LOCUS       sequence-490486-        5048 bp    DNA     circular SYN 28-OCT-2025
DEFINITION  Express ISCro4 recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Perry NT, Bartie LJ, Katrekar D, Gonzalez GA, Durrant MG, Pai JJ, 
            Fanton A, Martins JQ, Hiraizumi M, Ricci-Tam C, Nishimasu H, 
            Konermann S, Hsu PD
  TITLE     Megabase-scale human genome rearrangement with programmable bridge 
            recombinases.
  JOURNAL   Science. 2025 Sep 25:eadz0276. doi: 10.1126/science.adz0276.
  PUBMED    40997214
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 28, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2025 Sep 25:eadz0276. doi: 10.1126/science.adz0276."
COMMENT     Sequence Label: pNP3614 [pNP3613 - ISCro4 Recombinase (WT)]
FEATURES             Location/Qualifiers
     source          1..5048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(541..559)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        627..731
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(732..800,801..1592)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(950..969)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1763..2351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2252..2271
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2505..2522
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4072..4105
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_RNA        complement(4185..4260)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gacatataca aagcttgttc accatcggaa gcagtaccat cgtataaagc agtatccaaa
       61 ccacacaaag tgaaacccat tcttctataa gcatgaatag ctggagcatt aacattggta
      121 acttccaacc acaaatgacc agcacctctt tctctggcga attcagtagc caaacccatc
      181 aaagctctac caacaccatg acctctatgt tctggagcaa cttcaatatc ttcaacagtc
      241 aatcttctgt tccaaccaga ataagaaaca acaacgaaac cagccaaatc accatcatca
      301 ccataagcaa cgaaagttct agaatctgga tcaccatctt caccagcatc ggattcatca
      361 tcggattcat catctgggaa aaccttagtc aatggtggat caactggaac ttctctcaaa
      421 gtgaaaccat caccagtagc agtaactcta aaaacagtat cggtagtgaa agaaccatcc
      481 aaagcttcaa tagcttcagc atcacctggc ggtttaaacg cgtggccgtg ccgtcaaagg
      541 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      601 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      661 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      721 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      781 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      841 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      901 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      961 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1021 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1081 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1141 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1201 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1261 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1321 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1381 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1441 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1501 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1561 agataggtgc ctcactgatc aagcattggt aactgtcaga ccaagtttac tcatatatac
     1621 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1681 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1741 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1801 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1861 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1921 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1981 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2041 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2101 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2161 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2221 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2281 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2341 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2401 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2461 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2521 aggagtttaa acccagcaaa cgtaacgacg cgaatcagat gagcacactt tcaggatttg
     2581 cctctttggg tgctttcatt tttgctatta ttgtgtaagt aaagggtcaa gaaattctgt
     2641 taagatttga gctattcatt atgtatatca caggttttgc ttaataccat acatcatttc
     2701 ctccttgtta atcaagtgac caatgctgtc gacctcttcc aatagcttgt caacgtcaga
     2761 tatccagttg ttcaacaaat cattgatgtt ctcactatta ctggtaatct gagcagcgcc
     2821 ggcactagta ccatgtttca agttgtcgaa cttgataata ccctggggac ggttgatttt
     2881 cgcggtgatc atacctttgt ttaccatacc actgacatgt gcctccgcat cttgtgaact
     2941 gacttgtaac aaatgagcca accgatctaa cttgataaac agatagtaca tgttgattac
     3001 acgcaaattg tgctcaataa ttctgttctg taagtttgtg ttgttcgtct cgttatcagc
     3061 aaaaacaggt gacttattaa gatagttttc acgatattcc atttcgatat tttcagagtg
     3121 aatgagctca tttgttgtga aaatttccaa aagcttgaac accttagaat ccacattctt
     3181 agaaaaagca gtgttgagct tgattttatg aatcaaatcg gattgaaggt tatcgaatgg
     3241 cgatagaatg atgtagtaga tgatggacac aagcaagtcc ttgtattctg aagattcctt
     3301 gactagtggg atctctatca agagaagtga gttgttgaca accatgatgt agtcattctc
     3361 atgtttgttg atctctagta agtacttcaa gtacgttgcc ttaaatatgt cggccctttc
     3421 aaaatttttc aacgttttta agagaatctt tctgctcaaa atctttgctt gtgcaaagtc
     3481 ttttttttgc aatgtcaacc taatttgctc gagaatgtac tcaattttct cttcaaaagg
     3541 catcaacgag tatgtctcga cctgcaagtc acataagata tctgcggcct tatccaagtc
     3601 attcttcttc tccaagtata tttcagccaa catcttggaa acgaccgccc tctcaacctc
     3661 cacaaagatc ttcttatcag tcaccgtacg aatagtttca ataatcttca tcttcttctc
     3721 taattgcaca ggcttgtcgt tatctaacgt ttcgaggctc ttgatgatct cttgaatcat
     3781 cagttgaatg gagctcttga gttggccatg tttctttgac aaaggcgtga ttaattcatc
     3841 caagtagtcc caatcatcat tatggactag cgtatcaaca attttgctta agatcttctt
     3901 actcagagcc aaatctgaag attgacggat ttgcttttcc aacaccaagt acttctcgat
     3961 agcttgtttg taatcagaag tcttctcaat gagagggatc tgctcttcaa gagtttcaga
     4021 aaaatctttt tcagccttaa taggatcttc tctagacatt ttgatcggcg ggaagttcct
     4081 attctctaga aagtatagga acttcacgcg gtggcggcaa aactaattct tctcttcagt
     4141 tatcattatc cttaaacact cgataaaaaa actcgagaaa aaaagcaccg actcggtgcc
     4201 actttttcaa gttgataacg gactagcctt attttaactt gctatttcta gctctaaaac
     4261 gagacggaat tccgtctcct gttttctgct gagggagtcg aaccctcaca tgccggagcc
     4321 ccgaggctta ccggatgggc cagtgcaaat gaaaatcgta gaactctgcg acttttaaag
     4381 ccatgtcgaa tacgctatac atcaattttt ccgagttttg aggtcaaaat tatccgcatc
     4441 aacacataaa aggtcaaagt ttatgaatgt cgcattcgaa aattgagccc ccgaaaccaa
     4501 ttagtgaact cgtaaaaaat taggaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagaaac
     4561 gtagacgata agagcagcca aaagccatct gtgttcaaaa taactggctc tgagtgttaa
     4621 tcttcactaa ttttacaatg gatgttagat atcagatgcg tgccgcctga tctgagggaa
     4681 gcttacacct ttgtagggta agtgactcta aaagttaagc tgattggggg tttgttttct
     4741 attgcgatat agttttataa actttgataa ggttattgtc tatgaagaag aaaaaaaaaa
     4801 aaaaaaaaaa aaaacttcgt tgattgagtc tgtagcattc acatacgatt gacgcatgat
     4861 attactttct gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg aaaaaaaata
     4921 taaatcacgc taacatttga ttaaaataga acaactacaa tataaaaaaa ctatacaaat
     4981 gacaagttct tgaaaacaag aatcttttta ttgtcagtac tgactcgagt tattatggac
     5041 atggcata
//
