LOCUS       sequence-490575-       10574 bp    DNA     circular SYN 24-OCT-2025
DEFINITION  Used for multi-site piggybac transposon insertion of CRY2-tagged 
            G3BP1 for overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10574)
  TITLE     Bartel lab plasmids 2025
REFERENCE   2  (bases 1 to 10574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DBJS-p8.55
FEATURES             Location/Qualifiers
     source          1..10574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     11..30
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(249..353)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(379..834)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(516..537)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     728..747
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     961..983
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     975..992
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     976..992
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1122..1184
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1633..2011
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1641..1659
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1677..1695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1713..1731
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1749..1767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1785..1803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1821..1839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1857..1875
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1920..1944
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3542..3551
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3548..3550,3551..3553,3554..4264)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3548..3550,3551..3553,3554..4264)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3593..3614)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3854..3873)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4201..4222
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5322..5341)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5366..5414
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5645..5856
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5869..6137
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6171..6914
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    7392..7513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7429..7448)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7483..7502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8276..8310
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8801..8817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8801..8817)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8814..8836)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8825..8841
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8849..8855,8856..8873,8874..8879))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8894..8915
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9032..9049)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9203..9791)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9283..9302)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9962..10574,1..179,180..248))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTENHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
       61 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
      121 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      181 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      241 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      301 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      361 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
      421 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
      481 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
      541 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
      601 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
      661 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
      721 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
      781 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
      841 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
      901 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
      961 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt cacgtttttg
     1021 aacccgtgga ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg atggagcaga
     1081 tgaagatgct cgacacgctg cagaacacgc agctagatta accctagaaa gataatcata
     1141 ttgtgacgta cgttaaagat aatcatgtgt aaaattgacg catgtgtttt atcggtctgt
     1201 atatcgaggt ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca
     1261 tactaataat aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaacaa
     1321 aaactcaaaa tttcttctat aaagtaacaa aacttttatg agggacagcc cccccccaaa
     1381 gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg
     1441 ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc
     1501 gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag
     1561 cctgcagaca cctgggggga tacggggaaa aggcctccac ggccactagt ctttcgtctt
     1621 caagaattcc tcgagtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc
     1681 tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag
     1741 gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag
     1801 agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc
     1861 tatcagtgat agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga
     1921 gctcgtttag tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc
     1981 aactttccgt accacttcct accctcgtaa aggtctagag ctagccatga agatggacaa
     2041 aaagaccatc gtctggtttc ggagagattt gagaatagaa gataatcccg cgctcgccgc
     2101 cgcggcccac gagggttccg tcttccccgt tttcatttgg tgtcctgaag aagaaggcca
     2161 gttttatccc ggaagggcct ctaggtggtg gatgaagcaa agtctggccc atcttagcca
     2221 gtcactgaaa gcactgggca gtgatcttac cctgatcaag acacacaata ccatctctgc
     2281 catcctcgac tgcatcaggg tgaccggcgc aacgaaagtc gtgtttaacc acctgtacga
     2341 tccagttagt ctggtgcgcg accacactgt gaaggagaag ctggtggaac gggggatcag
     2401 tgtgcagagc tacaacgggg accttctgta cgagccatgg gagatctatt gcgagaaagg
     2461 gaaaccgttc acctccttca acagttactg gaagaaatgt ttggatatgt caatagagtc
     2521 cgttatgttg ccccctccct ggagactgat gccgattact gctgctgcag aggccatctg
     2581 ggcctgctcc atcgaggaac tcggtctgga aaatgaagca gaaaagccaa gcaatgcact
     2641 tctcactaga gcctggagcc ccggctggtc taatgccgac aagctgctta acgagttcat
     2701 cgaaaaacaa ctgattgact acgcgaagaa ctccaagaaa gtggtaggta actcaactag
     2761 cttgctctct ccatatctcc attttggcga gatttctgtc cgccatgtat ttcagtgcgc
     2821 tcggatgaaa cagattatct gggctcgcga taaaaacagc gaaggcgaag aaagcgccga
     2881 tctgttcctg cgagggatcg gacttcggga atactcccgg tatatatgtt tcaactttcc
     2941 attcacacac gagcagagtc tgttgtccca cctcaggttc ttcccctggg acgccgatgt
     3001 cgacaaattc aaggcatgga gacagggaag gacagggtac ccactcgtgg atgctggcat
     3061 gagagagctc tgggctacag gctggatgca caaccgcatc cgggtaatcg tgtcctcatt
     3121 tgctgtcaag tttctgctcc tgccttggaa atggggaatg aagtactttt gggataccct
     3181 tctcgacgcc gacttggagt gtgacattct gggatggcaa tatattagcg ggtcaattcc
     3241 tgacggccat gagttggaca ggttggacaa tccggccttg cagggagcta agtatgatcc
     3301 cgaaggagag tatattcgac agtggctccc cgagctggcc cgacttccta cggagtggat
     3361 tcaccatcct tgggacgcac cactgacagt gctcaaggca agcggggtgg agctgggcac
     3421 caattacgct aagcctatag ttgatataga tacagcacgc gagctgctgg ctaaagcgat
     3481 ctctcgcact cgggaggcgc agattatgat cggtgctgcc ggcagcggca gcccaccggt
