LOCUS       sequence-490570-       11303 bp    DNA     circular SYN 24-OCT-2025
DEFINITION  Used for multi-site piggybac transposon insertion of luciferase 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11303)
  TITLE     Bartel lab plasmids 2025
REFERENCE   2  (bases 1 to 11303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DBJS-p8.32
FEATURES             Location/Qualifiers
     source          1..11303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(81..872,873..941))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     704..723
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(942..1046)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1072..1527)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1209..1230)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1421..1440
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1654..1676
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1668..1685
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1669..1685
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1815..1877
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        2323..2608
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          2888..3115
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             3289..3765
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             3775..4290
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     promoter        7857..8068
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             8081..8349
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      8377..8386
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8383..8781
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8814..8935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8851..8870)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8905..8924
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   9698..9732
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10223..10239)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10223..10239)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10236..10258)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10247..10263
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10271..10277,10278..10295,10296..10301))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10316..10337
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10454..10471)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10625..11213)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10705..10724)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
       61 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
      121 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
      181 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
      241 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
      301 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
      361 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
      421 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
      481 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
      541 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
      601 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
      661 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
      721 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
      781 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
      841 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
      901 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
      961 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1021 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     1081 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     1141 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     1201 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     1261 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     1321 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     1381 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     1441 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     1501 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     1561 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     1621 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     1681 ccagtgagcg cgcctcgttc attcacgttt ttgaacccgt ggaggacggg cagactcgcg
     1741 gtgcaaatgt gttttacagc gtgatggagc agatgaagat gctcgacacg ctgcagaaca
     1801 cgcagctaga ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg
     1861 tgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa
     1921 tagatattaa gttttattat atttacactt acatactaat aataaattca acaaacaatt
     1981 tatttatgtt tatttattta ttaaaaaaaa caaaaactca aaatttcttc tataaagtaa
     2041 caaaactttt atgagggaca gccccccccc aaagccccca gggatgtaat tacgtccctc
     2101 ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc gctccccccg
     2161 catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg cacgggatcg
     2221 ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg ggatacgggg
     2281 aaaaggcctc cacggccact agtctttcgt cttcaagaat tccgttacat aacttacggt
     2341 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc
