LOCUS       sequence-490468-       11624 bp    DNA     circular SYN 23-OCT-2025
DEFINITION  Targets high levels of recombinant protein to apoplast. Empty 
            vector. Clone via SapI restriction site. mCherry dropout cassette 
            will enable Red/White screening in E.coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11624)
  AUTHORS   Chartrand-Pleau C, Gallipeau-Burns E, Jain A, Boddy V, Fakhouri P, 
            Rehmani S, Thomas T, Drapeau M, VanderBurgt JT, Boudigou A, Damry 
            AM, MacLean AM
  TITLE     pTARGEX Vectors: A Versatile Toolbox for Plant-Based Protein 
            Expression
  JOURNAL   Front. Synth. Biol. 3:1619871
REFERENCE   2  (bases 1 to 11624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front. 
            Synth. Biol. 3:1619871"
COMMENT     Sequence Label: pTARGEX-APO
FEATURES             Location/Qualifiers
     source          1..11624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        110..155
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    434..455
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(470..475,476..493,494..500)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    508..524
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             564..586
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      1374..1401
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1636..1888
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2036..2381
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3854..4030
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4596..4775
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4628..4811
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(4832..4834,4835..4837,4838..5548)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      5937..6189
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6280..6304
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      6672..7303
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(7393..8541)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8840..9634)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     complement(10224..10241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10475..10494)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10926..10950
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     11089..11108
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11233..11255
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11247..11264
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11248..11264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
       61 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
      121 cctctatata aggaagttca tttcatttgg agaggtttaa gagacgcaac cacaacgctc
      181 taacgcaatc aatctacatt atattaaacg tctctaaaaa gaaaccatgg gattttttct
      241 cttttcacaa atgccctcat tttttcttgt gtcgacactt ctcttattcc taataatatc
      301 tcactcttct catgccggct gaagagcatc aatcaatacg caaaccgcct ctccccgcgc
      361 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      421 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      481 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaagaccac
      541 aacggtttcc ctctagaaat aattttgttt aactttaaga aggagatata catatggttt
      601 ccaagggaga ggaagacaat atggcgatta tcaaagagtt catgcgcttc aaggtccaca
      661 tggaaggaag tgtgaacggg catgagttcg aaatagaagg cgaaggtgag ggtcgcccgt
      721 acgaaggtac gcaaaccgca aagctgaagg ttactaaggg tgggcctctg ccgttcgcat
      781 gggacatctt atctccgcaa ttcatgtacg gtagtaaggc ttacgtaaag caccccgccg
      841 atattcccga ctacttgaag ttaagtttcc cagaggggtt caagtgggag agagtaatga
      901 atttcgagga cggcggggta gtcaccgtaa cccaagactc atctcttcaa gacggtgagt
      961 ttatatacaa ggtgaagctg cgcgggacga acttcccatc tgacggacca gtgatgcaga
     1021 agaagactat gggatgggaa gccagtagcg aaagaatgta cccagaagac ggggccctta
     1081 aaggagaaat taagcagcgg ctcaagttga aggatggcgg gcactatgat gcagaggtca
     1141 aaaccaccta caaggcaaag aaaccagtac aattgcccgg tgcgtacaac gtaaacatta
     1201 aattagacat cacttctcat aatgaggact acacaatagt ggagcaatac gagcgagcag
     1261 aggggagaca ctctacgggt ggtatggacg agttgtataa ataagaattc gaagcttgat
     1321 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccatcac actggctcac
     1381 cttcgggtgg gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt
     1441 aatccggctt ttttattatt tgagttgctc ttcatgatga gtataaataa actgttattc
     1501 ggtgtgcatt tctatgtttg gtgagcggtt ttctgtgctc agagtgtgtt tattttatgt
     1561 aatttaattt ctttgtgagc tcctgtttag caggtcgtcc cttcagcaag gacacaaaaa
     1621 gattttaatt ttattgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     1681 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     1741 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     1801 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     1861 cgcgcggtgt catctatgtt actagatcgg cgcgccagct tggcgtaatc atggtcatag
     1921 ctgttgcgat taataattcc acgcggatcg cattgagaac ccggaatatc gcgaaacagg
     1981 taccccccta ctccaaaaat gtcaaagata cagtctcaga agaccaaagg gctattgaga
     2041 cttttcaaca aagggtaatt tcgggaaacc tcctcggatt ccattgccca gctatctgtc
     2101 acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat cattgcgata
     2161 aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     2221 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     2281 gatgtgacat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
     2341 cttcctctat ataaggaagt tcatttcatt tggagaggac agcccaagct tcgactctag
