LOCUS       sequence-490568-       11182 bp    DNA     circular SYN 24-OCT-2025
DEFINITION  Used for multi-site piggybac transposon insertion of CRY2-tagged 
            G3BP1 for overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11182)
  TITLE     Bartel lab plasmids 2025
REFERENCE   2  (bases 1 to 11182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DBJS-p8.15
FEATURES             Location/Qualifiers
     source          1..11182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    948..1069
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(985..1004)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1039..1058
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   1832..1866
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2357..2373)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2357..2373)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2370..2392)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2381..2397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2405..2411,2412..2429,2430..2435))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2450..2471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2588..2605)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2759..3347)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2839..2858)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3518..4309,4310..4378))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4141..4160
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4379..4483)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4509..4964)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4646..4667)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4858..4877
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5091..5113
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5105..5122
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5106..5122
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   5252..5314
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        5763..6141
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    5771..5789
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5807..5825
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5843..5861
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5879..5897
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5915..5933
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5951..5969
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5987..6005
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6050..6074
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(6157..6159,6160..6162,6163..6873)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6157..6159,6160..6162,6163..6873)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6202..6223)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6463..6482)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6810..6831
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9425..9444)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9469..9517
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        9748..9959
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             9972..10240
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             10274..11017
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             11018..11071
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(11078..11182,1..921)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             join(11078..11182,1..921)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
       61 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
      121 gaagtgcttg acattgggga atttagcgag agcctgacct attgcatctc ccgccgtgca
      181 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
      241 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
      301 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
      361 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
      421 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
      481 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
      541 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
      601 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
      661 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
      721 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
      781 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
      841 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
      901 cgtccgaggg caaaggaata gcctctggat tacaaaattt gtgggttaac ttgtttattg
      961 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1021 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     1081 attgactcaa atgatgtcaa ttagtctatc agaagctcat ctggtctccc ttccggggga
     1141 caagacatcc ctgtttaata tttaaacagc agtgttccca aactgggttc ttatatccct
     1201 tgctctggtc aaccaggttg cagggtttcc tgtcctcaca ggaacgaagt ccctaaagaa
     1261 acagtggcag ccaggtttag ccccggaatt gactggattc cttttttagg gcccattggt
     1321 atggcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt
     1381 cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg
     1441 ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc gctgctgccc
     1501 cctagcgggg gagggacgta attacatccc tgggggcttt gggggggggc tgtccctgat
     1561 atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca
     1621 atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt
     1681 tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc
     1741 tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta
     1801 tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt
     1861 ctagggttaa tctagctgca tcaggatcat atcgtcgggt cttttttccg gctcagtcat
     1921 cgcccaagct ggcgctatct gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag
     1981 gttgaagcgg catggaaaga gtttgccgag gatgactgct gctgcattga cgttgagcga
     2041 aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa
     2101 ctgttcgttc aggccacctg ggataccagt tcgtcgcggc ttttccggac acagttccgg
     2161 atggtcagcc cgaagcgcat cagcaacccg aacaataccg gcgacagccg gaactgccgt
     2221 gccggtgtgc agattaatga cagcggtgcg gcgctgggat attacgtcag cgaggacggg
     2281 tatcctggct ggatgccgca gaaatggaca tggatacccc gtgagttacc cggcgggcgc
     2341 gcttggtgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2401 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     2461 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2521 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2581 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2641 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2701 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2761 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2821 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2881 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2941 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3001 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3061 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3121 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3181 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3241 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3301 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3361 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3421 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3481 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3541 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3601 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3661 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3721 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3781 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3841 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3901 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3961 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     4021 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     4081 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     4141 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     4201 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4261 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4321 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4381 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4441 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4501 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     4561 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     4621 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     4681 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     4741 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     4801 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     4861 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     4921 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     4981 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     5041 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     5101 acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt cacgtttttg aacccgtgga