     2401 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     2461 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     2521 gcccgcctgg cattgtgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     2581 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
     2641 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
     2701 tgcagcgatg ggggcggggg gggggggggg gcgcgcgccr ggsggggsgg gggggggggg
     2761 gggggggggs ggggsgrggs ggrgrggkgc ggcggcagcc aatcagagcg gcgcgctccg
     2821 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
     2881 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
     2941 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
     3001 ttctcctccg ggctgtaatt agctgagcaa gaggtaaggg tttaagggat ggttggttgg
     3061 tggggtatta atgtttaatt acctggagca cctgcctgaa atcacttttt ttcaggttgg
     3121 accggtagtt gccgggcggg ggagggcgcg tccggttttt ctcaggggac gttgaaatta
     3181 tttttgtaac gggagtcggg agaggacggg gcgtgccccg acgtgcgcgc gcgtcgtcct
     3241 ccccggcgct cctccacagc tcgctggctc ccgccgcgga aaggcgtcat gatcagtctg
     3301 attgcggcgt tagcggtaga ttacgttatc ggcatggaaa acgccatgcc gtggaacctg
     3361 cctgccgatc tcgcctggtt taaacgcaac accttaaata aacccgtgat tatgggccgc
     3421 catacctggg aatcaatcgg tcgtccgttg ccaggacgca aaaatattat cctcagcagt
     3481 caaccgagta cggacgatcg cgtaacgtgg gtgaagtcgg tggatgaagc catcgcggcg
     3541 tgtggtgacg taccagaaat catggtgatt ggcggcggtc gcgttattga acagttcttg
     3601 ccaaaagcgc aaaaactgta tctgacgcat atcgacgcag aagtggaagg cgacacccat
     3661 ttcccggatt acgagccgga tgactgggaa tcggtattca gcgaattcca cgatgctgat
     3721 gcgcagaact ctcacagcta ttgctttgag attctggagc ggcgaggctc cggaatggtc
     3781 ttcacactcg aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa
     3841 gtccttgaac agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg
     3901 atccaaagga ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc
     3961 ccgtatgaag gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg
     4021 taccctgtgg atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac
     4081 ggggttacgc cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc
     4141 gacggcaaaa agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag
     4201 cgcctgatca accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc
     4261 tggcggctgt gcgaacgcat tctggcgtaa gggccaggga agaactggct cggaaagagg
     4321 accccacatg ttgtagcttc aagtttaaac ggatccaacc tacaaacggg tggaggatca
     4381 ccccacccga cacttcacaa tcaaggggta caatacacaa gggtggagga acaccccacc
     4441 ctccagacac attacacaga aatccaatca aacagaagca ccatcagggc ttctgctacc
     4501 aaatttatct caaaaaacta caacaaggaa tcaccatcag ggattccctg tgcaatatac
     4561 gtcaaacgag ggccacgacg ggaggacgat cacgcctccc gaatatcggc atgtctggct
     4621 ttcgaattca gtgcgtggag catcagccca cgcagccaat cagagtcgaa tacaagtcga
     4681 ctttcgcgaa gagcatcagc cttcgcgcca ttcttacaca aaccacactc tcccctacag
     4741 gaacagcatc agcgttcctg cccagtaccc aactcaagaa aatttatgtc cccatgcagc
     4801 atcagcgcat gggccccaag aatacatccc caacaaaatc acatccgagc accaacaggg
     4861 ctcggagtgt tgtttcttgt ccaactggac aaaccctcca tggaccatca ggccatggac
     4921 tctcaccaac aagacaaaaa ctactcttct cgaagcagca tcagcgcttc gaaacactcg
     4981 agcatacatt gtgcctattt cttgggtgga cgatcacgcc acccatgctc tcacgaattt
     5041 caaaacacgg acaaggacga gcaccaccag ggctcgtcgt tccacgtcca atacgattac
     5101 ttacctttcg ggatcacgat cacggatccc gcagctacat cacttccact caggacattc
     5161 aagcatgcac gatcacggca tgctccacaa gtctcaacca cagaaactac caaatgggtt
     5221 cagcaccagc gaacccactc ctacctcaaa cctcttccca caaaactggc aagcaggatc
     5281 accgcttgcc cattccaaca taccaaatca aaaacaatta ctggtacagc atcagcgtac
     5341 cagcccacat ctctcactac tatcaaaaac caaaccgttc agcaacagcg aacggtacac
     5401 acggaaaaat caactggttt acaaatacga aagacgatca cgctttcgtc cagcgcaaac
     5461 tattacgaaa aacatccgac gggaagagca acagccttcc cgcggcggaa aacctcacaa
     5521 aaacacgaca aacggatgca cgaacacggc atccgccgac aacccacaaa cttacaacca
     5581 ggcaaacggt gcaggatcac cgcaccgtac atcaaacacc tcagatctca tttcgcgcgg
     5641 gatccagacc acctcccctg cgagctaagc tggacagcca atgacgggta agagagtgac
     5701 atttttcact aacctaagac aggagggccg tcagagctac tgcctaatcc aaagacgggt
     5761 aaaagtgata aaaatgtatc actccaacct aagacaggcg cagcttccga gggatttgaa
     5821 tcgatttcgc gcggatctca ggaccttggt ggacactgtg tacacctctg gattcattgt
     5881 ctctcacaga tgtgactgta taactttccc aggttctgtt tatggccaca tttaatatct
     5941 tcagctcttt ttgtggatat aaaatgtgca gatgcaattg tttgggtgat tcctaagcca
     6001 cttgaaatgt tagtcattgt tatttataca agattgaaaa tcttgtgtaa atcctgccat
     6061 ttaaaaagtt gtagcagatt gtttcctctt ccaaagtaaa attgctgtgc tttatggata
     6121 gtaagaatgg ccctagagtg ggagtcctga taacccaggc ctgtctgact actttgcctt
     6181 cttttgtagc atataggtga tgtttgctct tgtttttatt aatttatatg tatatttttt
     6241 taatttaaca tgaacaccct tagaaaatgt gtcctatcta tcttccaaat gcaatttgat
     6301 tgactgccca ttcaccaaaa ttatcctgaa ctcttctgca aaaatggata ttattagaaa
     6361 ttagaaaaaa attactaatt ttacacatta gattttattt tactattgga atctgatata
     6421 ctgtgtgctt gttttataaa attttgcttt taattaaata aaagctggaa gcaaagtata
     6481 accatatgat actatcatac tactgaaaca gatttcatac ctcagaatgt aaaagaactt
     6541 actgattatt ttcttcatcc aacttatgtt tttaaatgag gattattgat agtactcttg
     6601 gtttttatac cattcagatc actgaattta taaagtaccc atctagtact tgaaaaagta
     6661 aagtgttctg ccagatctta ggtatagagg accctaacac agtatatccc aagtgcactt
