LOCUS       sequence-490473-       11704 bp    DNA     circular SYN 23-OCT-2025
DEFINITION  Targets high levels of recombinant protein to chloroplast. Empty 
            vector. Clone via SapI restriction site. mCherry dropout cassette 
            will enable Red/White screening in E.coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11704)
  AUTHORS   Chartrand-Pleau C, Gallipeau-Burns E, Jain A, Boddy V, Fakhouri P, 
            Rehmani S, Thomas T, Drapeau M, VanderBurgt JT, Boudigou A, Damry 
            AM, MacLean AM
  TITLE     pTARGEX Vectors: A Versatile Toolbox for Plant-Based Protein 
            Expression
  JOURNAL   Front. Synth. Biol. 3:1619871
REFERENCE   2  (bases 1 to 11704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front. 
            Synth. Biol. 3:1619871"
COMMENT     Sequence Label: pTARGEX-CHL
FEATURES             Location/Qualifiers
     source          1..11704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        110..155
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    514..535
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(550..555,556..573,574..580)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    588..604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             644..666
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      1454..1481
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1716..1968
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2116..2461
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3934..4110
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4676..4855
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4708..4891
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(4912..4914,4915..4917,4918..5628)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      6017..6269
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6360..6384
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      6752..7383
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(7473..8621)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8920..9714)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     complement(10304..10321)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10555..10574)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11006..11030
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     11169..11188
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11313..11335
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11327..11344
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11328..11344
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
       61 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
      121 cctctatata aggaagttca tttcatttgg agaggtttaa gagacgcaac cacaacgctc
      181 taacgcaatc aatctacatt atattaaacg tctctaaaaa gaaaccatgg cttcctcagt
      241 tctttcctct gcagcagttg ccacccgcag caatgttgct caagctaaca tggttgcacc
      301 tttcactggc cttaagtcag ctgcctcatt ccctgtttca aggaagcaaa accttgacat
      361 cacttccatt gccagcaacg gcggaagagt gcaatgcggc tgaagagcat caacaatacg
      421 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
      481 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
      541 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
      601 acaatttcac acaagaccac aacggtttcc ctctagaaat aattttgttt aactttaaga
      661 aggagatata catatggttt ccaagggaga ggaagacaat atggcgatta tcaaagagtt
      721 catgcgcttc aaggtccaca tggaaggaag tgtgaacggg catgagttcg aaatagaagg
      781 cgaaggtgag ggtcgcccgt acgaaggtac gcaaaccgca aagctgaagg ttactaaggg
      841 tgggcctctg ccgttcgcat gggacatctt atctccgcaa ttcatgtacg gtagtaaggc
      901 ttacgtaaag caccccgccg atattcccga ctacttgaag ttaagtttcc cagaggggtt
      961 caagtgggag agagtaatga atttcgagga cggcggggta gtcaccgtaa cccaagactc
     1021 atctcttcaa gacggtgagt ttatatacaa ggtgaagctg cgcgggacga acttcccatc
     1081 tgacggacca gtgatgcaga agaagactat gggatgggaa gccagtagcg aaagaatgta
     1141 cccagaagac ggggccctta aaggagaaat taagcagcgg ctcaagttga aggatggcgg
     1201 gcactatgat gcagaggtca aaaccaccta caaggcaaag aaaccagtac aattgcccgg
     1261 tgcgtacaac gtaaacatta aattagacat cacttctcat aatgaggact acacaatagt
     1321 ggagcaatac gagcgagcag aggggagaca ctctacgggt ggtatggacg agttgtataa
     1381 ataagaattc gaagcttgat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     1441 ctgccatcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagagaga
     1501 atataaaaag ccagattatt aatccggctt ttttattatt tgagttgctc ttcatgatga
     1561 gtataaataa actgttattc ggtgtgcatt tctatgtttg gtgagcggtt ttctgtgctc
     1621 agagtgtgtt tattttatgt aatttaattt ctttgtgagc tcctgtttag caggtcgtcc
     1681 cttcagcaag gacacaaaaa gattttaatt ttattgatcg ttcaaacatt tggcaataaa
     1741 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     1801 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     1861 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     1921 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg cgcgccagct
     1981 tggcgtaatc atggtcatag ctgttgcgat taataattcc acgcggatcg cattgagaac
     2041 ccggaatatc gcgaaacagg taccccccta ctccaaaaat gtcaaagata cagtctcaga
     2101 agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc tcctcggatt
     2161 ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta
     2221 caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg
     2281 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
     2341 gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc
     2401 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
     2461 agcccaagct tcgactctag aggatcccct taaatcgata tgagcaccgc caagatgagc
