LOCUS       sequence-490566-       10853 bp    DNA     circular SYN 24-OCT-2025
DEFINITION  Used for multi-site piggybac transposon insertion of CRY2-tagged 
            G3BP2 for overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10853)
  TITLE     Bartel lab plasmids 2025
REFERENCE   2  (bases 1 to 10853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DBJS-p9.6
FEATURES             Location/Qualifiers
     source          1..10853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        324..702
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    332..350
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    368..386
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    404..422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    440..458
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    476..494
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    512..530
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    548..566
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     611..635
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3235..3276
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             join(3283..3285,3286..3996)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3283..3285,3286..3996)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3325..3346)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3586..3605)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3933..3954
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4083..4102)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4127..4175
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        4406..4617
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4630..4898
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4932..5675
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             5676..5729
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5730..6329
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5730..5749)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6226..6246
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6362..6483
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6399..6418)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6453..6472
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7246..7280
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7771..7787)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7771..7787)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7784..7806)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7795..7811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7819..7825,7826..7843,7844..7849))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7864..7885
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8002..8019)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8173..8761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8253..8272)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8932..9723,9724..9792))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9555..9574
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9793..9897)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9923..10378)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10060..10081)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10272..10291
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10505..10527
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10519..10536
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10520..10536
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   10666..10728
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca aaacttttat gagggacagc
       61 ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga
      121 gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc
      181 ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc
      241 tgctctttga gcctgcagac acctgggggg atacggggaa aaggcctcca cggccactag
      301 tctttcgtct tcaagaattc ctcgagttta ctccctatca gtgatagaga acgtatgaag
      361 agtttactcc ctatcagtga tagagaacgt atgcagactt tactccctat cagtgataga
      421 gaacgtataa ggagtttact ccctatcagt gatagagaac gtatgaccag tttactccct
      481 atcagtgata gagaacgtat ctacagttta ctccctatca gtgatagaga acgtatatcc
      541 agtttactcc ctatcagtga tagagaacgt ataagcttta ggcgtgtacg gtgggcgcct
      601 ataaaagcag agctcgttta gtgaaccgtc agatcgcctg gagcaattcc acaacacttt
      661 tgtcttatac caactttccg taccacttcc taccctcgta aaggtctaga gctagccacc
      721 atgatgaaga tggacaaaaa gaccatcgtc tggtttcgga gagatttgag aatagaagat
      781 aatcccgcgc tcgccgccgc ggcccacgag ggttccgtct tccccgtttt catttggtgt
      841 cctgaagaag aaggccagtt ttatcccgga agggcctcta ggtggtggat gaagcaaagt
      901 ctggcccatc ttagccagtc actgaaagca ctgggcagtg atcttaccct gatcaagaca
      961 cacaatacca tctctgccat cctcgactgc atcagggtga ccggcgcaac gaaagtcgtg
     1021 tttaaccacc tgtacgatcc agttagtctg gtgcgcgacc acactgtgaa ggagaagctg
     1081 gtggaacggg ggatcagtgt gcagagctac aacggggacc ttctgtacga gccatgggag
     1141 atctattgcg agaaagggaa accgttcacc tccttcaaca gttactggaa gaaatgtttg
     1201 gatatgtcaa tagagtccgt tatgttgccc cctccctgga gactgatgcc gattactgct
     1261 gctgcagagg ccatctgggc ctgctccatc gaggaactcg gtctggaaaa tgaagcagaa
     1321 aagccaagca atgcacttct cactagagcc tggagccccg gctggtctaa tgccgacaag
     1381 ctgcttaacg agttcatcga aaaacaactg attgactacg cgaagaactc caagaaagtg
     1441 gtaggtaact caactagctt gctctctcca tatctccatt ttggcgagat ttctgtccgc
     1501 catgtatttc agtgcgctcg gatgaaacag attatctggg ctcgcgataa aaacagcgaa
     1561 ggcgaagaaa gcgccgatct gttcctgcga gggatcggac ttcgggaata ctcccggtat
     1621 atatgtttca actttccatt cacacacgag cagagtctgt tgtcccacct caggttcttc
     1681 ccctgggacg ccgatgtcga caaattcaag gcatggagac agggaaggac agggtaccca
     1741 ctcgtggatg ctggcatgag agagctctgg gctacaggct ggatgcacaa ccgcatccgg
     1801 gtaatcgtgt cctcatttgc tgtcaagttt ctgctcctgc cttggaaatg gggaatgaag
     1861 tacttttggg atacccttct cgacgccgac ttggagtgtg acattctggg atggcaatat
     1921 attagcgggt caattcctga cggccatgag ttggacaggt tggacaatcc ggccttgcag
     1981 ggagctaagt atgatcccga aggagagtat attcgacagt ggctccccga gctggcccga
     2041 cttcctacgg agtggattca ccatccttgg gacgcaccac tgacagtgct caaggcaagc
     2101 ggggtggagc tgggcaccaa ttacgctaag cctatagttg atatagatac agcacgcgag
     2161 ctgctggcta aagcgatctc tcgcactcgg gaggcgcaga ttatgatcgg tgctgccgaa
     2221 cttgatgaag aatcagaaga tgaagtagaa gaggaacaag aagaaagaca accatctcct
     2281 gaacctgtgc aagaaaatgc taacagtggt tactatgaag ctcaccctgt gactaatggc
     2341 atagaggagc ctttggaaga atcctctcat gaacctgaac ctgagccaga atctgaaaca
     2401 aagactgaag agctgaaacc acaagtggag gagaagaact tagaagaact agaggagaaa
     2461 tctactactc ctcctccggc agaacctgtt tctctgccac aagaaccacc aaaggctttc
     2521 tcctgggctt cagtgaccag taaaaacctg cctcctagtg gtactgtttc ttcctctgga
     2581 attccacccc atgttaaagc accagtctca cagccaagag tcgaagctaa accagaagtt
     2641 caatctcagc cacctcgtgt gcgtgaacaa cgacctagag aacgacctgg ttttcctcct
     2701 agaggaccaa gaccaggcag aggagatatg gaacagaatg actctgacaa ccgtagaata
     2761 attcgctatc cagatagtca tcaacttttt gttggtaact tgccacatga tattgatgaa
     2821 aatgagctaa aggaattctt catgagtttt ggaaacgttg tggaacttcg catcaatacc
     2881 aagggtgttg ggggaaagct tccaaatttt ggttttgtgg tttttgatga ctctgaacca
     2941 gttcagagaa tcttaattgc aaaaccgatt atgtttcgag gggaagtacg tttaaatgtg
     3001 gaagagaaaa aaacaagagc tgcaagagag cgagaaacca gaggtggtgg tgatgatcgc
     3061 agggatatta ggcgcaatga tcgaggtccc ggtggtccac gtggaattgt gggtggtgga
     3121 atgatgcgtg atcgtgatgg aagaggacct cctccaaggg gtggcatggc acagaaactt
     3181 ggctctggaa gaggaaccgg gcaaatggag ggccgcttca caggacagcg tcgctccgga
     3241 ttcgccaacg agctcggacc acgtctcatg ggaaagggta ccgtgagcaa gggcgaggag
