LOCUS       sequence-490475-        7922 bp    DNA     circular SYN 23-OCT-2025
DEFINITION  Mammalian expression of C-terminal HA-tagged rat CAPS1 R944L mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7922)
  TITLE     Martin lab plasmids 2025 (Yamaga)
REFERENCE   2  (bases 1 to 7922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV-CAPS1(R944L)-HA
FEATURES             Location/Qualifiers
     source          1..7922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     614..630
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     615..631
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4558..4584
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4710..4831
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4747..4766)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4801..4820
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4838..5293)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4975..4996)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5187..5206
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5320..5424
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5426..5783
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5426..5617
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5449..5469)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5634..5769
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5818..6612
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5818..6612
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5872..5891)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6482..6501
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6800..6819)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6844..6891
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7220..7808
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7709..7728
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggt cgacggtatc gccaccatgc tggacccttc gtccagcgaa
      661 gaggaatcgg atgagatcct ggaagaggag agcggcaagg aggtgctggg ctcggccgcg
      721 tccggagcgc gcctgtctcc aagccgcacc aacgagggct cggcgggcag cgccggcatg
      781 gggggcagtg gcgccggggc cggggtgggc gccggcggcg gtgggggtag cggcgcgagc
      841 agcggcggcg gggccggggg gctgcagccc agcagccgcg ccggcggcgg ccggccctcc
      901 agccccagcc cgtcggtggt gagcgagaag gagaaggaag agttggagcg gctgcagaag
      961 gaagaggagg agaggaagaa gaggctgcag ctgtacgtgt tcgtgatgcg ctgcattgcc
     1021 taccccttca acgccaagca gcccaccgac atggctcggc ggcagcagaa gatcagcaaa
     1081 cagcagctgc agactgtcaa ggaccgattc caggctttcc tcaatgggga gacccaaatc
     1141 gtggcagatg aagcctttat gaatgctgtc cagagctact atgaggtgtt tctcaagagt
     1201 gaccgtgtgg cccgcatggt acagagtggg ggctgctcag ccaacgactc tcgggaggtc
     1261 ttcaagaaac acatcgagaa gagggtacgc agcctacctg agattgatgg cctcagcaag
     1321 gagactgtgc taagctcctg gatggctaag tttgacgcca tctaccgtgg tgaggaggac
     1381 cccaggaagc agcaagcccg gatgacagcc agtgcagctt ctgagttgat tctgagcaag
     1441 gaacagctct atgagatgtt ccagaacatt cttgggatca agaaatttga acatcaactc
     1501 ctatataatg cgtgtcagct ggacaatcca gatgagcagg cagcccagat cagacgagag
     1561 ctggacggac gtctccaaat ggcagaccaa atagccaggg aacgcaaatt tcctaagttc
     1621 gtatccaaag aaatggaaaa tatgtacatc gaggagctga agtcctccgt caacctactc
     1681 atggccaact tggagagcat gccggtgtcc aagggtggag agttcaagct gcagaaactg
     1741 aaacgcagtc acaacgcgtc catcattgac atgggtgagg agagcgagaa ccagctgtcc
     1801 aagtcggatg tcctgctgtc cttctccttg gaggttgtca tcatggaagt ccaaggcctt
     1861 aagtccttgg ctccaaaccg aatcgtgtac tgcacaatgg aggtggaggg tggagagaaa
     1921 cttcagactg atcaagcaga ggcttctaaa ccaacctggg gcactcaagg cgacttctct
     1981 acaacccacg cgctgccagc tgttaaagta aagctattca cagagagcac tggtgtcctg
     2041 gccttggaag acaaggaact cgggcgggtt atcctccatc ccaccccaaa cagccccaaa
     2101 caatccgagt ggcacaaaat gacagtctcc aaaaactgcc ccgatcaaga cctcaaaatc
     2161 aaactcgctg tccgaatgga taagcctcaa aacatgaagc actctgggta cttatggacc
     2221 attggtaaga atgtgtggaa gaggtggaag aaaaggttct ttgtgttggt gcaggtcagc
     2281 cagtacactt ttgccatgtg cagctatcga gaaaagaaag cagaacctca ggaactccta
     2341 cagctggatg gctacaccgt ggattacacc gaccctcagc caggtttgga gggtggccga
     2401 gccttcttca atgcagtcaa agaaggagac acggtgatat ttgccagtga cgatgagcaa
     2461 gatcgaatcc tgtgggtcca agctatgtac agggccaccg ggcagtcgca caaacctgtg
     2521 ccccctaccc aggtgcagaa gctcaacgcc aagggcggaa acgtgcctca gctggatgcc
     2581 cccatctccc agttttacgc agatagagct caaaagcatg gcatggatga atttatctcg
     2641 tccaacccct gtaactttga ccacgcttcc ctctttgaga tggtacagcg gctgactttg
     2701 gatcacagac taaacgattc ctattcttgc ctgggctggt tcagtcctgg ccaggtgttt
     2761 gtgttagacg agtactgcgc tcggaatgga gtccgaggat gtcatcggca tctctgttac
     2821 cttagagact tgctggaacg ggcagaaaat ggcgccatga tcgaccccac gcttcttcat
     2881 tacagctttg ccttctgcgc atcccatgtc cacgggaaca ggcctgatgg aatcggaact
     2941 gtgactgttg aagaaaaaga gcgctttgaa gaaatcaaag agagactccg agttctgctg
     3001 gaaaatcaaa ttacacattt caggtactgc tttccgtttg gtcgacctga aggtgctttg
     3061 aaagcgactc tctcactttt ggaacgggtt ttgatgaaag atattgtcac tccagtaccc
     3121 caagaggagg tcaaaactgt catccgtaaa tgcctagagc aggctgctct agtcaactac