     2521 gctggtgagt tctctaagac ctacggcacc aaggatagcc ggtctgtgaa tgattgctac
     2581 gccatctaca gcggcaacgg catcaacttc atcaacctgt tcatgcacac caacaccggc
     2641 atcaagagcg ccttctctat taacgacctt ggccggaacg aggacattaa gcttcatgag
     2701 gccgagatca tcaacagctt gcacccgtac tcattcttcg acaagttcgg cctggatatc
     2761 atcctgtgca accacgtgat ggacatcatc gtgactaagc ctggccttaa gaacaccgga
     2821 tgcaagttcc agatgcacaa ccagatcttc aacccgaatg aggatattct ggctaagacc
     2881 cctggcatca tcagcgaaga gaacttctac gacctgagca agatcaagcc taagggcatg
     2941 acacctttcg actggtacat caacgagtgc aagaagtacg acttctacat cagcgacaac
     3001 ggcgacatct ctctggatta cggtttccct gtgatgggca agaccacctc ttattggaga
     3061 gagaacatcc cgcgagagaa gatcatctcc gttaagcaga agtgcctgcc gaacacctct
     3121 gctcttacca acaggattct gagcctttct accgtgaggg ctatccagat tgcttctgag
     3181 ctggcttctg aaaagaccgt ggttcttgct tctaggcaga ggctggatct ggacatcaag
     3241 tctcagtacc ggatctcatt ccctggtgtt caagatgagg gcgctttcac taggactttc
     3301 tgcatcccta tggacaagac cgctaggatc gtttgcttct acgctaagac ctccgtggac
     3361 gtgtcaaatg agaggaccac cttgaccatc aagatcgtca acaagaccgt cgagagcaac
     3421 tacatgggtc ctgtgcctaa ggatcacatg tactgcgata agagcatcgg cgctaggatt
     3481 ggtcttgtgg atattgtgag gggcgacccg aactacaatc agatgattgc tcgggaaatg
     3541 atcagcgtcc acaccaactt tgctctgagg ctttctgagg ctctgaagaa gccggtcatc
     3601 gttttcaaga tgtacgagaa agagctgaac ttcgagactt acgacctgtc cggtcggtct
     3661 ttgtcttacc agaaggacag cagcggcaat atctacttcc tgtctaggac ccttgagatc
     3721 ctgccgaagt ctctttctac cctgacctac ctgaagaaca tctctcctgc ctgctggaaa
     3781 gaatccatca gcatgcagca tttctacatc ggggagcaag aagagggacc ttctggttct
     3841 aatctggccg agtctgaaga gaaggtgcag actgcttagg aattcggtac gctgaaatca
     3901 ccagtctctc tctacaaatc tatctctctc tattttctcc ataaataatg tgtgagtagt
     3961 ttcccgataa gggaaattag ggttcttata gggtttcgct catgtgttga gcatataaga
     4021 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     4081 aaaccaaaat ccagtactaa aatccagatc tcctaaagtc cctatagatc tttgtcgtga
     4141 atataaacca gacaccggac gactaaacct ggagcccaga cgccgttcga agctagaagt
     4201 accgcttagg caggaggccg ttagggaaaa gatgctaagg cagggttggt tacgttgact
     4261 cccccgtagg tttggtttaa atatgatgaa gtggacggaa ggaaggagga agacaaggaa
     4321 ggataaggtt gcaggccctg tgcaaggtaa gaagatggaa atttgataga ggtacgctac
     4381 tatacttata ctatacgcta agggaatgct tgtatttata ccctataccc cctaataacc
     4441 ccttatcaat ttaagaaata atccgcataa gcccccgctt aaaaattggt atcagagcca
     4501 tgaataggtc tatgaccaaa actcaagagg ataaaacctc accaaaatac gaaagagttc
     4561 ttaactctaa agataaaaga tggcgcgtgg ccggccatga gcggagaatt aagggagtca
     4621 cgttatgacc cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacagaacc
     4681 gcaacgttga aggagccact cagccgcggg tttctggagt ttaatgagct aagcacatac
     4741 gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc tagcaaatat
     4801 ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc
     4861 tcatattcac tctcaatcca aataatctgc accggatctg gatcgtttcg catggtttca
     4921 aagggagaag aacttttcac cggggttgtc cctatactgg tggaactgga tggagacgtc
     4981 aacgggcata agttttctgt ctctggcgaa ggggagggtg acgcaacgta cgggaagctg
     5041 acactgaagt tcatctgtac taccggcaag ctcccggtac cctggcctac gctggtaaca
     5101 acgttgactt acggggtgca gtgtttctct cgatatcctg accatatgaa acaacacgat
     5161 ttcttcaaga gtgcaatgcc tgaggggtat gttcaagagc gaactatatt cttcaaagat
     5221 gatgggaact acaagacacg tgcagaagtt aagtttgaag gcgatacgct ggtaaaccga
     5281 attgaattga aagggataga ttttaaggaa gacgggaata tcctgggaca caaacttgaa
     5341 tataactata actcccacaa cgtttacatt atggccgata aacagaagaa tggcatcaag
     5401 gtaaatttca aaattcgtca caacatagaa gatgggtccg ttcagctcgc cgaccattat
     5461 caacaaaaca ctccaatagg ggatggcccc gtactgttgc ccgataacca ctatttgagc
     5521 acgcaatcag cactttctaa ggacccaaat gaaaaacgtg atcatatggt actcttagaa
     5581 ttcgtgactg ctgctggtat cacgcttggt atggatgaat tgtacaagtg agcgggactc
     5641 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     5701 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     5761 tcctccagcg cggggatctc atgctggagt tcttcgccca cgggatctct gcgaaacagg
     5821 cggtcgaagg tgccgatatc attacgacag caacggccga caagcacaac gccacgatcc
     5881 tgagcgacaa tatgatcgcg gcgtccacat caacggcgtc ggcggcgact gcccaggcaa
     5941 gaccgagatg caccgcgata tcttgctgcg ttcggatatt ttcgtggagt tcccgccaca
     6001 gacccggatg atccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     6061 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     6121 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     6181 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     6241 gcgcgcggtg tcatctatgt tactagatcg ggggccggcc ctcactggtg aaaagaaaaa
     6301 ccaccccagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
     6361 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
     6421 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
     6481 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
     6541 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
     6601 agagcgttgc tgcctgtgat caaatatcat ctccctcgca gagatccgaa ttatcagcct
     6661 tcttattcat ttctcgctta accgtgacag agtagacagg ctgtctcgcg gccgaggggc
     6721 gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg
     6781 gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg
     6841 tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg
     6901 cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg
     6961 tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg
