LOCUS       sequence-490484-       10087 bp    DNA     circular SYN 23-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 10087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-OTUD4
FEATURES             Location/Qualifiers
     source          1..10087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(254..670)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(270..286)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(302..324)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    737..1078
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             1142..1658
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(1858..1877)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2005..2027)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2221..2240)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2340..2362
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2400..2418)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2486..2590
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2591..2659,2660..3451)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2809..2828)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3622..4210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4111..4130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4364..4381
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4498..4519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4534..4539,4540..4557,4558..4564)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4572..4588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(5174..5773)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5257..5277)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5754..5773
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5794..6293)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5842..5861)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6235..6258
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    6312..6336
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7422..7439)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    complement(9484..9508)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(9524..9550)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(9563..9586)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      9586..9595
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9668..9692)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    9702..9720
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9723..9741
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(9743..9946)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9772..9792)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        join(9947..10087,1..239)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
       61 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
      121 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
      181 gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca
      241 aggatctaat tccggcgcct agagaaggag tgagggctgg ataaagggag gatcgaggcg
      301 gggtcgaacg aggaggttca agggggagag acggggcgga tggaggaaga ggaggcggag
      361 gcttagggtg tacaaagggc ttgacccagg gaggggggtc aaaagccaag gcttcccagg
      421 tcacgatgta ggggacctgg tctgggtgtc catgcgggcc aggtgaaaag accttgatct
      481 taacctgggt gatgaggtct cggttaaagg tgccgtctcg cggccatccg acgttaaagg
      541 ttggccattc tgcagagcag aaggtaaccc aacgtctctt cttgacatct accgactggt
      601 tgtgagcgat ccgctcgaca tctttccagt gacctaaggt caaacttaag ggagtggtaa
      661 cagtctggcc ctaattttca gacaaataca gaaacacagt cagacagaga caacacagaa
      721 cgatgctgca gcgctgcagc agacaagacg cgcggcttcg gttccaaacc gaaagcaaaa
      781 attcagacgg aggcgggaac tgttttaggt tctcgtctcc taccagaacc acatatcctg
      841 acggggtcgg attccacatc gactcccttc ctcaggtcgg gccacaaaaa cggcccccaa
      901 agtccctggg acgtctccca gggttgcggc cgggtgttca gaactcgtca gttccaccac
      961 gggtccgcca gatacagagc tagttagcta actagtacag acgcaggcgc aaacattaga
     1021 tgccggcaca aacacgcaca aagacagaga cagacacgaa acgaccgctg gccagcttac
     1081 ctcccggcgg gggggtcggt ggtccctggg caggggtctc caaatctcgg tggaacctcc
     1141 aaatgaaaga cccccgaggt gggcagtcaa tcaatctgag gagaccctcc caaggatcag
     1201 cgagtccacg attcggatgc aaacagcaag aggctttatt gggaatacgg gtacccgggc
     1261 gacgcagtct atcggaggac tggcgcgccg agtgaggggt tgtgggctct tttattgagc
     1321 tcggggagca gaagcgcgcg aacagaagcg agaagcgaac tgattggtta gttcaaataa
     1381 ggcacagggt catttcaggt ccttggggca ccctggaaac atctgatggt tctctagaaa
     1441 ctgctgaggg cgggaccgca tctggggacc atctgttctt ggccctgagc cggggcagga
     1501 actgcttacc acagatatcc tgtttggccc atattctgct gtctctctgt tcctaacctt
     1561 gatctgaact tctctattct cagttatgta ttttccatgc cttgcaaaat ggcgttactt
     1621 aagctagctt gccaaaccta caggtggggt ctttcattcc cccctttttc tggagactaa
     1681 ataaaatctt ttattttatc tatggctcgt actctatagg cttcagctgg tgatattgtt
