LOCUS       sequence-490586-        9857 bp    DNA     circular SYN 24-OCT-2025
DEFINITION  Used for multi-site piggybac transposon insertion of luciferase 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9857)
  TITLE     Bartel lab plasmids 2025
REFERENCE   2  (bases 1 to 9857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DBJS-p8.35
FEATURES             Location/Qualifiers
     source          1..9857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(103..122)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      916..1504
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1405..1424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1658..1675
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1792..1813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1828..1833,1834..1851,1852..1858)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1866..1882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1871..1893
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1890..1906
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1890..1906
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   2397..2431
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    3194..3315
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3205..3224)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3259..3278
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3348..3746)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      3743..3752
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             3780..4048
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(4061..4272)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             complement(6284..6799)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             complement(6809..7285)
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     intron          7570..7797
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     enhancer        8075..8360
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     repeat_region   8806..8868
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8998..9015)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8998..9014)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9007..9029)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9156..9611
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9243..9262)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9453..9474
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9637..9741
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9742..9810,9811..9857,1..745)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
       61 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      121 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      181 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      241 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      301 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      361 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      421 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      481 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      541 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      601 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      661 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      721 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
      781 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
      841 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
      901 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
      961 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1021 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1081 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1141 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1201 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1261 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1321 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1381 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1441 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1501 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1561 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1621 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1681 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1741 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     1801 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1861 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1921 gcgcgcccgc cgggtaactc acggggtatc catgtccatt tctgcggcat ccagccagga
     1981 tacccgtcct cgctgacgta atatcccagc gccgcaccgc tgtcattaat ctgcacaccg
     2041 gcacggcagt tccggctgtc gccggtattg ttcgggttgc tgatgcgctt cgggctgacc