     3181 tctcgcctat ctgagtatgc caaaatcgaa gaaaatgtag gccgcttaat cactcccgcc
     3241 aaaaagctcg aagacacaat ccgtcttgct gaactagtca ttgaagtgct gcaacaaaac
     3301 gaagagcacc acgccgaggc ctttgcgtgg tggtcagact taatggtgga gcacgcggag
     3361 acgttcctgt cactctttgc agtggacatg gatgcagcat tagaggtgca acccccagac
     3421 acgtgggaca gtttcccact cttccagctg ctgaatgact ttctccttac agactataac
     3481 ttgtgcaatg gaaagtttca caaacacctg caggatctgt tcgccccact cgttgttaga
     3541 tacgtggatc tgatggagtc ctcaattgca caatctatcc acaggggctt tgagcgggag
     3601 tcatgggaac cagtcaataa cggatcgggt acatcagaag atttgttctg gaaacttgat
     3661 gcccttcaga ctttcatccg tgacctacac tggcctgagg aagagtttgg aaagcactta
     3721 gagcagcggc taaaactaat ggcaagcgac atgattgagt cctgcgtcaa gagaaccagg
     3781 attgcatttg aagttaagct gcaaaaaacc agtcgatcaa cagattttcg agtcccacag
     3841 tcaatatgca ccatgtttaa tgttatggtt gatgccaaag ctcaatcaac aaaactttgc
     3901 agcatggaaa tgggccagga gcatcaatac cattcaaaaa tagacgaact aattgaagaa
     3961 accgttaagg aaatgatcac actcttggtg gcaaagtttg taactatatt ggaaggcgta
     4021 ctggcaaagt tatccagata cgacgagggg actttgtttt cttcgtttct gtcatttacg
     4081 gtgaaggcgg catctaagta tgtggacgta cctaaacccg ggatggatgt ggccgacgcc
     4141 tacgtgactt tcgtccgcca ctctcaggat gtccttcgtg ataaggtcaa tgaggagatg
     4201 tatatagaaa ggttatttga tcaatggtac aacagctcca tgaacgtcat ctgcacctgg
     4261 ctgaccgacc ggatggacct ccagcttcac atttatcagc tgaaaacact aattaggatg
     4321 gtaaagaaaa cctacagaga tttccgattg caaggggtcc tggattccac cttaaacagc
     4381 aagacctatg agaccattcg aaatcggctc actgtagagg aagctaccgc gtccgtgagt
     4441 gagggagggg gcttgcaagg catcagcatg aaggacagcg atgaagagga cgaggaggat
     4501 gacggtggag gaggttctgg ccaccacact ggactagtgg atccaccggt cgccacctac
     4561 ccatacgatg ttccagatta cgcttaaagc ggccgcgact ctagatcata atcagccata
     4621 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     4681 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     4741 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4801 gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg
     4861 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     4921 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     4981 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     5041 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     5101 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     5161 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     5221 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     5281 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5341 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5401 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     5461 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5521 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5581 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5641 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5701 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     5761 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
     5821 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     5881 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5941 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     6001 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     6061 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     6121 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     6181 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     6241 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     6301 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     6361 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     6421 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     6481 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     6541 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     6601 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     6661 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     6721 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     6781 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     6841 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
     6901 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     6961 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     7021 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
     7081 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7141 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7201 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7261 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7321 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7381 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7441 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7501 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7561 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7621 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7681 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7741 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7801 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7861 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
     7921 at
//
