LOCUS       sequence-490572-        9849 bp    DNA     circular SYN 24-OCT-2025
DEFINITION  Protein expression of yeast VPS15 (1-981)-ZZ in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Ohashi Y, Soler N, Garcia Ortegon M, Zhang L, Kirsten ML, Perisic O,
            Masson GR, Burke JE, Jakobi AJ, Apostolakis AA, Johnson CM, Ohashi 
            M, Ktistakis NT, Sachse C, Williams RL
  TITLE     Characterization of Atg38 and NRBF2, a fifth subunit of the 
            autophagic Vps34/PIK3C3 complex.
  JOURNAL   Autophagy. 2016 Nov;12(11):2129-2144. doi: 
            10.1080/15548627.2016.1226736. Epub 2016 Sep 14.
  PUBMED    27630019
REFERENCE   2  (bases 1 to 9849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15548627.2016.1226736"; journalName: "Autophagy"; date: 
            "2016-11"; volume: "12"; issue: "11"; pages: "2129-2144"
COMMENT     Sequence Label: pYO286
FEATURES             Location/Qualifiers
     source          1..9849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1966..1985
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1966..1984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2069..2186
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     2367..2390
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             5471..5491
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5513..5533
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5615..5635
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5651..5824
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             5825..5998
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     terminator      6021..6208
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6076..6100)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(6247..6267)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6247..6265)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6286..6302)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6286..6302)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6299..6321)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6310..6326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6334..6340,6341..6358,6359..6364))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6379..6400
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6517..6534)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6688..7276)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6768..6787)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7447..8238,8239..8307))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8070..8089
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8308..8412)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8439..9781
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9402..9449)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9822..9840
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     1981 tagggcgaat tgggtaccgc tatgacatga ttacgaattg gcgcgccggg ccgcggatct
     2041 ttgcaaagat ccaaaaaaag catgcgtacg gattagaagc cgccgagcgg gtgacagccc
     2101 tccgaaggaa gactctcctc cgtgcgtcct cgtcttcacc ggtcgcgttc ctgaaacgca
     2161 gatgtgcctc gcgccgcact gctccgaaca ataaagattc tacaatacta gcttttatgg
     2221 ttatgaagag gaaaattggc agtaacctgg ccccacaaac ctcaaatgaa cgaatcaaat
     2281 taacaaccag atatctcgac tgaaaaaaaa ggtttaaacc agttccctga aattattccc
     2341 ctacttgact aataagtata taaagacggt aggtattgat tgtaattctg taaatctatt
     2401 tcttaaactt cttgaattct acttttatag ttagtctttt ttttagtttt aaaacaccaa
     2461 gaacttagtt tcgaataaac acacataaat aaacctcgag atgggggcac aattatcact
     2521 agtggtccaa gcatcacctt ccatagccat tttttcatat atcgatgtct tagaggaagt
     2581 acactacgtt tcacagttaa actcatcaag attcttaaaa acatgcaagg cactggaccc
     2641 taacggcgaa attgttatca aagtgtttat taaaccaaaa gaccaatata gcttacgacc
     2701 ttttctccaa cgtataaggg ctcaatcgtt taagttggga caactaccgc acgttttaaa
     2761 ctacagtaaa ttgatcgaga caaatagagc cggctacatg atacggcagc acttaaaaaa
     2821 taatttatat gacagattga gtttgagacc ttacttacaa gacattgaac tgaaattcat
     2881 tgctttccag ttgttaaatg cattaaagga cattcataat ctgaatattg tccatggtga
     2941 tataaagaca gaaaatatcc tagtaacaag ttggaattgg tgtatattga cagattttgc
     3001 tgcatttatc aaacccgtat atttgcctga agataatcca ggtgaatttt tattctactt
     3061 cgacacctcg aagagaagaa cctgttatct agccccggag aggtttaact ctaaacttta
     3121 ccaagatgga aaatctaaca atggtaggct aactaaagaa atggacatat ttagtcttgg
     3181 atgtgttatt gcagaaatat ttgctgaagg aagacccatc ttcaacttat cacagctatt
     3241 caaatataaa agtaattcat atgacgtaaa cagggaattt ctcatggagg aaatgaattc
     3301 taccgattta aggaacttgg ttctagacat gattcaacta gatccatcca aaagactttc
     3361 atgtgatgaa ctactgaata aatatcgtgg cattttcttc cccgattatt tctacacttt
     3421 catttatgat tatttcagaa atttggttac tatgacaaca agcacaccga tatcagataa
     3481 cacttgcacc aatagtacct tggaagacaa tgtaaaactt ttagatgaaa ctacggaaaa
     3541 aatatacaga gatttttccc aaatatgtca ttgtttggac tttcctttaa taaaagacgg
     3601 gggtgagatt ggttcagacc ccccaatttt ggaatcttac aaaatagaga tagaaattag
     3661 tcggttttta aacacaaact tatatttccc ccaaaattac catttagtct tacagcagtt
     3721 taccaaagta tccgaaaaga taaaatcagt taaagaggaa tgtgccttac tctttatctc
     3781 ttatttgtct catagtataa gaagtattgt ttcgactgct acgaaactta aaaatttaga
     3841 actattagca gtatttgcac aattcgtttc tgatgaaaat aaaattgatc gagtcgtacc
     3901 ttatttcgta tgttgttttg aagatagtga ccaggacgtc caggccctat ctttgttaac
     3961 attaatccag gtactcacct ctgtaagaaa attgaatcaa ttgaacgaga atatatttgt
     4021 ggactactta cttccgagac tgaaaagatt acttatttcc aataggcaga ataccaatta
     4081 tttaaggatt gtgtttgcta attgtttgag cgacttggcc attatcatta atagatttca
     4141 agaatttaca tttgctcagc actgcaatga taactcaatg gataacaaca cggaaatcat
     4201 ggaaagcagt accaagtatt cagcaaaatt gatccaaagt gtcgaagatt taactgtctc
     4261 ttttttaaca gataatgata cttatgtaaa gatggcactt ttgcaaaaca ttcttccact
     4321 ttgtaaattt tttggtaggg aaagaacaaa cgatattata ctgagtcatt taataaccta
     4381 ccttaatgat aaggacccag cattgcgagt ttccttaatt caaacaatat ccggaatatc