     2101 atccggaact gtgtccggaa aagccgcgac gaactggtat cccaggtggc ctgaacgaac
     2161 agttcaccgt taaaggcgtg catggccaca ccttcccgaa tcatcatggt aaacgtgcgt
     2221 tttcgctcaa cgtcaatgca gcagcagtca tcctcggcaa actctttcca tgccgcttca
     2281 acctcgcggg aaaaggcacg ggcttcttcc tccccgatgc ccagatagcg ccagcttggg
     2341 cgatgactga gccggaaaaa agacccgacg atatgatcct gatgcagcta gattaaccct
     2401 agaaagatag tctgcgtaaa attgacgcat gcattcttga aatattgctc tctctttcta
     2461 aatagcgcga atccgtcgct gtgcatttag gacatctcag tcgccgcttg gagctcccgt
     2521 gaggcgtgct tgtcaatgcg gtaagtgtca ctgattttga actataacga ccgcgtgagt
     2581 caaaatgacg catgattatc ttttacgtga cttttaagat ttaactcata cgataattat
     2641 attgttattt catgttctac ttacgtgata acttattata tatatatttt cttgttatag
     2701 atatcaggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
     2761 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
     2821 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
     2881 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagcc
     2941 ataccaatgg gccctaaaaa aggaatccag tcaattccgg ggctaaacct ggctgccact
     3001 gtttctttag ggacttcgtt cctgtgagga caggaaaccc tgcaacctgg ttgaccagag
     3061 caagggatat aagaacccag tttgggaaca ctgctgttta aatattaaac agggatgtct
     3121 tgtcccccgg aagggagacc agatgagctt ctgatagact aattgacatc atttgagtca
     3181 attccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     3241 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     3301 tgcaataaac aagtttaccc acaaattttg taatccagag gttgatttca gccctcccac
     3361 acataaccag agggcagcaa ttcacgaatc ccaactgccg tcggctgtcc atcactgtcc
     3421 ttcactatgg ctttgatccc aggatgcaga tcgagaagca cctgtcggca ccgtccgcag
     3481 gggctcaaga tgcccctgtt ctcatttccg atcgcgacga tacaagtcag gttgccagct
     3541 gccgcagcag cagcagtgcc cagcaccacg agttctgcac aaggtccccc agtaaaatga
     3601 tatacattga caccagtgaa gatgcggccg tcgctagaga gagctgcgct ggcgacgctg
     3661 tagtcttcag agatggggat gctgttgatt gtagccgttg ctctttcaat gagggtggat
     3721 tcttcttgag acaaaggctt ggccatggcg gctctagagt aggcgccggt cacagcttag
     3781 atctgtaacg gcgcagaaca gaaaacgaaa caaagacgta gagttgagca agcagggtca
     3841 ggcaaagcgt ggagagccgg ctgagtctag gtaggctcca agggagcgcc ggacaaaggc
     3901 ccggtctcga cctgagcttt aaacttacct agacggcgga cgcagttcag gaggcaccac
     3961 aggcgggagg cggcagaacg cgactcaacc ggcgtggatg gcggcctcag gtagggcggc
     4021 gggcgcgtga aggagagatg cgagcccctc gaagcttcag ctgtgttctg gcggcaaacc
     4081 cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct
     4141 cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc
     4201 tctaggcacc cgttcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg
     4261 tgcgctctgc ccactgacgg gcaccggagc gatcgcagat ccacttagat tcagctggat
     4321 ccgatttacg tcaactctct ttccctcccc ttcaccccca ggtcagcttc tgaactggct
     4381 attttatcta ctgctgatgt tctttgtcaa tttttgaacg ttaacacaaa acctcagatg
     4441 ctaaaataat ctaatagtca ataaaagtgt aaaagacttc tgataaaatt atgttttaat
     4501 tgcctatggc atatattaca taatggctca aaattaatag tctggctgaa attaagaaaa
     4561 acagctgatg atgacttagt aaatccttaa ttttaagtaa gcttttggcc aaaagtcagg
     4621 ggtagaattg actcaaaatt aacagccttc accatctttt attcattctg ctgtgataca
     4681 actaaaatgg ccagtaaatt ctcccctggg tctcaggtaa cagttttcca aaagtgaagt
     4741 agcgcgaaat cgattcaaat ccctcggaag ctgcgcctgt cttaggttgg agtgatacat
     4801 ttttatcact tttacccgtc tttggattag gcagtagctc tgacggccct cctgtcttag
     4861 gttagtgaaa aatgtcactc tcttacccgt cattggctgt ccagcttagc tcgcagggga
     4921 ggtggtctgg atcccgcgcg aaatgagatc tgaggtgttt gatgtacggt gcggtgatcc
     4981 tgcaccgttt gcctggttgt aagtttgtgg gttgtcggcg gatgccgtgt tcgtgcatcc
     5041 gtttgtcgtg tttttgtgag gttttccgcc gcgggaaggc tgttgctctt cccgtcggat
     5101 gtttttcgta atagtttgcg ctggacgaaa gcgtgatcgt ctttcgtatt tgtaaaccag
     5161 ttgatttttc cgtgtgtacc gttcgctgtt gctgaacggt ttggtttttg atagtagtga
     5221 gagatgtggg ctggtacgct gatgctgtac cagtaattgt ttttgatttg gtatgttgga
     5281 atgggcaagc ggtgatcctg cttgccagtt ttgtgggaag aggtttgagg taggagtggg
     5341 ttcgctggtg ctgaacccat ttggtagttt ctgtggttga gacttgtgga gcatgccgtg
     5401 atcgtgcatg cttgaatgtc ctgagtggaa gtgatgtagc tgcgggatcc gtgatcgtga
     5461 tcccgaaagg taagtaatcg tattggacgt ggaacgacga gccctggtgg tgctcgtcct
     5521 tgtccgtgtt ttgaaattcg tgagagcatg ggtggcgtga tcgtccaccc aagaaatagg
     5581 cacaatgtat gctcgagtgt ttcgaagcgc tgatgctgct tcgagaagag tagtttttgt
     5641 cttgttggtg agagtccatg gcctgatggt ccatggaggg tttgtccagt tggacaagaa
     5701 acaacactcc gagccctgtt ggtgctcgga tgtgattttg ttggggatgt attcttgggg
     5761 cccatgcgct gatgctgcat ggggacataa attttcttga gttgggtact gggcaggaac
     5821 gctgatgctg ttcctgtagg ggagagtgtg gtttgtgtaa gaatggcgcg aaggctgatg
     5881 ctcttcgcga aagtcgactt gtattcgact ctgattggct gcgtgggctg atgctccacg