     1741 gagtcaaaac tagagcctgg accactgata tcctgtcttt aacaaattgg actaatcgcc
     1801 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
     1861 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
     1921 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
     1981 tgcatgcacc attccttgcg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     2041 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     2101 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     2161 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     2221 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     2281 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2341 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     2401 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     2461 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2521 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2581 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2641 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2701 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2761 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2821 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2881 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2941 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3001 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3061 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3121 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3181 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3241 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3301 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3361 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3421 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3481 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3541 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3601 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3661 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3721 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3781 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3841 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3901 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3961 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4021 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4081 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4141 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4201 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4261 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4321 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4381 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     4441 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     4501 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     4561 gttgtgtgga attgtgagcg gataacaatt tcacaattct ccccagctct ttctgcgctg
     4621 tccataatga actatttcag gagtattgga gtatgtggat tcaaaacagt ggctaattga
     4681 gtcaggatta cttaatattc gactcctacc ctcagaatgt tgttctccaa cttcaagaaa
     4741 ctgttaccca tgaaagaccc ccgctgacgg gtagtcaatc actcagagga gaccctccca
     4801 aggaacagcg agaccacaag tcggatgcaa ctgcaagagg gtttattgga tacacgggta
     4861 cccgggcgac gcagtctatc ggaggactgg cgcgccgagt gaggggttgt gggctctttt
     4921 attgagctcg gggagcagaa gcgcgcgaac agaagcgaga agcgaactga ttggttagtt
     4981 caaataaggc acagggtcat ttcaggtcct tggggcaccc tggaaacatc tgatggttct
     5041 ctagcttgcc aaacctacag gtggggtctt tcattccccc ctttttctgg agactaaata
     5101 aaatctttta ttttatcgat gcatggggtc gtgcgctcct ttcggtcggg cgctgcgggt
     5161 cgtggggcgg gcgtcaggca ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg
     5221 gcacctcgac gtcggcggtg acggtgaagc cgagccgctc gtagaagggg aggttgcggg
     5281 gcgcggaggt ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc actccgggga
     5341 gcacgacggc gctgcccaga cccttgccct ggtggtcggg cgagacgccg acggtggcca
     5401 ggaaccacgc gggctccttg ggccggtgcg gcgccaggag gccttccatc tgttgctgcg
     5461 cggccagccg ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg aacaccgccc
     5521 ccgcttcgac gctctccggc gtggtccaga ccgccaccgc ggcgccgtcg tccgcgaccc
     5581 acaccttgcc gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc
     5641 gctcgatgtg gcggtccgga tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg
     5701 cggcgagggt gcgtacggcc ctggggacgt cgtcgcgggt ggcgaggcgc accgtgggct
     5761 tgtactcggt catggtaagc ttgggctgca ggtcgaaagg cccggagatg aggaagagga
     5821 gaacagcgcg gcagacgtgc gcttttgaag cgtgcagaat gccgggcctc cggaggacct
     5881 tcgggcgccc gccccgcccc tgagcccgcc cctgagcccg cccccggacc caccccttcc
     5941 cagcctctga gcccagaaag cgaaggagca aagctgctat tggccgctgc cccaaaggcc
     6001 tacccgcttc cattgctcag cggtgctgtc catctgcacg agactagtga gacgtgctac
     6061 ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg ggggggaact tcctgactag
     6121 gggaggagta gaaggtggcg cgaaggggcc accaaagaac ggagccggtt ggcgcctacc
