LOCUS       sequence-490561-       17350 bp    DNA     circular SYN 24-OCT-2025
DEFINITION  Expresses human ANKRD11 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17350)
  AUTHORS   Sashiyama S, Nakagawa T, Nakagawa M, Hosogane M, Watanabe Y, 
            Ashitomi H, Yamane K, Shibuya N, Moroishi T, Nakayama K, Hosoi T
  TITLE     KBG syndrome-associated protein ANKRD11 regulates SETD5 expression 
            to modulate rRNA levels and translation.
  JOURNAL   iScience. 2025 May 19;28(6):112699. doi: 10.1016/j.isci.2025.112699.
            eCollection 2025 Jun 20.
  PUBMED    40520101
REFERENCE   2  (bases 1 to 17350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2025-05-19"; volume: "28(6):112699. doi"; pages: " 
            10.1016/j.isci.2025.112699. eCollection 2025 Jun 20"
COMMENT     Sequence Label: PB-TA-ERN-ANKRD11
FEATURES             Location/Qualifiers
     source          1..17350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   706..768
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1140..1158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1177..1195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1214..1232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1251..1269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1288..1306
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1325..1343
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1362..1380
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1399..1417
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1439..1463
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1542..1566
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9613..9637)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9639..9662
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(9693..9711)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9694..9711)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9731..9748)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9737..9961
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        10058..10437
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10552..11857
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    10894..11825
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     CDS             join(11874..12491,12492..12497,12498..12533,12534..12536,
                     12537..12572,12573..12575,12576..12611,12612..12620)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    12647..13091
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        13124..13170
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        13162..13180
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             13190..13984
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             13190..13984
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13244..13263)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13854..13873
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    14031..14603
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     repeat_region   14977..15011
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(15160..15178)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(15199..15215)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15199..15215)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15212..15234)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15223..15239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15247..15253,15254..15271,15272..15277))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15292..15313
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15430..15447)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15601..16189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15681..15700)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16360..17151,17152..17220))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16983..17002
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17221..17325)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctcggtattc
