LOCUS       sequence-490557-        2811 bp    DNA     circular SYN 24-OCT-2025
DEFINITION  express mGreen Lantern from the cadBA promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Li S, Lee B, Lao Y, Lertwiriyapiti S, Huang X, Wang T
  TITLE     Directed evolution of a plant Rubisco chaperone with altered client 
            recognition.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Sep 16;122(37):e2510701122. doi: 
            10.1073/pnas.2510701122. Epub 2025 Sep 11.
  PUBMED    40932770
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-09-16"; volume: "122(37):e2510701122. 
            doi"; pages: " 10.1073/pnas.2510701122. Epub 2025 Sep 11"
COMMENT     Sequence Label: pSL012
FEATURES             Location/Qualifiers
     source          1..2811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(72..617)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(152..171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(1127..1155)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(1927..2742)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1927..2742)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2650..2669
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2734..2753
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
       61 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
      121 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
      181 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      241 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
      301 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
      361 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
      421 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
      481 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
      541 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagat attacgcgca
      601 gaccaaaacg atctcaagaa gatcatctta ttaaggagcc ccatacgata tacgagagga
      661 catccgggca aatgcaacga aaggctcagt cgaaagactg ggcctttcgt tgcagactta
      721 ggactgtaac tccgggttga tttatgctcg gaaatatttg ttgttgagtt tttgtatgtt
      781 cctgttggta taatatgttg cggcaattta tttgccgcat aatttttatt acataaattt
      841 aaccagagaa tgtcacgcaa tccattgtaa acattaaatg tttatctttt catgatatca
      901 acttgcgatc ctgatgtgtt aataaaaaac ctcaagttct cacttacaga aacttttgtg
      961 ttatttcacc taatctttag gattaatcct tttttcgtga gtaatcttat cgccagtttg
     1021 gtctggtcag gaaatagtta tacatcatga cccggactcc aaattcaaaa atgaaattaa
     1081 ggaggaaaaa aaaatggtga gtaaaggaga agaacttttc actggagttg tcccaattct
     1141 tgttgaatta gatggtgatg ttaatgggca caaattttct gtccgtggag agggtgaagg
     1201 tgatgcaaca aacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
     1261 tccatggcca acacttgtca ctactcttgg ctatggtgtt gcatgctttg ccagataccc
     1321 agatcatatg aaacagcatg actttttcaa gagtgccatg cctgaaggtt atgtacagga
     1381 aagaactata agtttcaaag atgacgggac ctacaagaca cgtgctgaag tcaagtttga
     1441 aggtgatacc cttgttaata gaatcgtgtt aaaaggtatt gattttaaag aagatggaaa
     1501 cattcttgga cacaaattgg aatacaactt taactcacac aaagtataca tcacggcaga
     1561 caaacaaaag aatggaatca aagctaactt caaaactaga cacaacgttg aagatggagg
     1621 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     1681 accagacaac cattacctgt cccatcaatc taaactttcg aaagatccca acgaaaagag
     1741 agaccacatg gtccttaaag agcgtgtaac agctgctggg attacacatg acatggatga
     1801 actatacaaa taaacttaat taacggcact gcgaaaaaac cccgccgaag cggggttttt
     1861 tgcgaaatat aatgaccctc ttgataaccc aagagggcat tttttaatgc ccatggcgta
     1921 cggatcttag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
     1981 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
     2041 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
     2101 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
     2161 gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac
     2221 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
     2281 tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
     2341 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
     2401 aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca
     2461 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
     2521 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     2581 cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg
     2641 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     2701 tcgcggccta gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact
     2761 gtttatgtaa gcagacagtt ttattgttca tgaagagaaa cctacggatc g
//