     6181 ggtggatgtg gaatgtgtgc gaggccagag gccacttgtg tagcgccaag tgcccagcgg
     6241 ggctgctaaa gcgcatgctc cagactgcct tgggaaaagc gcctccccta cccggtagaa
     6301 ttcgttatcg aaccactttg tacaagaaag ttggtcaagt gtgctgtccc ctatggccat
     6361 ctcccatccc tgatctcctc ctatctcccc taaatggtcg ccctctgaca tttcgatggt
     6421 actgataacc ttcatcccga cttctgcttg gctgtccttt ccaggactcc tcacttctca
     6481 catgttgata tccaccccta ccagaatagc cccaatcact tttgtacttc ctgcttccat
     6541 aagtttgatt atagaactgc ttggatctac cacttctcaa aatattagac acttcatttt
     6601 catcttctga actctcttct tttggtcttt gcactctgct atcaacagag ttggccccag
     6661 ggctgaccac atcagcagca gtcttaggat cttttacttt ttctgttttt tctttcaggc
     6721 tttgaatggt ccttttaggt tcaagttcaa caggcacagt ttctctctct ctgtttagaa
     6781 tctgagtggg aggatgagcc tttccctctg ctacaggagg gatggaagcc aatgccgtat
     6841 ctgcctttcg tgtctgggaa gattgctctg tccggccttc gtccggctta ctgctcacac
     6901 ttgcttcagg gagagaatgt acatgttcac ccctggcttc tggaaactca tctactgttg
     6961 aaactgaacc acaatcttta gacagatcca gattttccag agacagatcc aattctcctt
     7021 tttcatcaga gggcaggaca atattctgcc tcattactgg attttctatg aatccctgaa
     7081 aagggtaccc ataccaaaca tgaggaaaga aaggaggtgc aataggaact gggcctaaga
     7141 atggattggg tccaaaagat ggctggggga acatattctt gccacttagt gactcttcat
     7201 aatcagcatg cagaagctgc ccaggggtct cagattcaag atcagcctgg taagacaatt
     7261 gtccatgact ttcagacacc tgagatggag ggataaccag aggtgaagaa aatgtcggcg
     7321 gtccaatctc tggctgtggc atttgaccat taacactggc ctcagtctgc ataggaaagt
     7381 gggcatcagt acaggtacaa tcactttcat tctgagcagc aggagcctct tggaaccagg
     7441 gattatgagg ataaacaggg acagggacct ttgggtacat cctgcaggct gccaggtagg
     7501 cctggtgcag agggtacagg taagaatgtg gggcccacat aggacaactg tatgccttca
     7561 caccaagatt gaagaagaat cgaagaatat tcttatcttt aggtaggtcc tccccagtct
     7621 gacacagtga ataaggtggt acaatggctc gatctccctt ttcattacaa ggaaacccag
     7681 gatagagtgg gtcttgataa aggctcaatg tctgaggcaa aggcatcaag ggctggttaa
     7741 ctgcctgtat tgacacagga actggagagg gtgttaaatg agcttgggat acagctgaat
     7801 caggtccagt ggtcaaagtc tgtgtcactg acaaaacggg aattggagca gggacacctg
     7861 ttggtccaaa agttgtaggt tcacttggcc aggctggcac agtggctggt aaagaaggca
     7921 ccgcaggagt tagatgtacc tctggagaaa ctagaagggg agctggattt gacaaagaca
     7981 catgttctgc tggcttttgt tccgcaggag aagggcactc taacttcttt gactttgatg
     8041 gtgatgaaac tgttgcatat ttatcatcag taatattctc caatgtgctt ggttcgggtc
     8101 ttttatccaa ctgactatgt ccatgaatag agtctttgtc ttttcgttct tctgtatcca
     8161 ttctccgcct gcttcctttt ctatcaccta catgtgatga ttttctctgg acacatggat
     8221 tgctactctg agaagctgac tgattgactg atgagctgga aagggctggg aaagcctgtt
     8281 catctctgtt ctgaatctca tagagtaaac gggattcttc tatagcttgc ttctctctgc
     8341 gctcttctgg ggaaaggccg aaatagttag attctcgact tgtgtgatca aaatcctcaa
     8401 ctctttcacg atcaggtttt cttatgatct gtgaaggtgt ccggctaaga tttttgtgct
     8461 cactggagaa tttctgagac tgtgaccctg aagaatgact agagaacgca tgttgtctta
     8521 ctcctgaagg atgctgcagt cgaggaggta gtgctgatgg ggcttttatt ggcttgcttg
     8581 gattctttgg ccctctgtaa tccacatcag aatggaaatt ttgtccagaa gtggaaggtt
     8641 ttttcatctt cttccctgac actgtgttcc agctttctgg gggaggtgcc ttgaggttct
     8701 ttgatgtgtg cttctttccc agttcttcaa ccaaaactgg tccattctca gaatgaattc
     8761 cttgaacatc tgcattcaaa aattttccat tgtgatccaa cctaacttga catttgtctc
     8821 caacttcata ttgtaagcca gcagcaatgg aataatcacg tttttgctga gcttgtttag
     8881 acttcagcca aatttcatat tccacatttc tatagactgc aggattgagt gacttaagaa
     8941 cctttctaga caaaggcagg ctagtagagt tcccattgtt cttcagctgc tcattgcctg
     9001 acaaaggttt aaatccattc acatcagcag cagcagcagc agtcttactc ttgcaactgt
     9061 catcctctga atctgatatt tcactgttat cttcatcagc tacttccaac gtgtctagtt
     9121 ccatcacaat tttactaaca tcagttttaa ataccttctc atacagcaat tcataaagga
     9181 gagactgaca catagcagag ctttctttat actttatggg atacacaata tcataatgat
     9241 ttccatttga aaaacacagt aacacctttt caggaaaatt attttctgtt acttgtgaag
     9301 gagaaacatt tggttcccga taaattataa aatctttcct gtacataaga gaaagggcac
     9361 ttatttccac ttgtcctacc cattcctgtg gattttccaa acgctttaaa tattcttcaa
     9421 atgatccttc tataaacgct tcaaatttct ctctgttctc tcgaagatag tgaatacagg
     9481 ccatgccaac ttttttgtac aaacttgttt gataacctcg agcagcgtaa tctggaacat
     9541 cgtatgggta tcctccatcg agcttgtcat cgtcatcctt gtagtccatg gtggcagatc
     9601 cccggaggct ggatcggtcc cggtgtcttc tatggaggtc aaaacagcgt ggatggcgtc
     9661 tccaggcgat ctgacggttc actaaacgag ctcgtcgacg atctctatca ctgataggga
     9721 gatctctatc actgataggg agagctctgc ttatatagac ctcccaccgt acacgcctac
     9781 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
     9841 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     9901 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
     9961 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
    10021 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
    10081 acacttg
//