      661 acgacagcag gctgaataat aaaaaaatta gaaactatta tttaacccta gaaagataat
      721 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      781 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      841 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      901 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      961 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
     1021 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
     1081 cacatatcaa tattatgctc tcgttaatta actcgaggga tccaagctgg ccgcacgtct
     1141 ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat agagaaggta
     1201 cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc tccctatcag
     1261 tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc gacacgtctc
     1321 gagctcccta tcagtgatag agaaggtacg tctagaacgt ctccctatca gtgatagaga
     1381 agtcgacacg tctcgagctc cctatcagtg atagagaagg taccccctat ataagcagag
     1441 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     1501 gaagacaccg ggaccgatcc agcctccgcg gtggcggcat cacaagtttg tacaaaaaag
     1561 caggctcttt aaaggaacca attcagtcga ctggatccat gcccaagggt gggtgcccta
     1621 aagcaccaca gcaggaagag cttcccctca gcagcgacat ggtggagaag cagactggga
     1681 aaaaggataa agataaagtt tctctaacca agaccccaaa actggagcgt ggcgatggcg
     1741 ggaaggaggt gagggagcga gccagcaagc ggaagctgcc cttcaccgcg ggcgccaatg
     1801 gggagcagaa ggactcggac acagagaagc agggccctga gcggaagagg attaagaagg
     1861 agcctgtcac ccggaaggcc gggctgctgt ttggcatggg gctgtctgga atccgagccg
     1921 gctaccccct ctccgagcgc cagcaggtgg cccttctcat gcagatgacg gccgaggagt
     1981 ctgccaacag cccagtggac acaacaccaa agcacccctc ccagtctaca gtgtgtcaga
     2041 agggaacgcc caactctgcc tcaaaaacca aagataaagt gaacaagaga aacgagcgtg
     2101 gagagacccg cctgcaccga gccgccatcc gcggggacgc ccggcgcatc aaagagctca
     2161 tcagcgaggg ggcagacgtc aacgtcaagg acttcgcagg ctggacggcg ctgcacgagg
     2221 cctgtaaccg gggctactac gacgtcgcga agcagctgct ggctgcaggt gcggaggtga
     2281 acaccaaggg cctagatgac gacacgcctt tgcacgacgc tgccaacaac gggcactaca
     2341 aggtggtgaa gctgctgctg cggtacggag ggaacccgca gcagagcaac aggaaaggcg
     2401 agacgccgct gaaagtggcc aactccccca cgatggtgaa cctcctgtta ggcaaaggca
     2461 cttacacttc cagcgaggag agctcgacgg agagctcaga agaggaagac gcaccatcct
     2521 tcgcaccttc cagttcagtc gacggcaaca acacggactc cgagttcgaa aaaggcctca
     2581 agcacaaggc caagaaccca gagccacaga aggccacggc ccccgtcaag gacgagtatg
     2641 agtttgatga ggacgacgag caggacaggg ttcctccggt ggacgacaag cacctattga
     2701 aaaaggacta cagaaaagaa acgaaatcca atagttttat ctctataccc aaaatggagg
     2761 ttaaaagtta cactaaaaat aacacgattg caccaaagaa agcgtcccat cgtatcctgt
     2821 cagacacgtc ggacgaggag gacgcgagtg tcaccgtggg gacaggagag aagctgagac
     2881 tctcggcaca tacgatattg cctggtagta agacacgaga gccttctaat gccaagcagc
     2941 agaaggaaaa aaataaagtg aaaaagaagc gaaagaaaga aacaaaaggc agagaggttc
     3001 gcttcggaaa gcggagcgac aagttctgct cctcggagtc ggagagcgag tcctcagaga
     3061 gtggggagga tgacagggac tctctgggga gctctggctg cctcaagggg tccccgctgg
     3121 tgctgaagga cccctccctg ttcagctccc tctctgcctc ctccacctcg tctcacggga
     3181 gctctgccgc ccagaagcag aaccccagcc acacagacca gcacaccaag cactggcgga
     3241 cagacaattg gaaaaccatt tcttccccgg cttggtcaga ggtcagttct ttatcagact
     3301 ccacaaggac gagactgaca agcgagtctg actactcctc tgagggctcc agtgtggaat
     3361 cgctgaagcc agtgaggaag aggcaggagc acaggaagcg agcctccctg tcggagaaga
     3421 agagcccctt cctgtccagc gcggagggcg ctgtccccaa actggacaag gaggggaaag
     3481 ttgtcaaaaa acataaaaca aaacacaaac acaaaaacaa ggagaaggga cagtgttcca
     3541 tcagccaaga gctgaagttg aaaagtttta cttacgaata tgaggactcc aagcagaagt
     3601 cagataaggc tatactgtta gagaatgatc tttccactga aaacaagcta aaagtgttaa
     3661 agcacgatcg cgaccacttt aaaaaagaag agaaacttag caaaatgaaa ttagaagaaa
     3721 aagaatggct ctttaaagat gaaaaatcac tgaagagaat caaagacaca aacaaagaca
     3781 tcagcaggtc tttccgagaa gagaaagacc gttcgaataa agcagaaaag gagagatcgc
     3841 tgaaggaaaa gtctccgaaa gaagaaaaac tgagactgta caaagaggag agaaagaaga
     3901 aatcaaaaga ccggccctca aaattagaga agaagaatga tttaaaagag gacaaaattt