     2401 aggatcccct taaatcgata tgagcaccgc caagatgagc gctggtgagt tctctaagac
     2461 ctacggcacc aaggatagcc ggtctgtgaa tgattgctac gccatctaca gcggcaacgg
     2521 catcaacttc atcaacctgt tcatgcacac caacaccggc atcaagagcg ccttctctat
     2581 taacgacctt ggccggaacg aggacattaa gcttcatgag gccgagatca tcaacagctt
     2641 gcacccgtac tcattcttcg acaagttcgg cctggatatc atcctgtgca accacgtgat
     2701 ggacatcatc gtgactaagc ctggccttaa gaacaccgga tgcaagttcc agatgcacaa
     2761 ccagatcttc aacccgaatg aggatattct ggctaagacc cctggcatca tcagcgaaga
     2821 gaacttctac gacctgagca agatcaagcc taagggcatg acacctttcg actggtacat
     2881 caacgagtgc aagaagtacg acttctacat cagcgacaac ggcgacatct ctctggatta
     2941 cggtttccct gtgatgggca agaccacctc ttattggaga gagaacatcc cgcgagagaa
     3001 gatcatctcc gttaagcaga agtgcctgcc gaacacctct gctcttacca acaggattct
     3061 gagcctttct accgtgaggg ctatccagat tgcttctgag ctggcttctg aaaagaccgt
     3121 ggttcttgct tctaggcaga ggctggatct ggacatcaag tctcagtacc ggatctcatt
     3181 ccctggtgtt caagatgagg gcgctttcac taggactttc tgcatcccta tggacaagac
     3241 cgctaggatc gtttgcttct acgctaagac ctccgtggac gtgtcaaatg agaggaccac
     3301 cttgaccatc aagatcgtca acaagaccgt cgagagcaac tacatgggtc ctgtgcctaa
     3361 ggatcacatg tactgcgata agagcatcgg cgctaggatt ggtcttgtgg atattgtgag
     3421 gggcgacccg aactacaatc agatgattgc tcgggaaatg atcagcgtcc acaccaactt
     3481 tgctctgagg ctttctgagg ctctgaagaa gccggtcatc gttttcaaga tgtacgagaa
     3541 agagctgaac ttcgagactt acgacctgtc cggtcggtct ttgtcttacc agaaggacag
     3601 cagcggcaat atctacttcc tgtctaggac ccttgagatc ctgccgaagt ctctttctac
     3661 cctgacctac ctgaagaaca tctctcctgc ctgctggaaa gaatccatca gcatgcagca
     3721 tttctacatc ggggagcaag aagagggacc ttctggttct aatctggccg agtctgaaga
     3781 gaaggtgcag actgcttagg aattcggtac gctgaaatca ccagtctctc tctacaaatc
     3841 tatctctctc tattttctcc ataaataatg tgtgagtagt ttcccgataa gggaaattag
     3901 ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
     3961 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa
     4021 aatccagatc tcctaaagtc cctatagatc tttgtcgtga atataaacca gacaccggac
     4081 gactaaacct ggagcccaga cgccgttcga agctagaagt accgcttagg caggaggccg
     4141 ttagggaaaa gatgctaagg cagggttggt tacgttgact cccccgtagg tttggtttaa
     4201 atatgatgaa gtggacggaa ggaaggagga agacaaggaa ggataaggtt gcaggccctg
     4261 tgcaaggtaa gaagatggaa atttgataga ggtacgctac tatacttata ctatacgcta
     4321 agggaatgct tgtatttata ccctataccc cctaataacc ccttatcaat ttaagaaata
     4381 atccgcataa gcccccgctt aaaaattggt atcagagcca tgaataggtc tatgaccaaa
     4441 actcaagagg ataaaacctc accaaaatac gaaagagttc ttaactctaa agataaaaga
     4501 tggcgcgtgg ccggccatga gcggagaatt aagggagtca cgttatgacc cccgccgatg
     4561 acgcgggaca agccgtttta cgtttggaac tgacagaacc gcaacgttga aggagccact
     4621 cagccgcggg tttctggagt ttaatgagct aagcacatac gtcagaaacc attattgcgc
     4681 gttcaaaagt cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa aaatgctcca
     4741 ctgacgttcc ataaattccc ctcggtatcc aattagagtc tcatattcac tctcaatcca
     4801 aataatctgc accggatctg gatcgtttcg catggtttca aagggagaag aacttttcac
     4861 cggggttgtc cctatactgg tggaactgga tggagacgtc aacgggcata agttttctgt
     4921 ctctggcgaa ggggagggtg acgcaacgta cgggaagctg acactgaagt tcatctgtac
     4981 taccggcaag ctcccggtac cctggcctac gctggtaaca acgttgactt acggggtgca
     5041 gtgtttctct cgatatcctg accatatgaa acaacacgat ttcttcaaga gtgcaatgcc
     5101 tgaggggtat gttcaagagc gaactatatt cttcaaagat gatgggaact acaagacacg
     5161 tgcagaagtt aagtttgaag gcgatacgct ggtaaaccga attgaattga aagggataga
     5221 ttttaaggaa gacgggaata tcctgggaca caaacttgaa tataactata actcccacaa
     5281 cgtttacatt atggccgata aacagaagaa tggcatcaag gtaaatttca aaattcgtca
     5341 caacatagaa gatgggtccg ttcagctcgc cgaccattat caacaaaaca ctccaatagg
     5401 ggatggcccc gtactgttgc ccgataacca ctatttgagc acgcaatcag cactttctaa
     5461 ggacccaaat gaaaaacgtg atcatatggt actcttagaa ttcgtgactg ctgctggtat
     5521 cacgcttggt atggatgaat tgtacaagtg agcgggactc tggggttcga aatgaccgac
     5581 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     5641 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     5701 atgctggagt tcttcgccca cgggatctct gcgaaacagg cggtcgaagg tgccgatatc
     5761 attacgacag caacggccga caagcacaac gccacgatcc tgagcgacaa tatgatcgcg
     5821 gcgtccacat caacggcgtc ggcggcgact gcccaggcaa gaccgagatg caccgcgata
     5881 tcttgctgcg ttcggatatt ttcgtggagt tcccgccaca gacccggatg atccccgatc
     5941 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     6001 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     6061 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     6121 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     6181 tactagatcg ggggccggcc ctcactggtg aaaagaaaaa ccaccccagt acattaaaaa
     6241 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     6301 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     6361 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
     6421 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
     6481 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
     6541 caaatatcat ctccctcgca gagatccgaa ttatcagcct tcttattcat ttctcgctta
     6601 accgtgacag agtagacagg ctgtctcgcg gccgaggggc gcagcccctg gggggatggg
     6661 aggcccgcgt tagcgggccg ggagggttcg agaagggggg gcacccccct tcggcgtgcg
     6721 cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata ttggtttaaa
     6781 agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct tgcaaatgct
     6841 ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc atctgtcagc