     5161 ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg atggagcaga tgaagatgct
     5221 cgacacgctg cagaacacgc agctagatta accctagaaa gataatcata ttgtgacgta
     5281 cgttaaagat aatcatgtgt aaaattgacg catgtgtttt atcggtctgt atatcgaggt
     5341 ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca tactaataat
     5401 aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaacaa aaactcaaaa
     5461 tttcttctat aaagtaacaa aacttttatg agggacagcc cccccccaaa gcccccaggg
     5521 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     5581 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     5641 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     5701 cctgggggga tacggggaaa aggcctccac ggccactagt ctttcgtctt caagaattcc
     5761 tcgagtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat
     5821 agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc
     5881 cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc
     5941 tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat
     6001 agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag
     6061 tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc aactttccgt
     6121 accacttcct accctcgtaa aggtctagag ctagccatgg tgagcaaggg cgaggagctg
     6181 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     6241 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     6301 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     6361 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     6421 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     6481 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     6541 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     6601 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     6661 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     6721 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     6781 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     6841 gggatcactc tcggcatgga cgagctgtac aaggcgatga agatggacaa aaagaccatc
     6901 gtctggtttc ggagagattt gagaatagaa gataatcccg cgctcgccgc cgcggcccac
     6961 gagggttccg tcttccccgt tttcatttgg tgtcctgaag aagaaggcca gttttatccc
     7021 ggaagggcct ctaggtggtg gatgaagcaa agtctggccc atcttagcca gtcactgaaa
     7081 gcactgggca gtgatcttac cctgatcaag acacacaata ccatctctgc catcctcgac
     7141 tgcatcaggg tgaccggcgc aacgaaagtc gtgtttaacc acctgtacga tccagttagt
     7201 ctggtgcgcg accacactgt gaaggagaag ctggtggaac gggggatcag tgtgcagagc
     7261 tacaacgggg accttctgta cgagccatgg gagatctatt gcgagaaagg gaaaccgttc
     7321 acctccttca acagttactg gaagaaatgt ttggatatgt caatagagtc cgttatgttg
     7381 ccccctccct ggagactgat gccgattact gctgctgcag aggccatctg ggcctgctcc
     7441 atcgaggaac tcggtctgga aaatgaagca gaaaagccaa gcaatgcact tctcactaga
     7501 gcctggagcc ccggctggtc taatgccgac aagctgctta acgagttcat cgaaaaacaa
     7561 ctgattgact acgcgaagaa ctccaagaaa gtggtaggta actcaactag cttgctctct
     7621 ccatatctcc attttggcga gatttctgtc cgccatgtat ttcagtgcgc tcggatgaaa
     7681 cagattatct gggctcgcga taaaaacagc gaaggcgaag aaagcgccga tctgttcctg
     7741 cgagggatcg gacttcggga atactcccgg tatatatgtt tcaactttcc attcacacac
     7801 gagcagagtc tgttgtccca cctcaggttc ttcccctggg acgccgatgt cgacaaattc
     7861 aaggcatgga gacagggaag gacagggtac ccactcgtgg atgctggcat gagagagctc
     7921 tgggctacag gctggatgca caaccgcatc cgggtaatcg tgtcctcatt tgctgtcaag
     7981 tttctgctcc tgccttggaa atggggaatg aagtactttt gggataccct tctcgacgcc
     8041 gacttggagt gtgacattct gggatggcaa tatattagcg ggtcaattcc tgacggccat
     8101 gagttggaca ggttggacaa tccggccttg cagggagcta agtatgatcc cgaaggagag
     8161 tatattcgac agtggctccc cgagctggcc cgacttccta cggagtggat tcaccatcct
     8221 tgggacgcac cactgacagt gctcaaggca agcggggtgg agctgggcac caattacgct
     8281 aagcctatag ttgatataga tacagcacgc gagctgctgg ctaaagcgat ctctcgcact
     8341 cgggaggcgc agattatgat cggtgctgcc gagcctcagg aggagtctga agaagaagta
     8401 gaggaacctg aagaaagaca gcaaacacct gaggtggtac ctgatgattc tggaactttc
     8461 tatgatcagg cagttgtcag taatgacatg gaagaacatt tagaggagcc tgttgctgaa
     8521 ccagagcctg atcctgaacc agaaccagaa caagaacctg tatctgaaat ccaagaggaa
     8581 aagcctgagc cagtattaga agaaactgcc cctgaggatg ctcagaagag ttcttctcca
     8641 gcacctgcag acatagctca gacagtacag gaagacttga ggacattttc ttgggcatct
     8701 gtgaccagta agaatcttcc acccagtgga gctgttccag ttactgggat accacctcat
     8761 gttgttaaag taccagcttc acagccccgt ccagagtcta agcctgaatc tcagattcca
     8821 ccacaaagac ctcagcggga tcaaagagtg cgagaacaac gaataaatat tcctccccaa
     8881 aggggaccca gaccaatccg tgaggctggt gagcaaggtg acattgaacc ccgaagaatg
     8941 gtgagacacc ctgacagtca ccaactcttc attggcaacc tgcctcatga agtggacaaa
     9001 tcagagctta aagatttctt tcaaagttat ggaaacgtgg tggagttgcg cattaacagt
     9061 ggtgggaaat tacccaattt tggttttgtt gtgtttgatg attctgagcc tgttcagaaa
     9121 gtccttagca acaggcccat catgttcaga ggtgaggtcc gtctgaatgt cgaagagaag
     9181 aagactcgag ctgccaggga aggcgaccga cgagataatc gccttcgggg acctggaggc
     9241 cctcgaggtg ggctgggtgg tggaatgaga ggccctcccc gtggaggcat ggtgcagaaa
     9301 ccaggatttg gagtgggaag ggggcttgcg ccacggcagt gagaattcac tagtggatcc
     9361 ggcgcgcctc tagagcggcc gcatatatac cggttagtaa tgagtttaaa cgggggaggc
     9421 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
     9481 gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     9541 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     9601 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     9661 ggggcggcag gccctgccat agcagatctg cgaaatcgga tccagctgaa tctaagtgga
     9721 tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     9781 agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact
     9841 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     9901 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc
     9961 tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc
    10021 atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt
    10081 ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt
    10141 ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct
    10201 acgtctttgt ttcgttttct gttctgcgcc gttacagatc taagctgtga ccggcgccta
    10261 ctctagagcc gccatgtcta gactggacaa gagcaaagtc ataaactctg ctctggaatt
    10321 actcaatgga gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
    10381 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc
    10441 aatcgagatg ctggacaggc atcataccca ctcctgcccc ctggaaggcg agtcatggca
    10501 agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg
    10561 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
    10621 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
    10681 cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga
    10741 ggaaagagag acacctacca ccgattctat gcccccactt ctgaaacaag caattgagct
    10801 gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
    10861 cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga
    10921 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
    10981 cgctcttgac gattttgacc ttgacatgct ccccggggag ggcagaggaa gtcttctaac
    11041 atgcggtgac gtggaggaga atcccggccc tgaattcatg aaaaagcctg aactcaccgc
    11101 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
    11161 ctcggagggc gaagaatctc gt
//