     3301 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     3361 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     3421 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     3481 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     3541 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     3601 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     3661 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     3721 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     3781 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     3841 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     3901 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     3961 gccgggatca ctctcggcat ggacgagctg tacaagaccg gttgaccggt tagtaatgag
     4021 tttaaacggg ggaggaagac cggttgaccg gttagtaatg agtttaaacg ggggaggcta
     4081 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     4141 cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     4201 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     4261 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     4321 ggcggcaggc cctgccatag cagatctgcg aaatcggatc cagctgaatc taagtggatc
     4381 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     4441 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     4501 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     4561 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     4621 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     4681 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     4741 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     4801 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     4861 gtctttgttt cgttttctgt tctgcgccgt tacagatcta agctgtgacc ggcgcctact
     4921 ctagagccgc catgtctaga ctggacaaga gcaaagtcat aaactctgct ctggaattac
     4981 tcaatggagt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg
     5041 agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa
     5101 tcgagatgct ggacaggcat catacccact cctgccccct ggaaggcgag tcatggcaag
     5161 actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat cgcgacgggg
     5221 ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc
     5281 tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg
     5341 tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg
     5401 aaagagagac acctaccacc gattctatgc ccccacttct gaaacaagca attgagctgt
     5461 tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc
     5521 tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac gattttgact
     5581 tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg
     5641 ctcttgacga ttttgacctt gacatgctcc ccggggaggg cagaggaagt cttctaacat
     5701 gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg gtgcgcctcg
     5761 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     5821 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     5881 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     5941 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     6001 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     6061 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     6121 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     6181 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     6241 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     6301 ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa atttgtgggt
     6361 aaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6421 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6481 ttatcatgtc tggaattgac tcaaatgatg tcaattagtc tatcagaagc tcatctggtc
     6541 tcccttccgg gggacaagac atccctgttt aatatttaaa cagcagtgtt cccaaactgg
     6601 gttcttatat cccttgctct ggtcaaccag gttgcagggt ttcctgtcct cacaggaacg
     6661 aagtccctaa agaaacagtg gcagccaggt ttagccccgg aattgactgg attccttttt
     6721 tagggcccat tggtatggct ttttccccgt atccccccag gtgtctgcag gctcaaagag
     6781 cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc
     6841 ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg
     6901 gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg
     6961 gggctgtccc tgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag
     7021 aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata
     7081 atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca
     7141 ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg
     7201 acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta
     7261 cgcagactat ctttctaggg ttaatctagc tgcatcagga tcatatcgtc gggtcttttt
     7321 tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg
     7381 ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca
     7441 ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg
     7501 cctttaacgg tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc
     7561 ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca
     7621 gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg
     7681 tcagcgagga cgggtatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt
     7741 tacccggcgg gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7801 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7861 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7921 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7981 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8041 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8101 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8161 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8221 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8281 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8341 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8401 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8461 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8521 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8581 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8641 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8701 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8761 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8821 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8881 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8941 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9001 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9061 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9121 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9181 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9241 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9301 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9361 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9421 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9481 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9541 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9601 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9661 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9721 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9781 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9841 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9901 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     9961 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
    10021 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
    10081 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
    10141 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
    10201 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
    10261 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
    10321 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
    10381 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
    10441 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
    10501 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt
    10561 tttgaacccg tggaggacgg gcagactcgc ggtgcaaatg tgttttacag cgtgatggag
    10621 cagatgaaga tgctcgacac gctgcagaac acgcagctag attaacccta gaaagataat
    10681 catattgtga cgtacgttaa agataatcat gtgtaaaatt gacgcatgtg ttttatcggt
    10741 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
    10801 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt att
//