     6721 tctaatgttt ctgggtcctg aagaattaag atacaaatta attttactcc ataaacagac
     6781 tgttaattat aggagcctta attttttttt catagagatt tgtctaattg catctcaaaa
     6841 ttattctgcc ctccttaatt tgggaaggtt tgtgttttct ctggaatggt acatgtcttc
     6901 catgtatctt ttgaactggc aattgtctat ttatctttta tttttttaag tcagtatggt
     6961 ctaacactgg catgttcaaa gccacattat ttctagtcca aaattacaag taatcaaggg
     7021 tcattatggg ttaggcatta atgtttctat ctgattttgt gcaaaagctt caaattaaaa
     7081 cagctgcatt agaaaaagag gcgcttctcc cctcccctac acctaaaggt gtatttaaac
     7141 tatcttgtgt gattaactta tttagagatg ctgtaactta aaatagggga tatttaaggt
     7201 agcttcagct agcttttagg aaaatcactt tgtctaactc agaattattt ttaaaaagaa
     7261 atctggtctt gttagaaaac aaaattttat tttgtgctca tttaagtttc aaacttacta
     7321 ttttgacagt tattttgata acaatgacac tagaaaactt gactccattt catcattgtt
     7381 tctgcatgaa tatcatacaa atcagttagt ttttaggtca agggcttact atttctgggt
     7441 cttttgctac taagttcaca ttagaattag tgccagaatt ttaggaactt cagagatcgt
     7501 gtattgagat ttcttaaata atgcttcaga tattattgct ttattgcttt tttgtattgg
     7561 ttaaaactgt acatttaaaa ttgctatgtt actattttct acaattaata gtttgtctat
     7621 tttaaaataa attagttgtt aagagtctta atggtctgat gttgtgttct ttgtattaag
     7681 tacactaatg ttctcttttc tgtctaggag aagatagata gaagataact ctcctagtat
     7741 ctcatccatt cctagccttt aaggggctct atatgctaga gatttccaaa tttatttctt
     7801 caaatcggat ccagctgaat ctaagtggat ctgcgatcgc tccggtgccc gtcagtgggc
     7861 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     7921 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     7981 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     8041 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     8101 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     8161 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     8221 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     8281 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     8341 ttacagatct aagctgtgac cggcgcctac tctagagccg ccatggccaa gcctttgtct
     8401 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     8461 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     8521 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     8581 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     8641 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     8701 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     8761 ggttatgtgt gggagggctg aaatcaacct ctggattaca aaatttgtgg gtaaacttgt
     8821 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8881 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8941 tctggaattg actcaaatga tgtcaattag tctatcagaa gctcatctgg tctcccttcc
     9001 gggggacaag acatccctgt ttaatattta aacagcagtg ttcccaaact gggttcttat
     9061 atcccttgct ctggtcaacc aggttgcagg gtttcctgtc ctcacaggaa cgaagtccct
     9121 aaagaaacag tggcagccag gtttagcccc ggaattgact ggattccttt tttagggccc
     9181 attggtatgg ctttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga
     9241 agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc
     9301 tgccggctcg gggatgcggg gggagcgccg gaccggagcg gagccccggg cggctcgctg
     9361 ctgcccccta gcgggggagg gacgtaatta catccctggg ggctttgggg gggggctgtc
     9421 cctgatatct ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa
     9481 ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg
     9541 tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag
     9601 cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc
     9661 gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact
     9721 atctttctag ggttaatcta gctgcatcag gatcatatcg tcgggtcttt tttccggctc
     9781 agtcatcgcc caagctggcg ctatctgggc atcggggagg aagaagcccg tgccttttcc
     9841 cgcgaggttg aagcggcatg gaaagagttt gccgaggatg actgctgctg cattgacgtt
     9901 gagcgaaaac gcacgtttac catgatgatt cgggaaggtg tggccatgca cgcctttaac
     9961 ggtgaactgt tcgttcaggc cacctgggat accagttcgt cgcggctttt ccggacacag
    10021 ttccggatgg tcagcccgaa gcgcatcagc aacccgaaca ataccggcga cagccggaac
    10081 tgccgtgccg gtgtgcagat taatgacagc ggtgcggcgc tgggatatta cgtcagcgag
    10141 gacgggtatc ctggctggat gccgcagaaa tggacatgga taccccgtga gttacccggc
    10201 gggcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    10261 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    10321 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    10381 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    10441 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    10501 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    10561 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    10621 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    10681 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    10741 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    10801 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    10861 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    10921 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    10981 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    11041 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    11101 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    11161 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    11221 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    11281 tggtcatgag attatcaaaa agg
//