     5941 cactgaattc gaaagccaga catgccgata ttcgggaggc gtgatcgtcc tcccgtcgtg
     6001 gccctcgttt gacgtatatt gcacagggaa tccctgatgg tgattccttg ttgtagtttt
     6061 ttgagataaa tttggtagca gaagccctga tggtgcttct gtttgattgg atttctgtgt
     6121 aatgtgtctg gagggtgggg tgttcctcca cccttgtgta ttgtacccct tgattgtgaa
     6181 gtgtcgggtg gggtgatcct ccacccgttt gtaggttgga tccgtttaaa cttgaagcta
     6241 caacatgtgg ggtcctcttt ccgagccagt tcttccctgg cccttacgcc agaatgcgtt
     6301 cgcacagccg ccagccggtc actccgttga tggttactcg gaacagcagg gagccgtcgg
     6361 ggttgatcag gcgctcgtcg ataattttgt tgccgttcca cagggtccct gttacagtga
     6421 tctttttgcc gtcgaacacg gcgatgcctt catacggccg tccgaaatag tcgatcatgt
     6481 tcggcgtaac cccgtcgatt accagtgtgc catagtgcag gatcacctta aagtgatgat
     6541 catccacagg gtacaccacc ttaaaaattt tttcgatctg gcccatttgg tcgccgctca
     6601 gaccttcata cgggatgatg acatggatgt cgatcttcag cccattttca ccgctcagga
     6661 caatcctttg gatcggagtt acggacaccc cgagattctg aaacaaactg gacacacctc
     6721 cctgttcaag gacttggtcc aggttgtagc cggctgtctg tcgccagtcc ccaacgaaat
     6781 cttcgagtgt gaagaccatt ccggagcctc gccgctccag aatctcaaag caatagctgt
     6841 gagagttctg cgcatcagca tcgtggaatt cgctgaatac cgattcccag tcatccggct
     6901 cgtaatccgg gaaatgggtg tcgccttcca cttctgcgtc gatatgcgtc agatacagtt
     6961 tttgcgcttt tggcaagaac tgttcaataa cgcgaccgcc gccaatcacc atgatttctg
     7021 gtacgtcacc acacgccgcg atggcttcat ccaccgactt cacccacgtt acgcgatcgt
     7081 ccgtactcgg ttgactgctg aggataatat ttttgcgtcc tggcaacgga cgaccgattg
     7141 attcccaggt atggcggccc ataatcacgg gtttatttaa ggtgttgcgt ttaaaccagg
     7201 cgagatcggc aggcaggttc cacggcatgg cgttttccat gccgataacg taatctaccg
     7261 ctaacgccgc aatcagactg atcatcttcc cgcttcgact cgggttcttc cctgcaggtg
     7321 gcgtggccct gactcccgcc gccgggctgg ccgctgtcct ggacctccga ggacaactct
     7381 gtgcctaccc cggctccggg cccccaggtt ccggctagag gagtgggcag acggcgggct
     7441 gaggaagtga cgagagctag ttcccgtcgt ctagcaacag caacagcttc cccaccaagg
     7501 gaccgccaca gcctcccgcc atacacagct tccgcctcac ggcgtcccgg tacccactac
     7561 cggtccaacc tgaaaaaaag tgatttcagg caggtgctcc aggtaattaa acattaatac
     7621 cccaccaacc aaccatccct taaaccctta cctcttgctc agctaattac agcccggagg
     7681 agaagggccg tcccgcccgc tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg
     7741 gcggcgcgag gcggcggcgg agcggggcac ggggcgaagg cagcgcgcag cgactcccgc
     7801 ccgccgcgcg cttcgctttt tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa
     7861 ctttcggagc gcgccgctct gattggctgc cgccscmccy cyccsccycc cccccccccc
     7921 cccccccccc cccccccccc ccccyggcgc gccccccccc cccccccgcc cccatcgctg
     7981 cacaaaataa ttaaaaaata aataaataca aaattggggg tggggagggg ggggagatgg
     8041 ggagagtgaa gcagaacgtg gggctcacct cgaccatggt aatagcgatg actaatacgt
     8101 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcacaa tgccaggcgg
     8161 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta
     8221 ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     8281 ttggcgttac tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     8341 ttaccgtaag ttatgtaacg gaattcttga agacgaaaga ctagtggccg tggaggcctt
     8401 ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa
     8461 agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga
     8521 tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg
     8581 gggagggacg taattacatc cctgggggct ttgggggggg gctgtccctc ataaaagttt
     8641 tgttacttta tagaagaaat tttgagtttt tgtttttttt aataaataaa taaacataaa
     8701 taaattgttt gttgaattta ttattagtat gtaagtgtaa atataataaa acttaatatc
     8761 tattcaaatt aataaataaa cctcgatata cagaccgata aaacacatgc gtcaatttta
     8821 cacatgatta tctttaacgt acgtcacaat atgattatct ttctagggtt aatctagctg
     8881 cgtgttctgc agcgtgtcga gcatcttcat ctgctccatc acgctgtaaa acacatttgc
     8941 accgcgagtc tgcccgtcct ccacgggttc aaaaacgtga atgaacgagg cgcgctcact
     9001 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     9061 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     9121 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     9181 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     9241 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     9301 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     9361 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     9421 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     9481 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     9541 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     9601 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     9661 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     9721 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     9781 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     9841 gctgaagatc agttggg
//