     3541 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3601 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     3661 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     3721 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     3781 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3841 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3901 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3961 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     4021 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     4081 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     4141 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     4201 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     4261 caaggcggag cctcaggagg agtctgaaga agaagtagag gaacctgaag aaagacagca
     4321 aacacctgag gtggtacctg atgattctgg aactttctat gatcaggcag ttgtcagtaa
     4381 tgacatggaa gaacatttag aggagcctgt tgctgaacca gagcctgatc ctgaaccaga
     4441 accagaacaa gaacctgtat ctgaaatcca agaggaaaag cctgagccag tattagaaga
     4501 aactgcccct gaggatgctc agaagagttc ttctccagca cctgcagaca tagctcagac
     4561 agtacaggaa gacttgagga cattttcttg ggcatctgtg accagtaaga atcttccacc
     4621 cagtggagct gttccagtta ctgggatacc acctcatgtt gttaaagtac cagcttcaca
     4681 gccccgtcca gagtctaagc ctgaatctca gattccacca caaagacctc agcgggatca
     4741 aagagtgcga gaacaacgaa taaatattcc tccccaaagg ggacccagac caatccgtga
     4801 ggctggtgag caaggtgaca ttgaaccccg aagaatggtg agacaccctg acagtcacca
     4861 actcttcatt ggcaacctgc ctcatgaagt ggacaaatca gagcttaaag atttctttca
     4921 aagttatgga aacgtggtgg agttgcgcat taacagtggt gggaaattac ccaattttgg
     4981 ttttgttgtg tttgatgatt ctgagcctgt tcagaaagtc cttagcaaca ggcccatcat
     5041 gttcagaggt gaggtccgtc tgaatgtcga agagaagaag actcgagctg ccagggaagg
     5101 cgaccgacga gataatcgcc ttcggggacc tggaggccct cgaggtgggc tgggtggtgg
     5161 aatgagaggc cctccccgtg gaggcatggt gcagaaacca ggatttggag tgggaagggg
     5221 gcttgcgcca cggcagtgag aattcactag tggatccggc gcgcctctag agcggccgca
     5281 tatataccgg ttagtaatga gtttaaacgg gggaggctaa ctgaaacacg gaaggagaca
     5341 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt
     5401 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     5461 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     5521 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     5581 agatctgcga aatcggatcc agctgaatct aagtggatct gcgatcgctc cggtgcccgt
     5641 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     5701 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     5761 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     5821 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     5881 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     5941 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     6001 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     6061 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     6121 ctgcgccgtt acagatctaa gctgtgaccg gcgcctactc tagagccgcc atgtctagac
     6181 tggacaagag caaagtcata aactctgctc tggaattact caatggagtc ggtatcgaag
     6241 gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc ctgtactggc
     6301 acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg gacaggcatc
     6361 atacccactc ctgccccctg gaaggcgagt catggcaaga ctttctgcgg aacaacgcca
     6421 agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat ctcggcaccc
     6481 gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag
     6541 gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt acactgggct
     6601 gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca cctaccaccg
     6661 attctatgcc cccacttctg aaacaagcaa ttgagctgtt cgaccggcag ggagccgaac
     6721 ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag ctaaagtgcg
     6781 aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc ccagccgatg
     6841 cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat tttgaccttg
     6901 acatgctccc cggggagggc agaggaagtc ttctaacata cggtgacgtg gaggagaatc
     6961 ccggccctga attcatggcc aagctaagaa gaatccaccc tcattgaaag agcaacggct
     7021 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
     7081 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa
     7141 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
     7201 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc
     7261 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
     7321 gggattcgtg aatcgctgcc ctctggttat gtgtgggagg gctgacctct ggattacaaa
     7381 atttgtgggt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7441 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7501 tcaatgtatc ttatcatgtc tggaattgac tcaaatgatg tcaattagtc tatcagaagc
     7561 tcatctggtc tcccttccgg gggacaagac atccctgttt aatatttaaa cagcagtgtt
     7621 cccaaactgg gttcttatat cccttgctct ggtcaaccag gttgcagggt ttcctgtcct
     7681 cacaggaacg aagtccctaa agaaacagtg gcagccaggt ttagccccgg aattgactgg
     7741 attccttttt tagggcccat tggtatggct ttttccccgt atccccccag gtgtctgcag
     7801 gctcaaagag cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc
     7861 cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga
     7921 gccccgggcg gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg
     7981 ctttgggggg gggctgtccc tgatatctat aacaagaaaa tatatatata ataagttatc
     8041 acgtaagtag aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac
     8101 gtaaaagata atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac
     8161 acttaccgca ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa
     8221 atgcacagcg acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc
     8281 gtcaatttta cgcagactat ctttctaggg ttaatctagc tgcatcagga tcatatcgtc
     8341 gggtcttttt tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa
     8401 gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac
     8461 tgctgctgca ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg
     8521 gccatgcacg cctttaacgg tgaactgttc gttcaggcca cctgggatac cagttcgtcg
     8581 cggcttttcc ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat
     8641 accggcgaca gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg
     8701 ggatattacg tcagcgagga cgggtatcct ggctggatgc cgcagaaatg gacatggata
     8761 ccccgtgagt tacccggcgg gcgcgcttgg tgtaatcatg gtcatagctg tttcctgtgt
     8821 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     8881 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     8941 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     9001 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     9061 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     9121 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9181 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9241 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9301 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9361 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9421 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9481 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9541 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9601 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     9661 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9721 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9781 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9841 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9901 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9961 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    10021 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    10081 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    10141 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    10201 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    10261 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10321 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10381 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10441 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgatttt
    10501 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10561 gctcttgccc ggcg
//