     3961 caaaagagaa ggagaagatt tttaaagaag ataaagaaaa actcaaaaaa gaaaaggttt
     4021 atagggaaga ttctgctttt gacgaatatt gtaacaaaaa tcagtttctg gagaatgaag
     4081 acaccaaatt tagcctttct gacgatcagc gagatcggtg gttttctgac ttgtccgatt
     4141 catcctttga tttcaaaggg gaggacagct gggactcgcc agtgacagac tacagggaca
     4201 tgaagagcga ctctgtggcc aagctcatct tggagacggt gaaggaggac agcaaggaga
     4261 ggaggcggga cagccgggcc cgggagaagc gagactacag agagcccttc ttccgaaaga
     4321 aggacaggga ctatttggat aaaaactctg agaagaggaa agagcagact gaaaagcata
     4381 aaagtgtccc tggctacctt tcggaaaagg acaagaagag gagagagtcc gcagaggccg
     4441 ggcgggacag aaaggacgcc ctggagagct gcaaggagcg cagggacggc agggccaagc
     4501 ccgaggaggt gcaccgggag gagctgaagg agtgtggctg cgagagtggc ttcaaggaca
     4561 agtccgacgg cgactttggg aagggcctgg agccgtggga acggcaccac ccagcacgag
     4621 agaaggagaa gaaggatggc cccgataagg aaaggaagga gaagacaaaa ccagaaagat
     4681 acaaagagaa atccagtgac aaggacaaaa gtgagaaatc aatcctggaa aaatgtcaga
     4741 aggacaaaga atttgataaa tgttttaaag agaaaaaaga taccaaggaa aaacataaag
     4801 acacacatgg caaagacaaa gaaaggaaag cgtctctcga ccaagggaaa gagaagaagg
     4861 agaaggcttt ccctgggatc atctcagaag acttctctga aaaaaaagat gacaagaaag
     4921 gcaaagagaa aagctggtac atcgcagaca tcttcacaga tgagagtgag gacgacagag
     4981 acagctgcat ggggagcggg ttcaagatgg gagaggccag cgacttgccg aggacggacg
     5041 gcctccagga gaaggaggaa ggacgggagg cctatgcctc cgacagacac aggaagtctt
     5101 ctgacaagca gcaccctgag aggcagaagg acaaggagcc cagagacagg agaaaggacc
     5161 gaggggctgc cgacgcgggg agagacaaaa aagagaaagt ctttgaaaag cacaaggaga
     5221 agaaggataa agagtccaca gaaaagtaca aggacaggaa ggacagagcc tcagtggact
     5281 ccacgcaaga taagaaaaat aaacagaagc tccccgagaa ggctgaaaag aagcacgctg
     5341 ccgaagacaa ggctaaaagc aaacacaaag agaagtcgga caaagaacat tccaaggaga
     5401 ggaagtcctc gagaagtgcc gacgcggaaa aaagcctgct tgaaaagttg gaagaagagg
     5461 ctctccatga gtacagagaa gactccaacg ataaaatcag cgaggtctcc tctgacagct
     5521 tcacggaccg agggcaggag ccggggctga ctgccttcct ggaggtctct ttcacggagc
     5581 cacctggaga cgacaagccg agggagagcg cctgcctccc tgagaagctg aaagagaagg
     5641 agaggcacag acactcctca tcttcatcca agaagagcca cgaccgagag cgagccaaga
     5701 aagagaaggc cgagaagaaa gagaagggcg aagattacaa ggagggcggt agcaggaagg
     5761 actccggcca gtacgaaaag gacttcctgg aggcggatgc ttacggagtt tcttacaaca
     5821 tgaaagctga catagaagat gagctagata aaaccattga attgttttct accgaaaaga
     5881 aagataaaaa tgattccgag agagaacctt ccaagaaaat agaaaaggaa ctaaagcctt
     5941 atggatctag tgccatcaac atcctaaaag agaagaagaa gagagagaaa cacagggaga
     6001 aatggagaga cgagaaggag aggcaccggg acaggcatgc ggatgggctg ctgcggcatc
     6061 acagggacga gctcctgcgg catcacaggg acgagcagaa gcccgccacc agggacaagg
     6121 acagcccgcc ccgcgtgctc aaagacaagt ccagggacga gggcccgagg ctcggcgatg
     6181 ccaaactgaa ggagaaattc aaggacggtg cagagaaaga aaagggcgac ccagtgaaga
     6241 tgagcaacgg gaatgataag gtagcgccat ccaaagaccc aggcaagaaa gacgccaggc
     6301 ccagggagaa gctcctgggg gacggcgacc tgatgatgac cagcttcgag aggatgctgt
     6361 cccagaagga cctggagatc gaggagcgcc acaagcggca caaggagagg atgaagcaaa
     6421 tggagaagct gaggcaccgg tccggagacc ccaagctcaa ggagaaggcg aagccggcag
     6481 acgacgggcg gaagaagggt ctggacattc ctgctaagaa accgccgggg ctggaccctc
     6541 catttaaaga caaaaagctc aaagagtcga ctcctattcc acctgccgcg gaaaataagc
     6601 tacacccagc atcaggtgca gactccaaag actggctggc aggccctcac atgaaagagg
     6661 tcctgcctgc gtcccccagg cctgaccaga gccggcccac tggcgtgccc acccctacgt
     6721 cggtgctatc ctgccccagc tacgaggagg tgatgcacac gcccaggacc ccgtcctgca
     6781 gcgccgatga ctacgcggac ctcgtgttcg actgcgccga ctcgcagcac tccacgcccg
     6841 tgcccaccgc tcccaccagc gcctgctccc cctccttttt cgacaggttc tccgtggctt
     6901 caagtgggct ttcggaaaac gccagccagg ctcctgccag gcctctctcc acaaaccttt
     6961 accgctcggt ctctgtcgac attaggagga cccccgagga agaattcagc gtcggagaca
     7021 agctcttcag gcagcagagc gttcctgctg cctccagcta cgactctccc atgccaccct
     7081 cgatggaaga cagggcgccc ctgcccccgg ttcccgcgga gaagtttgcc tgcttgtcgc