     6901 actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg cgcccctcaa
     6961 gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg ggaaactcgc
     7021 gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg ccggccgaaa
     7081 tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc aagtgtcaac
     7141 gtccgcccct catctgtcag tgagggccaa gttttccgcg aggtatccac aacgccggcg
     7201 gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag ggccatagac
     7261 ggccgccagc ccagcggcga gggcaaccag cccggtgagc gtcggaaagg cgctcggtct
     7321 tgccttgctc gtcggtgatg tacactagtc gctggctgct gaacccccag ccggaactga
     7381 ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac
     7441 caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac
     7501 gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc
     7561 ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt
     7621 ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat
     7681 caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag
     7741 cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag
     7801 gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc
     7861 ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta
     7921 ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta
     7981 ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg
     8041 gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg
     8101 catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg
     8161 cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc
     8221 gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt
     8281 cgccaaacct gccgcctcct gttcaagacg acgcgaacgc tccacggcgg ccgatggcgc
     8341 gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg
     8401 gaccatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc
     8461 gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt
     8521 ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc
     8581 aatgtgccct tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt
     8641 atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg
     8701 aatcttgccc tgcacgaata ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc
     8761 ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc
     8821 gttgcgccac atctaggtac taaaacaatt catccagtaa aatataatat tttattttct
     8881 cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga
     8941 tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc
     9001 ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc
     9061 ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat
     9121 acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg
     9181 ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat
     9241 agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga
     9301 taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct
     9361 cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag
     9421 gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc
     9481 ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat
     9541 ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt
     9601 ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca
     9661 gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct
     9721 tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc
     9781 gtataacata gtatcgacgg agccgatttt gaaaccacaa ttatgggtga tgctgccaac
     9841 ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag
     9901 ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca
     9961 tcagcgctat ctctgctctc actgccgtaa aacatggcaa ctgcagttca cttacaccgc
    10021 ttctcaaccc ggtacgcacc agaaaatcat tgatatggcc atgaatggcg ttggatgccg
    10081 ggcaacagcc cgcattatgg gcgttggcct caacacgatt ttacgtcact taaaaaactc
    10141 aggccgcagt cggtaactat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
    10201 cgcatcaggc gctattccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    10261 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    10321 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    10381 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    10441 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    10501 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    10561 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    10621 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    10681 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    10741 gcagcaggta acctcgcgca tacagccggg cagtgacgtc atcgtctgcg cggaaatgga
    10801 cgggcccccg gcgccagatc tggggaaccc tgtggttggc atgcacatac aaatggacga
    10861 acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac gctcttttct
    10921 cttaggttta cccgccaata tatcctgtca aacactgata gtttgtgaac catcacccaa
    10981 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
    11041 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
    11101 gaaaggagcg ggcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    11161 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    11221 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg ttagagggag
    11281 ctctgatcac tgagcatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc
    11341 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
    11401 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcttaa
    11461 ttaaggaaac ctcctcggat tccattgccc agctatctgt cactttattg agaagatagt
    11521 ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ccatcgttga
    11581 agatgcctct gccgacagtg gtcccaaaga tggaccccca ccca
//
