LOCUS       sequence-49050-e         888 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CA6
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             817..837
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             838..855
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             856..879
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtctgact gcacctactc agaaggggca ctggacgaag cgcactggcc acagcactac
       61 cccgcctgtg ggggccagag acagtcgcct atcaacctac agaggacgaa ggtgcggtac
      121 aacccctcct tgaaggggct caatatgaca ggctatgaga cccaggcagg ggagttcccc
      181 atggtcaaca atggccacac agtgcagatc agcctgccct ccaccatgcg catgacagtg
      241 gctgacggca ctgtatacat agcccagcag atgcactttc actggggagg tgcgtcctcg
      301 gagatcagcg gctctgagca caccgtggac gggatcagac atgtgatcga gattcacatt
      361 gttcactaca attctaaata caagagctat gatatagccc aagatgcgcc ggatggtttg
      421 gctgtactgg cagccttcgt tgaggtgaag aattaccctg aaaacactta ttacagcaac
      481 ttcatttctc atctggccaa catcaagtac ccaggacaaa gaacaaccct gactggcctt
      541 gacgttcagg acatgctgcc caggaacctc cagcactact acacctacca tggctcactc
      601 accacgcctc cctgcactga gaacgtccac tggtttgtgc tggcagattt tgtcaagctc
      661 tccaggacac aggtttggaa gctggagaat tccttactgg atcaccgcaa caagaccatc
      721 cacaacgatt accgcaggac ccagcccctg aaccacagag tggtggaatc caacttcccg
      781 aatcaggaat acactctagg ctctgaattc caggcagaga acctctactt ccaatcgcac
      841 catcatcacc accatgatta caaggatgac gacgataagt gaggatcc
//
