LOCUS       sequence-490483-        2275 bp    DNA     circular SYN 23-OCT-2025
DEFINITION  Express ISCro4 bridge RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Perry NT, Bartie LJ, Katrekar D, Gonzalez GA, Durrant MG, Pai JJ, 
            Fanton A, Martins JQ, Hiraizumi M, Ricci-Tam C, Nishimasu H, 
            Konermann S, Hsu PD
  TITLE     Megabase-scale human genome rearrangement with programmable bridge 
            recombinases.
  JOURNAL   Science. 2025 Sep 25:eadz0276. doi: 10.1126/science.adz0276.
  PUBMED    40997214
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2025 Sep 25:eadz0276. doi: 10.1126/science.adz0276."
COMMENT     Sequence Label: pNP3620 [pNP2916 - ISCro4 bRNA (TBL2, WT + DBL...
FEATURES             Location/Qualifiers
     source          1..2275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        489..593
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(594..662,663..1454)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(812..831)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1625..2213
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2114..2133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg agtgcaggga gaaccggcca gttctctctg ccatgcggtc cgcatgccgt
      301 atcaggcctc aggctaataa cctgtgacgt agatttatgc agcggaccgc cgttctccac
      361 aagtgacaaa ccggacagta tcatggaccg gttttcccgg taatccgcat tcacaaggct
      421 ggtctcactt tttatttttt tgttctagtc cgtttttgac gtcaggtggc acttttcggg
      481 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      541 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      601 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      661 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      721 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      781 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      841 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
      901 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
      961 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1021 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1081 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1141 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1201 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1261 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtgga agccgcggta
     1321 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1381 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1441 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1501 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1561 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1621 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1681 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1741 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1801 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1861 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1921 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1981 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2041 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2101 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2161 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2221 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcag ctagc
//