     4441 aattcttttg ggtaccgtta cattagaaca gtatatttta ccattgttaa tccagaccat
     4501 cactgattcg gaagaattag tagtgatcag tgttttacaa agcttaaaat ctttgttcaa
     4561 gactgggttg attaggaaaa aatattatat tgatatatca aaaacaacat ctcccttgtt
     4621 gttgcatcct aataattgga taagacagtt tactttgatg ataattatag aaattattaa
     4681 taagttatca aaagccgaag tgtactgcat tctctatcca ataataaggc ctttcttcga
     4741 atttgacgtt gagttcaact tcaaatcaat gataagctgt tgcaagcaac cagtgtcaag
     4801 atcggtttac aatctattgt gtagttggtc tgttagagcg tcaaaatctt tattttggaa
     4861 aaaaatcatc acaaatcatg tagattcatt cggaaataat agaatcgaat ttataacaaa
     4921 aaattactca agtaaaaatt atggatttaa taaaagagat acgaaatcaa gttcctcgct
     4981 gaagggtatt aaaacatcat ccaccgtcta ttcacatgac aacaaggaaa ttcccttaac
     5041 tgctgaagac agaaattgga ttgataagtt ccacattatt gggctaacag aaaaagatat
     5101 ttggaaaatt gtggctttga ggggttatgt aataaggaca gcgagagtta tggcagcgaa
     5161 ccctgatttt ccatataata atagtaatta ccgtccatta gtacagaact caccacctaa
     5221 cctaaacctt acgaatatta tgccaagaaa cattttcttt gatgtagagt ttgctgaaga
     5281 gtcgacaagt gagggacaag attctaactt agagaaccaa cagatatata aatatgatga
     5341 aagtgagaaa gatagcaata agctaaatat taacggtagc aaacagctat ctaccgtcat
     5401 ggacataaat ggatcactaa tattcaaaaa taagtccatt gccggatcct ctagaccaac
     5461 tactgcctct gaaaatttgt actttcaagg cgagttgaag accactgctt ctgaaaactt
     5521 gtacttccaa ggtgagttac tcgagggtga attgaagact gctgctttgg ctcaacacga
     5581 cgaagctaga tccggagcag gggcgggtgc tagcgagaac ctgtacttcc agggcgccca
     5641 cgatgaagcc gtagacaaca aattcaacaa agaacaacaa aacgcgttct atgagatctt
     5701 acatttacct aacttaaacg aagaacaacg aaacgccttc atccaaagtt taaaagatga
     5761 cccaagccaa agcgctaacc ttttagcaga agctaaaaag ctaaatgatg ctcaggcgcc
     5821 gaaagtagac aacaaattca acaaagaaca acaaaacgcg ttctatgaga tcttacattt
     5881 acctaactta aacgaagaac aacgaaacgc cttcatccaa agtttaaaag atgacccaag
     5941 ccaaagcgct aaccttttag cagaagctaa aaagctaaat gatgctcagg cgccgaaagt
     6001 agacgcgaat tcgtgagctc gcgaatttct tatgatttat gatttttatt attaaataag
     6061 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
     6121 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg
     6181 tcgctcttat tgaccacacc tctaccggca gcggccgcca ccgcggtgga gctccagctt
     6241 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     6301 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6361 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6421 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6481 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6541 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6601 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6661 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6721 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6781 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6841 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6901 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6961 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7021 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7081 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7141 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7201 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7261 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7321 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7381 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7441 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7501 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7561 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7621 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7681 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7741 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7801 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7861 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7921 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7981 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8041 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8101 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8161 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8221 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8281 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8341 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8401 aggggttccg cgcacatttc cccgaaaagt gccacctgaa cgaagcatct gtgcttcatt
     8461 ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
     8521 tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt
     8581 catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
     8641 ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta
     8701 tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc
     8761 ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc
     8821 actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa
     8881 aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt
     8941 tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga
     9001 acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct
     9061 attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca
     9121 ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat
     9181 aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt
     9241 tatataggga tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg
     9301 aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga
     9361 aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta
     9421 gagaatagga acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa
     9481 atgcaacgcg agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt
     9541 gcctgtatat atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgta
     9601 cttatatgcg tctatttatg taggatgaaa ggtagtctag tacctcctgt gatattatcc
     9661 cattccatgc ggggtatcgt atgcttcctt cagcactacc ctttagctgt tctatatgct
     9721 gccactcctc aattggatta gtctcatcct tcaatgctat catttccttt gatattggat
     9781 catactaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     9841 cctttcgtc
//