     7141 cagggtacta ctccccagac tatggcctcc cgtcgcccaa agtcgacgct ttgcactgcc
     7201 caccggctgc cgttgtcact gtcaccccgt ctccagaggg cgtcttctca agtttacaag
     7261 caaaaccttc cccttccccc agagccgagc tgctggttcc ttccctcgaa ggggcccttc
     7321 ccccggacct ggacacctcc gaggaccagc aggcgacggc cgccatcatc cccccggagc
     7381 ccagctacct ggagccgctg gacgagggtc ccttcagcgc cgtcatcacc gaggagcccg
     7441 ttgagtgggc ccacccctcc gagcaggcgc ttgcctctag cctgatcggg ggcacctctg
     7501 aaaaccctgt gagctggcct gtgggctcgg acctcctgct gaagtctcca cagagattcc
     7561 ccgagtcccc aaagcgtttc tgccccgcgg accccctcca ctctgccgcc ccagggccct
     7621 tcagcgcctc ggaggcgccg taccccgccc ctcccgcctc tcctgccccg tacgctctgc
     7681 ccgtcgctga gccggggctg gaggacgtca aggacggagt ggacgccgtc cccgccgcca
     7741 tctccacctc agaggcggct ccctacgccc ctccctccgg gctggagtcc ttcttcagca
     7801 actgcaagtc acttccggaa gccccgctgg acgtggcccc cgagcccgcc tgtgtagccg
     7861 ctgtggctca ggtggaggct ctggggcccc tggaaaatag cttcctggac ggcagccgcg
     7921 gcctgtctca cctcggccag gtggagccgg tgccctgggc ggacgccttc gccggccccg
     7981 aggacgacct ggacctgggg cccttctccc tgccggagct tcccctgcag actaaagatg
     8041 ccgcagatgg tgaagcggaa cccgtggaag aaagtcttgc tcctccagaa gagatgcctc
     8101 caggggcccc cggggtcata aacggtgggg atgtttccac cgtagtggct gaggagccgc
     8161 cggcactgcc tcctgaccag gcctccaccc ggctccctgc agagctcgag cctgagccct
     8221 caggggagcc aaagctggac gtggctctag aagctgcggt ggaggcggag acggtgccgg
     8281 aagagagggc ccgtggggat ccggactcca gcgtggagcc cgcgcccgtt cccccagaac
     8341 agcgcccact ggggagcgga gaccaggggg ctgaggctga aggccccccc gccgcgtccc
     8401 tctgtgcccc tgacggcccc gccccgaaca ctgtggcaca agctcaggcc gcagacggtg
     8461 ccggccccga ggacgacact gaggcctccc gtgccgccgc cccagccgaa ggccctcctg
     8521 gcggcatcca gccggaagcc gcagaaccaa aacccacggc cgaagccccg aaggcccccc
     8581 gagtggagga gatccctcag cgcatgacca ggaaccgggc gcagatgctc gcgaaccaga
     8641 gcaagcaggg cccgcccccc tccgagaagg agtgcgcccc cacccctgcc ccggtcacca
     8701 gggccaaggc ccgcggctcc gaggacgacg acgcccaggc ccagcatccg cgcaaacgcc
     8761 gctttcagcg ctccacccag cagctgcagc agcagctgaa cacgtccacg cagcagacgc
     8821 gggaggtgat ccagcagacg ctggccgcca tcgtggacgc catcaagctg gatgccatcg
     8881 agccctacca cagcgacagg gccaacccct acttcgaata cctgcagatc aggaagaaga
     8941 tcgaggagaa gcgcaagatc ctgtgctgta tcacgccgca ggcgccccag tgctacgccg
     9001 agtacgtcac ctacacgggc tcctacctcc tggacggcaa gccgctcagc aagctccaca
     9061 tccccgtgat cgcaccccct ccctccctgg cggagcccct gaaggagctg ttcaggcagc
     9121 aggaggccgt ccggggaaag ctgcgtctac agcacagcat cgagcgggag aagctgatcg
     9181 tatcctgtga gcaggagatt ctgcgggttc actgccgggc ggccaggacc atcgccaacc
     9241 aggcagtgcc attcagcgcc tgcacgatgc tgctggactc cgaggtctac aacatgcccc
     9301 tggagagcca gggtgacgag aacaagtcag tgcgcgaccg tttcaacgcc cgccagttca
     9361 tctcctggct ccaggacgtg gatgacaagt atgaccgcat gaagacttgc ctcctcatgc
     9421 ggcagcagca cgaggccgcg gccctgaacg ccgtgcagag gatggagtgg cagctgaagg
     9481 tgcaggaact ggaccccgcc gggcacaagt ccctgtgcgt gaacgaggtg ccctccttct
     9541 acgtgcccat ggtcgacgtc aacgacgact ttgtattgtt gccggcatgg cggccgcact
     9601 cgagatatct agacccagct ttcttgtaca aagtggttga ctacaaagac gatgacgaca
     9661 agtgactcga gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct
     9721 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     9781 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     9841 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     9901 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     9961 gcttctgagg cggaaagaac cagatctgta cattcgaatc gatgaattcg ttaaccatgg
    10021 aagcttctgc agcggccgca ctagcggtgg cggcctcgac attgattatt gactagttat
    10081 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
    10141 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
    10201 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
    10261 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
    10321 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
    10381 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgatg
