatgacctt aagatcggct acgagtgcct
     3481 gtgccccgac ggcttccagc tggtggccca gcgaagatgc gaagatatcg atgagtgtca
     3541 ggatcccgac acctgcagcc agctctgcgt gaacctggag ggtggctaca agtgccagtg
     3601 tgaggaaggc ttccagctgg acccccacac gaaggcctgc aaggctgtgg gctccatcgc
     3661 ctacctcttc ttcaccaacc ggcacgaggt caggaagatg acgctggacc ggagcgagta
     3721 caccagcctc atccccaacc tgaggaacgt ggtcgctctg gacacggagg tggccagcaa
     3781 tagaatctac tggtctgacc tgtcccagag aatgatctgc agcacccagc ttgacagagc
     3841 ccacggcgtc tcttcctatg acaccgtcat cagcagggac atccaggccc ccgacgggct
     3901 ggctgtggac tggatccaca gcaacatcta ctggaccgac tctgtcctgg gcactgtctc
     3961 tgttgcggat accaagggcg tgaagaggaa aacgttattc agggagaacg gctccaagcc
     4021 aagggccatc gtggtggatc ctgttcatgg cttcatgtac tggactgact ggggaactcc
     4081 cgccaagatc aagaaagggg gcctgaatgg tgtggacatc tactcgctgg tgactgaaaa
     4141 cattcagtgg cccaatggca tcaccctaga tctcctcagt ggccgcctct actgggttga
     4201 ctccaaactt cactccatct caagcatcga tgtcaatggg ggcaaccgga agaccatctt
     4261 ggaggatgaa aagaggctgg cccacccctt ctccttggcc gtctttgagg acaaagtatt
     4321 ttggacagat atcatcaacg aagccatttt cagtgccaac cgcctcacag gttccgatgt
     4381 caacttgttg gctgaaaacc tactgtcccc agaggatatg gtcctcttcc acaacctcac
     4441 ccagccaaga ggagtgaact ggtgtgagag gaccaccctg agcaatggcg gctgccagta
     4501 tctgtgcctc cctgccccgc agatcaaccc ccactcgccc aagtttacct gcgcctgccc
     4561 ggacggcatg ctgctggcca gggacatgag gagctgcctc acagaggctg aggctgcagt
     4621 ggccacccag gagacatcca ccgtcaggct aaaggtcagc tccacagccg taaggacaca
     4681 gcacacaacc acccggcctg ttcccgacac ctcccggctg cctggggcca cccctgggct
     4741 caccacggtg gagatagtga caatgtctca ccaagctctg ggcgacgttg ctggcagagg
     4801 aaatgagaag aagcccagta gcgtgagggc tctaagcttg gtcctcccca tcgtgctcct
     4861 cgtcttcctt tgcctggggg tcttccttct atggaagaac tggcggctta agaacatcaa
     4921 cagcatcaac tttgacaacc ccgtctatca gaagaccaca gaggatgagg tccacatttg
     4981 ccacaaccag gacggctaca gctacccctc gagacagatg gtcagtctgg aggatgacgt
     5041 ggcgtgacgg accgtctaat ctagataagt aatgatcata atcagccata tcacatctgt
     5101 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     5161 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     5221 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5281 caaactcatc aatgtatctt atcatgtctg gatccccggg taccgagctc gaattcgtaa
     5341 tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5401 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     5461 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     5521 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     5581 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5641 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5701 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5761 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5821 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5881 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5941 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6001 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6061 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6121 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6181 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6241 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6301 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6361 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6421 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6481 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6541 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6601 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6661 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6721 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6781 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6841 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6901 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6961 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7021 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7081 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7141 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7201 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7261 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7321 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7381 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7441 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     7501 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     7561 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     7621 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     7681 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     7741 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     7801 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     7861 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     7921 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagttg ggatctttgc
     7981 attggcccac ggctctcagg atggggatgc tccccttcag cacccggttc cccttggaaa
     8041 ctgatggtcc tggctctgtg gcatggcagt ggcactgtga ggagccccct accagcagca
     8101 cacagtgggg ttggcactgc cacgctccgg atgccgcgct ctgatccaac cccataatca
     8161 agggaacccg aattgcccca tcattgcccc caccaccccc atcctgccgg gccctcacac
     8221 cccacgctgc cttctggtga cattccccag cccaaaccca cggcttcatg gctaccgcgg
     8281 ggcatttccc attgccgccc cattatcagc tctgcacacc tcccgctgta cccatgcctc
     8341 gtggctgccc ttctttgacg tataatcttc taattaatac ccggccttgt caaagtggag
     8401 cacaaacgtt aattaattcc ccagcaggca ggtaattaac agtgtgactc cctttttgct
     8461 gcgagtgggg ctgatacaga gagatgtggc actatggagc ccacggggtc ctggcactgg
     8521 gtgcccacgg aggtccccat gtgctgcagt gtcaccgcct ccgaggagac agtattgtcc
     8581 ctgcggtgtc cctgcagctc agctctgtcc acagggccac ctccagtttg gaggggacac
     8641 aatgcagccc cgatgcaacc catcctcgca gcatcccagg gacaaagacc ccactgcaag
     8701 accgcacaca gggctgggtc ccgctcccct aatatctaca gtgcttcttg catggcccct
     8761 taatcaatgc agttaatcag catgcgctca tgcaccgctc tggagctgca aagcccctcg
     8821 cagcgctgct caccaacacc gcgcaccgcc ccggcccagc ctgcagcacg cgctgcaaac
     8881 aggaaagaaa caaaatattg cccaaatgta ggcaaaggca ttcggctgcc ttgacctccg
     8941 ccgggccggg ccctgcctga ctcagctcct tactcagcgc tcgcttcctc cctccggctg
     9001 ccaccgccgc agcgcacacc ctgacaaaga gtggccctta acgggctctg aggtgcaccc
     9061 agcagtgcac tcagcagtcc aagggccggc ctggaggttt gcaccgctac gtgctgacat
     9121 tagcattgaa cttggccctg ggtagtgctg caggccgggc ggggtgggtg tagagagtgc
     9181 agcgcgcgtt gcacccggtg ccccttcccc tcccttgcat cccagcaggc tgcaccccag
     9241 caccaggccc gtgcatgcat gctcctggtg ttattgcagc ctggtgcatg catgcgtctt
     9301 gatggtgcag cgctgtgcat gcatcctcct tggtgtgtag cagt
//
