LOCUS       sequence-490474-        6788 bp    DNA     circular SYN 23-OCT-2025
DEFINITION  Mammalian expression of C-terminal HA-tagged rat CAPS1 379-1289aa 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6788)
  TITLE     Martin lab plasmids 2025 (Yamaga)
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV-CAPS1(379-1289)-HA
FEATURES             Location/Qualifiers
     source          1..6788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     614..630
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     615..631
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      631..640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3424..3450
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3576..3697
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3613..3632)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3667..3686
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3704..4159)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3841..3862)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4053..4072
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4186..4290
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4292..4649
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4292..4483
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4315..4335)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4500..4635
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4684..5478
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4684..5478
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4738..4757)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5348..5367
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5666..5685)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5710..5757
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6086..6674
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6575..6594
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggt cgacggtatc gccaccatgg gtgaggagag cgagaaccag
      661 ctgtccaagt cggatgtcct gctgtccttc tccttggagg ttgtcatcat ggaagtccaa
      721 ggccttaagt ccttggctcc aaaccgaatc gtgtactgca caatggaggt ggagggtgga
      781 gagaaacttc agactgatca agcagaggct tctaaaccaa cctggggcac tcaaggcgac
      841 ttctctacaa cccacgcgct gccagctgtt aaagtaaagc tattcacaga gagcactggt
      901 gtcctggcct tggaagacaa ggaactcggg cgggttatcc tccatcccac cccaaacagc
      961 cccaaacaat ccgagtggca caaaatgaca gtctccaaaa actgccccga tcaagacctc
     1021 aaaatcaaac tcgctgtccg aatggataag cctcaaaaca tgaagcactc tgggtactta
     1081 tggaccattg gtaagaatgt gtggaagagg tggaagaaaa ggttctttgt gttggtgcag
     1141 gtcagccagt acacttttgc catgtgcagc tatcgagaaa agaaagcaga acctcaggaa
     1201 ctcctacagc tggatggcta caccgtggat tacaccgacc ctcagccagg tttggagggt
     1261 ggccgagcct tcttcaatgc agtcaaagaa ggagacacgg tgatatttgc cagtgacgat
     1321 gagcaagatc gaatcctgtg ggtccaagct atgtacaggg ccaccgggca gtcgcacaaa
     1381 cctgtgcccc ctacccaggt gcagaagctc aacgccaagg gcggaaacgt gcctcagctg
     1441 gatgccccca tctcccagtt ttacgcagat agagctcaaa agcatggcat ggatgaattt
     1501 atctcgtcca acccctgtaa ctttgaccac gcttccctct ttgagatggt acagcggctg
     1561 actttggatc acagactaaa cgattcctat tcttgcctgg gctggttcag tcctggccag
     1621 gtgtttgtgt tagacgagta ctgcgctcgg aatggagtcc gaggatgtca tcggcatctc
     1681 tgttacctta gagacttgct ggaacgggca gaaaatggcg ccatgatcga ccccacgctt
     1741 cttcattaca gctttgcctt ctgcgcatcc catgtccacg ggaacaggcc tgatggaatc
     1801 ggaactgtga ctgttgaaga aaaagagcgc tttgaagaaa tcaaagagag actccgagtt
     1861 ctgctggaaa atcaaattac acatttcagg tactgctttc cgtttggtcg acctgaaggt
     1921 gctttgaaag cgactctctc acttttggaa cgggttttga tgaaagatat tgtcactcca
     1981 gtaccccaag aggaggtcaa aactgtcatc cgtaaatgcc tagagcaggc tgctctagtc
     2041 aactactctc gcctatctga gtatgccaaa atcgaagaaa atgtaggccg cttaatcact
     2101 cccgccaaaa agctcgaaga cacaatccgt cttgctgaac tagtcattga agtgctgcaa
     2161 caaaacgaag agcaccacgc cgaggccttt gcgtggtggt cagacttaat ggtggagcac
     2221 gcggagacgt tcctgtcact ctttgcagtg gacatggatg cagcattaga ggtgcaaccc
     2281 ccagacacgt gggacagttt cccactcttc cagctgctga atgactttct ccgtacagac
     2341 tataacttgt gcaatggaaa gtttcacaaa cacctgcagg atctgttcgc cccactcgtt
     2401 gttagatacg tggatctgat ggagtcctca attgcacaat ctatccacag gggctttgag
     2461 cgggagtcat gggaaccagt caataacgga tcgggtacat cagaagattt gttctggaaa
     2521 cttgatgccc ttcagacttt catccgtgac ctacactggc ctgaggaaga gtttggaaag
     2581 cacttagagc agcggctaaa actaatggca agcgacatga ttgagtcctg cgtcaagaga
     2641 accaggattg catttgaagt taagctgcaa aaaaccagtc gatcaacaga ttttcgagtc
     2701 ccacagtcaa tatgcaccat gtttaatgtt atggttgatg ccaaagctca atcaacaaaa
     2761 ctttgcagca tggaaatggg ccaggagcat caataccatt caaaaataga cgaactaatt
     2821 gaagaaaccg ttaaggaaat gatcacactc ttggtggcaa agtttgtaac tatattggaa
     2881 ggcgtactgg caaagttatc cagatacgac gaggggactt tgttttcttc gtttctgtca
     2941 tttacggtga aggcggcatc taagtatgtg gacgtaccta aacccgggat ggatgtggcc
     3001 gacgcctacg tgactttcgt ccgccactct caggatgtcc ttcgtgataa ggtcaatgag
     3061 gagatgtata tagaaaggtt atttgatcaa tggtacaaca gctccatgaa cgtcatctgc
     3121 acctggctga ccgaccggat ggacctccag cttcacattt atcagctgaa aacactaatt
     3181 aggatggtaa agaaaaccta cagagatttc cgattgcaag gggtcctgga ttccacctta
     3241 aacagcaaga cctatgagac cattcgaaat cggctcactg tagaggaagc taccgcgtcc
     3301 gtgagtgagg gagggggctt gcaaggcatc agcatgaagg acagcgatga agaggacgag
     3361 gaggatgacg gtggaggagg ttctggccac cacactggac tagtggatcc accggtcgcc
     3421 acctacccat acgatgttcc agattacgct taaagcggcc gcgactctag atcataatca
     3481 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     3541 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     3601 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3661 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat
     3721 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     3781 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     3841 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     3901 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     3961 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     4021 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     4081 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     4141 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4201 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4261 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
     4321 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4381 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4441 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4501 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4561 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     4621 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
     4681 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     4741 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     4801 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     4861 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     4921 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     4981 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     5041 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     5101 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     5161 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     5221 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     5281 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     5341 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     5401 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     5461 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     5521 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     5581 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     5641 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     5701 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
     5761 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     5821 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     5881 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
     5941 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6001 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6061 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6121 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6181 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6241 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6301 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6361 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6421 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6481 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6541 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6601 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6661 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6721 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6781 ccatgcat
//