    10441 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
    10501 agtctccacc ccattgacgt caatgggagt ttgttttgac tagtggagcc gagagtaatt
    10561 catacaaaag gagggatcgc cttcgcaagg ggagagccca gggaccgtcc ctaaattctc
    10621 acagacccaa atccctgtag ccgccccacg acagcgcgag gagcatgcgc ccagggctga
    10681 gcgcgggtag atcagagcac acaagctcac agtccccggc ggtgggggga ggggcgcgct
    10741 gagcgggggc cagggagctg gcgcggggca aactgggaaa gtggtgtcgt gtgctggctc
    10801 cgccctcttc ccgagggtgg gggagaacgg tatataagtg cggtagtcgc cttggacgtt
    10861 ctttttcgca acgggtttgc cgtcagaacg caggtgagtg gcgggtgtgg cttccgcggg
    10921 ccccggagct ggagccctgc tctgagcggg ccgggctgat atgcgagtgt cgtccgcagg
    10981 gtttagctgt gagcattccc acttcgagtg gcgggcggtg cgggggtgag agtgcgaggc
    11041 ctagcggcaa ccccgtagcc tcgcctcgtg tccggcttga ggcctagcgt ggtgtccgcc
    11101 gccgcgtgcc actccggccg cactatgcgt tttttgtcct tgctgccctc gattgccttc
    11161 cagcagcatg ggctaacaaa gggagggtgt ggggctcact cttaaggagc ccatgaagct
    11221 tacgttggat aggaatggaa gggcaggagg ggcgactggg gcccgcccgc cttcggagca
    11281 catgtccgac gccacctgga tggggcgagg cctgtggctt tccgaagcaa tcgggcgtga
    11341 gtttagccta cctgggccat gtggccctag cactgggcac ggtctggcct ggcggtgccg
    11401 cgttcccttg cctcccaaca agggtgaggc cgtcccgccc ggcaccagtt gcttgcgcgg
    11461 aaagatggcc gctcccgggg ccctgttgca aggagctcaa aatggaggac gcggcagccc
    11521 ggtggagcgg gcgggtgagt cacccacaca aaggaagagg gccttgcccc tcgccggccg
    11581 ctgcttcctg tgaccccgtg gtctatcggc cgcatagtca cctcgggctt ctcttgagca
    11641 ccgctcgtcg cggcgggggg aggggatcta atggcgttgg agtttgttca catttggtgg
    11701 gtggagacta gtcaggccag cctggcgctg gaagtcattc ttggaatttg cccctttgag
    11761 tttggagcga ggctaattct caagcctctt agcggttcaa aggtattttc taaacccgtt
    11821 tccaggtgtt gtgaaagcca ccgctaattc aaagcaatcc ggagtaattc accatgtcta
    11881 gactggacaa gagcaaagtc ataaacggcg ctctggaatt actcaatgga gtcggtatcg
    11941 aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact
    12001 ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc
    12061 atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg
    12121 ccaagtcatt ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca
    12181 cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc
    12241 aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg
    12301 gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca
    12361 ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg
    12421 aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt
    12481 gcgaaagcgg cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg
    12541 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
    12601 ttgacatgct ccccgggtaa ctaagtaagg atcccgtacg cctaggagca ggtttcccca
    12661 atgacacaaa acgtgcaact tgaaactccg cctggtcttt ccaggtctag aggggtaaca
    12721 ctttgtactg cgtttggctc cacgctcgat ccactggcga gtgttagtaa cagcactgtt
    12781 gcttcgtagc ggagcatgac ggccgtggga actcctcctt ggtaacaagg acccacgggg
    12841 ccaaaagcca cgcccacacg ggcccgtcat gtgtgcaacc ccagcacggc gactttactg
    12901 cgaaacccac tttaaagtga cattgaaact ggtacccaca cactggtgac aggctaagga
    12961 tgcccttcag gtaccccgag gtaacacgcg acactcggga tctgagaagg ggactggggc
    13021 ttctataaaa gcgctcggtt taaaaagctt ctatgcctga ataggtgacc ggaggtcggc
    13081 acctttcctt tgcaattact gaccctatga atacactgac tgtttgacaa ttaatcatcg
    13141 gcatagtata tcggcatagt ataatacgac tcactatagg agggccacca tgattgaaca
    13201 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
    13261 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
    13321 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
    13381 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
    13441 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
    13501 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
    13561 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
    13621 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
    13681 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
    13741 cgtcgtgaca catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