     7021 tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac
     7081 atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag
     7141 ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag
     7201 tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg
     7261 aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc
     7321 gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag cccggtgagc
     7381 gtcggaaagg cgctcggtct tgccttgctc gtcggtgatg tacactagtc gctggctgct
     7441 gaacccccag ccggaactga ccccacaagg ccctagcgtt tgcaatgcac caggtcatca
     7501 ttgacccagg cgtgttccac caggccgctg cctcgcaact cttcgcaggc ttcgccgacc
     7561 tgctcgcgcc acttcttcac gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc
     7621 agcttgagcg ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc
     7681 ggcgacagct tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc
     7741 acgacgattt cctcgtcgat caggacctgg caacgggacg ttttcttgcc acggtccagg
     7801 acgcggaagc ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag
     7861 cccatcgccg tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta ataccggcca
     7921 ttgatcgacc agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg
     7981 ataggggtgc gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc
     8041 cgcagctcga cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg
     8101 ttttgcagcg cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc
     8161 gtgtcgtttg gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa caaggaaagc
     8221 tgcatttcct tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg
     8281 acctgttttg ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat agttcctcgc
     8341 gtgtcgatgg tcatcgactt cgccaaacct gccgcctcct gttcaagacg acgcgaacgc
     8401 tccacggcgg ccgatggcgc gggcagggca gggggagcca gttgcacgct gtcgcgctcg
     8461 atcttggccg tagcttgctg gaccatcgag ccgacggact ggaaggtttc gcggggcgca
     8521 cgcatgacgg tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc
     8581 agttcttgcc tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt
     8641 gccccgactc acgccggggc aatgtgccct tattcctgat ttgacccgcc tggtgccttg
     8701 gtgtccagat aatccacctt atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc
     8761 ttctcgtact tggtattccg aatcttgccc tgcacgaata ccagcgaccc cttgcccaaa
     8821 tacttgccgt gggcctcggc ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc
     8881 tcctgcttgt cgccggcatc gttgcgccac atctaggtac taaaacaatt catccagtaa
     8941 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
     9001 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
     9061 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
     9121 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
     9181 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
     9241 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
     9301 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
     9361 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
     9421 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
     9481 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
     9541 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
     9601 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
     9661 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
     9721 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
     9781 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
     9841 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccacaa
     9901 ttatgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca
     9961 gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac
    10021 ggcaaaagca ccgccggaca tcagcgctat ctctgctctc actgccgtaa aacatggcaa
    10081 ctgcagttca cttacaccgc ttctcaaccc ggtacgcacc agaaaatcat tgatatggcc
    10141 atgaatggcg ttggatgccg ggcaacagcc cgcattatgg gcgttggcct caacacgatt
    10201 ttacgtcact taaaaaactc aggccgcagt cggtaactat gcggtgtgaa ataccgcaca
    10261 gatgcgtaag gagaaaatac cgcatcaggc gctattccgc ttcctcgctc actgactcgc
    10321 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    10381 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    10441 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    10501 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    10561 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    10621 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    10681 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    10741 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    10801 gacacgactt atcgccactg gcagcaggta acctcgcgca tacagccggg cagtgacgtc
    10861 atcgtctgcg cggaaatgga cgggcccccg gcgccagatc tggggaaccc tgtggttggc
    10921 atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat
    10981 tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata
    11041 gtttgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
    11101 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
    11161 agaaaggaag ggaagaaagc gaaaggagcg ggcgccattc aggctgcgca actgttggga
    11221 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    11281 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    11341 cagtgaattg ttagagggag ctctgatcac tgagcatcgt tgaagatgcc tctgccgaca
    11401 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
    11461 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
    11521 aatcccacta tccttcttaa ttaaggaaac ctcctcggat tccattgccc agctatctgt
    11581 cactttattg agaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
    11641 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
    11701 ccca
//