    13801 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
    13861 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
    13921 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
    13981 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga attcgctagg attatcccta
    14041 atacctgcca ccccactctt aatcagtggt ggaagaacgg tctcagaact gtttgtttca
    14101 attggccatt taagtttagt agtaaaagac tggttaatga taacaatgca tcgtaaaacc
    14161 ttcagaagga aaggagaatg ttttgtggac cactttggtt ttcttttttg cgtgtggcag
    14221 ttttaagtta ttagttttta aaatcagtac tttttaatgg aaacaacttg accaaaaatt
    14281 tgtcacagaa ttttgagacc cattaaaaaa gttaaatgag aaacctgtgt gttcctttgg
    14341 tcaacaccga gacatttagg tgaaagacat ctaattctgg ttttacgaat ctggaaactt
    14401 cttgaaaatg taattcttga gttaacactt ctgggtggag aatagggttg ttttcccccc
    14461 acataattgg aaggggaagg aatatcattt aaagctatgg gagggttgct ttgattacaa
    14521 cactggagag aaatgcagca tgttgctgat tgcctgtcac taaaacaggc caaaaactga
    14581 gtccttgggt tgcatagaaa gctgccaaac gctagccaac aagctcgtca tcgctttgca
    14641 gaagagcaga gaggatatgc tcatcgtcta aagaactacc cattttatta tatattagtc
    14701 acgatatcta taacaagaaa atatatatat aataagttat cacgtaagta gaacatgaaa
    14761 taacaatata attatcgtat gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt
    14821 cattttgact cacgcggtcg ttatagttca aaatcagtga cacttaccgc attgacaagc
    14881 acgcctcacg ggagctccaa gcggcgactg agatgtccta aatgcacagc gacggattcg
    14941 cgctatttag aaagagagag caatatttca agaatgcatg cgtcaatttt acgcagacta
    15001 tctttctagg gttaaaaaag atttgcgctt tactcgacct aaactttaaa cacgtcatag
    15061 aatcttcgtt tgacaaaaac cacattgtgg ccaagctgtg tgacgcgacg cgcgctaaag
    15121 aatggcaaac caagtcgcgc gaggtaccag cttttgttcc ctttagtgag ggttaatttc
    15181 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    15241 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    15301 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    15361 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    15421 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    15481 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    15541 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    15601 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    15661 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    15721 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    15781 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    15841 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    15901 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    15961 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    16021 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    16081 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    16141 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    16201 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    16261 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    16321 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    16381 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    16441 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    16501 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    16561 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    16621 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    16681 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    16741 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    16801 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    16861 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    16921 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    16981 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    17041 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    17101 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    17161 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    17221 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    17281 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    17341 agtgccacct
//
